Team:SUSTC-Shenzhen/Notebook/timeline.json

From 2014.igem.org

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{
{
-
    "timeline":
+
  "timeline": {
-
     {
+
     "headline": "Lab Notes",
-
         "headline":"The Main Timeline Headline Goes here",
+
    "type": "default",
-
         "type":"default",
+
    "text": "<p>The steps to success.</p>",
-
         "text":"<p>Intro body text goes here, some HTML is ok</p>",
+
    "asset": {
 +
      "media": "https://static.igem.org/mediawiki/2014/2/22/Logo2014v2.png",
 +
      "credit": "iGEM SUSTC-Shenzhen",
 +
      "caption": "Lab Notes"
 +
    },
 +
    "date": [
 +
      {
 +
         "startDate": "2014,7,27",
 +
         "endDate": "2014,7,28",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Fundamental/TransEfficiency.htm'>Effiency Test</a>",
 +
         "text": "<p>DH5α&BL21</p>",
 +
        "tag": "Fundamental",
 +
        "classname": "",
         "asset": {
         "asset": {
-
            "media":"http://yourdomain_or_socialmedialink_goes_here.jpg",
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          "media": "https://static.igem.org/mediawiki/2014/6/6c/SUSTC-Shenzhen-20140728_21-30_DH5a.jpg",
-
            "credit":"Credit Name Goes Here",
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          "thumbnail": "",
-
            "caption":"Caption text goes here"
+
          "credit": "ProfFan",
-
         },
+
          "caption": "Figure 1"
-
         "date": [
+
        }
-
            {
+
      },
-
                "startDate":"2011,12,10",
+
      {
-
                "endDate":"2011,12,11",
+
        "startDate": "2014,8,17",
-
                "headline":"Headline Goes Here",
+
        "endDate": "2014,8,22",
-
                "text":"<p>Body text goes here, some HTML is OK</p>",
+
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-puromycin-for-cell-selection'>Determining the appropriate concentration of puromycin for cell selection</a>",
-
                "tag":"This is Optional",
+
        "text": "<p>Prepare for selection</p>",
-
                "classname":"optionaluniqueclassnamecanbeaddedhere",
+
        "tag": "HeLa Cell",
-
                "asset": {
+
        "classname": "",
-
                    "media":"http://twitter.com/ArjunaSoriano/status/164181156147900416",
+
        "asset": {
-
                    "thumbnail":"optional-32x32px.jpg",
+
          "media": "https://static.igem.org/mediawiki/2014/3/3e/SUSTC-Shenzhen-Puromycin-4.PNG",
-
                    "credit":"Credit Name Goes Here",
+
          "thumbnail": "",
-
                    "caption":"Caption text goes here"
+
          "credit": "Lidan Zeng",
-
                }
+
          "caption": "Our cells!"
-
            }
+
         }
-
         ],
+
      },
-
         "era": [
+
      {
-
            {
+
         "startDate": "2014,7,18",
-
                "startDate":"2011,12,10",
+
        "endDate": "2014,7,18",
-
                "endDate":"2011,12,11",
+
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-II-for-plasmid-pX330-EGFP'>Plasmid amplification II for pX330 & EGFP & verifications by electrophoresis</a>",
-
                "headline":"Headline Goes Here",
+
        "text": "<p>Prepare for selection</p>",
-
                "text":"<p>Body text goes here, some HTML is OK</p>",
+
        "tag": "CRISPR",
-
                "tag":"This is Optional"
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        "classname": "",
-
            }
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        "asset": {
-
 
