Team:Virtus-Parva Mexico/Parts

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<a href="https://2014.igem.org/Team:Virtus-Parva_Mexico"style="text-decoration:none;color:#1C140D"><img src="https://static.igem.org/mediawiki/2014/a/a0/Virtus_Parva_Logo_corto.png" style="width:100px;height:"auto""</img> </a> </td>
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<a href="https://2014.igem.org/Team:Virtus-Parva_Mexico/Project" style="text-decoration:none;color:#1C140D">PROJECT</a> </td>
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<a href="https://2014.igem.org/Team:Virtus-Parva_Mexico/Parts" style="text-decoration:none;color:#1C140D">OUTCOMES</a></td>  
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<a href="https://2014.igem.org/Team:Virtus-Parva_Mexico/Team" style="text-decoration:none;color:#1C140D">THE CREW </a> </td>
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<a href="https://2014.igem.org/Team:Virtus-Parva_Mexico/Notebook" style="text-decoration:none;color:#1C140D">NOTEBOOK </a> </td>
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<a href="https://2014.igem.org/Team:Virtus-Parva_Mexico/Safety" style="text-decoration:none;color:#1C140D">SAFETY </a></td>
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<p align="right"><font color=#FFFFFF size=7><b>Human Practices</b></font>
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                    <h1>HUMAN PRACTICES
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                         <p class="lead"><i>"Science is not made for ourselves" (Niels Bohr in <i>Copenhage</i>)</i>  
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                         <p align="right"><i><font color=#FFFFFF size= 5>"You know how strongly I believe that we don't do science for ourselves, that we do it so we can explain to others..." (Niels Bohr to Heisenberg in <i>'Copenhagen'</i>) </font>
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                   <h2><p>For human practices we have done a variety of things, mostly dedicated to the dissemination of science within our University, locally in the city of Puebla and nationally through collaborations with other high schools</p></2>
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                   <h2><p align="justify"><font color="#ffffff">For human practices we have done a variety of things, mostly dedicated to the dissemination of science within our University, locally in the city of Puebla and nationally through collaborations with other high schools</p></font></h2><br>
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                                 Virtus-Parva
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During the length of our project, we found that importing chemicals and biological agents that we needed were difficult to pass through customs in Mexico. In coordination with other Mexican teams, specially [https://2014.igem.org/Team:Tec-Monterrey/ITESM14_human_practice.html#tab_law-proposal "Tec-Monterrey team"] , we decided to propose to the senate a change in customs law, to make it easier for future iGEMers or other scientists to continue with their investigations.
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of Nanotechnology students who aim to create a better world through love,sympathy and endearment… and through the design and development of a novel technology based on micrometric “drills” to attack pathogen agents.
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<h1 id="sec1"><font color="#666666">Law Initiative</h1></font>
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<font size=4>During the length of our project, we found that importing chemicals reagents and biological agents that we needed were difficult to pass through customs. In coordination with other Mexican teams, specially <a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_human_practice.html#tab_law-proposal"><b>Tec-Monterrey Team</b></a> we propose to a local <i>Deputy Patricia Leal Islas</i> a change in customs law, to make it easier for future iGEMers and other scientists to continue with their investigations. Trough this Virtus-Parva shows as a team it's compromise to help the development of science in Mexico.</>
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First and foremost, we studied different procedures in order to synthesize the strongest and smallest magnetite particles we could make. Magnetite is a molecule that derives from iron, and has observable magnetic properties. Because we wanted to put together magnetite and DNA, we had to make them compatible, task we accomplished by functionalizing it with amino groups, that would allow it to form peptide bonds with our protein, HU. At the same time, our biology team was busy extracting, purifying and transforming E. Coli DNA to work with. This protein, HU, is a histone-like protein normally aids DNA into supercoiling around histones; the “Magnetic-Protein” complex we created mimics the nucleosome in DNA supercoiling process. This allows us to have a DNA “chromatin” with a magnetic core.
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The proposal consists in the reformulation of the classification of biological agents and biomaterials, such as DNA, enzymes and bacteria. We recommended to the authority to employ experts in biology for a better and efficient process in customs</font></p>   
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Combining magnetite, DNA and HU protein, we are building magnetic-core machines, which can be controlled through external electrical impulses. Because of the shape of our system, it is possible for it to have linear movement depending on the frequency applied to it, which has the potential to be incorporated into the medical sector as a pathogen-targeted therapy. This was our original idea and module one of our project.  
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As we were transforming our E. coli cells, we noticed it wasn’t as fast and efficient as we had hoped, which is how we came up with module two of the project. Quite simply, we wanted to take advantage of the shape of our system and its mobility thanks to magnetism in order to make a more efficient transformation. We were able to verify our method was more efficient by making cells express GFP and RFP, which can then be quantified with optic instruments.
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                <h2 id="sec2">The Idea</h2>
 
