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| </div> | | </div> |
| </div> | | </div> |
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| + | |
| <div class= "row splashala"> | | <div class= "row splashala"> |
| <div class ="col-md-12 transp scroller"> | | <div class ="col-md-12 transp scroller"> |
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| <small>an iGEM team's tale by Aalto-Helsinki</small></h2> | | <small>an iGEM team's tale by Aalto-Helsinki</small></h2> |
| <p> | | <p> |
- | In March, we had 9 students who were excited about new field of science that was hardly known in Finland. No experience, some studies, but a lot of excitement. | + | In March, we had 9 students who were excited about a new field of science that was hardly known in Finland. No experience, some studies, but a lot of excitement. |
- | </p> | + | </p> |
| <p> | | <p> |
- | In October, we had developed a genetically engineered machine from scratch, made a mathemtical model and simulation based on it, ran our own research lab for 5 months, designed web applications to help research, built a pitch explaining synthetic biology to the general public, figured out a new way to do business and built a massive wiki documenting all of this. How did that happen? Check it out here. | + | In October, we had developed a genetically engineered machine from scratch, made a mathematical model and a simulation based on it, ran our own research lab for five months, designed web applications to make research easier, built a pitch explaining synthetic biology to the general public, figured out a new way to do business and built a massive wiki documenting all of this. How did that happen? Check it out here. |
- | </p> | + | </p> |
| <p> | | <p> |
| Our priorities during the summer were: | | Our priorities during the summer were: |
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| </p> | | </p> |
| <p> | | <p> |
- | We worked (at least) 8 hours a day from the middle of May until the end of August in the Otaniemi Science campus. There was a huge learning area in the building adjacent to our lab, so we made it our HQ during the summer. Then we kept on working as much possible in Autumn while continuing our studies. | + | We worked at least 8 hours a day from the middle of May until the end of August in Aalto University's science campus in Otaniemi. There was a huge learning hub/living room in the building adjacent to our lab, so we made it our HQ during the summer. Then we kept on working as much as possible in the autumn while continuing our studies. Some managed to pull off the same hours as before while still keeping up with their studies! |
- | </p>
| + | |
| </p> | | </p> |
| + | <blockquote> |
| + | It's a dangerous business, Frodo, going out your door. You step onto the road, and if you don't keep your feet, there's no knowing where you might be swept off to. |
| + | <cite>J.R.R. Tolkien, The Lord of the Rings</cite> |
| + | </blockquote> |
| </article> | | </article> |
| <div class="update There"></div> | | <div class="update There"></div> |
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| <div class="col-md-6 text-col-left"> | | <div class="col-md-6 text-col-left"> |
| <p> | | <p> |
- | All members of our team, who work in the lab, have had a safety training as a part of their studies. The training covered the following topics: | + | All of our team members who worked in the lab have had safety training as a part of their studies. The training covered the following topics: |
| </p> | | </p> |
| <ul> | | <ul> |
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| <h3>Our Laboratory</h3> | | <h3>Our Laboratory</h3> |
| <p> | | <p> |
- | We got to work in the Bioprocess Technology lab of Aalto University. We had our own table and we got to use all the instruments in the department. We also did some measurements in the labs of VTT Technical Research Centre of Finland. | + | We got to work in the Bioprocess Technology lab of Aalto University. We had our own lab bench and we got to use all the instruments and equipment in the department. We also did some measurements in the labs of the VTT Technical Research Centre of Finland. |
| </p> | | </p> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="col-md-3 feat-right"> | | <div class="col-md-3 feat-right"> |
- | <img src="https://static.igem.org/mediawiki/2014/b/b8/AaltoHelsinki_labplate.jpg" class="img-responsive"></img> | + | <img src="https://static.igem.org/mediawiki/2014/b/b8/AaltoHelsinki_labplate.jpg" class="img-responsive"></img> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="col-md-6 text-col-left"> | | <div class="col-md-6 text-col-left"> |
| <p> | | <p> |
- | We also needed a proper office space where we could work during the summer since not everyone was going to do lab work all the time (and there wasn't that much space in the lab either). | + | We also needed a proper office space where we could work during the summer since not everyone was going to do lab work and our lab bench wasn’t that spacious either. |
