Team:PoznanBioInf

From 2014.igem.org

(Difference between revisions)
 
(102 intermediate revisions not shown)
Line 1: Line 1:
-
{{CSS/Main}}
+
{{Template2}}
-
 
+
-
 
+
<html>
<html>
 +
<style>
 +
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
 +
@font-face {
 +
font-family: PTSans;
 +
src: url(https://static.igem.org/mediawiki/2014/0/02/PT_Sans-Narrow-Web-Regular.ttf);
 +
}
-
<!--main content -->
+
body {
-
<table width="70%" align="center">
+
    background: #000000;
 +
    font-family: PTSans;
 +
}
 +
</style>
 +
<!--  here ends the section that changes the default wiki template to a white full width background -->
-
<!--welcome box -->
+
<!-- beginning of your page -->
-
<tr>
+
-
<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
+
-
<h2>MUFASA: Multiple fragments assembler for scarless cloning of big genetic constructs.</h2>
+
-
<p>
+
-
Overlap-based cloning techniques like Gibson Assembly and Circular Polimerase Extension Cloning (CPEC) have been recently becoming more and more popular. While available software allows rapid overlap design for constructs of conventional size, details of its functioning are not always fully transparent. MUFASA aims not only at facilitating the scarless cloning from the oligonucleotide design to putting the tubes into the thermocycler, but also at designing the fragments themselves in parallel with the overlaps for synthesis of big constructs de novo. Use of multiple overlaps pose a risk of non-specific hybridization and overlap “shadowing”. Such “shadowy places” may be eliminated by thermodynamic sequence optimization of both CPEC primers and fragments of the desired construct.
+
-
</p>
+
-
<br>
+
-
<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:PoznanBioInf&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
+
-
</td>
+
-
</tr>
+
-
<tr> <td colspan="3" height="5px"> </td></tr>
+
<!-- menu -->
-
<!-- end welcome box -->
+
<div id="menu" style="margin-top:30px">
-
<tr>  
+
  <ol >
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Project">PROJECT</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Notebook">NOTEBOOK</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Parts">PARTS</a></li>
 +
    <li style="padding-top: none; width: 140px; padding: 10px 5px; margin-top: 50px; border-top:none;"><a href="https://2014.igem.org/Team:PoznanBioInf" style = "padding: 0 0;"><img src="https://static.igem.org/mediawiki/2014/9/96/PoznanBioInf_rec.png" style = "width: 130px;" /></a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Team">TEAM</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Attributions"">ATTRIB'S</a></li>
 +
    <li><a href="https://2014.igem.org/Team:PoznanBioInf/Attributions">CONTACT</a></li>
 +
  </ol>
 +
</div>
-
<!--navigation menu -->
+
<!-- /menu -->
-
<td align="center" colspan="3">
+
-
<table  width="100%">
 
-
<tr heigth="15px"></tr>
 
-
<tr heigth="75px">
 
 +
<!-- Page content begins -->
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
 
-
<a href="https://2014.igem.org/Team:PoznanBioInf"style="color:#000000">Home </a> </td>
 
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>  
+
<!-- navigation for project page-->
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Team"style="color:#000000"> Team </a> </td>
+
<table style="width:100%; margin-top: 200px; margin-left:auto; margin-right:auto; text-align: center; background: none !important; cellspacing: none; position: absolute" cellpadding="20px" font-family="PTSans" >
-
 
+
<style bgColor= "#000000"></style>
-
<td style="border:1px solid black;" align="center"  height ="45px"  onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://igem.org/Team.cgi?year=2014&team_name=PoznanBioInf"style="color:#000000"> Official Team Profile </a></td>
+
-
 
+
-
<td style="border:1px solid black" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Project"style="color:#000000"> Project</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Parts"style="color:#000000"> Parts</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Modeling"style="color:#000000"> Modeling</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center" height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7> 
+
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Notebook"style="color:#000000"> Notebook</a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Safety"style=" color:#000000"> Safety </a></td>
+
-
 
+
-
<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
+
-
<a href="https://2014.igem.org/Team:PoznanBioInf/Attributions"style="color:#000000"> Attributions </a></td>
+
-
 
+
-
 
+
-
<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
<!--end navigation menu -->
+
-
</tr>
+
-
 
