Team:UANL Mty-Mexico/project/The Whole Scheme
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<br>The DNA suppression system we chose makes use of TALENs and ZFNs [2]. Both, TALENs and ZFNs, are engineered proteins able to recognize specific sequences and bind to them. A nuclease attached to the binding domain then is capable to cause a nick in the DNA. If coupled strategically, these proteins can be used to digest the DNA as restriction enzymes. | <br>The DNA suppression system we chose makes use of TALENs and ZFNs [2]. Both, TALENs and ZFNs, are engineered proteins able to recognize specific sequences and bind to them. A nuclease attached to the binding domain then is capable to cause a nick in the DNA. If coupled strategically, these proteins can be used to digest the DNA as restriction enzymes. | ||
- | After we joined the delivery and the suppression systems, the whole scheme ended as shown in Figure 1. Plasmids containing TALEN/ZFN sites must be used to program organisms intended to be released into the environment. If any organisms shall be hacked, the first step would be to generate a bacteriophage solution in the laboratory. This solution will contain the bacteriophages at the required concentration, and the bacteriophages themselves will contain a phagemid with the appropriate TALEN/ZFN gene and the new program, if any. | + | After we joined the delivery and the suppression systems, the whole scheme ended as shown in Figure 1. Plasmids containing TALEN/ZFN sites must be used to program organisms intended to be released into the environment. If any organisms shall be hacked, the first step would be to generate a bacteriophage solution in the laboratory. This solution will contain the bacteriophages at the required concentration, and the bacteriophages themselves will contain a phagemid with the appropriate TALEN/ZFN gene and the new program, if any.<br> |
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- | </ | + | In our case, fluorescent proteins were selected to represent the programs and monitor the hacking process (Figure 1B). Variable reporters can be used to monitor the hacking process in situ. |
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<br>[2] Strauβ A, Lahaye T. (2013) Zinc fingers, TAL effectors, or Cas9-based DNA binding proteins: what's best for targeting desired genome loci? Mol Plant. 6:1384-7. | <br>[2] Strauβ A, Lahaye T. (2013) Zinc fingers, TAL effectors, or Cas9-based DNA binding proteins: what's best for targeting desired genome loci? Mol Plant. 6:1384-7. | ||
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Latest revision as of 03:54, 18 October 2014
![](https://static.igem.org/mediawiki/2014/7/73/Bannernuevouanl2014.png)
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The Whole Scheme In order to be able to hack the genetic programming of a biological system, we need two main tools: i) a DNA delivery system and ii) a DNA suppression system. Both, the delivery and the supression systems should be very specific so that other organisms can not be damaged.
References
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