| </p> | | </p> |
| <p> | | <p> |
- | We managed to find the "Learning Hub Living Room (CHEM)" (Vuori Hub) in the building of Department of Materials Science and Engineering. Since it was summer, there weren't many people around to use the space and we made it our home. It was a comfortable place to work in! | + | We managed to find the "Learning Hub Living Room" (Vuori Hub) in the building of Department of Materials Science and Engineering. Since it was summer, there weren't many people around to use the space and we made it our home. It was a comfortable place to work in! |
| </p> | | </p> |
| </div> | | </div> |
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| | | |
- |
| + | |
| </article> | | </article> |
| | | |
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| </p> | | </p> |
| <ul> | | <ul> |
- | <li><a href="">May-June (lab)</a></li> | + | <li><a href="https://static.igem.org/mediawiki/2014/6/67/AaltoHelsinki_Lab_Notebook_May-June.pdf">May-June (lab)</a></li> |
- | <li><a href="">July (lab)</a></li> | + | <li><a href="https://static.igem.org/mediawiki/2014/b/b2/AaltoHelsinki_Lab_Notebook_July.pdf">July (lab)</a></li> |
- | <li><a href="">August (lab)</a></li> | + | <li><a href="https://static.igem.org/mediawiki/2014/f/fc/AaltoHelsinki_Lab_Notebook_August.pdf">August (lab)</a></li> |
- | <li><a href="">September-October (lab)</a></li> | + | <li><a href="https://static.igem.org/mediawiki/2014/e/ee/AaltoHelsinki_Lab_Notebook_September-October.pdf">September-October (lab)</a></li> |
| </ul> | | </ul> |
| <br> | | <br> |
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| </ol> | | </ol> |
| <p> | | <p> |
- | Each of us wrote the answers down on our online team tool (Flowdock). According to the answers, we then assembled a detailed description of every single working day during our official work months (from May 20th to the end of August). So if you really really want to read exactly what we did, here's 30 pages of text for you to check out: | + | Each of us wrote the answers down on our online team chat tool (Flowdock). According to the answers, we then assembled a detailed description of every single working day during our official work months (from May 20th to the end of August). So if you really really want to read exactly what we did, here's 30 pages of text for you to check out: |
| </p> | | </p> |
| <ul> | | <ul> |
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| <h3>The Actual Timeline</h3> | | <h3>The Actual Timeline</h3> |
| <p> | | <p> |
- | We gathered the highlights of our project to the timeline below so you can effortlessly skim through the story of Aalto-Helsinki Bioworks! Lab things are on the right side, office stuff (and everything else) on the left. | + | We gathered the highlights of our project to the timeline below so you can effortlessly skim through the story of Aalto-Helsinki Bioworks! Lab things are on the right side and everything else on the left. |
| </p> | | </p> |
| + | <p>Timeline reference links:</p> |
| + | <ul> |
| + | <li><a href="#November">November</a></li> |
| + | <li><a href="#October">October</a></li> |
| + | <li><a href="#September">September</a></li> |
| + | <li><a href="#August">August</a></li> |
| + | <li><a href="#July">July</a></li> |
| + | <li><a href="#June">June</a></li> |
| + | <li><a href="#May">May</a></li> |
| + | <li><a href="#April">April</a></li> |
| + | <li><a href="#March">March</a></li> |
| + | <li><a href="#Beginning">The Beginning</a></li> |
| + | </ul> |
| </article> | | </article> |
| <ul class="timeline"> | | <ul class="timeline"> |
- | | + | <li class="month" id="November"><div class="tldate">November 2014</div></li> |
- | <li class="month"><div class="tldate">October 2014</div></li> | + | <li class="entry"> |
- | | + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Best Wiki prize!</h4> |
| + | <p><small class="text-muted">3.11.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p> |
| + | Amazing!! This wiki won the prize for the best undergraduate wiki! We're so happy to bring a prize to home as the first-ever Finnish team. Good job everybody and big thanks to the people who have been helping us getting there! |
| + | </p> |
| + | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/thumb/1/10/Aaltohelsinki_prize.jpg/800px-Aaltohelsinki_prize.jpg" class="img-responsive"> |
| + | </div> |
| + | </li> |
| + | <li class="month" id="October"><div class="tldate">October 2014</div></li> |
| + | <li class="entry"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Giant Jamboree in Boston</h4> |
| + | <p><small class="text-muted">30.10.-3.11.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>Flights from Helsinki to Boston on 27th October. A full week in Boston. Presenting our project in front of thousands of people.</p> |
