Team:Oxford/modellingA

From 2014.igem.org

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<h1>Part A</h1>
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<h2>[DCM] solution-vapour equilibration</h2>
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<h1>[DCM] calibration for solution-vapour equilibration</h1>
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<p>Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data<font style="vertical-align: super; font-size: 70%;"><a href="https://2014.igem.org/Team:Oxford/references">1</a></font>, confirming negligible [DCM] deviation over the concentration and temperature range used and the validity of taking [DCM] = [DCM] unadjusted.</p></div>
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<p>Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data<sup>1</sup>, confirming negligible [DCM] deviation:</p>
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[[File:TDoxigem1.png|centre]]
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<img src="http://latex.codecogs.com/gif.latex?p_{i}=c*k^{\Theta&space;}e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" title="p_{i}=c*k^{\Theta }e^{\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}(\frac{1}{T}-\frac{1}{298.15K})}" /><br>
 
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<img src="http://latex.codecogs.com/gif.latex?k^\Theta=3.9*10^{-6}&space;\:&space;mol&space;\:&space;kg^{-1}&space;Pa^{-1},&space;\frac{\mathrm{d}&space;lnk}{\mathrm{d}&space;1/T}=&space;3900&space;\:K" title="k^\Theta=3.9*10^{-6} \: mol \: kg^{-1} Pa^{-1}, \frac{\mathrm{d} lnk}{\mathrm{d} 1/T}= 3900 \:K" />
 
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Revision as of 14:13, 21 July 2014

Part A

[DCM] solution-vapour equilibration

Testing the tolerance of E-coli and Pseudomonas strains to various concentrations of DCM (0mM, 5mM, 10mM, 20mM), the 15 mL falcon tube-contained system is modeled as a real binary solution/ideal gas, based on literature solubility data1, confirming negligible [DCM] deviation over the concentration and temperature range used and the validity of taking [DCM] = [DCM] unadjusted.

TDoxigem1.png

V(DCM) added (± 5E-4) /ml

n(DCM)(l) unadjusted (± 8E-6) /mol

[DCM] unadjusted (± 2E-3) /M

p(DCM) 

(± 4E-2) /kPa

n(DCM)(g)

(± 4E-7) /mol

n(DCM)(l) (± 9E-6) adjusted /mol

[DCM] adjusted 

(± 2E-3) /M

Δ[DCM] /M

[DCM] % deviation

(30°C, 5mM)

1.6E-03

2.5E-05

5.0E-03

6.2E-01

1.6E-08

2.5E-05

5.0E-03

3.1E-06

6.3E-02

(30°C, 10mM)

3.2E-03

5.0E-05

1.0E-02

3.1E-01

7.9E-09

5.0E-05

1.0E-02

1.6E-06

1.6E-02

(30°C, 20mM)

6.4E-03

1.0E-04

2.0E-02

1.6E-01

3.9E-09

1.0E-04

2.0E-02

7.9E-07

3.9E-03











(37°C, 5mM)

1.6E-03

2.5E-05

5.0E-03

6.2E-01

1.5E-08

2.5E-05

5.0E-03

3.1E-06

6.2E-02

(37°C, 10mM)

3.2E-03

5.0E-05

1.0E-02

3.1E-01

7.7E-09

5.0E-05

1.0E-02

1.5E-06

1.5E-02

(37°C, 20mM)

6.4E-03

1.0E-04

2.0E-02

1.6E-01

3.8E-09

1.0E-04

2.0E-02

7.7E-07

3.9E-03


More detailed system modelling is therefore unnecessary.

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