Team:Paris Saclay/Notebook/July/8

From 2014.igem.org

(Difference between revisions)
(→2 - Transformation in DH5α of the following biobricks:)
(→Tuesday 8th July)
Line 6: Line 6:
'''Sean'''
'''Sean'''
-
* BBa B0015(CmR)  
+
* BBa_B0015(CmR)  
-
* BBa J23119(CmR)
+
* BBa_J23119(CmR)
-
* BBa K731020(CmR)
+
* BBa_K731020(CmR)
-
* BBa K206000(CmR)
+
* BBa_K206000(CmR)
-
* BBa J04500(CmR)
+
* BBa_J04500(CmR)
-
* BBa J23100(AmpR)
+
* BBa_J23100(AmpR)
-
* BBa J23106(AmpR)
+
* BBa_J23106(AmpR)
-
* BBa J23114(AmpR)
+
* BBa_J23114(AmpR)
Line 19: Line 19:
# Pierce foil cover of well containing biobrick of interest.
# Pierce foil cover of well containing biobrick of interest.
-
# Add 10 μL of water in well.
+
# Add 10 μl of water in well.
-
# Transfer contents of well into a 1.5 mL microcentrifuge tube.
+
# Transfer contents of well into a 1.5 ml microcentrifuge tube.
====2 - Transformation in DH5α of the following biobricks:====
====2 - Transformation in DH5α of the following biobricks:====
'''Mathieu & Maher'''
'''Mathieu & Maher'''
-
* BBa B0015(CmR)
+
* BBa_B0015(CmR)
-
* BBa J23119(CmR)
+
* BBa_J23119(CmR)
-
* BBa K731020(CmR)
+
* BBa_K731020(CmR)
-
* BBa K206000(CmR)
+
* BBa_K206000(CmR)
-
* BBa J04500(CmR)
+
* BBa_J04500(CmR)
-
* BBa J23100(AmpR)
+
* BBa_J23100(AmpR)
-
* BBa J23106(AmpR)
+
* BBa_J23106(AmpR)
-
* BBa J23114(AmpR
+
* BBa_J23114(AmpR)
* Control + : pUC18 (20ng)
* Control + : pUC18 (20ng)
* Control -  
* Control -  

Revision as of 13:02, 24 July 2014

Contents

Tuesday 8th July

Lab work

1 - Rehydration of the following biobricks:

Sean

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)


Protocol:

  1. Pierce foil cover of well containing biobrick of interest.
  2. Add 10 μl of water in well.
  3. Transfer contents of well into a 1.5 ml microcentrifuge tube.

2 - Transformation in DH5α of the following biobricks:

Mathieu & Maher

  • BBa_B0015(CmR)
  • BBa_J23119(CmR)
  • BBa_K731020(CmR)
  • BBa_K206000(CmR)
  • BBa_J04500(CmR)
  • BBa_J23100(AmpR)
  • BBa_J23106(AmpR)
  • BBa_J23114(AmpR)
  • Control + : pUC18 (20ng)
  • Control -


Protocol

NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.

3 - Plasmid DNA preparation:

Romain & Sean

Plasmids used:

  • pJBEI-6409


E. coli strain used:

  • XL1-Blue

Protocol

Back to the calendar

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