Team:Macquarie Australia

From 2014.igem.org

(Difference between revisions)
 
(140 intermediate revisions not shown)
Line 3: Line 3:
<head>
<head>
-
<link href="https://2014.igem.org/Template:Team:Macquarie_Australia/css/global?action=raw&ctype=text/css" rel="stylesheet">
+
<link href="https://2014.igem.org/Template:Team:Macquarie_Australia/css/global?action=raw&ctype=text/css" rel="stylesheet">
-
<script>
+
<script src="https://2014.igem.org/Team:Macquarie_Australia/js/banner?action=raw&ctype=text/javascript" type="text/javascript""></script>
-
$(document).ready(function() {
+
</head>
-
//Display slider for Javascript users
+
<body>
-
$('#Slider').css('display', 'block');
+
<section id="HomePageContent">
-
+
<div id="Slider">
-
//Set height of slider (16/5 ratio)
+
<div id="Slide0" class="Slides current"></div>
-
$('#Slider').css('height', ($('body').width() / 3.2));
+
<div id="Slide1" class="Slides"></div>
-
+
<div id="Slide2" class="Slides"></div>
-
//Position right/left arrows
+
<div id="Slide4" class="Slides"></div>
-
var BarHeight = $('#Slider').height() -  $('#BottomLinks').height();
+
<div id="Slide3" class="Slides"></div>
-
$('#LeftBar, #RightBar').css('height', BarHeight);
+
<div id="LeftBar" class="SideBar"><div id="LeftArrow"></div></div>
-
$('#LeftArrow, #RightArrow').css('top', (BarHeight-60)/2);
+
<div id="RightBar" class="SideBar"><div id="RightArrow"></div></div>
 +
<ul id="BottomLinks">
 +
<li id="Link0" class="SlideLink">Overview</li>
 +
<li id="Link1" class="SlideLink">Idea</li>
 +
<li id="Link2" class="SlideLink">Situation</li>
 +
<li id="Link4" class="SlideLink">Outcome</li>
 +
<li id="Link3" class="SlideLink">Future</li>
 +
</ul>
 +
</div>
-
//Repeats for window resize
+
<section id="Overview">
-
$(window).resize(function() {
+
-
$('#Slider').css('height', ($('body').width() / 3.2));
+
-
+
-
var BarHeight = $('#Slider').height() -  $('#BottomLinks').height();
+
-
$('#LeftBar, #RightBar').css('height', BarHeight);
+
-
$('#LeftArrow, #RightArrow').css('top', (BarHeight-60)/2);
+
-
});
+
-
+
-
var TotalSlides = $('.Slides').length;
+
-
var CurrentSlide = 0;
+
-
$('#RightArrow').click(function() {
+
<h1>Our project</h1>
-
CurrentSlide = ++CurrentSlide % TotalSlides;
+
<h2 style="text-align: center;">Watch this cool video below!</h2>
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
$('#LeftArrow').click(function() {
+
-
CurrentSlide = (CurrentSlide + TotalSlides - 1) % TotalSlides;
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
$('.SlideLink').click(function() {
+
-
var ClickedSlide = $(this).attr('id');
+
-
CurrentSlide = parseInt(ClickedSlide.replace('Link', ''));
+
-
changeSlide(CurrentSlide);
+
-
});
+
-
+
-
function changeSlide(num){
+
-
var SlideID = '#Slide' + num;
+
-
$('.Slides').css('display', 'none');
+
-
$(SlideID).css('display', 'block');
+
-
}
+
-
});
+
-
</script>
+
-
</head>
+
-
<body>
+
-
<section id="HomePageContent">
+
-
<div id="Slider">
+
-
<div id="Slide0" class="Slides current"></div>
+
-
<div id="Slide1" class="Slides"></div>
+
-
<div id="Slide2" class="Slides"></div>
+
-
<div id="Slide3" class="Slides"></div>
+
-
<div id="Slide4" class="Slides"></div>
+
-
<div id="Slide5" class="Slides"></div>
+
-
<div id="LeftBar" class="SideBar"><div id="LeftArrow"></div></div>
+
-
<div id="RightBar" class="SideBar"><div id="RightArrow"></div></div>
+
-
<ul id="BottomLinks">
+
-
<li id="Link0" class="SlideLink">Intro</li><li id="Link1" class="SlideLink">Problem</li><li id="Link2" class="SlideLink">Situation</li><li id="Link3" class="SlideLink">Idea</li><li id="Link4" class="SlideLink">Solution</li><li id="Link5" class="SlideLink">Platform</li>
+
-
</ul>
+
-
</div>
+
 +
<html>
 +
<video class='center' poster="https://static.igem.org/mediawiki/2014/e/e4/Video.png" controls>
 +
<source src="https://static.igem.org/mediawiki/2014/3/3d/Prezi_Movie_3.mp4" type='video/mp4'/>
 +
</video>
 +
</html><html>
 +
<br/><br/>
-
<section id="Overview">
+
<h1></h1>
-
<h2>Our Project</h2>
+
-
<p>
+
-
Chlorophyll is a core component in the process of photosynthesis. As a pigment, it harvests light and plays a primary role in the excitation transfer of energy (Eichwurzel, Stiel et al. 2000), which is vital for plant reproduction and survival (Uliana, Pires et al. 2014). The chlorophyll biochemical pathway is an oxygenic photosynthetic process that oxidizes water to produce hydrogen ions. Thirteen genes govern the five-step pathway and each has a specific role.
+
-
</p>
+
-
<p>
+
<h3>Overview</h3>
-
The 2014 Macquarie University iGEM team is continuing the work of the 2013 team to synthetically construct the chlorophyll biochemical pathway in E.coli using synthetic Biobricks from Chlamydomonas reinhardtii. The Biobricks from 2013 have been made using Gibson Assembly. Our aim for 2014 is to improve the Biobricks using synthetic techniques, which will be assembled into three functional operons and expressed in E.coli competent cells.
+
-
</p>
+
-
<p>
+
<p>This project has demonstrated functionality of our designed operons that represent the first step of the chlorophyll a biosynthesis. This was performed through the initial assembly of three operons containing the essential biosynthetic genes that were confirmed through gel electrophoresis and DNA sequencing (Fig. 1).  The functionality of the first operon (Mg-chelatase) was demonstrated through the spectral analysis of its enzymatic product, Mg-Protophoryin IX (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Project/Results">Results</a> page). </p><br/>
-
<img src="https://static.igem.org/mediawiki/2014/7/7a/PathwayMQ.jpg">
+
-
</p>
+
-
</section>
+
<img id="PathwayImg" src="https://static.igem.org/mediawiki/2014/0/08/Homepic1.png" />
-
+
<p><b>Figure 1.</b> Flow chart of the chlorophyll a synthesis pathway. Operons containing the essential genes from <i>Chlamydomonas reinhardtii</i> are represented for their respective steps within the pathway. The spectral change of the compounds are represented in their respective colours of each step.</p>
-
<section id="SectionLinks">
+
 