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          "media": "https://static.igem.org/mediawiki/2014/f/f2/SUSTC-Image-Plasmid-AmpII-1.png",
-
         ]
+
          "thumbnail": "",
-
     }
+
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "Electrophoresis Snapshot"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,9",
 +
        "endDate": "2014,8,10",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Enzyme-digestion-agrose-gel-DNA-Extraction-for-PX330-vector'>Enzyme digestion & agrose gel DNA Extraction for PX330 vector</a>",
 +
        "text": "<p>To prepare PX330 vector for the following ligation experiments</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,6",
 +
        "endDate": "2014,8,6",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/5*UAS-plasmids-Fill-in'>5*UAS plasmids Fill-in</a>",
 +
        "text": "<p>To eliminate the EcoRI site on the 5*UAS plasmid</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/1/10/SUSTC-Image-5UAS-Fillin-1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "Eluted DNA"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,10",
 +
        "endDate": "2014,8,10",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>",
 +
        "text": "<p>To get our target fragment of mCherry by PCR</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/thumb/0/00/SUSTC-Image-PCR-mCherry-1.jpg/800px-SUSTC-Image-PCR-mCherry-1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "Eluted DNA"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,9",
 +
        "endDate": "2014,8,10",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-second-time'>UAS Fill-in (The second\ntime)</a>",
 +
        "text": "<p>The same as the first Fill-in experiment</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/6/63/SUSTC-Shenzhen-UAS-2nd-1.PNG",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "Eluted DNA"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,11",
 +
        "endDate": "2014,8,11",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Struggles-for-our-second-time-Fill-in'>Struggles for our second-time Fill-in</a>\n",
 +
        "text": "<p>A real challenge, indeed.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,11",
 +
        "endDate": "2014,8,11",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-plasmid-PX330-MCherry-GAL4-PX330-MCherry-amplified-2014-8-9-by-restriction-digestion'>Verification for plasmid PX330-MCherry-GAL4,PX330-MCherry (amplified on 2014.8.9) by restriction digestion</a>",
 +
        "text": "<p>Failure, as it is.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/thumb/0/0c/SUSTC-Shenzhen-Verification-pX330-140809-1.PNG/800px-SUSTC-Shenzhen-Verification-pX330-140809-1.PNG",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,12",
 +
        "endDate": "2014,8,12",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-verification-of-plasmid-PX330-MCherry-GAL4'>Follow-up for verification of plasmid PX330-MCherry-GAL4 and The results of 'Struggles for fill-in(the second time)'</a>",
 +
        "text": "<p>Life full of cycles, but each iteration with happiness.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014//f/fd/SUSTC-Shenzhen-verification-for-pX330-mCherry-and-pX330-mCherry-GAL4.PNG",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "Eluted DNA"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,12",
 +
        "endDate": "2014,8,12",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-Struggles-for-fill-in-the-second-time'>Follow-up for 'struggles for our second-time Fill-in'</a>",
 +
        "text": "<p>Failure, again.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,13",
 +
        "endDate": "2014,8,16",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-The-third-time'>UAS Fill-in (The-third-time)</a>",
 +
        "text": "<p>Then try for another time.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/f/f9/SUSTC-Shenzhen-varification-for-digested-plasmid.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "Plates without Colonies"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,14",
 +
        "endDate": "2014,8,14",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verifications-for-struggles-for-our-second-time-Fill-in'>Verifications for 'struggles for our second-time Fill-in'</a>",
 +
        "text": "<p>Results verified.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/7/7c/SUSTC-Shenzhen-Verifications-for-succeed-Fill-in-plasmid.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
         "startDate": "2014,8,18",
 +
         "endDate": "2014,8,20",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Successful-fill-in-plasmid-(G56-1)-amplification'>Successful fill-in plasmid (G56-1) amplification</a>",
 +
        "text": "<p>Days of success.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,20",
 +
        "endDate": "2014,8,20",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-UAS'>Point-mutation PCR for UAS</a>",
 +
        "text": "<p>One approach.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/3/35/SUSTC-Shenzhen-Vatification-of-PCR-pointmutation-for-UAS.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,21",
 +
        "endDate": "2014,8,21",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-mCherry-with-BbsI-site-mutation'>Point-mutation PCR for mCherry (with BbsI site mutation)</a>",
 +
        "text": "<p>To eliminate the BbsI site on mCherry fragment.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/d/de/SUSTC-Shenzhen-Point-mutation-PCR-for-mCherry1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel under UV Light"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,21",
 +
        "endDate": "2014,8,22",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Endotoxin-Free-plasmid-purification-for-pX330-mCherry-pX330-mCherry-GAL4'>Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4</a>",
 +
        "text": "<p>To prepare for further cell culturing.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,22",
 +
        "endDate": "2014,8,22",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-pX330-mCherry-pX330-mCherry-GAL4-enzyme-digestion'>Verification for pX330+mCherry, pX330+mCherry+GAL4 by enzyme digestion</a>",
 +
        "text": "<p>To ensure that we have not made errors.</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/8/85/SUSTC-Shenzhen-Verification-for-pX330-mCherry-pX330-mCherry-GAL4-by-enzyme-digestion.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,25",
 +
        "endDate": "2014,8,26",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Construction-5X-UAS-mCherry-pX330-7X-UAS-mCherry-pX330-I'>Construction of 5X-UAS-mCherry-pX330 & 7X-UAS-mCherry-pX330 I</a>",
 +
        "text": "<p></p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,7,17",
 +
        "endDate": "2014,7,19",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-I-for-plasmid-pX330-EGFP'>Plasmid amplification I for plasmid pX330 & EGFP</a>",
 +
        "text": "<p>Prepare the necessary materials</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,04",
 +
        "endDate": "2014,8,05",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Thawing-HeLa-Cell'>Thawing HeLa Cell</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,14",
 +
        "endDate": "2014,9,20",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Construct-Cellline'>Constructing Cell Line</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/2/2d/SUSTC-Shenzhen-5DayAfterTransefation-8.20G1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "G1 pool cells on petri dish"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,7,27",
 +
        "endDate": "2014,8,10",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/PB5-TetOn-Cas9-PB3-plasmid-construction'>PB5-TetOn-Cas9-PB3 Plasmid Construction</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-PB5-Puro-TetOn-Cas9-PB3.png",
 +
          "thumbnail": "",
 +
          "credit": "ProfFan",
 +
          "caption": "Figure 1"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,27",
 +
        "endDate": "2014,10,06",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-higher-concentration-of-balsticidin-for-cell-selection'>Determining the Increased Concentration of Balsticidin for Cell Selection</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/a/ac/SUSTC-Shenzhen-Blasticidin_testing_III3.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "Strains of HeLa Cells"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,26",
 +
        "endDate": "2014,8,26",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Struggles I for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/2/2a/SUSTC-Shenzhen-Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-%26-7X-UAS-mCherry-pX330-1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,27",
 +
        "endDate": "2014,8,27",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-MU2-and-Struggles-II-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Plasmid purification for MU2 and Struggles II for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/a/ae/SUSTC-Shenzhen-Purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL-1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,28",
 +
        "endDate": "2014,8,28",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL'>Plasmid purification for Struggle I and Struggle II and Verification with AgeI EcoRI and BbsI for MU2 BIG and SMALL</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/a/ae/SUSTC-Shenzhen-Purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL-1.jpg",
 +
          "thumbnail": "",
 +
          "credit": "ProfFan",
 +
          "caption": "Figure 1"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,29",
 +
        "endDate": "2014,8,29",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-no-endotoxin-for-BIG-and-SMALL'>Plasmid purification no endotoxin for BIG and SMALL</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {}
 +
      },
 +
      {
 +
        "startDate": "2014,8,30",
 +
        "endDate": "2014,8,30",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_replaceable_0X_2X_5X_7X_UAS-mCherry-pX330_plasmids'>Construction of gRNA replaceable 0X/2X/5X/7X-UAS-mCherry-pX330 plasmids</a>",
 +
        "text": "<p></p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/f/f6/SUSTC-Shenzhen-Construction-of-gRNA-replaceable-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,31",
 +
        "endDate": "2014,8,31",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI'>Plasmid purification for 0X,2X,5X,7X-UAS-mCherry-pX330 plasmids and Verification with AgeI and BbsI</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/f/f6/SUSTC-Shenzhen-Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,10,6",
 +
        "endDate": "2014,10,10",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_for_HIV_or_HBV_carried_plasmids'>Construction of plasmids carrying the gRNA for HIV/HBV</a>",
 +
        "text": "<p>DH5α&BL21</p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/9/9b/SUSTC-Shenzhen-Construction-of-gRNA-for-HIV-or-HBV-carried-plasmids.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Mengqi Xu, Sijia Liu",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,22",
 +
        "endDate": "2014,10,12",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Parts_building'>Building Necessary Parts</a>",
 +
        "text": "<p>We build everything.</p>",
 +
        "tag": "Fundamental",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/e/e2/2014_SUSTC-Shenzhen_gel_result_0930.2.png",
 +
          "thumbnail": "",
 +
          "credit": "Rifei Chen",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,8",
 +
        "endDate": "2014,10,16",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Biobricks_Characterization'>Characterization of Biobricks</a>",
 +
        "text": "<p>Digging deeper into the nature of biology.</p>",
 +
        "tag": "Fundamental",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/6/6c/SUSTC-Shenzhen_Characterization_9.28_Gel.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Peng Peng",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,13",
 +
        "endDate": "2014,8,19",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/mCherry-BbsI-pointmutation'>mCherry BbsI point mutation</a>",
 +
        "text": "<p>one step for constructing a universal usable gRNA-insertion plasmid.</p>",
 +
        "tag": "Fundamental",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/d/de/SUSTC-Shenzhen-mCherry-BbsI-pointmutation-first-PCR-result.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Yicong Tao",
 +
          "caption": "DNA Gel"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,29",
 +
        "endDate": "2014,10,7",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Investigation-Cas9-protein-affect-cell-growth'>Investigation Cas9 protein affect cell growth</a>",
 +
        "text": "<p>Investigation of the effect of Cas9 protein on the cell growth(The safety of Cas9).</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/a/a9/Figure_1._The_cell_grow_curve.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "The Cell Growth Curve"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,13",
 +
        "endDate": "2014,9,21",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/G1_cells_transfected_with_px330'>Test of the function of Cas9</a>",
 +
        "text": "<p>Ensure the function of modified px330 targeting EGFP gene.</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/4/4e/SUSTC-Shenzhen-G1_cells_without_pX330_transfection.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "G1 Cells without pX330"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,13",
 +
        "endDate": "2014,9,24",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Test-of-the-clone-G3_6'>Test of the first clone of G3 (G3-6)</a>",
 +
        "text": "<p>Prepare for transfection with A-B toxin.</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/4/4e/SUSTCSUSTC-Shenzhen-EGFP-mCherry-fluorescence-G3-6-microscope.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "G3 under Fluorescence Microscope"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,20",
 +
        "endDate": "2014,10,7",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/The_quality_test_of_the_clones_of_G3%26G4'>The quality test of the clones of G3&G4</a>",
 +
        "text": "<p>Test the quality of clones we pick from G3 and G4.</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/d/de/SUSTC-Shenzhen-monoclone-quality-G3-G4-negative-ctrl.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "Normal HeLa Cells"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,14",
 +
        "endDate": "2014,9,25",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Examination-of-the-mCherry-expression-of-UAS_mCherry_gRNA'>Examination of the mCherry expression of the plasmids UAS-mCherry-gRNA</a>",
 +
        "text": "<p>Test the quality of UAS-mCherry-gRNA.</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-UAS_test_47hours_transfect_cells.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "Different Periods of G3 under FM"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,10,3",
 +
        "endDate": "2014,10,18",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/A-B-Toxin-transfect'>Transfection of cells with A-B toxin</a>",
 +
        "text": "<p>Introduce gRNA with TEG/GD5 protein.</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/c/cb/SUSTC-Shenzhen-UAS_test_47hours_transfect_cells.png",
 +
          "thumbnail": "",
 +
          "credit": "Lin Le",
 +
          "caption": "3 Days after Transfection"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,16",
 +
        "endDate": "2014,10,16",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify'>Mainstream A-B Toxin Preparation</a>",
 +
        "text": "<p>Purify the Protein and Meanwhile Improve the Protocol.</p>",
 +
        "tag": "A-B Toxin",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/4/46/SUSTC-Shenzhen-PWF47-TEG%28coding_sequence%29.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Longze Su",
 +
          "caption": "Coding Sequence"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,8,8",
 +
        "endDate": "2014,8,11",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLa_Cell/blasticidin_screening'>Blasticidin Screening</a>",
 +
        "text": "<p>To test the resistance of HeLa cell to blasticidin.</p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
          "media": "https://static.igem.org/mediawiki/2014/2/26/Spread.jpg",
 +
          "thumbnail": "",
 +
          "credit": "Longze Su",
 +
          "caption": "3 Days after Transfection"
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,6,12",
 +
        "endDate": "2014,7,27",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Preparation_work'>Experiment Training &amp; Plasmid Building</a>",
 +
        "text": "<p>Introduce gRNA with TEG/GD5 protein.</p>",
 +
        "tag": "Fundamental",
 +
        "classname": "",
 +
        "asset": {
 +
        }
 +
      },
 +
      {
 +
        "startDate": "2014,9,22",
 +
        "endDate": "2014,10,4",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/insert-target-to-pBX-083'>PCR &amp; Insert target-sequence to pBX-083</a>",
 +
        "text": "<p></p>",
 +
        "tag": "CRISPR",
 +
        "classname": "",
 +
        "asset": {
 +
        }
 +
      },
 +
      {
 +
         "startDate": "2014,8,27",
 +
        "endDate": "2014,8,27",
 +
        "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-blasticidin-for-cell-selection'>Determining the appropriate concentration of blasticidin for cell selection</a>",
 +
        "text": "<p></p>",
 +
        "tag": "HeLa Cell",
 +
        "classname": "",
 +
        "asset": {
 +
        }
 +
      }
 +
    ],
 +
     "era": [
 +
      {
 +
        "startDate": "2014,7,27",
 +
        "endDate": "2014,8.7",
 +
        "headline": "Basic Research",
 +
        "text": "<p>The Fundamentals</p>",
 +
        "tag": "Fundamental"
 +
      }
 +
    ]
 +
  }
}
}

Latest revision as of 03:48, 18 October 2014

{ 

 "timeline": {
   "headline": "Lab Notes",
   "type": "default",
"text": "

The steps to success.

", 
   "asset": {
     "media": "Logo2014v2.png",
     "credit": "iGEM SUSTC-Shenzhen",
     "caption": "Lab Notes"
   },
   "date": [
     {
       "startDate": "2014,7,27",
       "endDate": "2014,7,28",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Fundamental/TransEfficiency.htm'>Effiency Test</a>",
"text": "

DH5α&BL21

", 
       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-20140728_21-30_DH5a.jpg",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,8,17",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-puromycin-for-cell-selection'>Determining the appropriate concentration of puromycin for cell selection</a>",
"text": "

Prepare for selection

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Puromycin-4.PNG",
         "thumbnail": "",
         "credit": "Lidan Zeng",
         "caption": "Our cells!"
       }
     },
     {
       "startDate": "2014,7,18",
       "endDate": "2014,7,18",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-II-for-plasmid-pX330-EGFP'>Plasmid amplification II for pX330 & EGFP & verifications by electrophoresis</a>",
"text": "

Prepare for selection

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Image-Plasmid-AmpII-1.png",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Electrophoresis Snapshot"
       }
     },
     {
       "startDate": "2014,8,9",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Enzyme-digestion-agrose-gel-DNA-Extraction-for-PX330-vector'>Enzyme digestion & agrose gel DNA Extraction for PX330 vector</a>",
"text": "

To prepare PX330 vector for the following ligation experiments

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,6",
       "endDate": "2014,8,6",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/5*UAS-plasmids-Fill-in'>5*UAS plasmids Fill-in</a>",
"text": "

To eliminate the EcoRI site on the 5*UAS plasmid

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Image-5UAS-Fillin-1.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,10",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>",
"text": "

To get our target fragment of mCherry by PCR

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "800px-SUSTC-Image-PCR-mCherry-1.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,9",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-second-time'>UAS Fill-in (The second\ntime)</a>",
"text": "

The same as the first Fill-in experiment

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-UAS-2nd-1.PNG",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,11",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Struggles-for-our-second-time-Fill-in'>Struggles for our second-time Fill-in</a>\n",
"text": "

A real challenge, indeed.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,11",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-plasmid-PX330-MCherry-GAL4-PX330-MCherry-amplified-2014-8-9-by-restriction-digestion'>Verification for plasmid PX330-MCherry-GAL4,PX330-MCherry (amplified on 2014.8.9) by restriction digestion</a>",
"text": "

Failure, as it is.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "800px-SUSTC-Shenzhen-Verification-pX330-140809-1.PNG",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,12",
       "endDate": "2014,8,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-verification-of-plasmid-PX330-MCherry-GAL4'>Follow-up for verification of plasmid PX330-MCherry-GAL4 and The results of 'Struggles for fill-in(the second time)'</a>",
"text": "

Life full of cycles, but each iteration with happiness.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-verification-for-pX330-mCherry-and-pX330-mCherry-GAL4.PNG",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,12",
       "endDate": "2014,8,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-Struggles-for-fill-in-the-second-time'>Follow-up for 'struggles for our second-time Fill-in'</a>",
"text": "

Failure, again.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,13",
       "endDate": "2014,8,16",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-The-third-time'>UAS Fill-in (The-third-time)</a>",
"text": "

Then try for another time.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-varification-for-digested-plasmid.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Plates without Colonies"
       }
     },
     {
       "startDate": "2014,8,14",
       "endDate": "2014,8,14",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verifications-for-struggles-for-our-second-time-Fill-in'>Verifications for 'struggles for our second-time Fill-in'</a>",
"text": "

Results verified.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Verifications-for-succeed-Fill-in-plasmid.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,18",
       "endDate": "2014,8,20",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Successful-fill-in-plasmid-(G56-1)-amplification'>Successful fill-in plasmid (G56-1) amplification</a>",
"text": "

Days of success.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,20",
       "endDate": "2014,8,20",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-UAS'>Point-mutation PCR for UAS</a>",
"text": "

One approach.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Vatification-of-PCR-pointmutation-for-UAS.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,21",
       "endDate": "2014,8,21",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-mCherry-with-BbsI-site-mutation'>Point-mutation PCR for mCherry (with BbsI site mutation)</a>",
"text": "

To eliminate the BbsI site on mCherry fragment.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Point-mutation-PCR-for-mCherry1.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel under UV Light"
       }
     },
     {
       "startDate": "2014,8,21",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Endotoxin-Free-plasmid-purification-for-pX330-mCherry-pX330-mCherry-GAL4'>Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4</a>",
"text": "

To prepare for further cell culturing.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,22",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-pX330-mCherry-pX330-mCherry-GAL4-enzyme-digestion'>Verification for pX330+mCherry, pX330+mCherry+GAL4 by enzyme digestion</a>",
"text": "

To ensure that we have not made errors.

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Verification-for-pX330-mCherry-pX330-mCherry-GAL4-by-enzyme-digestion.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,25",
       "endDate": "2014,8,26",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Construction-5X-UAS-mCherry-pX330-7X-UAS-mCherry-pX330-I'>Construction of 5X-UAS-mCherry-pX330 & 7X-UAS-mCherry-pX330 I</a>",
"text": "

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,7,17",
       "endDate": "2014,7,19",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-I-for-plasmid-pX330-EGFP'>Plasmid amplification I for plasmid pX330 & EGFP</a>",
"text": "

