Team:PoznanBioInf

From 2014.igem.org

(Difference between revisions)
 
(71 intermediate revisions not shown)
Line 1: Line 1:
-
__TOC__
+
{{Template2}}
<html>
<html>
-
  <head>
+
<style>
-
    <meta charset="utf-8">
+
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
-
    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+
@font-face {
-
    <meta name="viewport" content="width=device-width, initial-scale=1">
+
font-family: PTSans;
-
    <title>Bootstrap 101 Template</title>
+
src: url(https://static.igem.org/mediawiki/2014/0/02/PT_Sans-Narrow-Web-Regular.ttf);
-
+
}
-
    <style type="text/css">
+
-
     #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .visualClear {display: none;} /*-- hides        default wiki settings --*/
+
body {
-
     h1 {display: none;}
+
     background: #000000;
-
    </style>
+
     font-family: PTSans;
 +
}
 +
</style>
 +
<!--  here ends the section that changes the default wiki template to a white full width background -->
-
    <!-- Bootstrap -->
+
<!-- beginning of your page -->
-
    <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/css/bootstrap.min.css">
+
-
+
-
    <!-- HTML5 Shim and Respond.js IE8 support of HTML5 elements and media queries -->
+
-
    <!-- WARNING: Respond.js doesn't work if you view the page via file:// -->
+
-
    <!--[if lt IE 9]>
+
-
      <script src="https://oss.maxcdn.com/html5shiv/3.7.2/html5shiv.min.js"></script>
+
-
      <script src="https://oss.maxcdn.com/respond/1.4.2/respond.min.js"></script>
+
-
    <![endif]-->
+
-
  </head>
+
-
  <body>
+
-
 
+
-
    <div class="container">
+
-
            <div class="navbar navbar-default">
+
-
<div class="container">
+
-
                  <div class="navbar-header">
+
-
    <a class="navbar-brand" href="/Team:Poznan-BioInf">Home</a>
+
-
                    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-responsive-collapse">
+
-
                      <span class="icon-bar"></span>
+
-
                      <span class="icon-bar"></span>
+
-
                      <span class="icon-bar"></span>
+
-
                    </button>
+
-
                  </div>
+
-
                  <div class="navbar-collapse collapse navbar-responsive-collapse">
+
-
                    <ul class="nav navbar-nav">
+
-
                      <li><a href="/Team:Poznan-BioInf/Team">Team</a></li>
+
-
  <li class="dropdown">
+
-
                        <a href="#" class="dropdown-toggle" data-toggle="dropdown">Project<b class="caret"></b></a>
+
-
                        <ul class="dropdown-menu">
+
-
                         
+
-
                          <li><a href="/Team:Poznan-BioInf/Introduction"">Introduction</a></li>
+
-
                          <li><a href="/Team:Poznan-BioInf/Book"">Lab book</a></li>
+
-
                          <li><a href="/Team:Poznan-BioInf/Funding">Funding</a></li>
+
-
                        </ul>
+
-
                      </li>
+
-
  <li><a href="/Team:Poznan-BioInf/Parts">Parts submitted</a></li>
+
-
  <li><a href="/Team:Poznan-BioInf/Safety">Safety</a></li>
+
-
  <li><a href="/Team:Poznan-BioInf/Human">Human practice</a></li>
+
-
                    </ul>
+
<!-- menu -->
-
                  </div><!-- /.nav-collapse -->
+
<div id="menu" style="margin-top:30px">
-
                </div><!-- /.container -->
+
  <ol >
-
              </div><!-- /.navbar -->
+
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Project">PROJECT</a></li>
-
                </div><!-- /.container -->
+
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Notebook">NOTEBOOK</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Parts">PARTS</a></li>
 +
    <li style="padding-top: none; width: 140px; padding: 10px 5px; margin-top: 50px; border-top:none;"><a href="https://2014.igem.org/Team:PoznanBioInf" style = "padding: 0 0;"><img src="https://static.igem.org/mediawiki/2014/9/96/PoznanBioInf_rec.png" style = "width: 130px;" /></a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Team">TEAM</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Attributions"">ATTRIB'S</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Attributions">CONTACT</a></li>
 +
  </ol>
 +
</div>
-
</div><!--bg-->
+
<!-- /menu -->
-
<div id="pages">
+
 
-
<div class="container">
+
 
-
<div class="well">
+
<!-- Page content begins -->
 +
 
 +
 
 +
<!-- navigation for project page-->
 +
<table style="width:100%; margin-top: 200px; margin-left:auto; margin-right:auto; text-align: center; background: none !important; cellspacing: none; position: absolute" cellpadding="20px" font-family="PTSans" >
 +
<style bgColor= "#000000"></style>
 +
<tr>
 +
<td  align = "center" width="500px"  height="400px" bgColor="#000000" style = "font-family: PTSans;"><img align = middle src="https://static.igem.org/mediawiki/2014/5/55/PoznanBioInf_LOGO.png" width="400px"></td>
 +
 
 +
<td width="600px"  height="300px"  bgColor="#000000" style="border-color: black !important; cellspacing: none;" >
 +
<h3><font color="white" style="font-family: PTSans; font-size: 20px;">REColi: Signal amplification and formattable bacterial memory by DNA edition.</font>
 +
</h3>
 +
 
 +
<font color="white" style="font-size:15px;">
 +
We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.
 +
</font></td>
 +
<td style="font-size: 20px; height: 40px; width: 100px;">    </td>
 +
</tr></table>
-
 
-
    <!-- jQuery (necessary for Bootstrap's JavaScript plugins) -->
 
-
    <script src="https://ajax.googleapis.com/ajax/libs/jquery/1.11.1/jquery.min.js"></script>
 
-
    <!-- Include all compiled plugins (below), or include individual files as needed -->
 
-
    <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/js/bootstrap.min.js"></script>
 
-
  </body>
 
</html>
</html>

Latest revision as of 03:55, 18 October 2014

REColi: Signal amplification and formattable bacterial memory by DNA edition.

We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.

Retrieved from "http://2014.igem.org/Team:PoznanBioInf"