Team:NJAU China

From 2014.igem.org

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<html lang="en">
<html lang="en">
<head>
<head>
-
<meta charset="utf-8">
+
    <meta charset="utf-8">
-
<meta http-equiv="X-UA-Compatible" content="IE=edge">
+
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
-
<meta name="viewport" content="width=device-width, initial-scale=1">
+
    <meta name="viewport" content="width=device-width, initial-scale=1">
-
<meta name="description" content="">
+
    <meta name="description" content="">
-
<meta name="author" content="">
+
    <meta name="author" content="">
-
<link rel="icon" href="../../favicon.ico">
+
    <link rel="icon" href="../../favicon.ico">
-
<title>Carousel Template for Bootstrap</title>
+
    <title>Home</title>
-
<link rel="stylesheet" href="http://cdn.bootcss.com/bootstrap/3.2.0/css/bootstrap.min.css">
+
    <script src="https://2014.igem.org/Team:NJAU_China/jquery.min.js?action=raw"></script>
-
<script src="http://cdn.bootcss.com/jquery/1.11.1/jquery.min.js"></script>
+
    <script src="https://2014.igem.org/Team:NJAU_China/bootstrap.min.js?action=raw"></script>
-
<script src="http://cdn.bootcss.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
+
-
<!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
+
    <link rel="stylesheet" type="text/css"
-
<!--[if lt IE 9]>
+
          href="https://2014.igem.org/Team:NJAU_China/bootstrap.min.css?action=raw&amp;ctype=text/css">
-
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+
    <link rel="stylesheet" type="text/css"
-
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+
          href="https://2014.igem.org/Team:NJAU_China/bootstrap-theme.min.css?action=raw&amp;ctype=text/css">
 +
    <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
 +
    <!--[if lt IE 9]>
 +
    <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
 +
    <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
     <![endif]-->
     <![endif]-->
-
<!-- Custom styles for this template -->
+
    <!-- Custom styles for this template -->
-
<link href="carousel.css" rel="stylesheet">
+
    <link href="carousel.css" rel="stylesheet">
-
<style type="text/css">
+
    <style type="text/css">
-
body {
+
        body {
-
padding-bottom: 40px;
+
            padding-bottom: 40px;
-
background: #333333;
+
            background:#a2dcf3;
-
color: #5a5a5a;
+
            color: #5a5a5a;
-
font-size: 12px;
+
            font-size: 14px;
 +
            font-family:Andalus;
 +
        }
 +
        #myheader{
 +
            width:1366px;
 +
    height:500px;
 +
    margin:0 auto;
 +
    position:relative;
 +
    font-family:Arial, Helvetica, sans-serif;
 +
            background:#a2dcf3 url("https://static.igem.org/mediawiki/2014/3/34/Header_bg.png");
 +
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 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
 +
            display: none;
 +
        }
 +
 
 +
        /*-- hides default wiki settings --*/
 +
        .firstHeading {
 +
            width: 975px;
 +
            margin: 0px auto;
 +
            padding-top: 100px;
 +
            margin-bottom: 20px;
 +
            font-family: Georgia, Times, "Andalus", serif;
 +
        }
 +
 
 +
        h1, h2, h3, h4, h5 {font-family: Georgia, Times, "Andalus", serif;}
 +
        #top-section {
 +
            /*-- styling for default menu bar (edit, page, history, etc.) --*/ background-color: #383838;border: 0 none;height: 14px;z-index: 100;top: 0;position: fixed; width: 975px;left: 50%;margin-left: -487px;}
 +
#top-section-bar {
 +
            /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
 +
  background-color: #383838;
 +
  height: 14px;
 +
  display: block;
 +
  z-index: 10;position: fixed; width: 100%; top: 0;}
 +
  body { background-color: #fff;
 +
  }
 +
#globalWrapper, #content {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  border: 0px;
 +
  background-color: transparent;
 +
  margin: 0px;
 +
  padding: 0px;
 +
  }
 +
 