| + | </div> |
| + | </div> |
| + | </li> |
| + | <li class="entry"> |
| + | <div class="timeline-panel"> |
| + | <div class="tl-heading"> |
| + | <h4>Bootcamp Weekend</h4> |
| + | <p><small class="text-muted">25.10.2014</small></p> |
| + | </div> |
| + | <div class="tl-body"> |
| + | <p>Perfecting everything and admiring our work. All is well and we can just relax before the Jamboree.</p> |
| + | </div> |
| + | </div> |
| + | </li> |
| <li class="entry"> | | <li class="entry"> |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Jamboree Bootcamp at Mikkeli Summer Cottage</h4> | + | <h4>Presentation at HYBER Workshop</h4> |
- | <p><small class="text-muted"> 25.10.2014</small></p> | + | <p><small class="text-muted">24.10.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Perfecting everything and admiring our wiki which is totally done at this point. All's well, we can just relax before the Jamboree.</p> | + | <p>A presentation for HYBER Workshop in Långvik; great practice for the Giant Jamboree!</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="tl-heading"> | | <div class="tl-heading"> |
| <h4>Furiously Finishing the Wiki</h4> | | <h4>Furiously Finishing the Wiki</h4> |
- | <p><small class="text-muted"> 8.10.-17.10.2014</small></p> | + | <p><small class="text-muted">8.10.-17.10.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Unfortunately, due to time running out we had to freeze the lab work. We couldn't quite finish our wet lab project. Still, after almost 5 months of continuous work in the lab, we can be proud of what we've achieved and learned during this project!</p> | + | <p>Unfortunately, due to time running out we had to freeze the lab work and we couldn't quite finish our wet lab project. Still, after almost five months of continuous work in the lab, we can be proud of what we've achieved and learned during this project!</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We didn’t have time to sequence our new Biobricks before sending them to iGEM HQ but we wanted to sequence them anyway, just in case. The sequences were confirmed to be ok! We were really happy about this since we did have some doubts about synthesizing parts with PCR on our own.</p> | + | <p>We didn’t have time to sequence our new Biobricks before sending them to iGEM HQ but we wanted to sequence them anyway, just in case. The sequences were confirmed to be ok! We were really happy about this since we did have some doubts about making parts with PCR on our own.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Finally we got our own Biobricks ready to be sent to iGEM HQ. We put them in a box and wished them a safe journey.</p> | + | <p>Finally our own Biobricks were ready to be sent to iGEM HQ. We put them in a box and wished them a safe journey.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/b/b3/AaltoHelsinki_shipment.JPG" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/b/b3/AaltoHelsinki_shipment.JPG" class="img-responsive"> |
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| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">September 2014</div></li> | + | <li class="month" id="September"><div class="tldate">September 2014</div></li> |
| | | |
| <li class="entry"> | | <li class="entry"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Out of nowhere we were invited to do an interview for the national radio YLE! We talked about our project and showed the interviewer around our lab. | + | <p>Out of nowhere we were invited to be interviewed for the national radio YLE! We talked about our project and showed the interviewer our lab. |
| </p> | | </p> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We had a lot of problems with one specific ligation. Every time time we tried, only one of the inserts seemed to ligate with the backbone and the PCR and restriction digestion results on the gel looked bizarre.</p> | + | <p>We had a lot of problems with one specific ligation. Every time we tried, only one of the inserts seemed to ligate with the backbone and moreover the PCR and restriction digestion results on the gel looked bizarre.</p> |
- | <p>For the longest time we pondered what could be wrong with our protocols or parts but then with the help of a professor we figured out that the problem might be that we had repetetive sequences in both inserts. Apparently <em>E. coli</em> doesn't like having two similar sequences in the same plasmid and it splices the other one out.</p> | + | <p>For the longest time we pondered what could be wrong with our protocols or parts but then with the help of a professor we figured out that the problem might be that we had repetitive sequences in both inserts. Apparently <em>E. coli</em> doesn't like having two similar sequences in the same plasmid and it splices the other one out.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="tl-heading"> | | <div class="tl-heading"> |
