Team:XMU-China/Notebook Protocol

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Latest revision as of 03:40, 18 October 2014

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Protocols

General Considerations

E. coli Strains

E. coli strain DH5α, was offered by XMU-China iGEM 2013.

E. coli strain CL-1, whose gene of cheZ had been knocked out, was offered generously by iGEMer JianDong Huang and Lizhong Liu, from Department of Biochemistry, The University of Hong Kong.

Antibiotic

Antibiotic	Stock Concentration (mg/mL)	Final Concentration (μg/mL)	Solvent
Chloramphenicol	50	50	Absolute ethyl alcohol
Ampicillin	50	100	ddH2O
Kanamycin	50	50	ddH2O
Tetracycline	50	50	Absolute ethyl alcohol

 All the antibiotics above require filtration to sterilize.

 Stock in -20°C

LB Liquid Medium

Ingredients	Percent (g/mL)
Yeast Extract	0.5%
Tryptone	1%
NaCl	1%

LB Solid Medium

Ingredients	Percent (g/mL)
Yeast Extract	0.5%
Tryptone	1%
NaCl	1%
Agar	1.5%

LB Semi-Solid Medium

Ingredients	Percent (g/mL)
Yeast Extract	0.5%
Tryptone	1%
NaCl	1%
Agar	0.25%

Preparation of Competent Cell (TaKaRa Competent Cell Preparation Kit)

 Activate the E. coli strain on LB-plate from glycerol stock under the condition of 37°C for 12 hours.

 Pre-culture a single colony in 5 mL LB liquid medium under the condition of 37°C, 200 rpm for 12 hours.

 Add 200 μL pre-culture bacteria into 20 mL LB liquid medium. Culture under the condition of 37°C, 200 rpm for 2 hours.

 Place on ice for 30 min.

 Subpackage the 20 mL culture medium into 1.5 mL sterile centrifuge tube, each centrifuge tube containing 1 mL.

 Centrifuge at 1500×g for 5 min at 4°C, then discard supernatant.

 Re-suspend each centrifuge tube gently with 100 μL Solution A, which has been precooled in ice.

 Centrifuge at 1500×g for 5 min at 4°C, then discard supernatant.

 Re-suspend each centrifuge tube gently with 100 μL Solution B, which has been precooled in ice.

 Stock in -20°C

Transformation

 Add 1 μL purified plasmid or 10 μL ligation system into 100 μL fresh competent cells, which is contained in 1.5 mL centrifuge tube. Then mix gently.

 Leave on ice for 30 minutes.

 Heat shock at 42°C for 90 seconds.

 Leave on ice for 10 minutes immediately.

 Add 400 μL fresh LB medium into the centrifuge tube.

 Incubate for 2 hours under the condition of 37°C, 200 rpm.

Note: If the resistance of vector is ampicillin, incubation time is shortened to 1 hour.

 Spread 100 μL culture medium on a LB solid medium plate, which contains appropriate antibiotics.

 Incubate overnight at 37°C

Confirmation

1. Plasmid Extraction (Omega Plasmid Mini Kit)

 Add 1 mL overnight suspension culture into a 1.5 mL sterile centrifuge tube. Centrifuge at 10000 x g for 1 min, then discard supernatant.

 Repeat the step above several times, until the amount of bacteria is appropriate.

 Re-suspend cells gently with 250 μL Solution I/RNase A.

 Add 250 μL Solution II into each centrifuge tube. Mix gently by inverting 4-6 times to obtain cleared lysate.

 Add 350 μL Solution III into each centrifuge tube. Mix well to form protein precipitate.

 Centrifuge at ≥13000 x g for 10 min.

 Transfer cleared lysate into a HiBind Mini Column placed in a 2 mL collection tube.

 Centrifuge at 10000 x g for 1 min. Discard liquid.

 Wash the HiBind Mini Column with 500 μL HB Buffer. Centrifuge at 10000 x g for 1 min. Discard liquid.

 Using the same collection tube, wash the HiBind Mini Column with 700 Μl DNA Wash Buffer, which is diluted with ethanol. Centrifuge at 10000 x g for 1 min. Discard liquid.

 Wash the HiBind Mini Column a second time with an additional 700 μL DNA Wash Buffer. Centrifuge at 10000 x g for 1 min. Discard liquid.

 Centrifuge the empty HiBind Mini Column at ≥ 13000 x g for 2 min to spin-dry.

 Place the HiBind Mini Column into a sterile 1.5 mL centrifuge tube.

 Add 30 μL sterile ultrapure water directly into the HiBind Mini Column. Sit at room temperature for 2 minutes. Centrifuge at maximum speed (≥ 13000 x g) for 1 min to elute DNA.

2. Digestion for Confirmation

Reaction condition: 37°C for 2 hours.

Single Digestion		Double Digestion
Plasmid	5 μL	Plasmid	5 μL
EcoR I	0.5 μL	Xba I	0.5 μL
		Pst I	0.5 μL
10×H Buffer	1 μL	10×M Buffer	1 μL
ddH2O	3.5 μL	ddH2O	3 μL
Total Volume	10 μL	Total Volume	10 μL

3. DNA gel electrophoresis (take 20 mL as example)

 Dilute 50×TAE to 1×TAE with ddH2O.

 Measure 20 mL 1×TAE buffer.

 Pour 1×TAE buffer into an erlenmeyer flask.

 Weigh 0.2g agarose to make 1% DNA gel.

 Pour agarose into the erlenmeyer flask.

 Make agarose melt by microwave, lasting for 105 seconds until melt completely.

 Cool for 5 minutes.

 Assemble gel pouring apparatus by inserting gate into slots.

 Pour agarose gel into gel tray.

 Bring a piece of silver paper on the top of the gel pouring apparatus.

 Wait for 30-40 minutes to solidify the DNA agarose gel.

Ligation

1. Digestion for Ligation

Reaction condition: 37°C for 2.5 hours.

Insert(A) + Vector(B)				Vector (A) + Insert (B)
Insert(A)	30 μL	Vector(B)	30 μL	Insert(B)	30 μL	Vector(A)	30 μL
EcoR I	3 μL	EcoR I	4.5 μL	Xba I	3 μL	Spe I	4.5 μL
Spe I	3 μL	Xba I	4.5 μL	Pst I	3 μL	Pst I	4.5 μL
10×H Buffer	4 μL	10×M Buffer	4 μL	10×M Buffer	4 μL	10×H Buffer	4 μL
ddH2O	0 μL	ddH2O	7 μL	ddH2O	0 μL	ddH2O	7 μL
Total Volume	40 μL	Total Volume	50 μL	Total Volume	40 μL	Total Volume	50 μL

2. Gel Extraction of DNA (Omega Gel Extraction Kit)

 Perform DNA gel electrophoresis to fractionate DNA fragments.

 When adequate separation of bands has occurred, excise the DNA fragments of interest carefully.

 Weigh in sterile 1.5 mL centrifuge tube.

 Add corresponding volume of Binding Buffer (XP2) into the centrifuge tube.

 Heat the DNA/gel mixture at 55-65°C for 7 min, until the gel melt completely.

 Put a HiBind DNA Mini Column in a 2 mL collection tube. Add the DNA/gel solution to the HiBind DNA Mini Column.

 Centrifuge at 10000 x g for 1 min at room temperature. Discard the flow-through liquid.

 Add 300 μL Binding Buffer (XP2) into the centrifuge tube. Centrifuge at 10000 x g for 1 minute at room temperature. Then discard the flow-through liquid.

 Add 700 μL SPW Wash Buffer (diluted with absolute ethanol). Centrifuge at 10000 x g for 1 minute at room temperature. Then discard the flow-through liquid.

 Repeat the previous step.

 Centrifuge the empty HiBind Mini Column at ≥ 13000 x g for 2 min to spin-dry.

 Place the HiBind Mini Column into a sterile 1.5 mL centrifuge tube.

 Add 30 μL Elution Buffer directly into the HiBind Mini Column. Sit at room temperature for 2 minutes. Centrifuge at maximum speed (≥ 13000 x g) for 1 min to elute DNA.

3. Ligation

 Reaction condition: 16°C for 5-12 hours.

 Measure the concentration of DNA fragments and vector which are going to be ligated.

 Calculate the volume of insert fragments and vector which should be added, based on the formula:

 Add DNA fragments, vector, ligation buffer and T4 Ligase together in an EP tube as the table below.

Insert Fragments	V1 μL
Vector	V2 μL
10×Ligation Buffer	1 μL
T4 Ligase	1 μL
Add ddH2O until the total volume equals 10.0 μL

Note: Mixing the ligation system well by pipetting up and down gently is necessary.

General PCR (Polymerase Chain Reaction)

PCR System

Template	1 μL
Forward Primer	2 μL
Reverse Primer	2 μL
dNTP Mixture	2 μL
10×PCR Buffer	5 μL
rTaq (TakaRa)	1 μL
ddH2O	37 μL
Total Volume	50 μL

PCR Condition

1	94°C	5 min
2	94°C	30 s	30 loops
3	Tm°C	30 s
4	72°C	1 min/kb
5	72°C	10 min
6	15°C	5 min

Conic curve formation

Medium: M63 [1] semi-solid medium preparation

M63 semi-solid medium/100 mL
Ingredients	Quantities
KH2PO4	1.36 g
KOH	0.42 g
(NH4)2SO4	0.2 g
MgSO4	0.012 g
Agar( gel strength>750 g/cm2)	0.25 g
H2O 1	96 mL
Glycerol	0.2 mL
FeSO4 2	10.84*10-4 g
D-glucose	0.4 g
Asp 3	6.6*10-6 g
Met, Leu, His, Thr (0.015 g/mL) 4	1 mL

Note：

1. Add 0.25 g Agar into a erlenmeyer flask first, then pour into 96 mL H2O, shaking up, continue to add glycerol using pipette.

2. Add 10 μL 0.1 g/mL FeSO4 solution into the medium.

3. Add 10 μL 66 mg/mL Asp solution into the medium.

4. Met, Leu, His, Thr could be premixed for convenience, and the amino acid should be added by injector through 0.22 μm filter membrane. Pay attention that this process must be carried out after high temperature sterilization of the M63 semi-solid medium.

I. Mix-doting bacteria, inductor and inhibitor on M63 semi-solid medium plate [2]

1. Pour 20 ml M63 semi-solid medium on a plate, leave the plate air-dried for 90 min in laminar flow clean bench.

2. Dot 15 μL inductor (IPTG) or inhibitor (L-Arabinose) on the plate through pipette, take care when insert the tip into the medium. Leave the plate air-dried for 20 min in laminar flow clean bench.