 +
  html, body, .wrapper {
 +
  /*-- changes default wiki settings --*/
 +
  width: 100%;
 +
  height: 100%;
 +
  background-color: transparent;
 +
  } #container {
 +
  /*-- creates container for all content on page --*/
 +
  font-family: Arial, Helvetica, sans-serif;
 +
            font-weight: normal;
 +
            font-size: 14px;
 +
            color: #414141;
 +
            width: 960px;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
            background-color: #transparent;
 +
            margin-top: 0px;}
 +
.container {
 +
            font-size: 14px;
 +
            width:700px;
 +
            float:right;}
 +
.buttontext { color: #FFF}
 +
#my_h1{position:absolute;top:70px;left:170px;border:none;}
 +
#my_h1 a{display:block;width:190px;height:130px;text-indent:-9999px;}
 +
#my_h2{position:absolute;top:120px;left:370px;border:none;}
 +
#my_h2 a{display:block;width:390px;height:230px;text-indent:-9999px;}
 +
#my_nav{position:absolute;top:402px;left:200px;width:990px;height:50px;}
 +
#my_nav ul{width:100%;margin:0;padding:0;list-style:none;font-size:24px;}
 +
#my_nav ul li{display::inline-block;padding:9px 30px;float:left;list-style:none;position:relative;}
 +
#my_nav ul li:hover{background:#e3f7ff;}#my_nav ul li a{color:#00586b;text-decoration:none;}#project{display:none;position:absolute;z-index:100;top:47px;left:0px;background:#CF6;width:100px;}#project dd{width:135px;font-size:16px;
 +
background:#c9efff;height:40px;margin:0;}#project dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#project dd a:hover{background:#a1dbf0;}#achievement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#achievement dd{width:198px;font-size:16px;background:#c9efff;height:40px;margin:0;}#achievement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#achievement dd a:hover{background:#a1dbf0;}#prctice{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#prctice dd{width:300px;font-size:16px;background:#c9efff;height:40px;margin:0;}#prctice dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#prctice dd a:hover{background:#a1dbf0;}#ackonwledgement{display:none;position:absolute;top:47px;z-index:100;left:0px;background:#CF6;width:100px;}#ackonwledgement dd{width:257px;font-size:16px;background:#c9efff;height:40px;margin:0;}#ackonwledgement dd a{width:100%;height:40px;display:block;text-decoration:none;text-align:center;line-height:40px;}#ackonwledgement dd a:hover{background:#a1dbf0;}
 +
#my_content{
 +
width:1030px;
 +
margin:0px auto;}
 +
#my_abstract{
 +
width:300px;
 +
margin:0 0 0 25px;
 +
        float:left;
 +
}
 +
#my_abstract h2{
 +
height:50px;
 +
line-height:50px;
 +
text-indent:50px;
 +
border:none;
 +
background:#999;
 +
margin:0 0 20px 0;
 +
padding:0;
}
}
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {
+
#my_abstract p{
-
display: none;
+
text-indent:30px;
-
} /*-- hides default wiki settings --*/
+
height:240px;
-
.firstHeading {
+
overflow-y:scroll;
-
width: 975px;
+
-
margin: 0px auto;
+
-
padding-top: 100px;
+
-
margin-bottom: 20px;
+
-
font-family: Georgia, Times, "Times New Roman", serif;
+
}
}
-
h1, h2, h3, h4, h5 {
+
 
