Team:SUSTC-Shenzhen/Project/Plasmid

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Here are the full list of plasmids we've used and constructed and their names and maps have universality.
Here are the full list of plasmids we've used and constructed and their names and maps have universality.
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=gRNA-mCherry-NLS-UAS plasmids=
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{{SUSTC-Image|wiki/images/8/87/SUSTC-Shenzhen-gRNAforEGFP.png}}
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=Plasmids from Germany=
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pSW55-GD5 (partial)
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Uherek et al. 1998
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Brief introduction:
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pSW55-GD5 encodes the chimeric fusion protein GD5 working as non-viral vector that can transfer specific target gene into specific target cells.
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Contains:
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partial sequence (coding sequence)
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beginning with NdeI site (CATATG)
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vector backbone (not included):
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pFLAG-1, ompA signal peptide removed
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nt 4-1854: coding sequence GD5 fusion protein
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nt 7-30 FLAG peptide
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nt 37-54: 6 x His tag
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nt 55-492: GAL4 residues 2 to 147 (DNA binding domain)
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nt 505-1071 DT-B translocation domain
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nt 1093-1815: scFv(FRP5) (anti-ErbB2)
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[[File:SUSTC-Shenzhen-pSW55-GD5(coding_sequence).jpg|thumb|Fig.1 GD5]]
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Click the linking at the following for detailed information
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https://2014.igem.org/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify
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lick the linking at the following for summarized information
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https://2014.igem.org/Team:SUSTC-Shenzhen/Project/A-B_toxin#Construction
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pWF47-TEG (partial)
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Fominaya et al. 1998
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(corrected 2014-07-31)
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Brief introduction:
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pWF47-TEG encodes the chimeric fusion protein TEG working as non-viral vector that can transfer specific target gene into specific target cells.
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partial sequence (coding sequence)
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beginning with second half of NdeI site (catATG)
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vector backbone (not included): pFLAG-1
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nt 1-1089: coding sequence TEG fusion protein
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nt 1-63: ompA signal peptide (pFLAG-1)
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nt 64-87: FLAG peptide (pFLAG-1)
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nt 100-249: hTGFalpha residues 40 to 89 (mature form)
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nt 259-276: 6 x His tag
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nt 280-615: ETA domain II
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nt 628-1065: GAL4 residues 2 to 147 (DNA binding domain)
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nt 1078-1089: KDEL ER retention sequence
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[[File:SUSTC-Shenzhen-PWF47-TEG(coding_sequence).jpg|thumb|Fig.2 TEG]]
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Click the linking at the following for detailed information
 +
https://2014.igem.org/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify
 +
lick the linking at the following for summarized information
 +
https://2014.igem.org/Team:SUSTC-Shenzhen/Project/A-B_toxin#Construction
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=Target-sequence=
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[https://2014.igem.org/Team:SUSTC-Shenzhen/Project/gRNA-binding-target-sequence_Plasmids_Design_and_Construction '''Target-sequence_for_HIV''']
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[[File:Target-sequence_for_HIV_Map.png]]
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[https://2014.igem.org/Team:SUSTC-Shenzhen/Project/gRNA-binding-target-sequence_Plasmids_Design_and_Construction '''Target-sequence_for_HBV2''']
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[[File:Target-sequence_for_HBV2_Map.png]]
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[https://2014.igem.org/Team:SUSTC-Shenzhen/Project/gRNA-binding-target-sequence_Plasmids_Design_and_Construction '''Target-sequence_for_HBV1-2''']
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[[File:Target-sequence_for_HBV1-2_Map.png]]
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Latest revision as of 03:55, 18 October 2014

Team SUSTC-Shenzhen

Plasmid

Full list of plasmid we used and constructed

Contents


Here are the full list of plasmids we've used and constructed and their names and maps have universality.

gRNA-mCherry-NLS-UAS plasmids

{{{2}}}

Plasmids from Germany

pSW55-GD5 (partial) Uherek et al. 1998 Brief introduction: pSW55-GD5 encodes the chimeric fusion protein GD5 working as non-viral vector that can transfer specific target gene into specific target cells.

Contains: partial sequence (coding sequence) beginning with NdeI site (CATATG) vector backbone (not included): pFLAG-1, ompA signal peptide removed nt 4-1854: coding sequence GD5 fusion protein nt 7-30 FLAG peptide nt 37-54: 6 x His tag nt 55-492: GAL4 residues 2 to 147 (DNA binding domain) nt 505-1071 DT-B translocation domain nt 1093-1815: scFv(FRP5) (anti-ErbB2)

Fig.1 GD5

Click the linking at the following for detailed information https://2014.igem.org/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify lick the linking at the following for summarized information https://2014.igem.org/Team:SUSTC-Shenzhen/Project/A-B_toxin#Construction


pWF47-TEG (partial) Fominaya et al. 1998 (corrected 2014-07-31) Brief introduction: pWF47-TEG encodes the chimeric fusion protein TEG working as non-viral vector that can transfer specific target gene into specific target cells.

partial sequence (coding sequence) beginning with second half of NdeI site (catATG) vector backbone (not included): pFLAG-1 nt 1-1089: coding sequence TEG fusion protein nt 1-63: ompA signal peptide (pFLAG-1) nt 64-87: FLAG peptide (pFLAG-1) nt 100-249: hTGFalpha residues 40 to 89 (mature form) nt 259-276: 6 x His tag nt 280-615: ETA domain II nt 628-1065: GAL4 residues 2 to 147 (DNA binding domain) nt 1078-1089: KDEL ER retention sequence

Fig.2 TEG

Click the linking at the following for detailed information https://2014.igem.org/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify lick the linking at the following for summarized information https://2014.igem.org/Team:SUSTC-Shenzhen/Project/A-B_toxin#Construction

Target-sequence

Target-sequence_for_HIV Target-sequence for HIV Map.png

Target-sequence_for_HBV2 Target-sequence for HBV2 Map.png

Target-sequence_for_HBV1-2 Target-sequence for HBV1-2 Map.png

Maintained by the iGEM team SUSTC-Shenzhen.

Licensed under CC BY 4.0.

  • ©2014 SUSTC-Shenzhen

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