3. Dot 3 μL culture medium at the same position of the inductor (IPTG) or inhibitor (L-Arabinose), leave the plate air-dried for 5 min in laminar flow clean bench.

4. Seal the plate.

II. Lineation of inductor and inhibitor on the M63 semi-solid medium plate

1. Pour 20 mL M63 semi-solid medium on a plate, leave the plate air-dried for 90 min in laminar flow clean bench.

2. Inhale 15 μL inductor (IPTG) or inhibitor (L-Arabinose), doting along the line pre-pianted carefully, bear in mind don’t destroy the semi-solid medium when moving along the line.

3. Leave the plate air-dried for 90 min in laminar flow clean bench.

4. Repeat the process mix-doting bacteria, inductor and inhibitor on M63 semi-solid medium plate.

Characterization the activity of promoter and efficiency of RBS by chemotaxis

culture

1. Add 50 μL bacterium solution (pLac-RBS (1.0)-CheZ-TT, pLac-RBS (0.3)-CheZ-TT, pLac-RBS (0.01)-CheZ-TT,) into 5 mL fresh LB liquid medium, in which chloromycetin concentration is 50 μg/ml.

2. Culture overnight under the condition of 37°C, 200 rpm.

3. Add 50 μL bacterium solution from the LB liquid medium above to another 5 mL fresh LB liquid medium, in which chloromycetin concentration is 50 μg/mL.

4. Culture under the condition of 37°C, 200 rpm for 3 hours. Stock it in 4°C.

5. Prepare M63 semi-solid medium as above.

6. Draw three dots on a M63 semi-solid medium plate first, then inoculate the three kinds of bacterium solution at the three dots, respectively. The volume of the inoculated bacterium solution is 3 μL.

7. Culture in incubator at 37°C.

Measurement

1. Use a ruler to measure the radius of colony from the bottom of the semi-solid medium plate. The initial colony radius is recorded as R1, and the radiuses measured at 12h, 24h, 30h, 36h, 42h... as R2, R3, R4, R5, R6, R7…

2. Record time and diameter in a table.

3. Process data with excel.

Black hole

Plasmid Construction

All DNA parts applied in these experiments came from the MIT Registry of Standard Biological Parts. The new BioBrick part, PompC-cheZ (BBa_K1412010) was formed by ligating the pre-existing parts, PompC (BBa_R0082) and cheZ (BBa_K629003). The E.Z.N.A.TM Plasmid Mini Kit I (200) was used according to the manufacturer’s instructions to extract plasmid DNA. BioBrick parts were digested with restriction enzymes, EcoRI, Xba I, Spe I, and Pst I to produce ‘sticky ends’ that were used to combine BioBrick parts. Digested DNA was purified using 1% agarose gel electrophoresis followed by gel purification with the E.Z.N.A.TM Gel Extraction Kit (200). BioBrick parts with matching ‘sticky ends’ were ligated using T4 ligase produced by Takara Biotechnology (Dalian) Co., Ltd. (Takara Dalian). Plasmid DNA was transformed into DH5α

first. Then we began a new circulation consisting of extracting plasmids, digestion and DNA gel electrophoresis to confirm the success of the ligation. Finally, the construct was sequenced for verification.

Construction of E. coli strain CL-1

The new biobrick part, PompC-cheZ (BBa_K1412010) on pSB1C3 was introduced into E. coli strain CL-1, whose gene of cheZ had been knocked out.

I. Gradient Plate Experiments (semi-solid medium, Cm 50μg/ml)

Medium: Medium A [3] (Improved)

Ingredients	Percent (g/mL)
Yeast Extract	0.26%
Peptone	0.27%
K2HPO4	0.37%
KH2PO4	0.13%
NaCl	0.05%
Glycerol	0.2%

Sucrose concentration gradient: 0~20%, the concentration gradient was 2%.

Mid-log-phase cell suspensions preparation:

1. Overnight culture of the required strain grow at 37 °C in Medium A (Improved) containing appropriate chloramphenicol.

2. Dilute the overnight culture at the ratio of 1:100 in fresh culture (Medium A), then incubate the culture under the condition of 37°C, 200 rpm, until its OD590 reaches 0.2.

3. Stock in 4°C.

The method of preparing gradient semi-solid medium (0.25% agar)

1. Prepare 100% sucrose solution.

2. Prepare the medium with tripled ingredients amount.

3. Put the 100% sucrose solution, the prepared medium and plenty of deionized water into the autoclave for 121°C, 20 minutes.

4. After sterilization, put the 100% sucrose solution, the prepared medium and the deionized water into a drying oven for around 10 minutes.

5. Take 5 mL tripled Medium A (Improved) into a 25 mL erlenmeyer flask, then add appropriate volume of 100% sucrose solution into the erlenmeyer flask, while the shortage will be complemented by deionized water to make the total volume to be 15 mL.

6. Add 15 μL chloramphenicol into the erlenmeyer flask.

7. Pour the mixture into a Petri dish (85 mm dia.) as quickly as possible.

8. Leave the plate air-dried for 90 min in laminar flow clean bench.

9. After solidification of the media, cell suspensions from mid-log-phase cultures (3 μL) were inoculated in the center of the plate.

10. Leave the plate dried in air for 15 min in laminar flow clean bench.

11. Leave the plate incubated at 37°C. During the period of time, measure the diameters of the colony and take them down.

II. Plates Crossed Experiments (semi-solid medium, Cm 50μg/ml)

Medium: half Medium A (Improved)

Ingredients	Percent (g/mL)
Yeast Extract	0.13%
Peptone	0.135%
K2HPO4	0.195%
KH2PO4	0.065%
NaCl	0.025%
Glycerol	0.1%

The concentration of sucrose: 0%, 10%

The method of preparing liquid medium containing different concentrations of sucrose:

1. Prepare the medium with corresponding ingredients amount in the table above.

2. Add corresponding amount of solid sucrose into the liquid medium.

3. Wait for sterilization.

Mid-log-phase cell suspensions preparation:

1. Overnight culture of the required strain grow at 37°C in Medium A (Improved) containing appropriate chloramphenicol.

2. Dilute the overnight culture at the ratio of 1:100 in fresh culture (half Medium A), then incubate the culture under the condition of 37°C, 200 rpm, until its OD590 reaches 0.2.

3. Stock in 4°C.

The method of preparing semi-solid medium with a cross (0.25% agar)

1. Prepare the medium with corresponding ingredients amount in the table above.

2. Put the medium containing agar, the liquid medium containing sucrose and plenty of deionized water into the autoclave for 121°C, 20 minutes.

3. After sterilization, put the mediums and the deionized water into a drying oven for around 10 minutes.

4. Take 15 mL of the medium containing agar into a 25 mL erlenmeyer flask.

5. Add 15 μL chloramphenicol into the erlenmeyer flask.

6. Pour the mixture into a Petri dish (85 mm dia.) as quickly as possible.

7. Leave the plate air-dried for 90 min in laminar flow clean bench.

8. After solidification of the media, draw a cross composed of two straight lines on the plate, by the way of Intensive RBI (1 μL/mm). One straight line is dotted with water, the other is dotted with liquid medium containing 10% sucrose. The length of the lines is 6 centimeters.

9. Leave the plate dried in air for 15 min in laminar flow clean bench.

10. Cell suspensions from mid-log-phase cultures (3 μL) is inoculated at the cross point.

11. Leave the plate dried in air for 15 min in laminar flow clean bench.

12. Leave the plate incubated at 37°C. During the period of time, take pictures of the colony.

Osicllation timer

Medium: Luria–Bertani (LB) medium [4]

Luria–Bertani (LB) medium/100 mL
Ingredients	Quantities
Yeast Extract		0.25 g
Bacto Tryptone		0.5 g
NaCl		0.5 g
Glycerol 1		0.5 g
0.1 M HEPES 2		3-4 mL
NaOH 3		Until pH 8.0

Note :

1. Glycerol is used as the carbon source.

2. 3-4 mL 0.1 M HEPES is added after rich defined medium is utilized, serving for minimizing the effects of pH.

3. NaOH is added until PH of the medium reaches 8.0.

Experiments

1. Pour 10 mL of the medium above containing 0.25% agar (Difco, Bacto agar) into a Petridish to prepare semi-solid medium.

2. Leave the plate air-dried for 90 min in laminar flow clean bench at room temperature.

3. Spot medium culture in half thickness in the centern of the plate.

4. Cultivate at 37°C.

Aptamer key-lock

PCR System

Template	1 μL
Forward Primer	1 μL
Reverse Primer	1 μL
dNTP Mixture	4 μL
10×PCR Buffer	5 μL
rTaq (TakaRa)	1 μL
ddH2O	37 μL
Total Volume	50 μL

The source and formation of the idea

Designation	Sequence
theophylline Aptamer1	GGU GAU ACC AGC AUC GUC UUG AUG CCCUUG GCA GCA CC
theophylline Aptamer_RBS_AUG1	GGU GAU ACC AGC AUC GUC UUG AUG CC CUU GGC AGC ACC CC G CUG CAA GAC AAC AAG AUG
crRNA	CGU CUU GAU UCC UUC UUU
RBS	AGG AGG AAA
taRNA	GCA GAA CUA AGG AAG AAA

Sequence

Designation	Sequence( 5’-3’)	Length
Lock Templates	TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACGAATTT CTTTCTTCCTTAG TTCTGCCTAAGGAGGAAA	91 bp
Forward Primer	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGA	50 bp
Reverse primer	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTCCTCCTTAGGCAGAACT	49 bp
Aptamer key Templates	TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACGAATTTCGGTGAT ACCAGCATCGTCTTGATGCCCTTGGCAGCACCCTGCGTAAAGAAGGAATCAAGACG	123 bp
Forward primer	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGA	53 bp
Reverse primer	GTTTCTTCCTGCAGCGGCCGCTACTAGTACGTCTTGATTCCTTCTTTACGCA	52 bp
New RBS Templates	TCCCTATCAGTGATAGAGATTGACATCCCTATCAGTGATAGAGATACTGAGCACTAC TAGAGAGGAGGAAA	71 bp
Forward primer	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGA	50 bp
Reverse primer	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTCCTCCTCTCTAGTAGTG	49 bp

PCR Conditions

Lock

1	94°C	5 min
2	94°C	30 s	30 loops
3	55°C	30 s
4	72°C	30 s
5	72°C	10 min
6	15°C	5 min

Aptamer Key

1	94°C	5 min
2	94°C	30 s	30 loops
3	52°C	30 s
4	72°C	30 s
5	72°C	10 min
6	15°C	5 min

New RBS

1	94°C	5 min
2	94°C	30 s	30 loops
3	55°C	30 s
4	72°C	30 s
5	72°C	10 min
6	15°C	5 min

References

[1]Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli[J]. Journal of General Microbiology, 1973, 74(1): 77-91.