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                    The basis for these “drills” will be NEMS, nano electro-mechanical systems, technology.<br> We took a survey to fellow iGEMers and external people in order to find out how many people knew about the existence of NEMS and if they knew how they worked. Turns out only 36% of survey takers had heard of the term before and of those, only 28% knew what it was!<br><br> Given these statistics, it became part of our project to teach newer generations about our subject.
 
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                                <h3>NEMS</h3>
 
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                                NEMS are nanometric electromechanical systems. In this case we take as basis the structure of a resonator which are engineered to make a conversión between energy, such as electric, magnetic, or vibrational into mechanical response.
 
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How exactly do NEMS come into play in our project?
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Well, by combining an inorganically synthesized nanoparticle, called magnetite and DNA into what we call BioNEMS drill.
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                                <h3>DNA Coiling into Chromosomes</h3>
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                            <p align="justify"><font size=4> Of course we wanted information about science to reach as far and wide as we possibly could, beyond the small talk we gave before our movie projection. Because of this, we formed an alliance with a couple of art majors and we decided to stage the play “Copenhaguen”. This play stages the visit of German physicist and Nobel laureate Werner Heisenberg into Copenhaguen to visit his friend, Niels Bohr; conflict arises when, after that mysterious visit, both colleagues rupture their 20 year society, and a pivoting point during the Second Great War takes place. The staging of the play was to be intertwined with informative capsules on the topics of the play. </font></p>
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                <h2 id="sec3">The Making</h2>
 
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                    Descripcion general de Seccion 3
 
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                                The first part of the synthesis of our magnetite was trying out different methods and characterizing them, to note which method had given us the smallest size nanoparticles. Our first method was synthesis by coprecipitation, of which we prepared nine samples with different concentrations of iron(II) chloride and ammonium hydroxide; from this method we consistently obtained nanoparticles rounding 0.9 to 1nm. Our following method was very similar, but included water in the synthesis: the size of our particles would vary greatly, from 3.89 micrometers to 171 nanometers in size.
 
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After choosing the best method possible, it was time to silanize our magnetite in order for it to be biocompatible with DNA and be able to tie them together. In order for the silanization to take place, we used a solution of TEOS (tetraethoxysilane) dispersed in a medium of water and propanol and dripped this mix slowly onto our magnetite. Just like when we synthesized our particles, we tested different concentrations of TEOS and magnetite, as well as different addition rates in order to observe which combination would give us the smallest possible nanoparticles.  
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                    <p align="center"><font color="#ffffff" size="4">We also found that being the first iGEM team from our university was not an easy task, advice from a past iGEMer would have been very helpful. Other teams noticed this as well, in particular, ITESM-CEM Team. In cooperation with them, we decided to write an extensive handbook for future iGEM teams that need to consult on the mechanics of the contest and need a little push to continue teams in their own universities.  
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We have made 3 volumes, named : "Good Laboratory Practices: an iGEM laboratory work handbook", "The iGEM Team Handbook", "Parts Registry Handbook: A Guideline into iGEM's Standardized Parts. Click on the images below to open the desired handbook in another window. <br><br></font></p></td><td width="162px" bgColor="#355079"></td></tr></table>
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Our results were then characterized by DLS (dynamic light scattering), for which we observed a peak at 39 nm, once coated with TEOS, the peak was moved toward 60 and 80 nm. We also ran our two samples in the IR, comparing the spectra of the pure magnetite and silanized magnetite, we were able to distinguish a peak at 990.2 cm^-1 corresponding to a Si-O bond, confirming the correct silanization of the magnetite. 
 