| <h4>Juggling Between Lab and School</h4> | | <h4>Juggling Between Lab and School</h4> |
- | <p><small class="text-muted">8.10.2014</small></p> | + | <p><small class="text-muted">8.9.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>At this point everyone already had courses going but luckily the lab is right upstairs form the lecture halls so we could still continue working almost full time in the lab. We just needed to time everything perfectly so we could go listen to the lectures while we had incubations or PCRs running.</p> | + | <p>At this point everyone already had courses going but luckily the lab is right upstairs from the lecture halls so we could still continue working almost full time in the lab. We just needed to time everything perfectly so we could go listen to the lectures while we had incubations or PCRs running.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We held a presentation about our project at VTT. It was the first time we presented ourselves to a real scienctific audience and we got some really good feedback from the researchers.</p> | + | <p>We held a presentation about our project at VTT. It was the first time we presented ourselves to a real scientific audience and we got some really good feedback from the researchers.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We participated in an event called Pitch Night and Oskari gave an inspiring speech about following dreams and introduced our project to the audience which mostly consisted of middle-aged people. Pitch Night was part of the Thinkfest arranged by University of Helsinki in celebration of the university's 375th anniversary.</p> | + | <p>Pitch Night was part of the Thinkfest arranged by the University of Helsinki in celebration of the university's 375th anniversary. Oskari gave an inspiring speech about following your dreams and introduced our project to the audience which mostly consisted of middle-aged people.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>To kick off the academic year, Aalto University hosts a party every year during the first week of school. Despite the name, it's not really a party but more of an exhibition to show all the cool stuff the university has to offer. Of course, we were there to represent our team and to tell people about iGEM and our project.</p> | + | <p>To kick off the academic year, Aalto University hosts a party every year during the first week of school. Despite the name, it's not really a party but more of an exhibition to show all the cool stuff the university has to offer. We were there to represent our team and to tell people about iGEM and our project.</p> |
| </div> | | </div> |
| </div> | | </div> |
| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">August 2014</div></li> | + | <li class="month" id="August"><div class="tldate">August 2014</div></li> |
| | | |
| <li class="entry timeline-inverted"> | | <li class="entry timeline-inverted"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We had another round of testing with a part of our prototype and the LEDrig. The results seemed really promising!</p> | + | <p>We had another round of testing with a part of our prototype and the LED rig. The results seemed really promising!</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We planned to go to an escape the room thing, but when all the suitable times are booked, Minttu took us bowling instead.</p> | + | <p>We planned to go to a room escape thing but when all the suitable times were booked, Minttu took us bowling instead.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p> We went to visit the observatorium during the Night of the Arts event in Helsinki. There were also massive soap bubbles and a man with a whole band setup in a baby carriage.</p> | + | <p> We went to visit the observatory during the Night of the Arts event in Helsinki. There were also massive soap bubbles and a man with a whole band setup in a baby carriage.</p> |
| | | |
| <p> The next day we went back to playing pinball and Crash Team Racing because it was so much fun last week.</p> | | <p> The next day we went back to playing pinball and Crash Team Racing because it was so much fun last week.</p> |
| + | <img src="https://static.igem.org/mediawiki/2014/b/b1/Aalto_Helsinki_Pinball.jpg" class="img-responsive"> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Our Synthetized Genes Arrived!</h4> | + | <h4>Our Synthesized Genes Arrived!</h4> |
| <p><small class="text-muted">18.8.2014</small></p> | | <p><small class="text-muted">18.8.2014</small></p> |
| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>After a long while, our genes finally arrived. Now we could take them and other parts we had assembled and put the whole genetically engineered machine together.</p> | + | <p>After a long while, our genes finally arrived. Now we could take them and other parts we had assembled and put the whole ingenious genetically engineered machine together.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We met the event manager and event organizer of Heureka and came up with a synthetic biology weekend workshop we could organize in Spring.</p> | + | <p>We met the event manager and event organizer of Heureka and came up with an idea of a synthetic biology weekend workshop we could organize in the spring.</p> |