[2] S T, JP. G. Guiding bacteria with small molecules and RNA[J]. Journal of the American Chemical Society, 2007, (21):6807-6811.

[3] Kawaji, H., T. Mizuno, and S. Mizushima. "Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins O-8 and O-9 of Escherichia coli K-12." Journal of bacteriology 140.3 (1979): 843-847.

[4] Liu C, Fu X, Liu L, et al. Sequential establishment of stripe patterns in an expanding cell population[J]. Science, 2011, 334(6053): 238-241.

[5] Topp S, Gallivan J P. Guiding bacteria with small molecules and RNA[J]. Journal of the American Chemical Society, 2007, 129(21): 6807-6811.

Team:XMU-China/Notebook Protocol

From 2014.igem.org

Latest revision as of 03:40, 18 October 2014

Protocol

Lab notes

Calendar

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      <span style="font-family:Arial;font-weight:700;font-size:21px;">General Considerations</span>
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      <span style="font-family:Arial;font-weight:700;font-style:italic;color:#fff;font-size:16px;">E. coli</span><span style="font-family:Arial;font-weight:700;color:#fff;font-size:16px;"> </span><span style="font-family:Arial;font-weight:700;color:#fff;font-size:16px;">S</span><span style="font-family:Arial;font-weight:700;color:#fff;font-size:16px;">train</span><span style="font-family:Arial;font-weight:700;color:#fff;font-size:16px;">s</span>
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      <span style="font-family:Arial;font-weight:700;font-style:italic;color:#fff;font-size:16px;">E. coli</span><span style="font-family:Arial;color:#fff;font-size:16px;"> strain DH5</span><span style="font-family:Arial;color:#fff;font-size:16px;">α</span><span style="font-family:Arial;color:#fff;font-size:16px;">, was offered by </span><span style="font-family:Arial;color:#fff;font-size:16px;">XMU-China iGEM 2013.</span>
 </p>
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      <span style="font-family:Arial;font-weight:700;font-style:italic;color:#fff;font-size:16px;">E. coli</span><span style="font-family:Arial;color:#fff;font-size:16px;"> strain </span><span style="font-family:Arial;color:#fff;font-size:16px;">CL-1</span><span style="font-family:Arial;color:#fff;font-size:16px;">, </span><span style="font-family:Arial;color:#fff;font-size:16px;">whose gene of</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> cheZ</span><span style="font-family:Arial;color:#fff;font-size:16px;"> had been knocked out, </span><span style="font-family:Arial;color:#fff;font-size:16px;">was offered generously by </span><span style="font-family:Arial;color:#fff;font-size:16px;">iGEMer </span><span style="font-family:Arial;color:#fff;font-size:16px;">JianDong Huang and Lizhong Liu, from Department of Biochemistry, </span><span style="font-family:Arial;color:#fff;font-size:16px;">The University of Hong Kong.</span>
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      <span style="font-family:Arial;font-weight:700;font-size:16px;">Antibiotic</span>
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-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">All the antibiotics above require filtration to </span><span style="font-family:Arial;font-size:16px;">sterilize</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >All the antibiotics above require filtration to </span><span >sterilize</span><span >.</span>
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-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Stock in -20°C</span>
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-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">LB </span><span style="font-family:Arial;font-weight:700;font-size:16px;">Liquid </span><span style="font-family:Arial;font-weight:700;font-size:16px;">Medium</span>
 </p>
@@ Line 799: / Line 799: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">Preparation of </span><span style="font-family:Arial;font-weight:700;font-size:16px;">Competent Cell (</span><span style="font-family:Arial;font-size:16px;">TaKaRa Competent Cell Preparation Kit</span><span style="font-family:Arial;font-size:16px;">)</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">Preparation of </span><span style="font-family:Arial;font-weight:700;font-size:16px;">Competent Cell (</span><span >TaKaRa Competent Cell Preparation Kit</span><span >)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Activate the </span><span style="font-family:Arial;font-style:italic;font-size:16px;">E.</span><span style="font-family:Arial;font-style:italic;font-size:16px;"> </span><span style="font-family:Arial;font-style:italic;font-size:16px;">coli </span><span style="font-family:Arial;font-size:16px;">strain on LB-plate from glycerol stock under the condition of 37°C for 12 hours.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Activate the </span><span style="font-family:Arial;font-style:italic;font-size:16px;">E.</span><span style="font-family:Arial;font-style:italic;font-size:16px;"> </span><span style="font-family:Arial;font-style:italic;font-size:16px;">coli </span><span >strain on LB-plate from glycerol stock under the condition of 37°C for 12 hours.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Pre-culture </span><span style="font-family:Arial;font-size:16px;">a single </span><span style="font-family:Arial;font-size:16px;">colony in 5 mL LB </span><span style="font-family:Arial;font-size:16px;">liquid </span><span style="font-family:Arial;font-size:16px;">medium under the condition of 37°C, 200 rpm for 12 hours.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Pre-culture </span><span >a single </span><span >colony in 5 mL LB </span><span >liquid </span><span >medium under the condition of 37°C, 200 rpm for 12 hours.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 200 μL</span><span style="font-family:Arial;font-size:16px;"> pre-culture </span><span style="font-family:Arial;font-size:16px;">bacteria </span><span style="font-family:Arial;font-size:16px;">into 20 mL LB liquid medium. Culture </span><span style="font-family:Arial;font-size:16px;">under the condition of 37°C, 200 rpm for 2 hours.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 200 μL</span><span > pre-culture </span><span >bacteria </span><span >into 20 mL LB liquid medium. Culture </span><span >under the condition of 37°C, 200 rpm for 2 hours.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Place on ice for 30 min.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Place on ice for 30 min.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Subpackage the 20 mL </span><span style="font-family:Arial;font-size:16px;">culture medium</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">into </span><span style="font-family:Arial;font-size:16px;">1.5 mL </span><span style="font-family:Arial;font-size:16px;">sterile </span><span style="font-family:Arial;font-size:16px;">centrifuge tube</span><span style="font-family:Arial;font-size:16px;">, each centrifuge tube containing 1 mL. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Subpackage the 20 mL </span><span >culture medium</span><span > </span><span >into </span><span >1.5 mL </span><span >sterile </span><span >centrifuge tube</span><span >, each centrifuge tube containing 1 mL. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">C</span><span style="font-family:Arial;font-size:16px;">entrifuge</span><span style="font-family:Arial;font-size:16px;"> at 1500×g for 5 min at 4°C, then discard supernatant.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >C</span><span >entrifuge</span><span > at 1500×g for 5 min at 4°C, then discard supernatant.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">R</span><span style="font-family:Arial;font-size:16px;">e-suspend each </span><span style="font-family:Arial;font-size:16px;">centrifuge tube </span><span style="font-family:Arial;font-size:16px;">gently </span><span style="font-family:Arial;font-size:16px;">with 100</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL Solution A</span><span style="font-family:Arial;font-size:16px;">,</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">which has been </span><span style="font-family:Arial;font-size:16px;">precooled in ice</span><span style="font-family:Arial;font-size:16px;">. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >R</span><span >e-suspend each </span><span >centrifuge tube </span><span >gently </span><span >with 100</span><span > </span><span >μL Solution A</span><span >,</span><span > </span><span >which has been </span><span >precooled in ice</span><span >. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Centrifuge at 1500×g for 5 min </span><span style="font-family:Arial;font-size:16px;">at 4°C, then</span><span style="font-family:Arial;font-size:16px;"> discard supernatant.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Centrifuge at 1500×g for 5 min </span><span >at 4°C, then</span><span > discard supernatant.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Re-suspend each </span><span style="font-family:Arial;font-size:16px;">centrifuge tube</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">gently </span><span style="font-family:Arial;font-size:16px;">with 100 μL Solution B</span><span style="font-family:Arial;font-size:16px;">,</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">which has been precooled in ice. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Re-suspend each </span><span >centrifuge tube</span><span > </span><span >gently </span><span >with 100 μL Solution B</span><span >,</span><span > </span><span >which has been precooled in ice. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Stock in -20°C</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Stock in -20°C</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Transformation</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 1 μL </span><span style="font-family:Arial;font-size:16px;">purified </span><span style="font-family:Arial;font-size:16px;">plasmid or 10 μL ligation system into 100 μL fresh competent cells</span><span style="font-family:Arial;font-size:16px;">, which is</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">contained in 1.5 mL centrifuge tube. Then </span><span style="font-family:Arial;font-size:16px;">mix gently.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 1 μL </span><span >purified </span><span >plasmid or 10 μL ligation system into 100 μL fresh competent cells</span><span >, which is</span><span > </span><span >contained in 1.5 mL centrifuge tube. Then </span><span >mix gently.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Leave on ice for 30 minutes.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Leave on ice for 30 minutes.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">H</span><span style="font-family:Arial;font-size:16px;">eat shock at</span><span style="font-family:Arial;font-size:16px;"> 42°C</span><span style="font-family:Arial;font-size:16px;"> for 90 seconds.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >H</span><span >eat shock at</span><span > 42°C</span><span > for 90 seconds.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Leave on ice for 10 minutes immediately.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Leave on ice for 10 minutes immediately.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add </span><span style="font-family:Arial;font-size:16px;">400 μL fresh LB medium into the centrifuge tube</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add </span><span >400 μL fresh LB medium into the centrifuge tube</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Incubate for </span><span style="font-family:Arial;font-size:16px;">2 hours</span><span style="font-family:Arial;font-size:16px;"> under the condition of 37°C, 200 rpm</span><span style="font-family:Arial;font-size:16px;">. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Incubate for </span><span >2 hours</span><span > under the condition of 37°C, 200 rpm</span><span >. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">Note: If </span><span style="font-family:Arial;font-size:16px;">the </span><span style="font-family:Arial;font-size:16px;">resistance </span><span style="font-family:Arial;font-size:16px;">of vector</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">is ampicillin, </span><span style="font-family:Arial;font-size:16px;">incubation</span><span style="font-family:Arial;font-size:16px;"> time</span><span style="font-family:Arial;font-size:16px;"> is </span><span style="font-family:Arial;font-size:16px;">shortened to </span><span style="font-family:Arial;font-size:16px;">1 h</span><span style="font-family:Arial;font-size:16px;">ou</span><span style="font-family:Arial;font-size:16px;">r.</span>
