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Team:Oxford/InterlabDevices
From 2014.igem.org
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| - | <p> | + | <p><font style="font-weight:bold">I. Using the DNA distribution kit to extract devices 1-3:</font></p> |
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| + | We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent E.coli cells (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for deivces 2a, 2b/3b, and 3a) using the <html><a href="https://2014.igem.org/Team:Oxford/protocols/Transformation_into_chemically_competent_E.coli">standard E.coli transformation protocol</a></html> (however, we used 200 uL of competent cells per transformation rather than 100uL). | ||
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Revision as of 11:16, 23 July 2014
Devices
I. Using the DNA distribution kit to extract devices 1-3:
We filled the 4 wells we needed with 10 uL of dH2O and let it sit for about 10 minutes to resuspend. We transformed the resuspended DNA into chemically competent E.coli cells (DH5) and plated them on an agar plate with kanamycin (plate for device 1) and chloramphenicol (plates for deivces 2a, 2b/3b, and 3a) using the standard E.coli transformation protocol (however, we used 200 uL of competent cells per transformation rather than 100uL).
