Team:Valencia UPV/VUPV Parts

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<div align="center"><span class="coda"><roja>P</roja>arts</span> </div><br/>
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<p>Our team sent 7 BioBrick Parts to the <a class="normal-link-page" href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2014&group=Valencia_UPV">Registry</a>.</p><br/>
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<p>Our team sent 7 BioBrick parts to the Registry.</p><br/>
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<p>Four of them could be sent thanks to the collaboration from the UEA team, who very kindly provided us with their MoFlippers. These were the parts coding sequences for AtrΔ11, HarFAR and EaDAcT and the TA29 promoter.</p><br/>
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<div align="center"><groupparts>iGEM014 Valencia_UPV</groupparts></div>
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<p>We designed other vector which can be used to assemble two transcriptional units in alpha. It has two BsmBI sites which leave overhangs that match with GoldenBraid 2.0 grammar. We call them "omega undercover" as it works in a similar way of the GoldenBraid 2.0 omega vector but with a pSB1A3 backbone.</p><br/>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/4/45/VUPVomega_undercover.jpg" alt="omega_undercover"></img></div><br/>
 
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554000">BBa_K1554000</a> – Ta29 Promoter</b></p>
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<p>The Ta29 promoter is anther-specific. Anthers are the male organs of the flower, and their function is to produce pollen. A cytotoxic gene (barnase, ipt, RIP...) is usually used under the control of this specific promoter leading to a plant unable to produce pollen, inducing male-sterility. <a href=https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosafety> See Biosafety</p><br/>
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<p>Using the omega undercover plasmid two biosafety constructions we constructed the two biosafety devices which consisted on an identity preservation module, expressing either yellow or blue chromoprotein, and a male sterility module.</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554001">BBa_K1554001</a> – AtrΔ11</b></p>
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<p>The AtrΔ11 protein is a delta-11-desaturase from Amyelois transitella that introduces an unsaturation between C11 and C12 in long-chain fatty acids. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana.
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Acyl-CoA + Reduced acceptor + O2 = Delta11-acyl-CoA + Acceptor + 2 H2O
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This part was used in the Insect sexual pheromone production pathway. <a href=https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis> See Biosynthesis</p><br/>
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<div align="center"><img src="https://static.igem.org/mediawiki/2014/e/e2/VUPV_biosafety_constr_omega_undercover.jpg" alt="omega_undercover2"></img></div><br/><br/><br/><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554002">BBa_K1554002</a> – HarFAR</b></p>
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<p>The HarFAR protein is a fatty acid reductase that catalyses the conversion of a long-chain fatty acid carboxyl group to an alcohol group. The DNA sequence was obtained from Helicoverpa armigera HarFAR-3 protein, including an Endoplasmic Reticulum retention signal (KKYR) in the C-terminal end. In addition, codon usage optimization was performed for Nicotiana benthamiana.
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This part was used in the Insect sexual pheromone production pathway. <a href=https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis> See Biosynthesis</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554003">BBa_K1554003</a> – EaDAcT</b></p>
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<p>The EaDAcT protein is a diacylglycerol acetyltransferase coming from Euonymus alatus. This enzyme can transform alcohol gropus from fatty acid to aldehyde gropus. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana.
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This part was used in the Insect sexual pheromone production pathway. <a href=https://2014.igem.org/Team:Valencia_UPV/Project/modules/biosynthesis> See Biosynthesis </a></p><br/>
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<div align="center"><groupparts>iGEM014 Valencia_UPV</groupparts></div>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554004">BBa_K1554004</a> – Yellow biosafety module for plants</b></p>
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<p>This biosafety device for plants consists of two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consists of a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants. See Results: Constructs-Biosafety</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554005">BBa_K1554005</a> - Blue biosafety module for plants</b></p>
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<p>This biosafety device is composed of the same parts as the previously explained one, BBa_K1554004. This device uses AmilCP as chromoprotein instead of AmilGFP. See Results: Constructs-Biosafety</p><br/>
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<p> <b><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1554006">BBa_K1554006</a> - Omega Undercover</b></p>
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<p>The Omega undercover vector allows the conversión of GoldenBraid standard parts to BioBrick’s. <a href=https://2014.igem.org/Team:Valencia_UPV/Project/modules/methodology/parts_construction> See Methodology: Parts construction </a></p><br/>
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Latest revision as of 18:15, 13 November 2014

Achievements > Parts


Parts

Our team sent 7 BioBrick Parts to the Registry.


<groupparts>iGEM014 Valencia_UPV</groupparts>


BBa_K1554000 – Ta29 Promoter

The Ta29 promoter is anther-specific. Anthers are the male organs of the flower, and their function is to produce pollen. A cytotoxic gene (barnase, ipt, RIP...) is usually used under the control of this specific promoter leading to a plant unable to produce pollen, inducing male-sterility. See Biosafety


BBa_K1554001 – AtrΔ11

The AtrΔ11 protein is a delta-11-desaturase from Amyelois transitella that introduces an unsaturation between C11 and C12 in long-chain fatty acids. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana. Acyl-CoA + Reduced acceptor + O2 = Delta11-acyl-CoA + Acceptor + 2 H2O This part was used in the Insect sexual pheromone production pathway. See Biosynthesis


BBa_K1554002 – HarFAR

The HarFAR protein is a fatty acid reductase that catalyses the conversion of a long-chain fatty acid carboxyl group to an alcohol group. The DNA sequence was obtained from Helicoverpa armigera HarFAR-3 protein, including an Endoplasmic Reticulum retention signal (KKYR) in the C-terminal end. In addition, codon usage optimization was performed for Nicotiana benthamiana. This part was used in the Insect sexual pheromone production pathway. See Biosynthesis


BBa_K1554003 – EaDAcT

The EaDAcT protein is a diacylglycerol acetyltransferase coming from Euonymus alatus. This enzyme can transform alcohol gropus from fatty acid to aldehyde gropus. This DNA sequence underwent codon usage optimization for Nicotiana benthamiana. This part was used in the Insect sexual pheromone production pathway. See Biosynthesis


BBa_K1554004 – Yellow biosafety module for plants

This biosafety device for plants consists of two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consists of a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants. See Results: Constructs-Biosafety


BBa_K1554005 - Blue biosafety module for plants

This biosafety device is composed of the same parts as the previously explained one, BBa_K1554004. This device uses AmilCP as chromoprotein instead of AmilGFP. See Results: Constructs-Biosafety


BBa_K1554006 - Omega Undercover

The Omega undercover vector allows the conversión of GoldenBraid standard parts to BioBrick’s. See Methodology: Parts construction







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