| | | |
- | <p>They were very excited about us and everything we had to show and tell. They said that this is a perfect match for them and all the simulations, games, pictures fit perfectly into the workshop.</p> | + | <p>They were very excited about us and everything we had to show and tell.</p> |
| | | |
- | <p>We hope to inspire young people to study synthetic biology in the future and let the older people know it exists. This will also be a perfect opportunity for the next year's iGEM Team to get involved and organize something together.</p> | + | <p>We hope to inspire young people to study synthetic biology in the future and let the older people know it exists.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <p>Lassi took us to play pinball. It was surprisingly fun. We played a ton of Crash Team Racing too.</p> | | <p>Lassi took us to play pinball. It was surprisingly fun. We played a ton of Crash Team Racing too.</p> |
| </div> | | </div> |
| + | <img src="https://static.igem.org/mediawiki/2014/2/24/Aalto_Helsinki_CTR.jpg" class="img-responsive center-block" id="tlsmallimg"> |
| </div> | | </div> |
| </li> | | </li> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Pietu had been busy making the LEDrig inspired by Tabor Lab. This is what the early version looked like. The LEDrig would be the tool to control our light sensitive bacteria.</p> | + | <p>Pietu had been busy making the LED rig inspired by Tabor Lab. This is what the early version looked like. The LED rig would be the tool to control our light sensitive bacteria.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/7/73/AaltoHelsinki_earlyLEDrig.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/7/73/AaltoHelsinki_earlyLEDrig.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We met with The Academy of Finland's Center of Excellence HYBER 2014-2019. They wanted us to talk in their conference and support us. </p> | + | <p>We met with The Academy of Finland's Center of Excellence HYBER 2014-2019. They wanted to support us and invited us to talk in workshop that would be arranged just before the Giant Jamboree.</p> |
| | | |
| <p>In the evening Oskari took us to play laser tag! He was way better at it than anyone else.</p> | | <p>In the evening Oskari took us to play laser tag! He was way better at it than anyone else.</p> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We got our first sequencing results that were in confusing .ab1-files. Then it turned out that Geneious makes analysing them incredibly easy. Most of the sequences looked good, some not so much.</p> | + | <p>We got our first sequencing results that were in confusing .ab1-files. Then it turned out that Geneious program makes analysing them incredibly easy. Most of the sequences looked good, some not so much.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">July 2014</div></li> | + | <li class="month" id="July"><div class="tldate">July 2014</div></li> |
| | | |
| <li class="entry"> | | <li class="entry"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We built the sequences to synthetize from scratch. They included the lambda repressor <em>O</em><sub>R</sub> sites that we designed. We found out that the synthetisizing costs the same for 0-1000 nucleotides, so we include a few surrounding BioBricks to save on lab time. (The <em>O</em><sub>R</sub> sites are fairly small.)</p> | + | <p>We assembled the sequences we wanted to synthesize. They included the lambda repressor <em>O</em><sub>R</sub> sites that we designed. We found out that the synthesizing costs the same for 0-1000 nucleotides, so we included a few surrounding BioBricks to save on lab time. (The <em>O</em><sub>R</sub> sites are fairly small.)</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We had a number of ligations ready for assembling and it turned out there was a sequencing lab like 5 kilometers away.</p> | + | <p>We had a number of ligations ready for assembling and it turned out there was a sequencing lab about 5 kilometers away.</p> |
| <p>We sealed 16 samples into tubes and sent them off. We were left eagerly awaiting to see if the work this far had succeeded.</p> | | <p>We sealed 16 samples into tubes and sent them off. We were left eagerly awaiting to see if the work this far had succeeded.</p> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p> We meet Tania, an ex-iGEMer from the <a href="https://2012.igem.org/Team:Amsterdam/team/members">2012 Amsterdam team.</a> It was a great meeting and we finally got some validation that we're doing some things right.</p> | + | <p> We met Tania, an ex-iGEMer from the <a href="https://2012.igem.org/Team:Amsterdam/team/members">2012 Amsterdam team.</a> It was a great meeting and we finally got some validation that we're doing some things right.</p> |
