Team:SUSTC-Shenzhen/Notebook/timeline.json

From 2014.igem.org

(Difference between revisions)

Latest revision as of 03:48, 18 October 2014

{

 "timeline": {
   "headline": "Lab Notes",
   "type": "default",

"text": "

The steps to success.

",

   "asset": {
     "media": "",
     "credit": "iGEM SUSTC-Shenzhen",
     "caption": "Lab Notes"
   },
   "date": [
     {
       "startDate": "2014,7,27",
       "endDate": "2014,7,28",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Fundamental/TransEfficiency.htm'>Effiency Test</a>",

"text": "

DH5α&BL21

",

       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,8,17",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-puromycin-for-cell-selection'>Determining the appropriate concentration of puromycin for cell selection</a>",

"text": "

Prepare for selection

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lidan Zeng",
         "caption": "Our cells!"
       }
     },
     {
       "startDate": "2014,7,18",
       "endDate": "2014,7,18",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-II-for-plasmid-pX330-EGFP'>Plasmid amplification II for pX330 & EGFP & verifications by electrophoresis</a>",

"text": "

Prepare for selection

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Electrophoresis Snapshot"
       }
     },
     {
       "startDate": "2014,8,9",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Enzyme-digestion-agrose-gel-DNA-Extraction-for-PX330-vector'>Enzyme digestion & agrose gel DNA Extraction for PX330 vector</a>",

"text": "

To prepare PX330 vector for the following ligation experiments

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,6",
       "endDate": "2014,8,6",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/5*UAS-plasmids-Fill-in'>5*UAS plasmids Fill-in</a>",

"text": "

To eliminate the EcoRI site on the 5*UAS plasmid

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,10",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/PCR-for-mCherry'>PCR for mCherry</a>",

"text": "

To get our target fragment of mCherry by PCR

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,9",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-second-time'>UAS Fill-in (The second\ntime)</a>",

"text": "

The same as the first Fill-in experiment

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,11",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Struggles-for-our-second-time-Fill-in'>Struggles for our second-time Fill-in</a>\n",

"text": "

A real challenge, indeed.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,11",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-plasmid-PX330-MCherry-GAL4-PX330-MCherry-amplified-2014-8-9-by-restriction-digestion'>Verification for plasmid PX330-MCherry-GAL4,PX330-MCherry (amplified on 2014.8.9) by restriction digestion</a>",

"text": "

Failure, as it is.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,12",
       "endDate": "2014,8,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-verification-of-plasmid-PX330-MCherry-GAL4'>Follow-up for verification of plasmid PX330-MCherry-GAL4 and The results of 'Struggles for fill-in(the second time)'</a>",

"text": "

Life full of cycles, but each iteration with happiness.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Eluted DNA"
       }
     },
     {
       "startDate": "2014,8,12",
       "endDate": "2014,8,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Follow-up-for-Struggles-for-fill-in-the-second-time'>Follow-up for 'struggles for our second-time Fill-in'</a>",

"text": "

Failure, again.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,13",
       "endDate": "2014,8,16",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/UAS-Fill-in-The-third-time'>UAS Fill-in (The-third-time)</a>",

"text": "

Then try for another time.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "Plates without Colonies"
       }
     },
     {
       "startDate": "2014,8,14",
       "endDate": "2014,8,14",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verifications-for-struggles-for-our-second-time-Fill-in'>Verifications for 'struggles for our second-time Fill-in'</a>",

"text": "

Results verified.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,18",
       "endDate": "2014,8,20",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Successful-fill-in-plasmid-(G56-1)-amplification'>Successful fill-in plasmid (G56-1) amplification</a>",

"text": "

Days of success.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,20",
       "endDate": "2014,8,20",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-UAS'>Point-mutation PCR for UAS</a>",

"text": "

One approach.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,21",
       "endDate": "2014,8,21",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Point-mutation-PCR-for-mCherry-with-BbsI-site-mutation'>Point-mutation PCR for mCherry (with BbsI site mutation)</a>",

"text": "

To eliminate the BbsI site on mCherry fragment.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel under UV Light"
       }
     },
     {
       "startDate": "2014,8,21",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Endotoxin-Free-plasmid-purification-for-pX330-mCherry-pX330-mCherry-GAL4'>Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4</a>",

"text": "

To prepare for further cell culturing.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,22",
       "endDate": "2014,8,22",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Verification-for-pX330-mCherry-pX330-mCherry-GAL4-enzyme-digestion'>Verification for pX330+mCherry, pX330+mCherry+GAL4 by enzyme digestion</a>",

"text": "

To ensure that we have not made errors.

