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Team:Paris Saclay/Notebook/July/25
From 2014.igem.org
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After that, we made another electrophoresis which revealed successfully the purification. | After that, we made another electrophoresis which revealed successfully the purification. | ||
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===E - Salicylate Inducible Suppressing System=== | ===E - Salicylate Inducible Suppressing System=== | ||
Revision as of 20:38, 27 July 2014
Contents |
Friday 25th July
Lab work
C - Lemon scent
Results PCR targeting
by Romain
After the night, the electrophoresis revealed the oligonucleotides iPS70 and iPS71 prepared with the GoTaq enzyme.
We made a PCR Clean-up. Protocol
After that, we made another electrophoresis which revealed successfully the purification.
Preparation and Transformation of competent cells
E - Salicylate Inducible Suppressing System
Bacterial culture:
by Fabio
8 liquid cultures with 5ml of LB and 5µl of Amp, 4 with BBa_J61051 and 4 with BBa_K228001 (at 11am - 37°C - 150 rpm), all from the concentrated colony made the 24th July
Reunion
With Arnaud, Fabio, Sean, Terry, Alice, Solenne, Sylvie & Philippe
Members present:
- Instructors and advisors: Solenne.
- Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.