+
-
 
+
-
</tr>
+
-
+
-
 
+
-
 
+
-
 
+
-
 
+
-
</td>
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1px"> </tr>
+
-
<tr> <td colspan="3"  height="5px"> </td></tr>
+
-
 
+
-
 
+
-
<!--requirements section -->
+
-
<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
+
<tr>
<tr>
-
<td width="45%" valign="top">
+
<td  align = "center" width="500px"  height="400px" bgColor="#000000" style = "font-family: PTSans;"><img align = middle src="https://static.igem.org/mediawiki/2014/5/55/PoznanBioInf_LOGO.png" width="400px"></td>
-
 
+
-
<p> Please be sure to keep these links, your audience will want to find your: </p>
+
-
 
+
-
<!-- Links to other team pages -->
+
-
<ul>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf">Home</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Team">Team</a> </li>
+
-
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=PoznanBioInf">Official Team Profile</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Project">Project</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Parts">Parts</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Modeling">Modeling</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Notebook">Notebook</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Safety">Safety</a> </li>
+
-
<li><a href="https://2014.igem.org/Team:PoznanBioInf/Attributions">Attributions</a> </li>
+
-
 
+
-
</ul>
+
-
 
+
-
</td>
+
-
 
+
-
<td > </td>
+
-
<td width="45%">
+
-
 
+
-
<p>There are a few wiki requirements teams must follow:</p>
+
-
<ul>
+
-
<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
+
-
<li>All pages must be created under the team’s name space.</li>
+
-
<li>As part of your documentation, keep the links from the menu to the left. </li>
+
-
<li>Do not use flash in wiki code. </li>
+
-
<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
+
-
</ul>
+
-
<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
+
-
 
+
-
</td>
+
-
</tr>
+
-
 
+
-
 
+
-
<tr> <td colspan="3"  height="15px"> </td></tr>
+
-
<tr><td bgColor="#e7e7e7" colspan="3" height="1"> </tr>
+
-
 
+
-
 
+
-
<!--tips  -->
+
-
<tr><td colspan="3" > <h3> Tips  </h3></td></tr>
+
-
 
+
-
<tr>
+
-
<td width="45%" valign="top">
+
-
<p>We are currently working on providing teams with some easy to use design templates.
+
-
<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
+
-
 
+
-
<ul>
+
-
<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
+
-
<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
+
-
<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
+
-
</ul>
+
-
 
+
-
<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
+
-
</td>
+
-
<td> </td>
+
<td width="600px"  height="300px"  bgColor="#000000" style="border-color: black !important; cellspacing: none;" >
-
<td width="45%">  
+
<h3><font color="white" style="font-family: PTSans; font-size: 20px;">REColi: Signal amplification and formattable bacterial memory by DNA edition.</font>
 +
</h3>
-
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
<font color="white" style="font-size:15px;">
 +
We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.
 +
</font></td>
 +
<td style="font-size: 20px; height: 40px; width: 100px;">    </td>
 +
</tr></table>
-
<ul>
+
</html>
-
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
-
<li>Be clear about what you are doing and what you plan to do.</li>
+
-
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
-
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
-
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
-
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
+
-
<li>Have lots of fun! </li>
+
-
</ul>
+
-
</br>
+
-
</td>
+
-
</tr>
+
-
</table>
+

Latest revision as of 03:55, 18 October 2014

REColi: Signal amplification and formattable bacterial memory by DNA edition.

We have designed a synthetic digital device inspired by electronic circuits called multiplexers. Its 3-bit memory allows saving and processing binary inputs in E. coli. Induced expression of serine recombinases, capable of specific DNA editing, enables construction of biological analogues of transistors - transcriptors - and their use as elementary memory units called SR-latches. The fourth, strobe signal resets the system to its original state. We have shown that the system could efficiently store data about previous contact with inducing sugars across hundreds of generations. This memory unit offers high sensitivity in inducer detection and signal amplification, allowing cheap induction or further development and use as a trace contaminants sensor. Eight possible output combinations, reported as RGB fluorescent proteins, may also turn out to be useful for complex coexpression research.

Retrieved from "http://2014.igem.org/Team:PoznanBioInf"