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Team:Paris Saclay/Notebook/August/1
From 2014.igem.org
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| - | Strains used: MG1655 and MG1655Z1, | + | Strains used: MG1655 and MG1655Z1, transformed the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/30 30th July], striated on 4 LB dishes per strain. |
===C - Salicylate Inducible Suppressing System=== | ===C - Salicylate Inducible Suppressing System=== | ||
Revision as of 09:51, 4 August 2014
Contents |
Friday 1st August
Lab work
A - The frame coli Odor free
Stria on LB dishes
by Romain
Strains used: MG1655 and MG1655Z1, transformed the 30th July, striated on 4 LB dishes per strain.
C - Salicylate Inducible Suppressing System
Liquid Culture
by Fabio
8 liquid cultures with 3ml of LB and 3µl of amp (at 10am - 8°C), all from the colonies made the 31st July. The colonies will be put at 37°C on sunday 3rd in order to have grown colonies on monday.
D - Lemon scent
Results of the Transformation of electrocompetent cells
by Arnaud & Terry
Transformation of electrocompetent cells made the July 29th. Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)
Results:
- Nothing has grown on the control dishes.
- Failure, nothing has grown on the dishes which contain DY330 with pJBE1
Members there:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Romain, Sean and Terry.