- | <p>In the evening Martina took us geocaching! It is amazing what can be hidden in completely plain sight.</p> | + | <p>In the evening Martina took us geocaching! It is amazing what can be hidden completely in plain sight.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>The new primers designed by Lassi arrived and we tested them in amplifying the backbones with PCR. The primers bind to the RFP insert instead of the prefix and suffix which have a palindromic site and can cause troubles. In the end we didn't use PCR amplification of backbones since we opted for gel purifying instead. However, we did submit the primers to the registry and you can find them here <a href="http://parts.igem.org/Part:BBa_K1443003">BBa_K1443003</a> and <a href="http://parts.igem.org/Part:BBa_K1443004">BBa_K1443004</a>.</p> | + | <p>The new primers designed by Lassi arrived and we tested them in amplifying the backbones with PCR. The primers bind to the RFP insert instead of the prefix and suffix which have a palindromic site and can cause troubles. In the end we didn't use PCR amplification of the backbones since we opted for multiplying the whole plasmids in bacteria and gel purifying them instead. However, we did submit the primers to the Registry and you can find them here <a href="http://parts.igem.org/Part:BBa_K1443003">BBa_K1443003</a> and <a href="http://parts.igem.org/Part:BBa_K1443004">BBa_K1443004</a>.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/2/2f/AaltoHelsinki_primers.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/2/2f/AaltoHelsinki_primers.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>In the evening Mikko took us to watch The Skin I Live In, Scott Pilgrim vs. The World and The Grand Budapest Hotel.</p> | + | <p>In the evening Mikko took us to watch the movies <i>The Skin I Live In</i>, <i>Scott Pilgrim vs. The World</i> and <i>The Grand Budapest Hotel</i>.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We had the lambda repressor figured out and integrated into our system. We started planning for the parts to be synthesized.</p> | + | <p>We finished the gene circuit and now we could start designing the parts that would be synthesized.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/a/a5/Aalto_Helsinki_loop.png" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/a/a5/Aalto_Helsinki_loop.png" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Summer of Startups took us to visit the Startup people of Tallinn. We decided this was a practice trip for Boston, so we planned to have as much fun as possible. It was a great success and an unforgettable experience.</p> | + | <p>Summer of Startups took us to visit the startup people of Tallinn. We decided this was a practice trip for Boston, so we planned to have as much fun as possible. It was a great success and an unforgettable experience.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/f/f9/Aalto_Helsinki_Tallinna.jpg" class="img-responsive"> | | <img src="https://static.igem.org/mediawiki/2014/f/f9/Aalto_Helsinki_Tallinna.jpg" class="img-responsive"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We decided that as a group we should do something fun every week that has nothing to do with iGEM. Every team member had their own week to plan an activity and pull the team to the mandatory weekly having-fun moment.</p> | + | <p>We decided we should do something (not iGEM-related) fun every week as a group. Each team member had their own week to plan an activity and pull the team to the mandatory weekly having-fun moment.</p> |
| | | |
| <p>So, Otto surprised us with a fruit juicer and a ton of fresh fruit. We spent the early morning happily drinking smoothies while working intensely on our project.</p> | | <p>So, Otto surprised us with a fruit juicer and a ton of fresh fruit. We spent the early morning happily drinking smoothies while working intensely on our project.</p> |
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| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Fresh Meat in the Lab</h4> | + | <h4>A N00b in the Lab</h4> |
| <p><small class="text-muted">3.7.2014</small></p> | | <p><small class="text-muted">3.7.2014</small></p> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>Because chimera is a term both in gene techonology and mythology, Laura decided (and others immediately agreed) that drawing a chimera for each member would be a cool idea. Everyone could decide three animals and a chimera would be drawn based on those.</p> | + | <p>Because chimera is a term both in gene technology and mythology, Laura decided (and others immediately agreed) that drawing a chimera for each member would be a cool idea. Everyone could decide three animals and a chimera would be drawn based on those.</p> |
