Team:Aachen/Safety

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{{Team:Aachen/Header}}
{{Team:Aachen/Header}}
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= Biosafety =
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[[File:Aachen_14-10-13_Pathogen_Cell_iNB.png|right|150px]]
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DRAFT: ''The Aachen team is committed to consider all aspects of the entire project, including biosafety.''
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= Safety =
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Our iGEM team is committed to consider all aspects of the entire project, especially biosafety. For this project, two biosafety aspects have to be covered. On the one hand, we are using ''E. coli'' as '''genetically modified organism''', and on the other hand, we are detecting ''Pseudomonas aeruginosa'', an '''opportunistic human pathogen'''. It infects people suffering from cystic fibrosis or immunodeficiency as well as severe burns or open wounds. When sampling ''P. aeruginosa'', we should prevent proliferation and spread of the bacterium. For ''E. coli'', we have to take care of biological containment of a genetically modified organism.
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For our project two biosafety aspects have to be consider. On the one hand we are using E.coli as genetically modified organism and on the other ahnd we are detecting Pseudomonas aeruginosa a human pathogen. While using Pseudomonas we have the problem of non-proliferation. For E.coli we have to look after the non-release of genetically modified organisms. In general we developed and designed the measurenment device as closed system for a better safety handling. In  this way neither the sampled pathogens nor the genetically modified sensor cells can escape out of our biosensor unit. Also the electronic components are inaccessible so that no one could get an electric shock. For the detection we are using one way sampling and sensor chips which can be disposed after autoclaving or irradate them with a strong UV light.
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In general, we developed and designed the measurement device as '''closed system''' for a better safety handling. This way, neither the sampled pathogens nor the genetically modified sensor cells can escape our biosensor unit. For the detection, we are using one-time usage sampling and sensor chips which can be disposed of after '''autoclaving or irradation''' with strong UV light. Moreover, the electronic components are in a separate compartment and inaccessible for the user, preventing electric shock or other injuries.
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Pseudomonas aeruginosa
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<center>
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* get the pure agar chip on a solid surface to improve contamination with Pseudomonas
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{{Team:Aachen/Figure|Aachen_Security_WatsOn.png|title=Biosafety level for ''WatsOn''|subtitle=Don't forget to use ''WatsOn'' only in laboratoris with the biosafty standard 1|width=500px}}
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* put the sampling chip into the measurement device for detection
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</center>
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* don't get this sampling chip out of the device until you finished detection
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To detect ''P. aeruginosa'', a sampling agar chip is slightly pressed against the solid surface to be tested. This sampling chip is put on the sensor chip and both are placed in a petri dish which is closed thoroughly. Thereby, the cells have no contact with the device during or after the measurement. However, the sensor chips must be handled in '''S1 environments only''' since they contain genetically modified ''E. coli''. Afterwards, both chips can be autoclaved and disposed. The whole lining of the measurement device is built from plastic so that it can be disinfected easily.
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<!-- Afterwards, the sampling chip is immediately introduced into our measurement device and will not be removed until the detection is finished and the chips have been disinfected. The sensor chips must be handled in '''S1 environments only''' since they contain genetically modified ''E. coli''. However, once introduced into the measurement device, the sensor chips, too, will not be removed before disinfection. The living cells inside the measurement device are effectively killed after a measurement by '''using desinfectants''' such as Bacillol. For this procedure, the drawer of the measurement device is opened and Bacillol is poured over the sampling and sensor chips. -->
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To '''simulate the worst case scenario''', we did replica plating of some exemplary sensor chips. In three experiments, we got an arithmetic mean of five colonies which were picked up. From that we concluded that the '''risk of infection is really low''' even if the measurement device and chips are not handled properly.
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For further analysis of our project from a safety perspective, please view our [https://igem.org/Safety/Safety_Form?team_id=1319 safety form].
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E.coli
 
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* build a sensor chip including E.coli cells which have to be handled in S1 laboratories
 
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* that is the reason why it's only allowed to use the measurement device in S1 labs
 
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* put the sensor chip into the device like the sampling chip before
 
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* don't get the sensor chip out of the device until you finished detection
 
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The living cells inside the measurement device are ensured to be killed after detection at first by using desinfectant like bacillol. For this procedure open teh drawer of the measurement device and pour bacillol on the sampling and also on the sensor chip. After this measures both chips can be autoclaved and later disposed.
 
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For the worst case scenario we made a stamp pront of the used sensor chip. In three experiments we got an arithmetic mean of five colonies which could get out of these chip. To conclude the infection risk is really low if you are not using the measurement device as descripted.
 
{{Team:Aachen/Footer}}
{{Team:Aachen/Footer}}

Latest revision as of 03:51, 18 October 2014

Aachen 14-10-13 Pathogen Cell iNB.png

Safety

Our iGEM team is committed to consider all aspects of the entire project, especially biosafety. For this project, two biosafety aspects have to be covered. On the one hand, we are using E. coli as genetically modified organism, and on the other hand, we are detecting Pseudomonas aeruginosa, an opportunistic human pathogen. It infects people suffering from cystic fibrosis or immunodeficiency as well as severe burns or open wounds. When sampling P. aeruginosa, we should prevent proliferation and spread of the bacterium. For E. coli, we have to take care of biological containment of a genetically modified organism.

In general, we developed and designed the measurement device as closed system for a better safety handling. This way, neither the sampled pathogens nor the genetically modified sensor cells can escape our biosensor unit. For the detection, we are using one-time usage sampling and sensor chips which can be disposed of after autoclaving or irradation with strong UV light. Moreover, the electronic components are in a separate compartment and inaccessible for the user, preventing electric shock or other injuries.

Aachen Security WatsOn.png
Biosafety level for WatsOn
Don't forget to use WatsOn only in laboratoris with the biosafty standard 1

To detect P. aeruginosa, a sampling agar chip is slightly pressed against the solid surface to be tested. This sampling chip is put on the sensor chip and both are placed in a petri dish which is closed thoroughly. Thereby, the cells have no contact with the device during or after the measurement. However, the sensor chips must be handled in S1 environments only since they contain genetically modified E. coli. Afterwards, both chips can be autoclaved and disposed. The whole lining of the measurement device is built from plastic so that it can be disinfected easily.

To simulate the worst case scenario, we did replica plating of some exemplary sensor chips. In three experiments, we got an arithmetic mean of five colonies which were picked up. From that we concluded that the risk of infection is really low even if the measurement device and chips are not handled properly.

For further analysis of our project from a safety perspective, please view our safety form.


Contact Disclaimer

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