Team:Paris Saclay/Notebook/July/24

From 2014.igem.org

(Difference between revisions)
(→Thursday 24th July)
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==Lab work==
==Lab work==
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===B - Construction of the fusion protein (color)===
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====Extraction====
===C - Lemon scent===
===C - Lemon scent===
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====Gel electrophoresis of p cola====
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[[File:LU000081.jpg|right]]
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''by Sean''
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p cola used: sample left overnight [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23 yesterday] resuspended in 30μl of H<sub>2</sub>O milliQ.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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'''Results'''
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#
====Results of the PCR Targeting====
====Results of the PCR Targeting====
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Protocol]
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===? - ?????===
 
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-
====Gel electrophoresis of p cola====
 
-
[[File:LU000081.jpg|right]]
 
-
''by Sean''
 
-
 
-
p cola used: sample left overnight [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23 yesterday] resuspended in 30μl of H<sub>2</sub>O milliQ.
 
-
 
-
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
 
-
 
-
'''Results'''
 
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-
#
 
'''Members present:'''
'''Members present:'''

Revision as of 08:10, 25 July 2014

Contents

Thursday 24th July

Lab work

B - Construction of the fusion protein (color)

Extraction

C - Lemon scent

Gel electrophoresis of p cola

LU000081.jpg

by Sean

p cola used: sample left overnight yesterday resuspended in 30μl of H2O milliQ.

Protocol

Results

Results of the PCR Targeting

by Romain

PCR targeting made the July 22nd.

Results:

  • Nothing has grown on the control dishes.
  • Failure, nothing has grown on the clone I dishes.
  • Failure, other bacteria have grown on the clone II dishes.

We have to make another PCR.

E - The Suppressing Salicylate System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).

Samples:

  1. BBa_J61051 Concentrated
  2. BBa_J61051 Undiluted
  3. BBa_K228001 Concentrated
  4. BBa_K228001 Undiluted
  5. DH5α Control

Protocol

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/24"