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,25",
       "endDate": "2014,8,26",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Construction-5X-UAS-mCherry-pX330-7X-UAS-mCherry-pX330-I'>Construction of 5X-UAS-mCherry-pX330 & 7X-UAS-mCherry-pX330 I</a>",

"text": "

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,7,17",
       "endDate": "2014,7,19",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/Plasmid-amplification-I-for-plasmid-pX330-EGFP'>Plasmid amplification I for plasmid pX330 & EGFP</a>",

"text": "

Prepare the necessary materials

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,04",
       "endDate": "2014,8,05",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Thawing-HeLa-Cell'>Thawing HeLa Cell</a>",

"text": "

DH5α&BL21

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,14",
       "endDate": "2014,9,20",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Construct-Cellline'>Constructing Cell Line</a>",

"text": "

DH5α&BL21

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "G1 pool cells on petri dish"
       }
     },
     {
       "startDate": "2014,7,27",
       "endDate": "2014,8,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/PB5-TetOn-Cas9-PB3-plasmid-construction'>PB5-TetOn-Cas9-PB3 Plasmid Construction</a>",

"text": "

DH5α&BL21

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,9,27",
       "endDate": "2014,10,06",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-higher-concentration-of-balsticidin-for-cell-selection'>Determining the Increased Concentration of Balsticidin for Cell Selection</a>",

"text": "

DH5α&BL21

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "Strains of HeLa Cells"
       }
     },
     {
       "startDate": "2014,8,26",
       "endDate": "2014,8,26",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Struggles-I-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Struggles I for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",

"text": "

DH5α&BL21

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,27",
       "endDate": "2014,8,27",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-MU2-and-Struggles-II-for-the-first-time-construction-of-5X-UAS-mCherry-pX330-and-7X-UAS-mCherry-pX330'>Plasmid purification for MU2 and Struggles II for the first time construction of 5X-UAS-mCherry-pX330 and 7X-UAS-mCherry-pX330</a>",

"text": "

DH5α&BL21

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,28",
       "endDate": "2014,8,28",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-Struggle-I-and-Struggle-II-and-Verification-with-AgeI-EcoRI-and-BbsI-for-MU2-BIG-and-SMALL'>Plasmid purification for Struggle I and Struggle II and Verification with AgeI EcoRI and BbsI for MU2 BIG and SMALL</a>",

"text": "

DH5α&BL21

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "ProfFan",
         "caption": "Figure 1"
       }
     },
     {
       "startDate": "2014,8,29",
       "endDate": "2014,8,29",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-no-endotoxin-for-BIG-and-SMALL'>Plasmid purification no endotoxin for BIG and SMALL</a>",

"text": "

DH5α&BL21

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {}
     },
     {
       "startDate": "2014,8,30",
       "endDate": "2014,8,30",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_replaceable_0X_2X_5X_7X_UAS-mCherry-pX330_plasmids'>Construction of gRNA replaceable 0X/2X/5X/7X-UAS-mCherry-pX330 plasmids</a>",

"text": "

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,31",
       "endDate": "2014,8,31",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Plasmid-purification-for-0X-2X-5X-7X-UAS-mCherry-pX330-plasmids-and-Verification-with-AgeI-and-BbsI'>Plasmid purification for 0X,2X,5X,7X-UAS-mCherry-pX330 plasmids and Verification with AgeI and BbsI</a>",

"text": "

DH5α&BL21

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,10,6",
       "endDate": "2014,10,10",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Construction_of_gRNA_for_HIV_or_HBV_carried_plasmids'>Construction of plasmids carrying the gRNA for HIV/HBV</a>",

"text": "

DH5α&BL21

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Mengqi Xu, Sijia Liu",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,22",
       "endDate": "2014,10,12",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Parts_building'>Building Necessary Parts</a>",

"text": "

We build everything.

",

       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Rifei Chen",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,9,8",
       "endDate": "2014,10,16",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Biobricks_Characterization'>Characterization of Biobricks</a>",

"text": "

Digging deeper into the nature of biology.

