Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 GFP Assay

From 2014.igem.org

(Difference between revisions)
 
(12 intermediate revisions not shown)
Line 5: Line 5:
{{Team:HokkaidoU_Japan/LargeDropDown/CSS}}
{{Team:HokkaidoU_Japan/LargeDropDown/CSS}}
<html>
<html>
 +
<link href='http://fonts.googleapis.com/css?family=Merriweather' rel='stylesheet' type='text/css'>
<div id="background">
<div id="background">
<!--begin header-->
<!--begin header-->
Line 38: Line 39:
<div class="ldd_submenu" style="left:-415px; width:970px;">
<div class="ldd_submenu" style="left:-415px; width:970px;">
<ul style="margin-left:20px;">
<ul style="margin-left:20px;">
-
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Introduction">Overview</a></li>
+
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Overview</a></li>
-
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Introduction">Introduction</a></li>
+
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Achievements">Achievements</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Projects/Overview/Achievements">Achievements</a></li>
Line 82: Line 82:
</ul>
</ul>
<ul>
<ul>
-
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High-School</a></li>
+
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey">High School</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Education">Education</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Survey#Survey">Survey</a></li>
Line 89: Line 89:
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_heading"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion">Discussion</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Background">Background</a></li>
-
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Estimation">Evaluation</a></li>
+
<li class="ldd_contents"><a href="https://2014.igem.org/Team:HokkaidoU_Japan/Outreach/Discussion#Evaluation">Evaluation</a></li>
</ul>
</ul>
</div>
</div>
Line 125: Line 125:
  <div class="step-tile">
  <div class="step-tile">
  <h2 class="step-title">
  <h2 class="step-title">
-
Transformation
+
Double Transformation
</h2>
</h2>
  </div>
  </div>
Line 138: Line 138:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-GFP-dt on pSB6A1 on ice  30min
+
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)
-
each of 2.5&micro;L DNA to DH5&alpha; 42&#8451;    30sec
+
</div>
-
on ice  2min
+
<div class="step-reipe">
 +
    each of 2.5&micro;L DNA to DH5&alpha;
 +
 
</div>
</div>
    </div>
    </div>
Line 161: Line 163:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1)
+
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1&#8451; & asB0034-R Tm:68.7&#8451;
-
pHN1257_up_F Tm:60.1&#8451;
+
 
-
asB0034-R Tm:68.7&#8451;
+
 
</div>
</div>
    </div>
    </div>
Line 184: Line 186:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-GFP-dT(pSB6A1)
+
Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)
-
LB 2 mL
+
</div>
-
for 9hours
+
<div class="step-recipe">
-
Turbidity was controlled 0.1
+
LB 2 mL for 9hours
 +
</div>
 +
<div class="step-recipe">
 +
Turbidity was controlled 0.1
</div>
</div>
    </div>
    </div>
Line 208: Line 213:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
M9ZB 2 mL
+
M9ZB 2 mL IPTG 20 &micro; or not
-
IPTG 20 &micro; or not
+
</div>
 +
<div class="step-recipe">
for 9 hours, 11 hours, 13hours
for 9 hours, 11 hours, 13hours
</div>
</div>
Line 231: Line 237:
      </ul>
      </ul>
      <div class="step-recipe">
      <div class="step-recipe">
-
fluoresence imageing 488 nm 530 filter
+
fluorescence imaging 488 nm 530 filter
</div>
</div>
    </div>
    </div>
Line 247: Line 253:
<div id="footer-wrapper" style="background-color: #d92424;">
<div id="footer-wrapper" style="background-color: #d92424;">
   <div id="footer-content">
   <div id="footer-content">
-
<div id="footer-logo">
+
    <div id="footer-logo">
-
<a href="http://igemhokkaidou.com/"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a>
+
      <a href="http://igemhokkaidou.wordpress.com"><img style="height:150px;position:relative;" src="https://static.igem.org/mediawiki/2014/3/39/HokkaidoU_logo_transparent.png"></a>
-
</div>
+
    </div>
-
<div id="footer-twitter">
+
    <div id="footer-twitter">
-
         <a href="http://twitter.com/igem_hokkaidou"><img style="height:150px; position:relative; bottom:0;" src="https://static.igem.org/mediawiki/2014/3/3b/HokkaidoU_Footer_Twitter.png"></a>
+
         <a href="http://twitter.com/igem_hokkaidou">
 +
        <img style="height:150px; position:relative; bottom:0;" src="https://static.igem.org/mediawiki/2014/3/3b/HokkaidoU_Footer_Twitter.png">
 +
        </a>
     </div>
     </div>
-
 
-
 
   </div>
   </div>
</div>
</div>

Latest revision as of 15:31, 9 September 2015

Notebook
Lab Documents

140910 asB0034 & B0034 GFP Assay

  • Start

  • Double Transformation

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)
    each of 2.5µL DNA to DH5α

  • Colony PCR

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-E1010-B0015(pSB6A1) pHN1257_up_F Tm:60.1℃ & asB0034-R Tm:68.7℃

  • Preculture

    Ptrc-stem-asB0034-stem(on pHN1257) & R0040-B0034-B0040-B0015(on pSB6A1)
    LB 2 mL for 9hours
    Turbidity was controlled 0.1

  • Culture

    M9ZB 2 mL IPTG 20 µ or not
    for 9 hours, 11 hours, 13hours

  • Assay

    fluorescence imaging 488 nm 530 filter

  • Complete!

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/asB0034/140910_asB0034%26B0034_GFP_Assay"