Team:NJAU China

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
 
(174 intermediate revisions not shown)
Line 1: Line 1:
-
{{CSS/Main}}
+
<html lang="en">
 +
<head>
 +
    <meta charset="utf-8">
 +
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
 +
    <meta name="viewport" content="width=device-width, initial-scale=1">
 +
    <meta name="description" content="">
 +
    <meta name="author" content="">
 +
    <link rel="icon" href="../../favicon.ico">
 +
    <title>Home</title>
 +
    <script src="https://2014.igem.org/Team:NJAU_China/jquery.min.js?action=raw"></script>
 +
    <script src="https://2014.igem.org/Team:NJAU_China/bootstrap.min.js?action=raw"></script>
 +
    <link rel="stylesheet" type="text/css"
 +
          href="https://2014.igem.org/Team:NJAU_China/bootstrap.min.css?action=raw&amp;ctype=text/css">
 +
    <link rel="stylesheet" type="text/css"
 +
          href="https://2014.igem.org/Team:NJAU_China/bootstrap-theme.min.css?action=raw&amp;ctype=text/css">
 +
    <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
 +
    <!--[if lt IE 9]>
 +
    <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
 +
    <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
 +
    <![endif]-->
-
<html>
+
    <!-- Custom styles for this template -->
 +
    <link href="carousel.css" rel="stylesheet">
 +
    <style type="text/css">
 +
        body {
 +
            padding-bottom: 40px;
 +
            background:#a2dcf3;
 +
            color: #5a5a5a;
 +
            font-size: 14px;
 +
            font-family:Andalus;
 +
        }
 +
        #myheader{
 +
            width:1366px;
 +
    height:500px;
 +
    margin:0 auto;
 +
    position:relative;
 +
    font-family:Arial, Helvetica, sans-serif;
 +
            background:#a2dcf3 url("https://static.igem.org/mediawiki/2014/3/34/Header_bg.png");
 +
        }
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
 +
            display: none;
 +
        }
-
<!--main content -->
+
        /*-- hides default wiki settings --*/
-
<table width="70%" align="center">
+
        .firstHeading {
 +
            width: 975px;
 +
            margin: 0px auto;
 +
            padding-top: 100px;
 +
            margin-bottom: 20px;
 +
            font-family: Georgia, Times, "Andalus", serif;
 +
        }
 +
        h1, h2, h3, h4, h5 {font-family: Georgia, Times, "Andalus", serif;}
 +
        #top-section {
 +
            /*-- styling for default menu bar (edit, page, history, etc.) --*/ background-color: #383838;border: 0 none;height: 14px;z-index: 100;top: 0;position: fixed; width: 975px;left: 50%;margin-left: -487px;}
 +
#top-section-bar {
 +
            /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
 +
  background-color: #383838;
 +
  height: 14px;
 +
  display: block;
 +
  z-index: 10;position: fixed; width: 100%; top: 0;}
 +
  body { background-color: #fff;
 +
  }
 +
#globalWrapper, #content {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  border: 0px;
 +
  background-color: transparent;
 +
  margin: 0px;
 +
  padding: 0px;
 +
  }
-
<!--welcome box -->
+
  html, body, .wrapper {
-
<tr>
+
  /*-- changes default wiki settings --*/
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
  width: 100%;
-
<h1 >WELCOME TO iGEM 2014! </h1>
+
  height: 100%;
-
<p>Your team has been approved and you are ready to start the iGEM season!
+
  background-color: transparent;
-
<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
+
  } #container {
-
<br>
+
  /*-- creates container for all content on page --*/
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:NJAU_China&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
  font-family: Arial, Helvetica, sans-serif;
-
</td>
+
            font-weight: normal;
-
</tr>
+
            font-size: 14px;
 +
            color: #414141;
 +
            width: 960px;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
            background-color: #transparent;
 +
            margin-top: 0px;}
 +
.container {
 +
            font-size: 14px;
 +
            width:700px;
 +
            float:right;}
 +
.buttontext { color: #FFF}
 +
#my_h1{position:absolute;top:70px;left:170px;border:none;}
 +
#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
 +
#my_h2{position:absolute;top:120px;left:370px;border:none;}
 +
#my_h2 a{display:block;width:390px;height:230px;text-indent:-9999px;}
 +
#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
 +
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
 +
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
 +
#my_nav ul li:hover{background:#e3f7ff;}#my_nav ul li a{color:#00586b;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
 +
background:#c9efff;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#a1dbf0;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#c9efff;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#a1dbf0;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#c9efff;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#a1dbf0;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#c9efff;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{background:#a1dbf0;}
 +
#my_content{
 +
width:1030px;
 +
margin:0px auto;}
 +
#my_abstract{
 +
width:300px;
 +
margin:0 0 0 25px;
 +
        float:left;
 +
}
 +
#my_abstract h2{
 +
height:50px;
 +
line-height:50px;
 +
text-indent:50px;
 +
border:none;
 +
background:#999;
 +
margin:0 0 20px 0;
 +
padding:0;
 +
}
 +
#my_abstract p{
 +
text-indent:30px;
 +
height:240px;
 +
overflow-y:scroll;
 +
}
-
<tr> <td colspan="3"  height="5px"> </td></tr>
 