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A big part of dissemination of science is to include young adults in high school, teach them enough about science so as to peak their interest. In accordance to our goals, we decided to give a general, dynamic talk about nanotechnology, bionanotechnology and iGEM in career fairs for people close to graduation.
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<tr><td><p align="justify"><font color="#000000" size="3.5">It is customary in our campus to project a film every Tuesday, run by a club called “Cineclub”. We decided to take advantage of this ongoing tradition to present a film related to synthetic biology and to give a very quick presentation on the basics of synthetic biology. This way we could get a token amount of information for people within our community and allowed them to reach out to us in case anyone wanted more information.</font> </p></td></tr>
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<a href="http://www.luisartphotography.com/"><img src="https://static.igem.org/mediawiki/2014/e/e4/Virtus_Parva-P1.jpg" width=200px/></img></a>
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<a href="https://www.facebook.com/pages/Mesa-Directiva-de-Ciencias-Qu%C3%ADmico-Biol%C3%B3gicas-UDLAP/560508417305601?ref=ts&fref=ts"><img src="https://static.igem.org/mediawiki/2014/e/ef/Virtus_Parva-P2.jpg"/></img></a>
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<a href="http://www.concytep.puebla.gob.mx/"><img src="https://static.igem.org/mediawiki/2014/4/45/Virtus_Parva-P3.jpg" width=200px/></img></a>
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<a href="http://www.udlap.mx/home.aspx"><img src="https://static.igem.org/mediawiki/2014/7/7e/Virtus_Parva-P4.jpg" width=200px /></img></a>
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Latest revision as of 03:42, 18 October 2014



Human Practices

"You know how strongly I believe that we don't do science for ourselves, that we do it so we can explain to others..." (Niels Bohr to Heisenberg in 'Copenhagen')

For human practices we have done a variety of things, mostly dedicated to the dissemination of science within our University, locally in the city of Puebla and nationally through collaborations with other high schools

Law Initiative



During the length of our project, we found that importing chemicals reagents and biological agents that we needed were difficult to pass through customs. In coordination with other Mexican teams, specially Tec-Monterrey Team we propose to a local Deputy Patricia Leal Islas a change in customs law, to make it easier for future iGEMers and other scientists to continue with their investigations. Trough this Virtus-Parva shows as a team it's compromise to help the development of science in Mexico.

The proposal consists in the reformulation of the classification of biological agents and biomaterials, such as DNA, enzymes and bacteria. We recommended to the authority to employ experts in biology for a better and efficient process in customs

 Computer Hope


Copenhagen Play




Of course we wanted information about science to reach as far and wide as we possibly could, beyond the small talk we gave before our movie projection. Because of this, we formed an alliance with a couple of art majors and we decided to stage the play “Copenhaguen”. This play stages the visit of German physicist and Nobel laureate Werner Heisenberg into Copenhaguen to visit his friend, Niels Bohr; conflict arises when, after that mysterious visit, both colleagues rupture their 20 year society, and a pivoting point during the Second Great War takes place. The staging of the play was to be intertwined with informative capsules on the topics of the play.



Manuals



We also found that being the first iGEM team from our university was not an easy task, advice from a past iGEMer would have been very helpful. Other teams noticed this as well, in particular, ITESM-CEM Team. In cooperation with them, we decided to write an extensive handbook for future iGEM teams that need to consult on the mechanics of the contest and need a little push to continue teams in their own universities.

We have made 3 volumes, named : "Good Laboratory Practices: an iGEM laboratory work handbook", "The iGEM Team Handbook", "Parts Registry Handbook: A Guideline into iGEM's Standardized Parts. Click on the images below to open the desired handbook in another window.



Conferences

Computer Hope

A big part of dissemination of science is to include young adults in high school, teach them enough about science so as to peak their interest. In accordance to our goals, we decided to give a general, dynamic talk about nanotechnology, bionanotechnology and iGEM in career fairs for people close to graduation.

Computer Hope


Movie Projection

It is customary in our campus to project a film every Tuesday, run by a club called “Cineclub”. We decided to take advantage of this ongoing tradition to present a film related to synthetic biology and to give a very quick presentation on the basics of synthetic biology. This way we could get a token amount of information for people within our community and allowed them to reach out to us in case anyone wanted more information.

Computer Hope

Retrieved from "http://2014.igem.org/Team:Virtus-Parva_Mexico/Parts"