Prepare the necessary materials

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,04",
       "endDate": "2014,8,05",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Thawing-HeLa-Cell'>Thawing HeLa Cell</a>",
"text": "

DH5α&BL21

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,14",
       "endDate": "2014,9,20",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Construct-Cellline'>Constructing Cell Line</a>",
"text": "

DH5α&BL21

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-5DayAfterTransefation-8.20G1.jpg",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "G1 pool cells on petri dish"
       }
     },
     {
       "startDate": "2014,7,27",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/PB5-TetOn-Cas9-PB3-plasmid-construction'>PB5-TetOn-Cas9-PB3 Plasmid Construction</a>",
"text": "

DH5α&BL21

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-PB5-Puro-TetOn-Cas9-PB3.png",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,9,27",
       "endDate": "2014,10,06",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-higher-concentration-of-balsticidin-for-cell-selection'>Determining the Increased Concentration of Balsticidin for Cell Selection</a>",
"text": "

DH5α&BL21

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Blasticidin_testing_III3.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "Strains of HeLa Cells"
       }
     },
     {
       "startDate": "2014,8,26",
       "endDate": "2014,8,26",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Struggles I for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",
"text": "

DH5α&BL21

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-%26-7X-UAS-mCherry-pX330-1.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,27",
       "endDate": "2014,8,27",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-MU2-and-Struggles-II-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Plasmid purification for MU2 and Struggles II for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",
"text": "

DH5α&BL21

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL-1.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,28",
       "endDate": "2014,8,28",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL'>Plasmid purification for Struggle I and Struggle II and Verification with AgeI EcoRI and BbsI for MU2 BIG and SMALL</a>",
"text": "

DH5α&BL21

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL-1.jpg",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,8,29",
       "endDate": "2014,8,29",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-no-endotoxin-for-BIG-and-SMALL'>Plasmid purification no endotoxin for BIG and SMALL</a>",
"text": "

DH5α&BL21

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,30",
       "endDate": "2014,8,30",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_replaceable_0X_2X_5X_7X_UAS-mCherry-pX330_plasmids'>Construction of gRNA replaceable 0X/2X/5X/7X-UAS-mCherry-pX330 plasmids</a>",
"text": "

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Construction-of-gRNA-replaceable-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,31",
       "endDate": "2014,8,31",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI'>Plasmid purification for 0X,2X,5X,7X-UAS-mCherry-pX330 plasmids and Verification with AgeI and BbsI</a>",
"text": "

DH5α&BL21

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,10,6",
       "endDate": "2014,10,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_for_HIV_or_HBV_carried_plasmids'>Construction of plasmids carrying the gRNA for HIV/HBV</a>",
"text": "

DH5α&BL21

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-Construction-of-gRNA-for-HIV-or-HBV-carried-plasmids.jpg",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,22",
       "endDate": "2014,10,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Parts_building'>Building Necessary Parts</a>",
"text": "

We build everything.

", 
       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "2014_SUSTC-Shenzhen_gel_result_0930.2.png",
         "thumbnail": "",
         "credit": "Rifei Chen",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,9,8",
       "endDate": "2014,10,16",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Biobricks_Characterization'>Characterization of Biobricks</a>",
"text": "

Digging deeper into the nature of biology.

", 
       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen_Characterization_9.28_Gel.jpg",
         "thumbnail": "",
         "credit": "Peng Peng",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,13",
       "endDate": "2014,8,19",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/mCherry-BbsI-pointmutation'>mCherry BbsI point mutation</a>",
"text": "

one step for constructing a universal usable gRNA-insertion plasmid.

", 
       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-mCherry-BbsI-pointmutation-first-PCR-result.jpg",
         "thumbnail": "",
         "credit": "Yicong Tao",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,9,29",
       "endDate": "2014,10,7",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Investigation-Cas9-protein-affect-cell-growth'>Investigation Cas9 protein affect cell growth</a>",
"text": "

Investigation of the effect of Cas9 protein on the cell growth(The safety of Cas9).