-
<!-- end welcome box -->
 
-
<tr>
 
-
<!--navigation menu -->
 
-
<td align="center" colspan="3">
 
-
<table  width="100%">
 
-
<tr heigth="15px"></tr>
 
-
<tr heigth="75px">
 
 +
#my_contents{
 +
width:1030px;
 +
margin:30px auto;
 +
height:800px;
 +
}
 +
#my_con{
 +
width:1000px;
 +
height:796px;
 +
float:right;
 +
position:relative;
 +
border:2px solid #999;
 +
        margin:20px 10px 0 0;
 +
}
 +
#stage_1{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:40px;
 +
border-left:2px solid #333;
 +
border-top:2px solid #333;
 +
}
 +
#stage_2{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:40px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-top:2px solid #333;
 +
}
 +
#stage_3{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:740px;
 +
left:40px;
 +
border-bottom:2px solid #333;
 +
border-left:2px solid #333;
 +
}
 +
#stage_4{
 +
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:740px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-bottom:2px solid #333;
 +
}
 +
#real_con{
 +
position:absolute;
 +
top:60px;
 +
left:60px;
 +
width:880px;
 +
height:680px;
 +
}
 +
#real_con ul{
 +
list-style:none;
 +
width:100%;
 +
height:100%;
 +
margin:0;
 +
padding:0;
 +
}
 +
#real_con ul li{
 +
width:400px;
 +
height:300px;
 +
float:left;
 +
background:#0F0;
 +
margin:26px 0 0 26px;}
 +
#real_con ul li#one_1{
 +
background:url("https://static.igem.org/mediawiki/2014/b/b4/Project_li_bg.png") no-repeat;}
 +
#real_con ul li#two_2{
 +
background:url("https://static.igem.org/mediawiki/2014/1/17/Model_li_bg.png") no-repeat;}
 +
#real_con ul li#three_3{
 +
background:url("https://static.igem.org/mediawiki/2014/6/6e/Weblab_li_bg.png") no-repeat;}
 +
#real_con ul li#four_4{
 +
background:url("https://static.igem.org/mediawiki/2014/f/f0/Practice_li_bg.png") no-repeat;}
 +
#real_con ul li a{
 +
text-decoration:none;
 +
display:block;
 +
width:100%;
 +
height:100%;
 +
}
 +
#real_con ul li a#one:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/68/Project_a_bg.png") no-repeat;}
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
#real_con ul li a#two:hover{
-
<a href="https://2014.igem.org/Team:NJAU_China"style="color:#000000">Home </a> </td>
+
background:url("https://static.igem.org/mediawiki/2014/d/dc/Model_a_bg.png") no-repeat;}
 +
#real_con ul li a#three:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/60/Weblab_a_bg.png") no-repeat;}
 +
#real_con ul li a#four:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/7/7f/Practice_a_bg.png") no-repeat;}
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:NJAU_China/Team"style="color:#000000"> Team </a> </td>
 
