Team:Paris Saclay/Notebook/July/24

From 2014.igem.org

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(→Thursday 24th July)
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* Instructors and advisors: Alice and Solenne.
* Instructors and advisors: Alice and Solenne.
* Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.
* Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.
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===? - ?????===
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====Gel electrophoresis of p cola====
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''by Sean''
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p cola used: sample left overnight [https://2014.igem.org/Team:Paris_Saclay/Notebook/July/23 yesterday] resuspended in 30μl of H<2>O milliQ.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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Results
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]

Revision as of 13:15, 24 July 2014

Contents

Thursday 24th July

Lab work

C - Lemon scent

Results of the PCR Targeting

by Romain

PCR targeting made the July 22nd.

Results:

  • Nothing has grown on the control dishes.
  • Failure, nothing has grown on the clone I dishes.
  • Failure, other bacteria have grown on the clone II dishes.

We have to make another PCR.

E - The Suppressing Salicylate System

Transformation of competent E.coli cells

by Fabio

We transformed in DH5α both BioBricks rehydrated yesterday (BBa_J61051 and BBa_K228001).

Samples:

  1. BBa_J61051 Concentrated
  2. BBa_J61051 Undiluted
  3. BBa_K228001 Concentrated
  4. BBa_K228001 Undiluted
  5. DH5α Control

Protocol

Members present:

  • Instructors and advisors: Alice and Solenne.
  • Students: Arnaud, Fabio, Juliette, Pierre, Romain, Sean and Terry.

? - ?????

Gel electrophoresis of p cola

by Sean

p cola used: sample left overnight yesterday resuspended in 30μl of H<2>O milliQ.

Protocol

Results

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/24"