+     <span >Note: If </span><span >the </span><span >resistance </span><span >of vector</span><span > </span><span >is ampicillin, </span><span >incubation</span><span > time</span><span > is </span><span >shortened to </span><span >1 h</span><span >ou</span><span >r.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Spread 100 μL culture medium on a</span><span style="font-family:Arial;font-size:16px;"> LB so</span><span style="font-family:Arial;font-size:16px;">lid medium plate, which contains appropriate antibiotics.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Spread 100 μL culture medium on a</span><span > LB so</span><span >lid medium plate, which contains appropriate antibiotics.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">I</span><span style="font-family:Arial;font-size:16px;">ncubate overnight at 37°C</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >I</span><span >ncubate overnight at 37°C</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Confirmation</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Plasmid Extraction</span><span style="font-family:Arial;font-size:16px;"> (</span><span style="font-family:Arial;font-size:16px;">Omega Plasmid Mini Kit</span><span style="font-family:Arial;font-size:16px;">)</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Plasmid Extraction</span><span > (</span><span >Omega Plasmid Mini Kit</span><span >)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 1 mL overnight suspension culture into a 1.5 mL sterile centrifuge tube. Centrifuge at 10000 x g for 1 min, then discard supernatant.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 1 mL overnight suspension culture into a 1.5 mL sterile centrifuge tube. Centrifuge at 10000 x g for 1 min, then discard supernatant.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Repeat the step above several times, until the amount of bacteria is appropriate.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Repeat the step above several times, until the amount of bacteria is appropriate.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Re</span><span style="font-family:Arial;font-size:16px;">-</span><span style="font-family:Arial;font-size:16px;">suspend </span><span style="font-family:Arial;font-size:16px;">cells gently with</span><span style="font-family:Arial;font-size:16px;"> 250 μL Solution I/RNase A.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Re</span><span >-</span><span >suspend </span><span >cells gently with</span><span > 250 μL Solution I/RNase A.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 250 μL</span><span style="font-family:Arial;font-size:16px;"> Solution II</span><span style="font-family:Arial;font-size:16px;"> into each centrifuge tube</span><span style="font-family:Arial;font-size:16px;">. Mix gently by inverting 4-6 times to obtain </span><span style="font-family:Arial;font-size:16px;">cleared</span><span style="font-family:Arial;font-size:16px;"> lysate. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 250 μL</span><span > Solution II</span><span > into each centrifuge tube</span><span >. Mix gently by inverting 4-6 times to obtain </span><span >cleared</span><span > lysate. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 350 μL</span><span style="font-family:Arial;font-size:16px;"> Solution III </span><span style="font-family:Arial;font-size:16px;">into each centrifuge tube. M</span><span style="font-family:Arial;font-size:16px;">ix well to form </span><span style="font-family:Arial;font-size:16px;">protein </span><span style="font-family:Arial;font-size:16px;">precipitate.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 350 μL</span><span > Solution III </span><span >into each centrifuge tube. M</span><span >ix well to form </span><span >protein </span><span >precipitate.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Centrifuge at </span><span style="font-family:Arial;font-size:16px;">≥</span><span style="font-family:Arial;font-size:16px;">13</span><span style="font-family:Arial;font-size:16px;">000 x g for 10 min.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Centrifuge at </span><span >≥</span><span >13</span><span >000 x g for 10 min.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Transfer cleared lysate into a </span><span style="font-family:Arial;font-size:16px;">HiBind Mini Column</span><span style="font-family:Arial;font-size:16px;"> placed in a 2 mL collection tube. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Transfer cleared lysate into a </span><span >HiBind Mini Column</span><span > placed in a 2 mL collection tube. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Centrifuge at 10000 x g for 1 min</span><span style="font-family:Arial;font-size:16px;">. Discard liquid.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Centrifuge at 10000 x g for 1 min</span><span >. Discard liquid.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Wash </span><span style="font-family:Arial;font-size:16px;">the </span><span style="font-family:Arial;font-size:16px;">HiBind Mini Column</span><span style="font-family:Arial;font-size:16px;"> with 500 μL </span><span style="font-family:Arial;font-size:16px;">HB </span><span style="font-family:Arial;font-size:16px;">Buffer</span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">Centrifuge at 10000 x g for 1 min. Discard liquid.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Wash </span><span >the </span><span >HiBind Mini Column</span><span > with 500 μL </span><span >HB </span><span >Buffer</span><span >.</span><span > </span><span >Centrifuge at 10000 x g for 1 min. Discard liquid.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Using the same collection tube, wash </span><span style="font-family:Arial;font-size:16px;">the HiBind Mini Column</span><span style="font-family:Arial;font-size:16px;"> with 700</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">Μl </span><span style="font-family:Arial;font-size:16px;">DNA Wash Buffer</span><span style="font-family:Arial;font-size:16px;">, which is</span><span style="font-family:Arial;font-size:16px;"> diluted with ethanol.</span><span style="font-family:Arial;font-size:16px;"> Centrifuge at 10000 x g for 1 min. Discard liquid.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Using the same collection tube, wash </span><span >the HiBind Mini Column</span><span > with 700</span><span > </span><span >Μl </span><span >DNA Wash Buffer</span><span >, which is</span><span > diluted with ethanol.</span><span > Centrifuge at 10000 x g for 1 min. Discard liquid.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Wash </span><span style="font-family:Arial;font-size:16px;">the </span><span style="font-family:Arial;font-size:16px;">HiBind Mini Column a second t</span><span style="font-family:Arial;font-size:16px;">ime with an additional 700 μL </span><span style="font-family:Arial;font-size:16px;">DNA Wash Buffer.</span><span style="font-family:Arial;font-size:16px;"> Centrifuge at 10000 x g for 1 min. Discard liquid.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Wash </span><span >the </span><span >HiBind Mini Column a second t</span><span >ime with an additional 700 μL </span><span >DNA Wash Buffer.</span><span > Centrifuge at 10000 x g for 1 min. Discard liquid.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Centrifuge </span><span style="font-family:Arial;font-size:16px;">the </span><span style="font-family:Arial;font-size:16px;">empty HiBind Mini Column at</span><span style="font-family:Arial;font-size:16px;"> ≥ 13000 x g for 2 min</span><span style="font-family:Arial;font-size:16px;"> to </span><span style="font-family:Arial;font-size:16px;">spin-dry</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Centrifuge </span><span >the </span><span >empty HiBind Mini Column at</span><span > ≥ 13000 x g for 2 min</span><span > to </span><span >spin-dry</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Place the HiBind Mini Column into a sterile</span><span style="font-family:Arial;font-size:16px;"> 1.5 mL</span><span style="font-family:Arial;font-size:16px;"> centrifuge tube. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Place the HiBind Mini Column into a sterile</span><span > 1.5 mL</span><span > centrifuge tube. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 30 μL sterile ultrapure water directly into the HiBind Mini Column. Sit at room temperature for 2 minutes. Centrifuge at maximum speed (≥ 13000 x g) for 1 min to elute DNA.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 30 μL sterile ultrapure water directly into the HiBind Mini Column. Sit at room temperature for 2 minutes. Centrifuge at maximum speed (≥ 13000 x g) for 1 min to elute DNA.</span>
 </p>
-<p style="font-family:Times New Roman;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:0em;">
+<p style="font-family:Times New Roman;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial,微软雅黑;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial,微软雅黑;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
      <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial,微软雅黑;font-size:16px;">Digestion for Confirmation</span>
 </p>
-<p style="font-family:Arial,微软雅黑;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial,微软雅黑;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <span style="font-family:Arial,微软雅黑;font-size:16px;">Reaction condition: 37°C for 2 hours.</span>
 </p>
@@ Line 1,088: / Line 1,088: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">DNA gel electrophoresis</span><span style="font-family:Arial;font-size:16px;"> (take 20</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL as example)</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >DNA gel electrophoresis</span><span > (take 20</span><span > </span><span >mL as example)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Dilute </span><span style="font-family:Arial;font-size:16px;">50×TAE to 1×TAE with ddH</span><span style="font-family:Arial;font-size:16px;valign:sub;">2</span><span style="font-family:Arial;font-size:16px;">O.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Dilute </span><span >50×TAE to 1×TAE with ddH</span><span style="font-family:Arial;font-size:16px;valign:sub;">2</span><span >O.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Measure 20 mL</span><span style="font-family:Arial;font-size:16px;"> 1×TAE buffer.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Measure 20 mL</span><span > 1×TAE buffer.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Pour</span><span style="font-family:Arial;font-size:16px;"> 1×TAE buffer </span><span style="font-family:Arial;font-size:16px;">in</span><span style="font-family:Arial;font-size:16px;">to an e</span><span style="font-family:Arial;font-size:16px;">rlenmeyer flask.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Pour</span><span > 1×TAE buffer </span><span >in</span><span >to an e</span><span >rlenmeyer flask.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Weigh</span><span style="font-family:Arial;font-size:16px;"> 0.2g agarose to make 1% </span><span style="font-family:Arial;font-size:16px;">DNA </span><span style="font-family:Arial;font-size:16px;">gel.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Weigh</span><span > 0.2g agarose to make 1% </span><span >DNA </span><span >gel.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Pour</span><span style="font-family:Arial;font-size:16px;"> agarose </span><span style="font-family:Arial;font-size:16px;">in</span><span style="font-family:Arial;font-size:16px;">to </span><span style="font-family:Arial;font-size:16px;">the e</span><span style="font-family:Arial;font-size:16px;">rlenmeyer flask.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Pour</span><span > agarose </span><span >in</span><span >to </span><span >the e</span><span >rlenmeyer flask.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Make</span><span style="font-family:Arial;font-size:16px;"> agarose </span><span style="font-family:Arial;font-size:16px;">melt </span><span style="font-family:Arial;font-size:16px;">by</span><span style="font-family:Arial;font-size:16px;"> microwave, lasting for</span><span style="font-family:Arial;font-size:16px;"> 105</span><span style="font-family:Arial;font-size:16px;"> seconds until melt</span><span style="font-family:Arial;font-size:16px;"> completely</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Make</span><span > agarose </span><span >melt </span><span >by</span><span > microwave, lasting for</span><span > 105</span><span > seconds until melt</span><span > completely</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">C</span><span style="font-family:Arial;font-size:16px;">ool </span><span style="font-family:Arial;font-size:16px;">for 5 minutes</span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-size:16px;"> </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >C</span><span >ool </span><span >for 5 minutes</span><span >.</span><span > </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Assemble gel pouring apparatus by inserting gate into slots.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Assemble gel pouring apparatus by inserting gate into slots.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">P</span><span style="font-family:Arial;font-size:16px;">our</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;font-size:16px;">agarose gel</span><span style="font-family:Arial;font-size:16px;"> into gel tray.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >P</span><span >our</span><span style="font-family:Arial;"> </span><span >agarose gel</span><span > into gel tray.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Bring a piece of silver paper</span><span style="font-family:Arial;font-size:16px;"> on</span><span style="font-family:Arial;font-size:16px;"> the top of the </span><span style="font-family:Arial;font-size:16px;">gel pouring apparatus</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Bring a piece of silver paper</span><span > on</span><span > the top of the </span><span >gel pouring apparatus</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Wait for 30-</span><span style="font-family:Arial;font-size:16px;">40 minutes to solidify the </span><span style="font-family:Arial;font-size:16px;">DNA agarose g</span><span style="font-family:Arial;font-size:16px;">el.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Wait for 30-</span><span >40 minutes to solidify the </span><span >DNA agarose g</span><span >el.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Ligation</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Digestion for</span><span style="font-family:Arial;font-size:16px;"> Ligation</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Digestion for</span><span > Ligation</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">Reaction condition: 37°C for 2.5 hours.</span>