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
#thanks{
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=NJAU_China"style="color:#000000"> Official Team Profile </a></td>
+
height:177px;
 +
width:1030px;
 +
padding:30px 0 0 0;
 +
        clear:both;
 +
margin:30px auto 0;}
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:NJAU_China/Project"style="color:#000000"> Project</a></td>
 
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
    </style>
-
<a href="https://2014.igem.org/Team:NJAU_China/Parts"style="color:#000000"> Parts</a></td>
+
    <style>
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear {
 +
            display: none;
 +
        }
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
        /*-- hides default wiki settings --*/
-
<a href="https://2014.igem.org/Team:NJAU_China/Modeling"style="color:#000000"> Modeling</a></td>
+
    </style>
 +
<script type="text/javascript">
 +
window.onload = function(){
 +
xialashow('project_li','project');
 +
xialahidden('project_li','project');
 +
xialashow('achievement_li','achievement');
 +
xialahidden('achievement_li','achievement');
 +
xialashow('prctice_li','prctice');
 +
xialahidden('prctice_li','prctice');
 +
xialashow('ackonwledgement_li','ackonwledgement');
 +
xialahidden('ackonwledgement_li','ackonwledgement');
 +
}
 +
function xialashow(li_id,dl_id){
 +
var li_id = document.getElementById(li_id);
 +
var dl_id = document.getElementById(dl_id);
 +
li_id.onmouseover = function(){
 +
dl_id.style.display = 'block';
 +
}
 +
}
 +
function xialahidden(li_id,dl_id){
 +
var li_id = document.getElementById(li_id);
 +
var dl_id = document.getElementById(dl_id);
 +
li_id.onmouseout = function(){
 +
dl_id.style.display = 'none';
 +
}
 +
}
 +
</script>
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
</head>
-
<a href="https://2014.igem.org/Team:NJAU_China/Notebook"style="color:#000000"> Notebook</a></td>
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<!-- NAVBAR
-
<a href="https://2014.igem.org/Team:NJAU_China/Safety"style=" color:#000000"> Safety </a></td>
+
================================================== -->
 +
<body>
 +
<div id="myheader">
 +
<h1 id="my_h1"><a href="https://2014.igem.org">https://2014.igem.org</a></h1>
 +
<h2 id="my_h2"><a href="https://igem.org/Team.cgi?id=1555">https://igem.org/Team.cgi?id=1555</a></h2>
 +
    <div id="my_nav">
 +
        <ul>
 +
          <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
 +
          <li id="project_li"><a href="#">Project</a>
 +
                <dl id="project">
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Background">Background</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/System Design">System Design</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li id="achievement_li"><a href="#">Achievement</a>
 +
                <dl id="achievement">
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Lab</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Model</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Protocol/Notebooks">Protocol/Notebooks</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li id="prctice_li"><a href="#">Practice</a>
 +
                <dl id="prctice">
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Exhibition and Workshops">Exhibitions and Workshops</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire Survey">Questionnaire Survey</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Collaboration">Collaboration</a></dd>
 +
                </dl>
 +
          </li>
 +
          <li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a></li>
 +
          <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
 +
                <dl id="ackonwledgement">
 +
                      <dd><a href="https://2014.igem.org/Team:NJAU_China/Inside Campus">Inside Campus&Arrtibutions</a></dd>
 +
                    <dd><a href="https://2014.igem.org/Team:NJAU_China/Outside Campus">Outside Campus</a></dd>
 +
                </dl>
 +
          </li>
 +
      </ul>
 +
    </div>
 +
</div>
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
-
<a href="https://2014.igem.org/Team:NJAU_China/Attributions"style="color:#000000"> Attributions </a></td>
 
 +
<!---------导航结束------------>
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
 
