Team:ULB-Brussels/Safety

From 2014.igem.org

(Difference between revisions)
Line 14: Line 14:
<section style="text-align: centered; margin: 25px"><p>
<section style="text-align: centered; margin: 25px"><p>
To avoid an eventual contamination and apply the emergency mesures in the lab, </p>
To avoid an eventual contamination and apply the emergency mesures in the lab, </p>
-
deep attention is taken to use safely our lab equipement and our EColi bacteria. </p></section>
+
deep attention is taken to use safely our lab equipement and our E.Coli bacteria. </p></section>
-
<section style="text-align: centered; margin: 20px"><p>
+
We use only benign genes and strains of microorganisms (E. Coli K-12, M.C. 1061, S. Cervesiae,...) and the toxins we use are of no danger for humans. </p>
-
Because our system could be used to enhance the production of a dangerous protein, </p>
+
Furthermore,once our project will be finished, any bacterium that might escape</p>
-
gloves, glasses and coat are part of our usual equipment, </p>
+
will quickly die. Indeed, the antitoxin production is subordinate to an arabinose-dependant promoter. Thus,</p>
-
especially during electrophoresis. </p></section>
+
if it escapes the lab, the bacterium will no longer be provided with the inducer, and it will stop producing the antitoxin.</p></section>
-
 
+
-
The containment of the bacteria should not be a primary issue: </p>
+
-
Indeed, our project has a build-in biocontainment component. The antitoxin production is under </p>
+
-
the control of an arabinose-dependant promoter. If the bacteria escape from the bioreactor, they will no longer </p>
+
-
be provided with arabinose, thus they will stop producing the antitoxin and they will quickly die. </p>
+
 +
At last, Mighty Coli should ultimately be used in closed bioreactors, which also have their own built-in biocontainment component. We can thus safely assume that the Mighty Coli project does not comprehend any appreciable risk.</p>
-</section>
-</section>
<tr><td><br/><br/></td></tr>
<tr><td><br/><br/></td></tr>
</table></th></tr>
</table></th></tr>

Revision as of 12:06, 1 August 2014

$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}} \newcommand{\Stabi}{\small Stabi}$ $\newcommand{\EColi}{\small E.coli} \newcommand{\SCere}{\small S.cerevisae}\\[0cm] ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ \newcommand{\PI}{\small PI}$ $\newcommand{\Igo}{\Large\mathcal{I}} \newcommand{\Tgo}{\Large\mathcal{T}} \newcommand{\Ogo}{\Large\mathcal{O}} ~$ Example of a hierarchical menu in CSS

carousel slider

Retrieved from "http://2014.igem.org/Team:ULB-Brussels/Safety"




- Université Libre de Bruxelles -



Safety


To avoid an eventual contamination and apply the emergency mesures in the lab,

deep attention is taken to use safely our lab equipement and our E.Coli bacteria.

We use only benign genes and strains of microorganisms (E. Coli K-12, M.C. 1061, S. Cervesiae,...) and the toxins we use are of no danger for humans.

Furthermore,once our project will be finished, any bacterium that might escape

will quickly die. Indeed, the antitoxin production is subordinate to an arabinose-dependant promoter. Thus,

if it escapes the lab, the bacterium will no longer be provided with the inducer, and it will stop producing the antitoxin.

At last, Mighty Coli should ultimately be used in closed bioreactors, which also have their own built-in biocontainment component. We can thus safely assume that the Mighty Coli project does not comprehend any appreciable risk.

-