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Team:Cambridge-JIC/Constructs/gal4 hap1gr H2RBmRFP
From 2014.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
<li>End date/time:</li> | <li>End date/time:</li> | ||
<li> UIDs of primers/plasmids used: | <li> UIDs of primers/plasmids used: | ||
| + | <ul> | ||
<li> A2, P12, P13</li> | <li> A2, P12, P13</li> | ||
<li> B3, P14, P13</li> | <li> B3, P14, P13</li> | ||
| - | <li> A4, P12, P13 </li> | + | <li> A4, P12, P13 </ul></li> |
<li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li> | <li><a href="http://pcrspreadsheet.com">PCR Spreadsheet (on google drive)</a></li> | ||
Revision as of 18:38, 22 July 2014
Gal4 - Hap1GR - H2BmRFP
Design
Aim of the construct
- Describe aims here
End goal plasmid
- insert image here
Experimentation (up to date)
PCR
Attempt 1:- Start date/time:
- End date/time:
- UIDs of primers/plasmids used:
- A2, P12, P13
- B3, P14, P13
- A4, P12, P13
- PCR Spreadsheet (on google drive)
- Gel 27/07/2014
Gibson
Attempt 1:- Start date/time:
- End date/time:
- Comments:
E-Coli transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Agrobacteria transformation
Attempt 1:- Start date/time:
- End date/time:
- Induce comments:
- Growth comments:
- Electroporation/selection comments:
Spore preparation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Spore transformation
Attempt 1:- Start date/time:
- End date/time:
- Comments:
Evaluation
- Start date/time:
- End date/time:
- Comments:
