Team:NJAU China

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<body>
<body>
<div id="myheader">
<div id="myheader">
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<h1 id="my_h1"><a href="#">IGEM</a></h1>
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<h1 id="my_h1"><a href="https://2014.igem.org">https://2014.igem.org</a></h1>
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<h2 id="my_h2"><a href="https://igem.org/Team.cgi?id=1555">https://igem.org/Team.cgi?id=1555</a></h2>
     <div id="my_nav">
     <div id="my_nav">
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         <ul style="list-style-type:none;">
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         <ul>
           <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
           <li><a href="https://2014.igem.org/Team:NJAU_China">Home</a></li>
           <li id="project_li"><a href="#">Project</a>
           <li id="project_li"><a href="#">Project</a>
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           <li id="achievement_li"><a href="#">Achievement</a>
           <li id="achievement_li"><a href="#">Achievement</a>
                 <dl id="achievement">
                 <dl id="achievement">
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Labs</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Wet Labs">Wet Lab</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Modeling</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Modeling">Model</a></dd>
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                     <dd><a href="#">Protocol/Notebooks</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Protocol/Notebooks">Protocol/Notebooks</a></dd>
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Biobricks">Biobricks</a></dd>
                 </dl>
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           <li id="prctice_li"><a href="#">Practice</a>
           <li id="prctice_li"><a href="#">Practice</a>
                 <dl id="prctice">
                 <dl id="prctice">
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                     <dd><a href="#">iGEM and Synthetic Biology Exhibition</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Exhibition and Workshops">Exhibitions and Workshops</a></dd>
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                     <dd><a href="#">Questionnaire Survey</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Questionnaire Survey">Questionnaire Survey</a></dd>
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                     <dd><a href="#">Collaboration</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Collaboration">Collaboration</a></dd>
                 </dl>
                 </dl>
           </li>
           </li>
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           <li><a href="#">Team</a></li>
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           <li><a href="https://2014.igem.org/Team:NJAU_China/Team">Team</a></li>
           <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
           <li id="ackonwledgement_li"><a href="#">Acknowledgement</a>
                 <dl id="ackonwledgement">
                 <dl id="ackonwledgement">
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                    <dd><a href="#">Genewiz Corporation</a></dd>
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                      <dd><a href="https://2014.igem.org/Team:NJAU_China/Inside Campus">Inside Campus&Arrtibutions</a></dd>
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                     <dd><a href="#">Chinese Academy of Sciences</a></dd>
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                     <dd><a href="https://2014.igem.org/Team:NJAU_China/Outside Campus">Outside Campus</a></dd>
                 </dl>
                 </dl>
           </li>
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<div id="my_abstract">
<div id="my_abstract">
     <h2>Abstract</h2>
     <h2>Abstract</h2>
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     <p>As copper is an essential element for industry, low concentration of it can do harm to many creatures. Whereas, the Escherichia coli has high tolerance for copper through specific systems. There are copA promoter、marO（operator of mar locus）、cueO promoter existed in E.Coli, both are directly or indirectly regulated by copper ions. The most sensitive and effective would be chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. Glutathione can be synthesized by GshF, an enzyme derived from Streptococcus thermophilus, which can unifies both necessary reactions in one platform. In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. Finally, an automatically and efficiently copper-preventing guard are hoped to built. </p>
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     <p>As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, <i>Escherichia coli</i> has high tolerance for copper through specific systems. There are <i>cop</i>A promoter,<i>mar</i>O（operator of mar locus） and <i>cue</i>O promoter existing in <i>E.Coli</i>, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (<i>cop</i>A)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by <i>gsh</i>F, an coding sequence derived from <i>Streptococcus thermophilus</i>, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of <i>cop</i>A. With all these design, an automatically and efficiently copper-preventing guard have been created successfully. </p>
</div>
</div>
<div class="container">
<div class="container">
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                                               alt="Third slide"></div>
                                               alt="Third slide"></div>
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          <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/protocol#Basic">Basic Experiments of Molecular Biology</a></li>
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        <div id="stage_3"></div>
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          <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/protocol#Observation">Observation and Measurement of GFP Expression</a></li>
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        <div id="stage_4"></div>
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          <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/protocol#Drawing">Drawing Growth Curve</a></li>
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        <div id="real_con">
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          <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/protocol#HPLC">HPLC</a></li>
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        <ul>
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          <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/protocol#Verification">Verification of Motility from Phenotype</a></li>
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Latest revision as of 17:03, 19 November 2014

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Abstract

As an common pollutant source of industry, low concentration of copper ions can do harm to many creatures. Whereas, Escherichia coli has high tolerance for copper through specific systems. There are copA promoter,marO（operator of mar locus） and cueO promoter existing in E.Coli, all of which are directly or indirectly regulated by copper ions. The most sensitive and effective one (copA)was chosen to trigger the sequential reactions. Glutathione is an intracellular redox-active tripeptide thiol which can effectively detoxify the metal ions by chelation. It can be synthesized by gshF, an coding sequence derived from Streptococcus thermophilus, which can unify both necessary reactions in one platform.In addition, we also utilize sgRNA-dCas9 system to enhance the efficiency of chelate cupric and cuprous by repressing the expression of copA. With all these design, an automatically and efficiently copper-preventing guard have been created successfully.

njaushengke

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