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| <h4><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#header">Protocols</a></h4> | | <h4><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#header">Protocols</a></h4> |
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- | <a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#self-made">Self-Made</a> | + | <a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols#standard">Standard</a> |
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- | <div class="ca-content">Overview</div>
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- | </a>
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- | <ul>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/background">Background</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/overview/solution">Our solution</a></li>
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- | <ul>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing">Fungal Sensing</a></li>
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- | <ul>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing">Wetlab</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/fungal_sensing">Greenhouse</a></li>
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- | </ul>
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- | </li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/kill-switch">Kill-Switch</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/gene_transfer">Gene Transfer</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">Model</a>
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- | <ul>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">Promoter Design</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">System Cost</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/model">System Stability</a></li>
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- | </ul>
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- | </li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/project/interlab">Interlab Study</a></li>
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- | </ul>
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- | <a href="https://2014.igem.org/Team:Wageningen_UR/outreach">
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- | <div class="ca-content">Outreach</div>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/policy">Policy</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/outreach/synenergene">Synenergene</a></li>
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- | <ul>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/members">Members</a>
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- | <ul>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/members#students">Students</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/members#">Supervisors</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/gallery">Gallery</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/attributions">Attributions</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/team/acknowledgements">Acknowledgements</a></li>
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- | </ul>
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- | <span id="ca-icon5"></span>
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- | <div class="ca-content">Safety</div>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/safety/environment">Environment</a></li>
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- | </ul>
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- | </li>
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- | <li>
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- | <a href="https://2014.igem.org/Team:Wageningen_UR/notebook">
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- | <div class="ca-content">Notebook</div>
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- | </a>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/journal">Journal</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/protocols">Protocols</a></li>
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- | <li><a href="https://2014.igem.org/Team:Wageningen_UR/notebook/links">Interesting Links</a></li>
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- | </ul>
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| <h1>Protocols</h1> | | <h1>Protocols</h1> |
| <br/> | | <br/> |
- | <p>During iGEM, we used several existing protocols. We also developed a number of new protocols. On this page, both can be found</p> | + | <p>All protocols used by the team are listed on this page and explained in PDF files. We have used standard iGEM protocols and protocols that we derived from literature or designed ourselves. </p> |
- | <br><br> | + | <br> |
- | <section id="original"> | + | <section id="standard"> |
- | <h2>Original Protocols</h2> | + | <h2>Custom Protocols</h2> |