+     <span >Reaction condition: 37°C for 2.5 hours.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:0em;">
      <br/>
 </p>
@@ Line 1,143: / Line 1,143: @@
          <tr style="height:42px;" class="firstRow">
              <td colspan="4" style="width:293px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Insert(A) + Vector(B)</span>
                  </p>
              </td>
              <td colspan="4" style="width:293px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:32px;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:32px;text-align:center;">
                      <span style="font-family:Arial;">Vector (A) + Insert (B)</span>
                  </p>
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:32px;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:32px;text-align:center;">
                      <br/>
                  </p>
@@ Line 1,158: / Line 1,158: @@
          <tr style="height:42px;">
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Insert(A)</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Vector(B)</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,183: / Line 1,183: @@
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Vector(A)</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,200: / Line 1,200: @@
          <tr style="height:42px;">
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;font-style:italic;">Eco</span><span style="font-family:Arial;">R I</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;font-style:italic;">Eco</span><span style="font-family:Arial;">R I</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4.5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,225: / Line 1,225: @@
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;font-style:italic;">Spe</span><span style="font-family:Arial;"> I</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4.5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,242: / Line 1,242: @@
          <tr style="height:42px;">
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;font-style:italic;">Spe</span><span style="font-family:Arial;"> I</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;font-style:italic;">Xba</span><span style="font-family:Arial;"> I</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4.5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,267: / Line 1,267: @@
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;font-style:italic;">Pst</span><span style="font-family:Arial;"> I</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4.5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,284: / Line 1,284: @@
          <tr style="height:42px;">
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10×H Buffer</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10×M Buffer</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10×M Buffer</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10×H Buffer</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,326: / Line 1,326: @@
          <tr style="height:42px;">
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">ddH</span><span style="font-family:Arial;valign:sub;">2</span><span style="font-family:Arial;">O</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">0</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">ddH</span><span style="font-family:Arial;valign:sub;">2</span><span style="font-family:Arial;">O</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">7</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,351: / Line 1,351: @@
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">0</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">ddH</span><span style="font-family:Arial;valign:sub;">2</span><span style="font-family:Arial;">O</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">7</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,368: / Line 1,368: @@
          <tr style="height:42px;">
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Total Volume</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">40</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Total Volume</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">50</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Total Volume</span>
                  </p>
              </td>
              <td style="width:57px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">40</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
              </td>
              <td style="width:85px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Total Volume</span>
                  </p>
              </td>
              <td style="width:66px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">50</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,420: / Line 1,420: @@
      </tbody>
 </table>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Gel Extraction of DNA</span><span style="font-family:Arial;font-size:16px;"> (</span><span style="font-family:Arial;font-size:16px;">Omega Gel Extraction K</span><span style="font-family:Arial;font-size:16px;">it</span><span style="font-family:Arial;font-size:16px;">)</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Gel Extraction of DNA</span><span > (</span><span >Omega Gel Extraction K</span><span >it</span><span >)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Perform</span><span style="font-family:Arial;font-size:16px;"> DNA gel electrophoresis</span><span style="font-family:Arial;font-size:16px;"> to fractionate DNA fragments.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Perform</span><span > DNA gel electrophoresis</span><span > to fractionate DNA fragments.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">When adequate separation of bands has occurred, exci</span><span style="font-family:Arial;font-size:16px;">se the DNA fragments of interest carefully.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >When adequate separation of bands has occurred, exci</span><span >se the DNA fragments of interest carefully.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Weigh in sterile 1.5 mL centrifuge tube</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Weigh in sterile 1.5 mL centrifuge tube</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;font-size:16px;">corresponding</span><span style="font-family:Arial;font-size:16px;"> volume of Binding Buffer (XP2)</span><span style="font-family:Arial;font-size:16px;"> into the centrifuge tube</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add</span><span style="font-family:Arial;"> </span><span >corresponding</span><span > volume of Binding Buffer (XP2)</span><span > into the centrifuge tube</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Heat the DNA/gel mixture at 55-65°C for 7 min, until the gel melt</span><span style="font-family:Arial;font-size:16px;"> completely</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Heat the DNA/gel mixture at 55-65°C for 7 min, until the gel melt</span><span > completely</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Put</span><span style="font-family:Arial;font-size:16px;"> a HiBind DN</span><span style="font-family:Arial;font-size:16px;">A Mini Column in a 2 mL</span><span style="font-family:Arial;font-size:16px;"> collection tube. Add the DNA/</span><span style="font-family:Arial;font-size:16px;">gel</span><span style="font-family:Arial;font-size:16px;"> solution to the HiBind DNA Mini Column.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Put</span><span > a HiBind DN</span><span >A Mini Column in a 2 mL</span><span > collection tube. Add the DNA/</span><span >gel</span><span > solution to the HiBind DNA Mini Column.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Centrifuge at 10000 x g for 1 min at room temperature.</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;font-size:16px;">Discard the flow-through liquid.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Centrifuge at 10000 x g for 1 min at room temperature.</span><span style="font-family:Arial;"> </span><span >Discard the flow-through liquid.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 300 μL</span><span style="font-family:Arial;font-size:16px;"> Binding Buffer (XP2)</span><span style="font-family:Arial;font-size:16px;"> into the centrifuge tube</span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-size:16px;"> Centrifuge at </span><span style="font-family:Arial;font-size:16px;">10</span><span style="font-family:Arial;font-size:16px;">000 x g</span><span style="font-family:Arial;font-size:16px;"> for 1 minute at room temperature. Then discard the flow-through liquid.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 300 μL</span><span > Binding Buffer (XP2)</span><span > into the centrifuge tube</span><span >.</span><span > Centrifuge at </span><span >10</span><span >000 x g</span><span > for 1 minute at room temperature. Then discard the flow-through liquid.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add 700 μL</span><span style="font-family:Arial;font-size:16px;"> SPW Wash Buffer (diluted with absolute ethanol). </span><span style="font-family:Arial;font-size:16px;">Centrifuge at 10</span><span style="font-family:Arial;font-size:16px;">000 x g for 1 min</span><span style="font-family:Arial;font-size:16px;">ute</span><span style="font-family:Arial;font-size:16px;"> at room temperature. Then discard the flow-through liquid</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add 700 μL</span><span > SPW Wash Buffer (diluted with absolute ethanol). </span><span >Centrifuge at 10</span><span >000 x g for 1 min</span><span >ute</span><span > at room temperature. Then discard the flow-through liquid</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Repeat the previous step.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Repeat the previous step.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Centrifuge the empty HiBind Mini Column at ≥ 13000 x g for 2 min to spin-dry.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Centrifuge the empty HiBind Mini Column at ≥ 13000 x g for 2 min to spin-dry.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Place the HiBind </span><span style="font-family:Arial;font-size:16px;">Mini Column into a </span><span style="font-family:Arial;font-size:16px;">sterile</span><span style="font-family:Arial;font-size:16px;"> 1.5 mL</span><span style="font-family:Arial;font-size:16px;"> centrifuge tube. </span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Place the HiBind </span><span >Mini Column into a </span><span >sterile</span><span > 1.5 mL</span><span > centrifuge tube. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">A</span><span style="font-family:Arial;font-size:16px;">dd 3</span><span style="font-family:Arial;font-size:16px;">0 μL Elution Buffer directly i</span><span style="font-family:Arial;font-size:16px;">nto the </span><span style="font-family:Arial;font-size:16px;">HiBind Mini C</span><span style="font-family:Arial;font-size:16px;">olumn</span><span style="font-family:Arial;font-size:16px;">. Sit </span><span style="font-family:Arial;font-size:16px;">at room temperature for 2 minutes. Centrifuge</span><span style="font-family:Arial;font-size:16px;"> at maximum speed (≥ 13</span><span style="font-family:Arial;font-size:16px;">000 x g)</span><span style="font-family:Arial;font-size:16px;"> for 1 min</span><span style="font-family:Arial;font-size:16px;"> to elute DNA.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >A</span><span >dd 3</span><span >0 μL Elution Buffer directly i</span><span >nto the </span><span >HiBind Mini C</span><span >olumn</span><span >. Sit </span><span >at room temperature for 2 minutes. Centrifuge</span><span > at maximum speed (≥ 13</span><span >000 x g)</span><span > for 1 min</span><span > to elute DNA.</span>
 </p>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Ligation</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Ligation</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Reaction condition: 16°C for 5-12 hours.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Reaction condition: 16°C for 5-12 hours.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Measure</span><span style="font-family:Arial;font-size:16px;"> the concentration of DNA fragments and vector which are going to be ligated.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Measure</span><span > the concentration of DNA fragments and vector which are going to be ligated.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Calculate the volume</span><span style="font-family:Arial;font-size:16px;"> of insert </span><span style="font-family:Arial;font-size:16px;">fragments </span><span style="font-family:Arial;font-size:16px;">and vector </span><span style="font-family:Arial;font-size:16px;">which should be added, based on the </span><span style="font-family:Arial;font-size:16px;">formula: </span><img src="http://convert.wenku.baidu.com/rtcs/math?md5sum=50a7536e3d313010db05dc35e367433d.docx&math=23cce6a9e11a7f0381d4959dad7f64fd"/>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Calculate the volume</span><span > of insert </span><span >fragments </span><span >and vector </span><span >which should be added, based on the </span><span >formula: </span><img src="http://convert.wenku.baidu.com/rtcs/math?md5sum=50a7536e3d313010db05dc35e367433d.docx&math=23cce6a9e11a7f0381d4959dad7f64fd"/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:28px;text-indent:-28px;">
