Shipped Parts

We are happy to announce that we have successfully created four BioBricks over the course of this summer! Among them is one intein BioBrick, the Ssp GyrB Split Intein which will surely be useful in many future projects.

• K1483000: α-N-Acetylgalactosamindase

  The α-N-acetylgalactosamindase (NAGA) is an enzyme from Elizabethkingia meningoseptica capable of cleaving off N-acetlygalactosamine from A-group blood antigens (Figure 1).



Figure 1: Schematic illustration of NAGA's enzymatic cleavage of A-group blood antigens while erythrocytes pass through a NAGA-coated clumn.

• K1483001: Endo-β-Galactosidase

  The endo-β-galactosidase - also called EABase - is an enzyme from Clostridium perfringens which can cleave off trisaccharides from the A- and B-group blood antigens which leaves behind a precursor of the H-antigen (Figure 2).



Figure 2: Schematic illustration of EABase's enzymatic cleavage of A- and B-group blood antigens while erythrocytes pass through a EABase-coated column. EABase leaves behind Oh antigens.

• K1483002: α-Galactosidase

  This Bacteroides fragilis enzyme α-galactosidase (aGAL) cleaves off B-group blood antigens from erythrocytes thereby creating H-antigens (Figure 3).



Figure 3: Schematic illustration of aGAL's enzymatic cleavage of B-group blood antigens while erythrocytes pass through a aGAL-coated column.

• K1483003: Ssp GyrB Split Intein

  This protein naturally occurs in Synechocystis sp. PCC6803. The gyr B split intein is a bacterial intein with a 6 aminoacid long C-intein and a 150 aminoacid long N-intein and can be used e.g. to fuse two proteins (Figure 4).



Figure 4: Schematic illustration of the interaction of N- and C-intein resulting in a covalent binding between the N- and C-intein-carrying substances (e.g. proteins).