-
<div class="Wrap">
+
<p>We have also modelled this step of the biosynthetic pathway (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Project/Model">Modelling</a> page). The project was successful in building the foundations for future teams to complete the synthesis of photosystem II in <i>E. coli.</i> This provides a significant leap into the development of a hydrogen-generating bacterial system and a renewable biological energy source. Our policy and practice initiatives were also successful for increasing public awareness of the global energy crisis and the potential synthetic biology has to offer in  providing a solution (which can be seen on the <a href="https://2014.igem.org/Team:Macquarie_Australia/Outreach">Policy & Practice</a> page). </p>
-
<a href="Team:Macquarie_Australia/style/Home">
+
</section>
-
<div id="DescriptionLink1" class="DescriptionLink">
+
       
-
<h2>Lorem ipsum</h2>
+
<section id="SectionLinks">
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
<h2 style="text-align: center;">A huge thank you to our Gold sponsors!</h2>
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
<img src="https://static.igem.org/mediawiki/2014/3/3d/Goldsponsors.png" />
-
</div>
+
<h2 style="text-align: center;">Also many thanks to our Bronze sponsors!</h2>
-
</a>
+
<img src="https://static.igem.org/mediawiki/2014/e/ea/Bronzelogo.png" />
-
+
-
<a href="Team:Macquarie_Australia/style/Home">
+
-
<div id="DescriptionLink2" class="DescriptionLink">
+
-
<h2>Lorem ipsum</h2>
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
-
</div>
+
-
</a>
+
-
<div style="clear:both;"></div>
+
-
<a href="Team:Macquarie_Australia/style/Home">
+
-
<div id="DescriptionLink3" class="DescriptionLink">
+
-
<h2>Lorem ipsum</h2>
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
-
</div>
+
-
</a>
+
-
+
-
<a href="Team:Macquarie_Australia/style/Home">
+
-
<div id="DescriptionLink4" class="DescriptionLink">
+
-
<h2>Lorem ipsum</h2>
+
-
<img src="https://static.igem.org/mediawiki/2013/a/ab/Mqbutton55.png">
+
-
<p> Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.</p>
+
-
</div>
+
-
</a>
+
-
</div>
+
-
</section>
+
-
+
</section>
</section>
-
</body>
+
</section>
-
</html>
+
</body>
 +
</html></html>

Latest revision as of 03:43, 18 October 2014

Our project

Watch this cool video below!



Overview

This project has demonstrated functionality of our designed operons that represent the first step of the chlorophyll a biosynthesis. This was performed through the initial assembly of three operons containing the essential biosynthetic genes that were confirmed through gel electrophoresis and DNA sequencing (Fig. 1). The functionality of the first operon (Mg-chelatase) was demonstrated through the spectral analysis of its enzymatic product, Mg-Protophoryin IX (which can be seen on the Results page).


Figure 1. Flow chart of the chlorophyll a synthesis pathway. Operons containing the essential genes from Chlamydomonas reinhardtii are represented for their respective steps within the pathway. The spectral change of the compounds are represented in their respective colours of each step.

We have also modelled this step of the biosynthetic pathway (which can be seen on the Modelling page). The project was successful in building the foundations for future teams to complete the synthesis of photosystem II in E. coli. This provides a significant leap into the development of a hydrogen-generating bacterial system and a renewable biological energy source. Our policy and practice initiatives were also successful for increasing public awareness of the global energy crisis and the potential synthetic biology has to offer in providing a solution (which can be seen on the Policy & Practice page).

</html>

Retrieved from "http://2014.igem.org/Team:Macquarie_Australia"