Team:UT-Dallas/Notebook/8-01

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==August 1st, 2014==
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<h1 class="firstHeading" align="center">Friday, August 1st, 2014</h1>
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<center><p><a href="https://2014.igem.org/wiki/index.php?title=Team:UT-Dallas/Notebook/8-01&action=edit "style="color:#87A96B" >Click here to edit the page </a> </p></center>
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  <p> A relatively short day in the lab. We are in the process of fundamentally improving our cloning protocols after failure of 40 of our gRNA plates. </p>
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<p> On Saturday, Megan will come in to inoculate colonies from today's transformation of ctxB as well as the new chromoprotein plates. On Sunday, Sam will come to do a miniprep of all these parts.</P>
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      <th><font size="3">Today's tasks:</font></th>     
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<p>* Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight. </p>
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<p>* Miniprep tcfF-RBS-eYFP (PCR product)</p>
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<p>* Streak chromoprotein stab cultures from registry </P>
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      <br> We have iGEM-icecream today!.
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<p> Today's tasks </p>
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Revision as of 17:42, 1 August 2014

Friday, August 1st, 2014

Click here to edit the page

A relatively short day in the lab. We are in the process of fundamentally improving our cloning protocols after failure of 40 of our gRNA plates.

On Saturday, Megan will come in to inoculate colonies from today's transformation of ctxB as well as the new chromoprotein plates. On Sunday, Sam will come to do a miniprep of all these parts.
Today's tasks:

* Transform ligation from yesterday (Promoter-RBS-ctxB)plus negative and positive controls. Ligation was done at 16 degrees overnight.

* Miniprep tcfF-RBS-eYFP (PCR product)

* Streak chromoprotein stab cultures from registry



We have iGEM-icecream today!.

Retrieved from "http://2014.igem.org/Team:UT-Dallas/Notebook/8-01"