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "Figure_1._The_cell_grow_curve.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "The Cell Growth Curve"
       }
     },
     {
       "startDate": "2014,9,13",
       "endDate": "2014,9,21",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/G1_cells_transfected_with_px330'>Test of the function of Cas9</a>",
"text": "

Ensure the function of modified px330 targeting EGFP gene.

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-G1_cells_without_pX330_transfection.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "G1 Cells without pX330"
       }
     },
     {
       "startDate": "2014,9,13",
       "endDate": "2014,9,24",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Test-of-the-clone-G3_6'>Test of the first clone of G3 (G3-6)</a>",
"text": "

Prepare for transfection with A-B toxin.

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTCSUSTC-Shenzhen-EGFP-mCherry-fluorescence-G3-6-microscope.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "G3 under Fluorescence Microscope"
       }
     },
     {
       "startDate": "2014,9,20",
       "endDate": "2014,10,7",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/The_quality_test_of_the_clones_of_G3%26G4'>The quality test of the clones of G3&G4</a>",
"text": "

Test the quality of clones we pick from G3 and G4.

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-monoclone-quality-G3-G4-negative-ctrl.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "Normal HeLa Cells"
       }
     },
     {
       "startDate": "2014,9,14",
       "endDate": "2014,9,25",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Examination-of-the-mCherry-expression-of-UAS_mCherry_gRNA'>Examination of the mCherry expression of the plasmids UAS-mCherry-gRNA</a>",
"text": "

Test the quality of UAS-mCherry-gRNA.

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-UAS_test_47hours_transfect_cells.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "Different Periods of G3 under FM"
       }
     },
     {
       "startDate": "2014,10,3",
       "endDate": "2014,10,18",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/A-B-Toxin-transfect'>Transfection of cells with A-B toxin</a>",
"text": "

Introduce gRNA with TEG/GD5 protein.

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-UAS_test_47hours_transfect_cells.png",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "3 Days after Transfection"
       }
     },
     {
       "startDate": "2014,8,16",
       "endDate": "2014,10,16",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify'>Mainstream A-B Toxin Preparation</a>",
"text": "

Purify the Protein and Meanwhile Improve the Protocol.

", 
       "tag": "A-B Toxin",
       "classname": "",
       "asset": {
         "media": "SUSTC-Shenzhen-PWF47-TEG%28coding_sequence%29.jpg",
         "thumbnail": "",
         "credit": "Longze Su",
         "caption": "Coding Sequence"
       }
     },
     {
       "startDate": "2014,8,8",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLa_Cell/blasticidin_screening'>Blasticidin Screening</a>",
"text": "

To test the resistance of HeLa cell to blasticidin.

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "Spread.jpg",
         "thumbnail": "",
         "credit": "Longze Su",
         "caption": "3 Days after Transfection"
       }
     },
     {
       "startDate": "2014,6,12",
       "endDate": "2014,7,27",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Preparation_work'>Experiment Training & Plasmid Building</a>",
"text": "

Introduce gRNA with TEG/GD5 protein.

", 
       "tag": "Fundamental",
       "classname": "",
       "asset": {
       }
     },
     {
       "startDate": "2014,9,22",
       "endDate": "2014,10,4",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/insert-target-to-pBX-083'>PCR & Insert target-sequence to pBX-083</a>",
"text": "

", 
       "tag": "CRISPR",
       "classname": "",
       "asset": {
       }
     },
     {
       "startDate": "2014,8,27",
       "endDate": "2014,8,27",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-blasticidin-for-cell-selection'>Determining the appropriate concentration of blasticidin for cell selection</a>",
"text": "

", 
       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
       }
     }
   ],
   "era": [
     {
       "startDate": "2014,7,27",
       "endDate": "2014,8.7",
       "headline": "Basic Research",
"text": "

The Fundamentals

", 
       "tag": "Fundamental"
     }
   ]
 }

}

Retrieved from "http://2014.igem.org/Team:SUSTC-Shenzhen/Notebook/timeline.json"