-
font-family: Georgia, Times, "Times New Roman", serif;
+
 
 +
 
 +
 
 +
#my_contents{
 +
width:1030px;
 +
margin:30px auto;
 +
height:800px;
}
}
-
#top-section { /*-- styling for default menu bar (edit, page, history, etc.) --*/
+
#my_con{
-
background-color: #383838;
+
width:1000px;
-
border: 0 none;
+
height:796px;
-
height: 14px;
+
float:right;
-
z-index: 100;
+
position:relative;
-
top: 0;
+
border:2px solid #999;
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position: fixed;
+
        margin:20px 10px 0 0;
-
width: 975px;
+
-
left: 50%;
+
-
margin-left: -487px;
+
}
}
-
#top-section-bar { /*-- styling full width bar which hides behind default menu bar (edit, page, history, etc.) --*/
+
#stage_1{
-
background-color: #383838;
+
width:20px;
-
height: 14px;
+
height:20px;
-
display: block;
+
position:absolute;
-
z-index: 10;
+
top:40px;
-
position: fixed;
+
left:40px;
-
width: 100%;
+
border-left:2px solid #333;
-
top: 0;
+
border-top:2px solid #333;
}
}
-
#menubar a:link, #menubar a:active, #menubar a:visited, #menubar a:hover, #menubar:hover { /*-- styling for default menu bar links (edit, page, history, etc.) --*/
+
#stage_2{
-
color: #727272;
+
width:20px;
-
text-decoration: none;
+
height:20px;
-
background-color: transparent;
+
position:absolute;
 +
top:40px;
 +
left:940px;
 +
border-right:2px solid #333;
 +
border-top:2px solid #333;
}
}
-
body {
+
#stage_3{
-
background-color: #fff;
+
width:20px;
 +
height:20px;
 +
position:absolute;
 +
top:740px;
 +
left:40px;
 +
border-bottom:2px solid #333;
 +
border-left:2px solid #333;
}
}
-
#globalWrapper, #content { /*-- changes default wiki settings --*/
+
#stage_4{
-
width: 100%;
+
width:20px;
-
height: 100%;
+
height:20px;
-
border: 0px;
+
position:absolute;
-
background-color: transparent;
+
top:740px;
-
margin: 0px;
+
left:940px;
-
padding: 0px;
+
border-right:2px solid #333;
 +
border-bottom:2px solid #333;
}
}
-
html, body, .wrapper { /*-- changes default wiki settings --*/
+
#real_con{
-
width: 100%;
+
position:absolute;
-
height: 100%;
+
top:60px;
-
background-color: transparent;
+
left:60px;
 +
width:880px;
 +
height:680px;
}
}
-
#container { /*-- creates container for all content on page --*/
+
#real_con ul{
-
font-family: Arial, Helvetica, sans-serif;
+
list-style:none;
-
font-weight: normal;
+
width:100%;
-
font-size: 14px;
+
height:100%;
-
color: #414141;
+
margin:0;
-
width: 960px;
+
padding:0;
-
margin-left: auto;
+
-
margin-right: auto;
+
-
background-color: #transparent;
+
-
margin-top: 0px;
+
}
}
-
.sidemenu, .sidemenu li {
+
#real_con ul li{
-
list-style-type: none;
+
width:400px;
-
list-style-image: none;
+
height:300px;
-
font-family: verdana;
+
float:left;
-
text-decoration: none;
+
background:#0F0;
-
color:#000;
+
margin:26px 0 0 26px;}
-
font-size: 14px;
+
#real_con ul li#one_1{
-
}
+
background:url("https://static.igem.org/mediawiki/2014/b/b4/Project_li_bg.png") no-repeat;}
-
.sidemenu li {
+
#real_con ul li#two_2{
-
display: block;
+
background:url("https://static.igem.org/mediawiki/2014/1/17/Model_li_bg.png") no-repeat;}
-
}
+
#real_con ul li#three_3{
-
.sidemenu a {
+
background:url("https://static.igem.org/mediawiki/2014/6/6e/Weblab_li_bg.png") no-repeat;}
 +
#real_con ul li#four_4{
 +
background:url("https://static.igem.org/mediawiki/2014/f/f0/Practice_li_bg.png") no-repeat;}
 +
#real_con ul li a{
text-decoration:none;
text-decoration:none;
-
color: #000;
+
display:block;
-
display: block;
+
width:100%;
-
width: 180px;
+
height:100%;
-
height: 100%;
+
-
padding: 3px 5px;
+
-
transition: .25s ease-in-out;
+
-
-moz-transition: .25s ease-in-out;
+
-
-webkit-transition: .25s ease-in-out;
+
}
}
-
.sidemenu a.greyout {
+
#real_con ul li a#one:hover{
-
color: #909090;
+
background:url("https://static.igem.org/mediawiki/2014/6/68/Project_a_bg.png") no-repeat;}
 +
 
 +
#real_con ul li a#two:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/d/dc/Model_a_bg.png") no-repeat;}
 +
#real_con ul li a#three:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/6/60/Weblab_a_bg.png") no-repeat;}
 +
#real_con ul li a#four:hover{
 +
background:url("https://static.igem.org/mediawiki/2014/7/7f/Practice_a_bg.png") no-repeat;}
 +
 
 +
 
 +
#thanks{
 +
height:177px;
 +
width:1030px;
 +
padding:30px 0 0 0;
 +
        clear:both;
 +
margin:30px auto 0;}
 +
 
 +
 
 +
    </style>
 +
    <style>
 +
        #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear {
 +
            display: none;
 +
        }
 +
 