-
</tr>
 
-
</table>
 
-
<!--end navigation menu -->
+
<!-- Carousel
-
</tr>
+
    ================================================== -->
 +
<div id="my_content">
 +
<div id="my_abstract">
 +
    <h2>Abstract</h2>
 +
    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
 +
</div>
 +
<div class="container">
 +
    <div id="myCarousel" class="carousel slide" data-ride="carousel">
 +
        <!-- Indicators -->
 +
        <ol class="carousel-indicators">
 +
            <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
 +
            <li data-target="#myCarousel" data-slide-to="1"></li>
 +
            <li data-target="#myCarousel" data-slide-to="2"></li>
 +
        </ol>
 +
        <div class="carousel-inner" role="listbox">
 +
            <div class="item active text-center"><img src="https://static.igem.org/mediawiki/2014/d/d3/Njau_slide1.jpg"
 +
                                                      alt="First slide" class="text-center"></div>
 +
            <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/3/38/Face.jpg"
 +
                                              alt="Second slide"></div>
 +
            <div class="item text-center"><img src="https://static.igem.org/mediawiki/2014/9/94/Face2.jpg"
 +
                                              alt="Third slide"></div>
 +
        </div>
 +
        <a class="left carousel-control" href="#myCarousel" role="button" data-slide="prev"> <span class="sr-only">Previous</span>
 +
        </a> <a class="right carousel-control" href="#myCarousel" role="button" data-slide="next"> <span
 +
            class="sr-only">Next</span> </a></div>
 +
    <!-- /.carousel -->
 +
</div>
 +
</div>
 +
<div id="my_contents">
 +
<div id="my_con">
 +
    <div id="stage_1"></div>
 +
        <div id="stage_2"></div>
 +
        <div id="stage_3"></div>
 +
        <div id="stage_4"></div>
 +
        <div id="real_con">
 +
        <ul>
 +
            <li id="one_1"><a href="https://2014.igem.org/Team:NJAU_China/Background" id="one"></a></li>
 +
                <li id="two_2"><a href="https://2014.igem.org/Team:NJAU_China/Modeling" id="two"></a></li>
 +
                <li id="three_3"><a href="https://2014.igem.org/Team:NJAU_China/Wet_Labs" id="three"></a></li>
 +
                <li id="four_4"><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire_Survey" id="four"></a></li>
 +
            </ul>
 +
        </div>
 +
    </div>
 +
</div>
-
</tr>
+
<div id="thanks">
-
+
<img src="https://static.igem.org/mediawiki/2014/2/26/Njau_bg.png" alt="njau"><img src="https://static.igem.org/mediawiki/2014/a/ae/Shengke_bg.png" alt="shengke">
-
 
+
</div>
-
 
+
</body>
-
 
+
</html>
-
 
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--requirements section -->
+
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
-
<tr>
+
-
<td width="45%"  valign="top">
+
-
 
+
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
+
-
 
+
-
<!-- Links to other team pages -->
+
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China">Home</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a> </li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=NJAU_China">Official Team Profile</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Project">Project</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Parts">Parts</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Modeling</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Notebook">Notebook</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Safety">Safety</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:NJAU_China/Attributions">Attributions</a> </li>
+
-
 
+
-
</ul>
+
-
 
+
-
</td>
+
-
 
+
-
<td > </td>
+
-
<td width="45%">
+
-
 
+
-
<p>There are a few wiki requirements teams must follow:</p>
+
-
<ul>
+
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
+
-
<li>All pages must be created under the team’s name space.</li>
+
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
+
-
<li>Do not use flash in wiki code. </li>
+
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
+
-
</ul>
+
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
 
+
-
<tr> <td colspan="3" height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
+
-
 
+
-
 
+
-
<!--tips  -->
+
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
-
 
+
-
<tr>
+
-
<td width="45%" valign="top">
+
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
 
+
-
<ul>
+
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
-
</ul>
+
-
 
+
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
-
</td>
+
-
 
+
-
<td> </td>
+
-
<td width="45%">
+
-
 
+
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
-
 
+
-
<ul>
+
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
-
<li>Have lots of fun! </li>
+
-
</ul>
+
-
</br>
+
-
</td>
+
-
</tr>
+
-
</table>
+

Latest revision as of 17:03, 19 November 2014

Home

Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

Retrieved from "http://2014.igem.org/Team:NJAU_China"