Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 GFP Assay

(Difference between revisions)

Latest revision as of 15:31, 9 September 2015

Notebook

Lab Documents

Start
Double Transformation
- Sun Sep 7
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)

each of 2.5µL DNA to DH5α
Colony PCR
- Mon Sep 8
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃
Preculture
- Tue Sep 9
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)

LB 2 mL for 9hours

Turbidity was controlled 0.1
Culture
- Tue Sep 9
M9ZB 2 mL IPTG 20 µ or not

for 9 hours, 11 hours, 13hours
Assay
- Wed Sep 10
fluorescence imaging 488 nm 530 filter
Complete!

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 							<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
 							<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
-							<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Estimation">Evaluation</a></li>
+							<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
 						</ul>
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 </div>
 <div class="step-recipe">
-LB 2 mL for 9hours Turbidity was controlled 0.1
+LB 2 mL for 9hours
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+<div class="step-recipe">
+Turbidity was controlled 0.1
 </div>
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