 +
        /*-- hides default wiki settings --*/
 +
    </style>
 +
<script type="text/javascript">
 +
window.onload = function(){
 +
xialashow('project_li','project');
 +
xialahidden('project_li','project');
 +
xialashow('achievement_li','achievement');
 +
xialahidden('achievement_li','achievement');
 +
xialashow('prctice_li','prctice');
 +
xialahidden('prctice_li','prctice');
 +
xialashow('ackonwledgement_li','ackonwledgement');
 +
xialahidden('ackonwledgement_li','ackonwledgement');
}
}
-
.sidemenu a:hover {
+
function xialashow(li_id,dl_id){
-
color:#666;
+
var li_id = document.getElementById(li_id);
-
background-color: #d9f5aa;
+
var dl_id = document.getElementById(dl_id);
 +
li_id.onmouseover = function(){
 +
dl_id.style.display = 'block';
 +
}
}
}
-
.sub-sidemenu {
+
function xialahidden(li_id,dl_id){
-
font-size: 10px;
+
var li_id = document.getElementById(li_id);
-
margin-bottom: 20px;
+
var dl_id = document.getElementById(dl_id);
-
color: #666;
+
li_id.onmouseout = function(){
-
display: none;
+
dl_id.style.display = 'none';
 +
}
}
}
-
.sub-sidemenu a {
+
</script>
-
color: #666;
+
 
-
}
+
-
div.underconst {
+
-
padding:10px;
+
-
border-radius:5px;
+
-
background-color:pink;
+
-
text-align:center;
+
-
}
+
-
a.anchor {
+
-
display: block;
+
-
position: relative;
+
-
top: -80px;
+
-
visibility: hidden;
+
-
}
+
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.contentpara {
+
-
margin-bottom: 30px;
+
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}
+
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span.email {
+
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font-family: monospace;
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+
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fieldset {
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border: 1px solid #337f53;
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+
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.greyout {
+
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color: #A0A0A0;
+
-
}
+
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.highlightme {
+
-
background-color: #FFFF00;
+
-
}
+
-
#alertContainer {
+
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border: 2px solid red;
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margin-bottom: 10px;
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width: 936px;
+
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+
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#annContainer {
+
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margin-left: 8px;
+
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}
+
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#newsContainer {
+
-
border: 1px solid #ccc;
+
-
}
+
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.newsTitle {
+
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display: block;
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color: #414141;
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font-size: 25px;
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font-family: Georgia, Times, "Times New Roman", serif;
+
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padding: 10px 15px 5px 10px;
+
-
border-bottom: 1px solid #ccc;
+
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margin-bottom: 0px;
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}
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.newsItem {
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border-bottom: 1px solid #ccc;
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display: block;
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padding: 5px 15px 0px 10px;
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margin-bottom: 0px;
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+
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.newsItem h3 {
+
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width: auto;
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display: inline;
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font-size: 14px;
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font-family: Arial, Helvetica, sans-serif;
+
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padding: 0px;
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margin: 0px;
+
-
}
+
-
.newsItem img {
+
-
float: right;
+
-
clear: right;
+
-
width: 80px;
+
-
padding-left: 15px;
+
-
display: inline-block;
+
-
}
+
-
.newsItem .newsDate {
+
-
font-style: italic;
+
-
font-size: 14px;
+
-
display: inline-block;
+
-
color: #999;
+
-
float: right;
+
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padding: 0px 0px 10px 15px;
+
-
}
+
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.newsItem p, .newsItem ul, .newsItem li {
+
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margin-left: 20px;
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font-size: 12px;
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line-height: 1.2;
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.newsItem p {
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margin-top: 10px;
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+
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vertical-align: middle;
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-
}
+
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.annItem .annCentered {
+
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display: table-cell;
+
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vertical-align: middle;
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height: 160px;
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+
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.annCentered h3 {
+
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padding: 0px;
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display: block;
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margin-left: auto;
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.annItem ul, .annItem li {
+
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margin: 0px;
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+
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.annItem li {
+
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margin: 5px
+
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+
-
.container {
+
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font-size:14px
+
-
}
+
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.buttontext {
+
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color:#FFF
+
-
}
+
-
</style>
+
-
<style>
+
-
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
+
-
</style>
+
</head>
</head>
 +
<!-- NAVBAR
<!-- NAVBAR
================================================== -->
================================================== -->
<body>
<body>
-
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    <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
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Latest revision as of 17:03, 19 November 2014

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Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

Retrieved from "http://2014.igem.org/Team:NJAU_China"