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Team:Paris Saclay/Notebook/July/22
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One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony. | One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony. | ||
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| + | ===4 - Plasmid DNA extraction=== | ||
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| + | ''by Sean and Terry'' | ||
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| + | Plasmids used: cf part 1 | ||
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| + | <span style="color:red">Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.</span> | ||
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'''Members there''': | '''Members there''': | ||
Revision as of 21:16, 22 July 2014
Contents |
Tuesday 22st July
Lab Work
1 - Samples for stock
by Fabio
We collected 1ml of each sample, added 240µl of glycerol (87%) and stored at -80°C.
- K1033902
- K1033905
- K1033910
- K1033913
- K1033922
- K1033925
- K1033927
- K1033929
- K103921
- K45017
- K731201
- K762100
from Liquide Bacterial Cultures transformed the 21st July
2 - Electrophoresis
by Arnaud (Process A), Fabio (gel), Marie and Romain (Process B)
We used 2µL of DNA of the following Biobricks in a 1% Agarose Gel.
Process A
Process B
Results:
- A:
- B:
- C:
3 - Bacterial Culture
by Fabio and Marie
One liquid culture with 5ml of LB (37°C - 150 rpm), IL1403 from the concentrated colony.
4 - Plasmid DNA extraction
by Sean and Terry
Plasmids used: cf part 1
Note: we accidentally used a buffer without 10% ethanol. After removal of the buffer, we repeated the step with a correct version. This may have affected the electrophoresis which followed.
Members there:
- Instructors and advisors: Solenne.
- Students: Arnaud, Fabio, Juliette, Mathieu, Marie, Pierre, Romain, Sean and Terry.
