Team:Paris Saclay/Notebook/August/1

From 2014.igem.org

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(Results of the Transformation of electrocompetent cells)
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===D - Lemon scent===
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=Friday 1st August=
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==Lab work==
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===C - Salicylate Inducible Suppressing System===
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====Liquid Culture====
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''by Fabio''
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8 liquid cultures with 3ml of LB and 3µl of amp (at 10am - 8°C), all from the colonies made the [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/31#Bacterial_Culture 31st July]. The colonies will be put at 37°C on sunday 3rd in order to have grown colonies on monday.
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===D - Lemon scent===
====Results of the Transformation of electrocompetent cells====
====Results of the Transformation of electrocompetent cells====
''by Arnaud & Terry''
''by Arnaud & Terry''

Revision as of 10:04, 1 August 2014

Contents

Friday 1st August

Lab work

C - Salicylate Inducible Suppressing System

Liquid Culture

by Fabio

8 liquid cultures with 3ml of LB and 3µl of amp (at 10am - 8°C), all from the colonies made the 31st July. The colonies will be put at 37°C on sunday 3rd in order to have grown colonies on monday.

D - Lemon scent

Results of the Transformation of electrocompetent cells

by Arnaud & Terry

Transformation of electrocompetent cells made the July 29th. Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)

Results:

  • Nothing has grown on the control dishes.
  • Failure, nothing has grown on the dishes which contain DY330 with pJBE1


Members there:

  • Instructors and advisors: Alice, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Romain, Sean and Terry.

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