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Team:UCL/Diary/W2
From 2014.igem.org
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== Monday == | == Monday == | ||
==== <i>Lab meeting</i> ==== | ==== <i>Lab meeting</i> ==== | ||
| + | <ul> | ||
| + | <li> in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster! </li> | ||
| + | <li> monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks// </li> | ||
| + | <li> tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am). </li> | ||
| + | <li> tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A </li> | ||
| + | </ul> | ||
| + | |||
| + | ====Edo==== | ||
| + | Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed. | ||
==== Lewis M ==== | ==== Lewis M ==== | ||
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== Tuesday == | == Tuesday == | ||
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| - | |||
==== Lewis M ==== | ==== Lewis M ==== | ||
Revision as of 11:46, 28 July 2014
Contents |
Week 2
Monday
Lab meeting
- in lab this week: Bez (lead), Edo, Sanjay, Dave, (YK & Sohaila supervising) NO 4 headed monster!
- monday restriction double digest to be done for: 5 lisbon plasmids (cut EcoRI+XbaI/uncut), 8 biobricks (cut EcoRI+PstI/uncut), following NEB restriction double digest protocol. LacI and RBS digest necessary for ligation. LacI digested with EcoRI SpeI, RBS with XbaI and Pstl. NEB restriction digest protocol for biobrick assembly. //YK add hyperlinks//
- tuesday: run gel, visualization for all 28 lanes of digest, 2 lanes of PCR from last week (friday/wednesday?) //YK// before darren's meeting (11am).
- tbd when: sohaila and YK go through the SOP for the assays with adam and ning. hopefully start the assembly of LacI + RBS ( + AzoR + Terminator) #3A
Edo
Cried when I saw that my free trial of snapgene expired. Back to pen and paper to do the calculations. Sent an email for sponsorship, finger crossed.
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Adam
Kevin
Dave
Lewis B
Rob
Myriam
Alberto
Tuesday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Wednesday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Thursday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Friday
Lewis M
Sanjay
Maurice
Joy
Ning
Pamela
Bez
Daniel
Sohaila
Georgia
Yan-Kay
Tanel
Weekend