-     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span style="font-family:Arial;font-size:16px;">Add </span><span style="font-family:Arial;font-size:16px;">DNA fragments, vector, ligation buffer</span><span style="font-family:Arial;font-size:16px;"> and </span><span style="font-family:Arial;font-size:16px;">T4 Ligase together in an</span><span style="font-family:Arial;font-size:16px;"> EP tube</span><span style="font-family:Arial;font-size:16px;"> as the table below</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-family:Wingdings;"></span><span style="font-family:Wingdings;">	</span><span >Add </span><span >DNA fragments, vector, ligation buffer</span><span > and </span><span >T4 Ligase together in an</span><span > EP tube</span><span > as the table below</span><span >.</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 1,546: / Line 1,546: @@
      </tbody>
 </table>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-size:16px;">Note</span><span style="font-family:Arial;font-size:16px;">: </span><span style="font-family:Arial;font-size:16px;">Mix</span><span style="font-family:Arial;font-size:16px;">ing</span><span style="font-family:Arial;font-size:16px;"> the </span><span style="font-family:Arial;font-size:16px;">ligation system well by pipetting up and down gently is</span><span style="font-family:Arial;font-size:16px;"> necessary</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span >Note</span><span >: </span><span >Mix</span><span >ing</span><span > the </span><span >ligation system well by pipetting up and down gently is</span><span > necessary</span><span >.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">General PCR </span><span style="font-family:Arial;font-size:16px;">(Polymerase Chain Reaction)</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">General PCR </span><span >(Polymerase Chain Reaction)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">PCR System</span>
+     <span >PCR System</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 1,567: / Line 1,567: @@
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,574: / Line 1,574: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Forward Primer</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,586: / Line 1,586: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Reverse Primer</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,598: / Line 1,598: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">dNTP Mixture</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,610: / Line 1,610: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10×PCR Buffer</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,622: / Line 1,622: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">r</span><span style="font-family:Arial;">Taq (TakaRa)</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,634: / Line 1,634: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">ddH</span><span style="font-family:Arial;valign:sub;">2</span><span style="font-family:Arial;">O</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">37</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,646: / Line 1,646: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Total Volume</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">50</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 1,662: / Line 1,662: @@
      </tbody>
 </table>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">PCR Condition</span>
+     <span >PCR Condition</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 1,672: / Line 1,672: @@
          <tr class="firstRow">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 1,694: / Line 1,694: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">s</span>
                  </p>
              </td>
              <td rowspan="3" style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 loops</span>
                  </p>
@@ Line 1,716: / Line 1,716: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Tm°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">s</span>
                  </p>
@@ Line 1,733: / Line 1,733: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1 min/kb</span>
                  </p>
@@ Line 1,750: / Line 1,750: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 1,772: / Line 1,772: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">6</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">15°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;">min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 1,806: / Line 1,806: @@
      <span style="font-family:Arial;font-weight:700;font-size:21px;">Conic curve formation</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span style="font-family:Arial;font-weight:700;font-size:16px;">M63 </span><span style="font-family:Arial;font-weight:700;font-size:16px;valign:sup;">[1]</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> </span><span style="font-family:Arial;font-weight:700;font-size:16px;">semi-solid medium preparation</span>
 </p>
@@ Line 1,971: / Line 1,971: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-size:16px;">Note</span><span style="font-family:Arial;font-size:16px;">：</span>
+     <span >Note</span><span >：</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add</span><span style="font-family:Arial;font-size:16px;"> 0.25</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">g Agar into a erlenmeyer flask first, then pour into 96</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL H</span><span style="font-family:Arial;font-size:16px;valign:sub;">2</span><span style="font-family:Arial;font-size:16px;">O, shaking up</span><span style="font-family:Arial;font-size:16px;">, continue to add</span><span style="font-family:Arial;font-size:16px;"> glycerol using </span><span style="font-family:Arial;font-size:16px;">pipette</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Add</span><span > 0.25</span><span > </span><span >g Agar into a erlenmeyer flask first, then pour into 96</span><span > </span><span >mL H</span><span style="font-family:Arial;font-size:16px;valign:sub;">2</span><span >O, shaking up</span><span >, continue to add</span><span > glycerol using </span><span >pipette</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add 10</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL 0.1</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">g/mL FeSO</span><span style="font-family:Arial;font-size:16px;valign:sub;">4</span><span style="font-family:Arial;font-size:16px;"> solution into the medium.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Add 10</span><span > </span><span >μL 0.1</span><span > </span><span >g/mL FeSO</span><span style="font-family:Arial;font-size:16px;valign:sub;">4</span><span > solution into the medium.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add 10</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL 66</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mg/mL Asp solution into the medium.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Add 10</span><span > </span><span >μL 66</span><span > </span><span >mg/mL Asp solution into the medium.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Met, Leu, His, Thr could be premixed for convenience, and the amino acid should be added by injector through 0.22</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μ</span><span style="font-family:Arial;font-size:16px;">m filter membrane. Pay attention that this process must be carried out after high temperature sterilization of the M63 semi-solid medium.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >Met, Leu, His, Thr could be premixed for convenience, and the amino acid should be added by injector through 0.22</span><span > </span><span >μ</span><span >m filter membrane. Pay attention that this process must be carried out after high temperature sterilization of the M63 semi-solid medium.</span>
 </p>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;">I</span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;">. </span><span style="font-family:Arial;font-weight:700;font-size:16px;">Mix-doting bacteria</span><span style="font-family:Arial;font-weight:700;font-size:16px;">, inductor and inhibitor on </span><span style="font-family:Arial;font-weight:700;font-size:16px;">M63 </span><span style="font-family:Arial;font-weight:700;font-size:16px;">semi-solid medium</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> plate</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> </span><span style="font-family:Arial;font-weight:700;font-size:16px;valign:sup;">[2]</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Pour 20</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">ml M63 semi</span><span style="font-family:Arial;font-size:16px;">-solid medium on a</span><span style="font-family:Arial;font-size:16px;"> pla</span><span style="font-family:Arial;font-size:16px;">te, leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 90 min </span><span style="font-family:Arial;font-size:16px;">in </span><span style="font-family:Arial;font-size:16px;">laminar flow clean bench.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Pour 20</span><span > </span><span >ml M63 semi</span><span >-solid medium on a</span><span > pla</span><span >te, leave the </span><span >plate </span><span >air-dried for 90 min </span><span >in </span><span >laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Dot</span><span style="font-family:Arial;font-size:16px;"> 15</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μ</span><span style="font-family:Arial;font-size:16px;">L inductor (IPTG) or inhibitor (</span><span style="font-family:Arial;color:#333333;font-size:16px;background:#FFFFFF;">L-Arabinose</span><span style="font-family:Arial;font-size:16px;">) on the plate through</span><span style="font-family:Arial;font-size:16px;"> pipette, take care when </span><span style="font-family:Arial;font-size:16px;">insert the tip into the medium. L</span><span style="font-family:Arial;font-size:16px;">eave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 20 min in </span><span style="font-family:Arial;font-size:16px;">laminar flow clean bench</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Dot</span><span > 15</span><span > </span><span >μ</span><span >L inductor (IPTG) or inhibitor (</span>L-Arabinose<span >) on the plate through</span><span > pipette, take care when </span><span >insert the tip into the medium. L</span><span >eave the </span><span >plate </span><span >air-dried for 20 min in </span><span >laminar flow clean bench</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Dot 3</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μ</span><span style="font-family:Arial;font-size:16px;">L culture medium at the </span><span style="font-family:Arial;font-size:16px;">same </span><span style="font-family:Arial;font-size:16px;">position of</span><span style="font-family:Arial;font-size:16px;"> the inductor (IPTG) or inhibitor (</span><span style="font-family:Arial;color:#333333;font-size:16px;background:#FFFFFF;">L-Arabinose</span><span style="font-family:Arial;font-size:16px;">),</span><span style="font-family:Arial;font-size:16px;"> leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 5 min in </span><span style="font-family:Arial;font-size:16px;">laminar flow clean bench</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Dot 3</span><span > </span><span >μ</span><span >L culture medium at the </span><span >same </span><span >position of</span><span > the inductor (IPTG) or inhibitor (</span>L-Arabinose<span >),</span><span > leave the </span><span >plate </span><span >air-dried for 5 min in </span><span >laminar flow clean bench</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Seal</span><span style="font-family:Arial;font-size:16px;"> the plate</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >Seal</span><span > the plate</span><span >.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;">II</span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;">. </span><span style="font-family:Arial;font-weight:700;font-size:16px;">Lineation</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> of inductor and inhibitor on the M63 semi</span><span style="font-family:Arial;font-weight:700;font-size:16px;">-</span><span style="font-family:Arial;font-weight:700;font-size:16px;">solid medium plate</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Pour 20 mL</span><span style="font-family:Arial;font-size:16px;"> M63 semi</span><span style="font-family:Arial;font-size:16px;">-solid medium on a</span><span style="font-family:Arial;font-size:16px;"> plate,</span><span style="font-family:Arial;font-size:16px;"> leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 90 min in </span><span style="font-family:Arial;font-size:16px;">laminar flow clean bench</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Pour 20 mL</span><span > M63 semi</span><span >-solid medium on a</span><span > plate,</span><span > leave the </span><span >plate </span><span >air-dried for 90 min in </span><span >laminar flow clean bench</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Inhale 15</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μ</span><span style="font-family:Arial;font-size:16px;">L inductor (IPTG) or inhibitor (</span><span style="font-family:Arial;color:#333333;font-size:16px;background:#FFFFFF;">L-Arabinose</span><span style="font-family:Arial;font-size:16px;">), doting along the line pre-pianted carefully, bear in mind don’t destroy the semi</span><span style="font-family:Arial;font-size:16px;">-</span><span style="font-family:Arial;font-size:16px;">solid medium when moving along the line.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Inhale 15</span><span > </span><span >μ</span><span >L inductor (IPTG) or inhibitor (</span>L-Arabinose<span >), doting along the line pre-pianted carefully, bear in mind don’t destroy the semi</span><span >-</span><span >solid medium when moving along the line.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 90 min in </span><span style="font-family:Arial;font-size:16px;">laminar flow clean bench.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Leave the </span><span >plate </span><span >air-dried for 90 min in </span><span >laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Repeat the process mix-doting bacteria, inductor and inhibitor on M63 semi-solid medium plate.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >Repeat the process mix-doting bacteria, inductor and inhibitor on M63 semi-solid medium plate.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