| | | |
| <p>To read more about the chimeras, head to <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach#chimeras">"chimeras" section on the Outreach page</a>.</p> | | <p>To read more about the chimeras, head to <a href="https://2014.igem.org/Team:Aalto-Helsinki/Outreach#chimeras">"chimeras" section on the Outreach page</a>.</p> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We got the idea of explaining synthetic biology as turning bacteria into superheroes from a meeting with Miki Honkavaara. We started working on a new pitch based on that.</p> | + | <p>We got the idea of explaining synthetic biology as turning bacteria into superheroes from a meeting with Micki Honkavaara. We started working on a new pitch based on that.</p> |
| | | |
| <p>Pietu finished the pitch the next day and presented it at Summer of Startups. It seemed to go over very well. People finally understood what we were doing.</p> | | <p>Pietu finished the pitch the next day and presented it at Summer of Startups. It seemed to go over very well. People finally understood what we were doing.</p> |
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| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">June 2014</div></li> | + | <li class="month" id="June"><div class="tldate">June 2014</div></li> |
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| <li class="entry"> | | <li class="entry"> |
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| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We kept getting weird results from PCR so we tried to solve the issue by doing a gradient PCR with different annealing temperatures. Seemed like 55 °C would be the answer to our problems.</p> | + | <p>We kept getting weird results from PCR so we tried to solve the issue by doing a gradient PCR with different annealing temperatures. It seemed like 55 °C would be the answer to our problems.</p> |
| </div> | | </div> |
| <img src="https://static.igem.org/mediawiki/2014/2/27/AaltoHelsinki_gradientPCR.jpg" class="img-responsive center-block" id="tlsmallimg"> | | <img src="https://static.igem.org/mediawiki/2014/2/27/AaltoHelsinki_gradientPCR.jpg" class="img-responsive center-block" id="tlsmallimg"> |
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| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>First Colony PCR: No Succesful Ligations</h4> | + | <h4>First Colony PCR: No Successful Ligations</h4> |
| <p><small class="text-muted">11.6.2014</small></p> | | <p><small class="text-muted">11.6.2014</small></p> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We were trying to advertise ourselves and get some kind of financial help and support for this project. It felt really difficult and time consuming. We also took some new pictures, a new group photo, for example.</p> | + | <p>We were trying to advertise ourselves and get some kind of financial help and support for this project. It felt really difficult and time consuming. We also took some new pictures, a new group photo, for example.</p> |
| </div> | | </div> |
- | <img src="http://aaltohelsinki.com/img/igem_aalto_helsinki_2014.jpg" class="img-responsive"></img> | + | <img src="https://static.igem.org/mediawiki/2014/7/73/Aalto_Helsinki_Team.jpg" class="img-responsive"></img> |
| </div> | | </div> |
| </li> | | </li> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>The backbones did't seem to be working right and the negative control kept growing. Not good.</p> | + | <p>The backbones didn't seem to be working right and the negative control kept growing. Not good.</p> |
| <p>The reason seemed to be that we hadn’t gel purified the backbone and it kept religating with itself.</p> | | <p>The reason seemed to be that we hadn’t gel purified the backbone and it kept religating with itself.</p> |
| </div> | | </div> |
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| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">May 2014</div></li> | + | <li class="month" id="May"><div class="tldate">May 2014</div></li> |
| | | |
| <li class="entry timeline-inverted"> | | <li class="entry timeline-inverted"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>This process would be repeated about 50 times during the summer, but this was the first. Video link: <a href="http://www.youtube.com/watch?v=TI_EuzNIaPg">http://www.youtube.com/watch?v=TI_EuzNIaPg</a>.</p> | + | <p>This process would be repeated about 50 times during the summer, but this was the first.<a href="http://www.youtube.com/watch?v=TI_EuzNIaPg"><img src="https://static.igem.org/mediawiki/2014/f/f5/Aalto_Helsinki_Cell_Membrane_Video.png" class="img-responsive"></a>.</p> |
| </div> | | </div> |
| </div> | | </div> |
| </li> | | </li> |
- |
| |
| | | |