",

       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Peng Peng",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,8,13",
       "endDate": "2014,8,19",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/mCherry-BbsI-pointmutation'>mCherry BbsI point mutation</a>",

"text": "

one step for constructing a universal usable gRNA-insertion plasmid.

",

       "tag": "Fundamental",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Yicong Tao",
         "caption": "DNA Gel"
       }
     },
     {
       "startDate": "2014,9,29",
       "endDate": "2014,10,7",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Investigation-Cas9-protein-affect-cell-growth'>Investigation Cas9 protein affect cell growth</a>",

"text": "

Investigation of the effect of Cas9 protein on the cell growth(The safety of Cas9).

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "The Cell Growth Curve"
       }
     },
     {
       "startDate": "2014,9,13",
       "endDate": "2014,9,21",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/G1_cells_transfected_with_px330'>Test of the function of Cas9</a>",

"text": "

Ensure the function of modified px330 targeting EGFP gene.

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "G1 Cells without pX330"
       }
     },
     {
       "startDate": "2014,9,13",
       "endDate": "2014,9,24",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Test-of-the-clone-G3_6'>Test of the first clone of G3 (G3-6)</a>",

"text": "

Prepare for transfection with A-B toxin.

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "G3 under Fluorescence Microscope"
       }
     },
     {
       "startDate": "2014,9,20",
       "endDate": "2014,10,7",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/The_quality_test_of_the_clones_of_G3%26G4'>The quality test of the clones of G3&G4</a>",

"text": "

Test the quality of clones we pick from G3 and G4.

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "Normal HeLa Cells"
       }
     },
     {
       "startDate": "2014,9,14",
       "endDate": "2014,9,25",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Examination-of-the-mCherry-expression-of-UAS_mCherry_gRNA'>Examination of the mCherry expression of the plasmids UAS-mCherry-gRNA</a>",

"text": "

Test the quality of UAS-mCherry-gRNA.

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "Different Periods of G3 under FM"
       }
     },
     {
       "startDate": "2014,10,3",
       "endDate": "2014,10,18",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/A-B-Toxin-transfect'>Transfection of cells with A-B toxin</a>",

"text": "

Introduce gRNA with TEG/GD5 protein.

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Lin Le",
         "caption": "3 Days after Transfection"
       }
     },
     {
       "startDate": "2014,8,16",
       "endDate": "2014,10,16",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/A-B_Toxin/Purify'>Mainstream A-B Toxin Preparation</a>",

"text": "

Purify the Protein and Meanwhile Improve the Protocol.

",

       "tag": "A-B Toxin",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Longze Su",
         "caption": "Coding Sequence"
       }
     },
     {
       "startDate": "2014,8,8",
       "endDate": "2014,8,11",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLa_Cell/blasticidin_screening'>Blasticidin Screening</a>",

"text": "

To test the resistance of HeLa cell to blasticidin.

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
         "media": "",
         "thumbnail": "",
         "credit": "Longze Su",
         "caption": "3 Days after Transfection"
       }
     },
     {
       "startDate": "2014,6,12",
       "endDate": "2014,7,27",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/Preparation_work'>Experiment Training & Plasmid Building</a>",

"text": "

Introduce gRNA with TEG/GD5 protein.

",

       "tag": "Fundamental",
       "classname": "",
       "asset": {
       }
     },
     {
       "startDate": "2014,9,22",
       "endDate": "2014,10,4",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/CRISPR/insert-target-to-pBX-083'>PCR & Insert target-sequence to pBX-083</a>",

"text": "

",

       "tag": "CRISPR",
       "classname": "",
       "asset": {
       }
     },
     {
       "startDate": "2014,8,27",
       "endDate": "2014,8,27",
       "headline": "<a href='/Team:SUSTC-Shenzhen/Notebook/HeLaCell/Determining-the-appropriate-concentration-of-blasticidin-for-cell-selection'>Determining the appropriate concentration of blasticidin for cell selection</a>",

"text": "

",

       "tag": "HeLa Cell",
       "classname": "",
       "asset": {
       }
     }
   ],
   "era": [
     {
       "startDate": "2014,7,27",
       "endDate": "2014,8.7",
       "headline": "Basic Research",

"text": "

The Fundamentals

",

       "tag": "Fundamental"
     }
   ]
 }

}