| <br> | | <br> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/d/d0/Wageningen_UR_protocols_Mediabuffers.pdf">Media & Buffers</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/8/87/Wageningen_UR_protocols_ammoniumanalysis.pdf" target="_blank">Ammonium Analysis</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/8/8f/Wageningen_UR_protocols_Transformations.pdf">Transformations</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/0/07/Wageningen_UR_protocols_DAPGprotocols.pdf" target="_blank">DAPG Protocols</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/1/12/Wageningen_UR_protocols_Usingkits.pdf">Using Kits</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_FAgrowth.pdf" target="_blank">Fusarium Inhibition</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/e/eb/Wageningen_UR_protocols_Pcr.pdf">PCR</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/4/4b/Wageningen_UR_protocols_MgLgrowth.pdf" target="_blank">MgL Growth Protocol</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/1/15/Wageningen_UR_protocols_Restrictionligation.pdf">Restrictions & Ligations</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/7/7a/Wageningen_UR_protocols_Pyoverdinegrowth.pdf" target="_blank">Pyoverdine Measurement Protocol</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/b/b5/Wageningen_UR_protocols_Site_Directed_Mutagenesis.pdf">Mutagenesis</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/2/23/Wageningen_UR_protocols_Fusariuminhibition.pdf"target="_blank">Fusaric Acid inducible promoter growth experiment protocol</a></h3> |
| + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/7/7d/Wageningen_UR_protocols_Metabolicmodelingpathways.pdf" target="_blank">Metabolic Modeling Pathways</a></h3> |
| + | |
| + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/8/8e/Wageningen_UR_protocols_Promotercharacterisation.pdf" target="_blank">Promoter Characterisation Protocol</a></h3> |
| + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/3/32/Wageningen_UR_protocols_Promoterassembly.pdf" target="_blank">Promoter Assembly</a></h3> |
| + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_congoredassay.pdf" target="_blank">Congo Red Assay</a></h3><p style="padding-left:40px;">Collaboration protocol from iGEM Delft-Leiden 2014</p> |
| </section> | | </section> |
- | <br><br>
| |
- | </section id="self-made">
| |
- | <h2>Self-Made Protocols</h2>
| |
| <br> | | <br> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/8/87/Wageningen_UR_protocols_ammoniumanalysis.pdf">Ammonium Analysis</a></h3> | + | </section id="original"> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/0/07/Wageningen_UR_protocols_DAPGprotocols.pdf">DAPG Protocols</a></h3> | + | <h2>Standard Protocols</h2> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/1/14/Wageningen_UR_protocols_FAgrowth.pdf">Fusarium Inhibition</a></h3> | + | <br> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/4/4b/Wageningen_UR_protocols_MgLgrowth.pdf">MgL Growth Protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/d/d0/Wageningen_UR_protocols_Mediabuffers.pdf" target="_blank">Media & Buffers</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/7/7a/Wageningen_UR_protocols_Pyoverdinegrowth.pdf">Pyoverdine Growth Protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/8/8f/Wageningen_UR_protocols_Transformations.pdf" target="_blank">Transformations</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/2/23/Wageningen_UR_protocols_Fusariuminhibition.pdf">Fusaric Acid inducible promoter growth experiment protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/12/Wageningen_UR_protocols_Usingkits.pdf" target="_blank">Used Kits</a></h3> |
- | <h3><a href="https://static.igem.org/mediawiki/2014/8/8e/Wageningen_UR_protocols_Promotercharacterisation.pdf">Promoter Characterisation Protocol</a></h3> | + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/e/eb/Wageningen_UR_protocols_Pcr.pdf" target="_blank">PCR</a></h3> |
| + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/1/15/Wageningen_UR_protocols_Restrictionligation.pdf" target="_blank">Restrictions & Ligations</a></h3> |
| + | <h3><a class="soft_link" href="https://static.igem.org/mediawiki/2014/b/b5/Wageningen_UR_protocols_Site_Directed_Mutagenesis.pdf" target="_blank">Mutagenesis</a></h3> |
| </section> | | </section> |
- |
| + | <br> |
- | | + | <p style="float:right"><b>Continue to <a href="https://2014.igem.org/Team:Wageningen_UR/notebook/links" class="soft_link">Interesting links >></a> </b> |
- |
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- | <p>iGEM Wageningen UR 2014. Follow us on <a class="soft_link" href="http://twitter.com/igemwageningen">Twitter</a> and <a class="soft_link" href="http://www.facebook.com/iGEMWageningen">Facebook</a>!
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- | <br />
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- | For more information about iGEM, check out their <a class="soft_link" href="https://igem.org">website</a> and <a class="soft_link" href="http://twitter.com/igem">Twitter</a>!</p>
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| + | |
- | }
| + | |
- |
| + | |
- | if(windowPos + windowHeight == docHeight) {
| + | |
- | if (!$("sidenav li:last-child a").hasClass("sidenav-active")) {
| + | |
- | var sidenavActiveCurrent = $(".sidenav-active").attr("href");
| + | |
- | $("a[href='" + sidenavActiveCurrent + "']").removeClass("sidenav-active");
| + | |
- | $("sidenav li:last-child a").addClass("sidenav-active");
| + | |
- | }
| + | |
- | }
| + | |
- | });
| + | |
- | });
| + | |
- | </script>
| + | |
- |
| + | |
- | <script>
| + | |
- | $(document).ready(function() {
| + | |
- | $.timeliner({
| + | |
- | startOpen:['#02w1EX', '#02w2EX'] <!--Initial opened journal-->
| + | |
- | });
| + | |
- | $.timeliner({
| + | |
- | timelineContainer: '#timelineContainer_2'
| + | |
- | });
| + | |
- | // Colorbox Modal
| + | |
- | $(".CBmodal").colorbox({inline:true, initialWidth:100, maxWidth:682, initialHeight:100, transition:"elastic",speed:750});
| + | |
- | });
| + | |
- | </script>
| + | |
- |
| + | |
- | <script>
| + | |
- | $(document).ready(function() {
| + | |
- | $(".onoffswitch-checkbox").click(function() {
| + | |
- | $(".overview >div img.top").toggleClass("transparent");
| + | |
- | });
| + | |
- | });
| + | |
- | </script>
| + | |
- |
| + | |
- | </body>
| + | |
| </html> | | </html> |
| + | {{:Team:Wageningen_UR/templates/footer}} |