Team:Oxford/protocols/Growing Liquid Cell Cultures

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<h1>Growing Liquid Cell Cultures</h1><BR>
<h1>Growing Liquid Cell Cultures</h1><BR>
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<li>Switch on the lamina flow hood and carry out steps 2 and 3 in the hood.<BR><BR>
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<li>Pipette 5ml LB broth into a 15ml Falcon tube.<BR><BR>
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<li>Inoculate the Falcon tube with strain being grown using an inoculation loop.<BR><BR>
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<li>Grow at appropriate temperature (37°C for E. coli and 30°C for Pseudomonas) with shaking overnight. <BR><BR>
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Latest revision as of 16:18, 21 July 2014

Growing Liquid Cell Cultures


    ↩ Back to other protocols.

  1. Switch on the lamina flow hood and carry out steps 2 and 3 in the hood.

  2. Pipette 5ml LB broth into a 15ml Falcon tube.

  3. Inoculate the Falcon tube with strain being grown using an inoculation loop.

  4. Grow at appropriate temperature (37°C for E. coli and 30°C for Pseudomonas) with shaking overnight.

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