| <li class="entry"> | | <li class="entry"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>It started from the mild inconvenience of searching for BioBricks in the lab. Now we could search through this year's distribution effortlessly. Because it was so useful for us, we decided to share it to others, too.</p> | + | <p>It started from the mild inconvenience of searching for BioBricks from four different excel sheets in the lab. At this point, the Registry pages didn't yet have the updated info about part availability either. With the tool we could search through this year's distribution effortlessly. Because it was so useful for us, we decided to share it to others, too.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>This time it did actually look cool! The actual website might still be available on <a href="http://aaltohelsinki.com/index_en.html">http://aaltohelsinki.com/index_en.html</a>: check it out!</p> | + | <p>This time it did actually look cool! The actual website might still be available on <a href="http://2014.aaltohelsinki.com/">http://2014.aaltohelsinki.com/</a>. Check it out!</p> |
| </div> | | </div> |
| </div> | | </div> |
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| <p>We got the eagerly awaited box from iGEM and the competition started to feel real. In the box we found the Distribution Kit plates full of Biobricks and other cool stuff like these pins!</p> | | <p>We got the eagerly awaited box from iGEM and the competition started to feel real. In the box we found the Distribution Kit plates full of Biobricks and other cool stuff like these pins!</p> |
| </div> | | </div> |
- | <img src="https://static.igem.org/mediawiki/2014/0/05/AaltoHelsinki_pinssi.jpg" class="img-responsive"> | + | <img src="https://static.igem.org/mediawiki/2014/0/05/AaltoHelsinki_pinssi.jpg" class="img-responsive center-block" id="tlsmallimg"> |
| </div> | | </div> |
| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">April 2014</div></li> | + | <li class="month" id="April"><div class="tldate">April 2014</div></li> |
| | | |
| <li class="entry"> | | <li class="entry"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We met a few times to go over what we would say to the Summer of Startups panel, so that they would accept us to the program. This culminated on the 28th, when we had the interview. Our team leader tried to attend through Skype, but the panel didn't like it. We managed to pull through anyway, and the panel was very excited about us at the end.</p> | + | <p>We met a few times to go over what we would say to the Summer of Startups panel, so that they would accept us to the program. This culminated on the 28th, when we had the interview. Our team leader tried to attend through Skype, but the panel didn't like it. We managed to pull through anyway, and the panel was very excited about us in the end.</p> |
| <p>They accepted us in the program.</p> | | <p>They accepted us in the program.</p> |
| </div> | | </div> |
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| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">March 2014</div></li> | + | <li class="month" id="March"><div class="tldate">March 2014</div></li> |
| | | |
| <li class="entry"> | | <li class="entry"> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>We made an introduction video of us so we could apply to Summer of Startups. It turned out pretty good! Video link: <a href="http://www.youtube.com/watch?v=LJX21WthZQg">http://www.youtube.com/watch?v=LJX21WthZQg</a>.</p> | + | <p>We made an introduction video of us so we could apply to the Summer of Startups incubator program. It turned out pretty good! Video link: <a href="http://www.youtube.com/watch?v=LJX21WthZQg">http://www.youtube.com/watch?v=LJX21WthZQg</a>.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
| <div class="tl-body"> | | <div class="tl-body"> |
- | <p>The first team meeting was held on a Friday afternoon. Some of us met each other for the first time ever, some had met before already. We talked about the project and got to know each other.</p> | + | <p>The first team meeting was held on a Friday afternoon. Some of us met each other for the first time ever, some had already met before. We talked about the project and got to know each other.</p> |
| </div> | | </div> |
| </div> | | </div> |
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| </li> | | </li> |
| | | |
- | <li class="month"><div class="tldate">The Beginning</div></li> | + | <li class="month" id="Beginning"><div class="tldate">The Beginning</div></li> |
| | | |
| <li class="entry"> | | <li class="entry"> |
| <div class="timeline-panel"> | | <div class="timeline-panel"> |
| <div class="tl-heading"> | | <div class="tl-heading"> |
- | <h4>Meetings with Aalto University and University of Helsinki</h4> | + | <h4>Meetings with Aalto University and the University of Helsinki</h4> |
| <p><small class="text-muted">31.1.2014, 5.2.2014</small></p> | | <p><small class="text-muted">31.1.2014, 5.2.2014</small></p> |
| </div> | | </div> |