@@ Line 2,031: / Line 2,031: @@
      <span style="font-family:Arial;font-weight:700;font-size:16px;">culture</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add</span><span style="font-family:Arial;font-size:16px;"> 50</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL bacterium solution (pLac-RBS (1.0)-</span><span style="font-family:Arial;font-style:italic;font-size:16px;">CheZ</span><span style="font-family:Arial;font-size:16px;">-TT,</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">pLac-RBS (0.3)-</span><span style="font-family:Arial;font-style:italic;font-size:16px;">CheZ</span><span style="font-family:Arial;font-size:16px;">-TT, </span><span style="font-family:Arial;font-size:16px;">pLac-RBS (0.01)-</span><span style="font-family:Arial;font-style:italic;font-size:16px;">CheZ</span><span style="font-family:Arial;font-size:16px;">-TT,</span><span style="font-family:Arial;font-size:16px;">) into 5</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL</span><span style="font-family:Arial;font-size:16px;"> fresh LB liquid medium</span><span style="font-family:Arial;font-size:16px;">,</span><span style="font-family:Arial;font-size:16px;"> in which</span><span style="font-family:Arial;font-size:16px;"> chloromyce</span><span style="font-family:Arial;font-size:16px;">tin concentration is 50</span><span style="font-family:Arial;font-size:16px;"> μ</span><span style="font-family:Arial;font-size:16px;">g/ml.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Add</span><span > 50</span><span > </span><span >μL bacterium solution (pLac-RBS (1.0)-</span><span style="font-family:Arial;font-style:italic;font-size:16px;">CheZ</span><span >-TT,</span><span > </span><span >pLac-RBS (0.3)-</span><span style="font-family:Arial;font-style:italic;font-size:16px;">CheZ</span><span >-TT, </span><span >pLac-RBS (0.01)-</span><span style="font-family:Arial;font-style:italic;font-size:16px;">CheZ</span><span >-TT,</span><span >) into 5</span><span > </span><span >mL</span><span > fresh LB liquid medium</span><span >,</span><span > in which</span><span > chloromyce</span><span >tin concentration is 50</span><span > μ</span><span >g/ml.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Culture overnight under the condition of 37°C, 200 rpm.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Culture overnight under the condition of 37°C, 200 rpm.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add</span><span style="font-family:Arial;font-size:16px;"> 50</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL bacterium solution from the LB </span><span style="font-family:Arial;font-size:16px;">liq</span><span style="font-family:Arial;font-size:16px;">uid medium above to another 5 mL</span><span style="font-family:Arial;font-size:16px;"> fresh</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">LB liquid medium</span><span style="font-family:Arial;font-size:16px;">,</span><span style="font-family:Arial;font-size:16px;"> in which </span><span style="font-family:Arial;font-size:16px;">chloromycetin concentration is 50</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μ</span><span style="font-family:Arial;font-size:16px;">g/mL</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Add</span><span > 50</span><span > </span><span >μL bacterium solution from the LB </span><span >liq</span><span >uid medium above to another 5 mL</span><span > fresh</span><span > </span><span >LB liquid medium</span><span >,</span><span > in which </span><span >chloromycetin concentration is 50</span><span > </span><span >μ</span><span >g/mL</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Culture under the condition of 37°C, 200 rpm </span><span style="font-family:Arial;font-size:16px;">for 3 hours.</span><span style="font-family:Arial;font-size:16px;"> Stock </span><span style="font-family:Arial;font-size:16px;">it in 4</span><span style="font-family:Arial;font-size:16px;">°C.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >Culture under the condition of 37°C, 200 rpm </span><span >for 3 hours.</span><span > Stock </span><span >it in 4</span><span >°C.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">5.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Prepare M63 semi-solid medium as above.</span>
+     <span style="font-size:14px;">5.</span><span style="font-size:14px;">	</span><span >Prepare M63 semi-solid medium as above.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">6.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">D</span><span style="font-family:Arial;font-size:16px;">raw three dots on a </span><span style="font-family:Arial;font-size:16px;">M63 semi-solid medium plate first</span><span style="font-family:Arial;font-size:16px;">, then</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">inoculate</span><span style="font-family:Arial;font-size:16px;"> the three kind</span><span style="font-family:Arial;font-size:16px;">s</span><span style="font-family:Arial;font-size:16px;"> of bacterium solution </span><span style="font-family:Arial;font-size:16px;">at the three</span><span style="font-family:Arial;font-size:16px;"> dot</span><span style="font-family:Arial;font-size:16px;">s, respectively</span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-size:16px;"> The volume</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">of the </span><span style="font-family:Arial;font-size:16px;">inoculate</span><span style="font-family:Arial;font-size:16px;">d bacterium solution is 3 μL.</span>
+     <span style="font-size:14px;">6.</span><span style="font-size:14px;">	</span><span >D</span><span >raw three dots on a </span><span >M63 semi-solid medium plate first</span><span >, then</span><span > </span><span >inoculate</span><span > the three kind</span><span >s</span><span > of bacterium solution </span><span >at the three</span><span > dot</span><span >s, respectively</span><span >.</span><span > The volume</span><span > </span><span >of the </span><span >inoculate</span><span >d bacterium solution is 3 μL.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">7.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">C</span><span style="font-family:Arial;font-size:16px;">ulture in incubator at 37</span><span style="font-family:Arial;font-size:16px;">°C</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">7.</span><span style="font-size:14px;">	</span><span >C</span><span >ulture in incubator at 37</span><span >°C</span><span >.</span>
 </p>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Measurement </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Use a ruler t</span><span style="font-family:Arial;font-size:16px;">o measure the radius</span><span style="font-family:Arial;font-size:16px;"> of colony</span><span style="font-family:Arial;font-size:16px;"> from the bottom of the semi-solid medium</span><span style="font-family:Arial;font-size:16px;"> plate</span><span style="font-family:Arial;font-size:16px;">. The initial colony radius is recorded as R</span><span style="font-family:Arial;font-size:16px;valign:sub;">1</span><span style="font-family:Arial;font-size:16px;">, and the </span><span style="font-family:Arial;font-size:16px;">radiuses</span><span style="font-family:Arial;font-size:16px;"> measured at 12h, 24h, 30h, 36h, 42h... as R2, R3, R4, R5, R6, R7…</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Use a ruler t</span><span >o measure the radius</span><span > of colony</span><span > from the bottom of the semi-solid medium</span><span > plate</span><span >. The initial colony radius is recorded as R</span><span style="font-family:Arial;font-size:16px;valign:sub;">1</span><span >, and the </span><span >radiuses</span><span > measured at 12h, 24h, 30h, 36h, 42h... as R2, R3, R4, R5, R6, R7…</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">R</span><span style="font-family:Arial;font-size:16px;">ecord time and diameter</span><span style="font-family:Arial;font-size:16px;"> in a</span><span style="font-family:Arial;"> </span><span style="font-family:Arial;font-size:16px;">table.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >R</span><span >ecord time and diameter</span><span > in a</span><span style="font-family:Arial;"> </span><span >table.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Process</span><span style="font-family:Arial;font-size:16px;"> data with excel.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Process</span><span > data with excel.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
@@ Line 2,073: / Line 2,073: @@
      <span style="font-family:Arial;font-weight:700;font-size:21px;">B</span><span style="font-family:Arial;font-weight:700;font-size:21px;">lack hole</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Plasmid Construction</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;color:#fff;font-size:16px;">All DNA parts applied in these experiments came from the MIT Registry of Standard Biological Parts.</span><span style="font-family:Arial;color:#fff;font-size:16px;"> The new BioBrick part, P</span><span style="font-family:Arial;color:#fff;font-size:16px;">ompC</span><span style="font-family:Arial;color:#fff;font-size:16px;">-</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;">cheZ </span><span style="font-family:Arial;color:#fff;font-size:16px;">(BBa_K1412010)</span><span style="font-family:Arial;color:#fff;font-size:16px;"> was formed by ligating the pre-existing parts, </span><span style="font-family:Arial;color:#fff;font-size:16px;">P</span><span style="font-family:Arial;color:#fff;font-size:16px;">ompC</span><span style="styleName:A17;font-family:Utopia Std;color:#fff;font-size:7px;font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">(BBa_R0082) and </span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;">cheZ</span><span style="styleName:A17;font-family:Utopia Std;color:#fff;font-size:7px;font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">(BBa_K629003). The E.Z.N.A.</span><span style="font-family:Arial;color:#fff;font-size:16px;valign:sup;">TM</span><span style="font-family:Arial;color:#fff;font-size:16px;"> Plasmid Mini Kit </span><span style="font-family:Arial;color:#fff;font-size:16px;"></span><span style="font-family:Arial;color:#fff;font-size:16px;"></span><span style="font-family:Arial;color:#fff;font-size:16px;"></span><span style="font-family:Arial;color:#fff;font-size:16px;">I</span><span style="font-family:Arial;color:#fff;font-size:16px;"></span><span style="font-family:Arial;color:#fff;font-size:16px;"> (200) was used according to the manufacturer’s instructions to extract plasmid DNA. Bio</span><span style="font-family:Arial;color:#fff;font-size:16px;">Brick parts were digested with restriction enzymes,</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> Eco</span><span style="font-family:Arial;color:#fff;font-size:16px;">RI, </span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;">Xba</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">I, </span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;">Spe</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">I, and</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> Pst</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">I</span><span style="font-family:Arial;font-style:italic;color:#fff;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">to produce ‘sticky ends’ that were used to com</span><span style="font-family:Arial;color:#fff;font-size:16px;">bine BioBrick parts. Digested DNA was purified using 1% aga</span><span style="font-family:Arial;color:#fff;font-size:16px;">rose gel electrophoresis followed by gel purification with the E.Z.N.A.TM Gel Extraction Kit (200). BioBrick parts with matching ‘sticky ends’ were ligated using T4 ligase produced by Takara Biotechnology (Dalian) Co., Ltd. (Takara Dalian). Plasmid DNA was transformed into DH5α</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;color:#fff;font-size:16px;"> first</span><span style="font-family:Arial;color:#fff;font-size:16px;">. Then we began a new circulation consisting of extracting plasmids, digestion and</span><span style="font-family:Arial;color:#fff;font-size:16px;"> DNA gel electrophoresis</span><span style="font-family:Arial;color:#fff;font-size:16px;"> to</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;color:#fff;font-size:16px;">confirm</span><span style="font-family:Arial;color:#fff;font-size:16px;"> the success of the ligation. Finally, the construct was sequenced for verifica</span><span style="font-family:Arial;color:#fff;font-size:16px;">tion.</span>
