Team:USyd-Australia

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<tr><td width="30%" align="center"><h3>The Team</h3></td>
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<tr><td colspan="3"><h3>Integrons as a novel cloning system in E. coli</h3></td></tr>
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<tr><td colspan="3">Integrons are systems that can capture and utilise mobile genetic elements known as gene cassetes, and which are found in a vast array of bacteria, often associated with antibiotic resistance. Gene cassettes with an attC recognition site are site selectively recombined into an attI recognition site by the integron integrase. We are seeking to design biobrick compatible integron components that can be used as a novel, convenient and selective cloning system in lab strains of E. coli, which normally do not contain integron systems. Additionally, we are investigating regulation of natural transformation mechanisms in E. coli in an attempt to allow uptake and recombination of attC containing genetic elements with added convenience. This will include investigating the role of the transcription factor sxy, as well as other components of the DNA uptake systems of other gram negative bacteria that are naturally transformable. </td></tr>
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<td align="center"><h3>Integrons: Nature's cloning tool </h3></td>
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<tr><td colspan="3"> <h3> Requirements </h3></td></tr>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<td width="30%" align="center"><h3>Strange Nature</h3></td>
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<a href="https://2014.igem.org/Team:USyd-Australia/Team">
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<img src="https://static.igem.org/mediawiki/2014/3/3c/USyd-Australia_Profile-Abi.jpg" width="125px" vspace="2px">
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<img src="https://static.igem.org/mediawiki/2014/c/c7/USyd-Australia_Profile-Andy.jpg" width="125px" vspace="2px"> <br>
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<li><a href="https://2014.igem.org/Team:USyd-Australia">Home</a> </li>
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<a href="https://2014.igem.org/Team:USyd-Australia/Project/Design">
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<li><a href="https://2014.igem.org/Team:USyd-Australia/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=USyd-Australia">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:USyd-Australia/Project">Project</a> </li>
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<li><a href="https://2014.igem.org/Team:USyd-Australia/Safety">Safety</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>Do not use flash in wiki code. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<tr> <td colspan="3"  height="15px"> </td></tr>
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<tr><td colspan="5 align="center"><h3>Integrons as a novel cloning system in E. coli</h3></td></tr>
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<td colspan="5" align="justify"><a href="https://2014.igem.org/Team:USyd-Australia/Project/Background">Integrons</a> are natural systems for moving genetic material. They are found in a vast array of bacteria, often associated with rapidly evolving traits such as antibiotic resistance. This is because integrons capture and express mobile genetic elements known as gene cassettes, thus facilitating horizontal gene transfer. Gene cassettes are site selectively recombined by the integron integrase. We have <a href="https://2014.igem.org/Team:USyd-Australia/Project/Design">designed</a> BioBrick- compatible integron components that can be used as a novel, convenient and selective cloning system. Additionally, we have been investigating regulation of natural transformation mechanisms in E. coli in an attempt to allow uptake and recombination of attC containing genetic elements with added convenience. </td>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
 
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
 
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
 
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<li> <a href="https://2013.igem.org/Team:Lethbridge">2013 Lethbridge</a></li>
 
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and what you plan to do.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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Latest revision as of 03:41, 18 October 2014

iGEM_Link


The Team

Integrons: Nature's cloning tool

Strange Nature



Integrons as a novel cloning system in E. coli

Integrons are natural systems for moving genetic material. They are found in a vast array of bacteria, often associated with rapidly evolving traits such as antibiotic resistance. This is because integrons capture and express mobile genetic elements known as gene cassettes, thus facilitating horizontal gene transfer. Gene cassettes are site selectively recombined by the integron integrase. We have designed BioBrick- compatible integron components that can be used as a novel, convenient and selective cloning system. Additionally, we have been investigating regulation of natural transformation mechanisms in E. coli in an attempt to allow uptake and recombination of attC containing genetic elements with added convenience.

With thanks to:

Retrieved from "http://2014.igem.org/Team:USyd-Australia"