+     <span style="font-family:Arial;color:#fff;font-size:16px;"> first</span><span style="font-family:Arial;color:#fff;font-size:16px;">. Then we began a new circulation consisting of extracting plasmids, digestion and</span><span style="font-family:Arial;color:#fff;font-size:16px;"> DNA gel electrophoresis</span><span style="font-family:Arial;color:#fff;font-size:16px;"> to</span><span > </span><span style="font-family:Arial;color:#fff;font-size:16px;">confirm</span><span style="font-family:Arial;color:#fff;font-size:16px;"> the success of the ligation. Finally, the construct was sequenced for verifica</span><span style="font-family:Arial;color:#fff;font-size:16px;">tion.</span>
 </p>
 <p style="styleName:mw-headline;styleName:Default Paragraph Font;font-family:Times New Roman;font-size:16px;">
@@ Line 2,094: / Line 2,094: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;">I</span><span style="font-family:Arial;font-weight:700;font-size:16px;"></span><span style="font-family:Arial;font-weight:700;font-size:16px;">. Gradient Plate Experiments (semi-solid medium, Cm 50μg/ml)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span style="font-family:Arial;font-size:16px;">Medium A</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;valign:sup;">[</span><span style="font-family:Arial;font-size:16px;valign:sup;">3</span><span style="font-family:Arial;font-size:16px;valign:sup;">]</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span style="font-family:Arial;font-size:16px;">(Improved)</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span >Medium A</span><span > </span><span style="font-family:Arial;font-size:16px;valign:sup;">[</span><span style="font-family:Arial;font-size:16px;valign:sup;">3</span><span style="font-family:Arial;font-size:16px;valign:sup;">]</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span >(Improved)</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 2,195: / Line 2,195: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">Sucrose concentration gradient: </span><span style="font-family:Arial;font-size:16px;">0~20%, the concentration gradient was 2%.</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">Sucrose concentration gradient: </span><span >0~20%, the concentration gradient was 2%.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Mid-log-phase c</span><span style="font-family:Arial;font-weight:700;font-size:16px;">ell suspensions </span><span style="font-family:Arial;font-weight:700;font-size:16px;">preparation</span><span style="font-family:Arial;font-weight:700;font-size:16px;">:</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Overnight culture</span><span style="font-family:Arial;font-size:16px;"> of the required strain grow</span><span style="font-family:Arial;font-size:16px;"> at 37 °C in Medium A</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span style="font-family:Arial;font-size:16px;">(Improved) </span><span style="font-family:Arial;font-size:16px;">containing appropriate</span><span style="font-family:Arial;font-size:16px;"> chloramphenicol</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Overnight culture</span><span > of the required strain grow</span><span > at 37 °C in Medium A</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span >(Improved) </span><span >containing appropriate</span><span > chloramphenicol</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Dilute the overnight culture</span><span style="font-family:Arial;font-size:16px;"> a</span><span style="font-family:Arial;font-size:16px;">t</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">the ratio of 1:100 in fresh culture</span><span style="font-family:Arial;font-size:16px;"> (Medium A)</span><span style="font-family:Arial;font-size:16px;">, then </span><span style="font-family:Arial;font-size:16px;">incub</span><span style="font-family:Arial;font-size:16px;">ate the culture</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">under the condition of 37°C, 200 rpm</span><span style="font-family:Arial;font-size:16px;">, until </span><span style="font-family:Arial;font-size:16px;">its</span><span style="font-family:Arial;font-size:16px;"> OD590 reaches</span><span style="font-family:Arial;font-size:16px;"> 0.2</span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-size:16px;"> </span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Dilute the overnight culture</span><span > a</span><span >t</span><span > </span><span >the ratio of 1:100 in fresh culture</span><span > (Medium A)</span><span >, then </span><span >incub</span><span >ate the culture</span><span > </span><span >under the condition of 37°C, 200 rpm</span><span >, until </span><span >its</span><span > OD590 reaches</span><span > 0.2</span><span >.</span><span > </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Stock</span><span style="font-family:Arial;font-size:16px;"> in 4</span><span style="font-family:Arial;font-size:16px;">°C.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Stock</span><span > in 4</span><span >°C.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">The method of </span><span style="font-family:Arial;font-weight:700;font-size:16px;">preparing </span><span style="font-family:Arial;font-weight:700;font-size:16px;">gradient semi-solid medium</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> (0.25% agar)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">P</span><span style="font-family:Arial;font-size:16px;">repare 100% sucrose solution</span><span style="font-family:Arial;font-size:16px;">. </span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >P</span><span >repare 100% sucrose solution</span><span >. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">P</span><span style="font-family:Arial;font-size:16px;">repare the medium with tripled ingredients amount</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >P</span><span >repare the medium with tripled ingredients amount</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Put the 100% sucrose solution, the prepared medium and plenty of deionized water into the autoclave for 121</span><span style="font-family:Arial;font-size:16px;">°C</span><span style="font-family:Arial;font-size:16px;">, 20 minutes.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Put the 100% sucrose solution, the prepared medium and plenty of deionized water into the autoclave for 121</span><span >°C</span><span >, 20 minutes.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">After sterilization, put the 100% sucrose solution, the prepared medium and the deionized water into </span><span style="font-family:Arial;font-size:16px;">a </span><span style="font-family:Arial;font-size:16px;">drying oven for around 10 minutes</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >After sterilization, put the 100% sucrose solution, the prepared medium and the deionized water into </span><span >a </span><span >drying oven for around 10 minutes</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">5.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Take 5</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL</span><span style="font-family:Arial;font-size:16px;"> tripled Medium A</span><span style="font-family:Arial;font-size:16px;"> (Improved) </span><span style="font-family:Arial;font-size:16px;">into a 25</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL</span><span style="font-family:Arial;font-size:16px;"> erlenmeyer flask, then </span><span style="font-family:Arial;font-size:16px;">add</span><span style="font-family:Arial;font-size:16px;"> appropriate volume of</span><span style="font-family:Arial;font-size:16px;"> 100% </span><span style="font-family:Arial;font-size:16px;">sucrose solution into </span><span style="font-family:Arial;font-size:16px;">the </span><span style="font-family:Arial;font-size:16px;">erlenmeyer flask</span><span style="font-family:Arial;font-size:16px;">, while the </span><span style="font-family:Arial;font-size:16px;">shortage </span><span style="font-family:Arial;font-size:16px;">will be complemented by deionized water</span><span style="font-family:Arial;font-size:16px;"> to make the total volume to be 15</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">5.</span><span style="font-size:14px;">	</span><span >Take 5</span><span > </span><span >mL</span><span > tripled Medium A</span><span > (Improved) </span><span >into a 25</span><span > </span><span >mL</span><span > erlenmeyer flask, then </span><span >add</span><span > appropriate volume of</span><span > 100% </span><span >sucrose solution into </span><span >the </span><span >erlenmeyer flask</span><span >, while the </span><span >shortage </span><span >will be complemented by deionized water</span><span > to make the total volume to be 15</span><span > </span><span >mL</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">6.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add </span><span style="font-family:Arial;font-size:16px;">15</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL</span><span style="font-family:Arial;font-size:16px;"> chloramphenicol</span><span style="font-family:Arial;font-size:16px;"> into the erlenmeyer flask.</span>
+     <span style="font-size:14px;">6.</span><span style="font-size:14px;">	</span><span >Add </span><span >15</span><span > </span><span >μL</span><span > chloramphenicol</span><span > into the erlenmeyer flask.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">7.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Pour </span><span style="font-family:Arial;font-size:16px;">the mixture into a</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">Petri dish</span><span style="font-family:Arial;font-size:16px;"> (85 mm dia.)</span><span style="font-family:Arial;font-size:16px;"> as quickly as possible.</span>
+     <span style="font-size:14px;">7.</span><span style="font-size:14px;">	</span><span >Pour </span><span >the mixture into a</span><span > </span><span >Petri dish</span><span > (85 mm dia.)</span><span > as quickly as possible.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">8.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 90 min in laminar flow clean bench.</span>
+     <span style="font-size:14px;">8.</span><span style="font-size:14px;">	</span><span >Leave the </span><span >plate </span><span >air-dried for 90 min in laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">9.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">After solidification of the media, cell suspensions from mid-log-phase cultures (</span><span style="font-family:Arial;font-size:16px;">3</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL</span><span style="font-family:Arial;font-size:16px;">) were </span><span style="font-family:Arial;font-size:16px;">inoculated</span><span style="font-family:Arial;font-size:16px;"> in the center of the plate.</span>
+     <span style="font-size:14px;">9.</span><span style="font-size:14px;">	</span><span >After solidification of the media, cell suspensions from mid-log-phase cultures (</span><span >3</span><span > </span><span >μL</span><span >) were </span><span >inoculated</span><span > in the center of the plate.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">10.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave </span><span style="font-family:Arial;font-size:16px;">the plate dried</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">in air for </span><span style="font-family:Arial;font-size:16px;">15 min</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">in laminar flow clean bench.</span>
+     <span style="font-size:14px;">10.</span><span style="font-size:14px;">	</span><span >Leave </span><span >the plate dried</span><span > </span><span >in air for </span><span >15 min</span><span > </span><span >in laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">11.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave the plate incubated at 37</span><span style="font-family:Arial;font-size:16px;">°C. During the period of time, </span><span style="font-family:Arial;font-size:16px;">measure</span><span style="font-family:Arial;font-size:16px;"> the di</span><span style="font-family:Arial;font-size:16px;">ameters of the colony and take</span><span style="font-family:Arial;font-size:16px;"> them down.</span>
+     <span style="font-size:14px;">11.</span><span style="font-size:14px;">	</span><span >Leave the plate incubated at 37</span><span >°C. During the period of time, </span><span >measure</span><span > the di</span><span >ameters of the colony and take</span><span > them down.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-size:16px;"></span><span style="font-family:Arial;font-size:16px;"></span><span style="font-family:Arial;font-size:16px;"></span><span style="font-family:Arial;font-size:16px;">II</span><span style="font-family:Arial;font-size:16px;"></span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> Plates Crossed Experiments (semi-solid medium, Cm 50μg/ml)</span>
+     <span ></span><span ></span><span ></span><span >II</span><span ></span><span >.</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> Plates Crossed Experiments (semi-solid medium, Cm 50μg/ml)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span style="font-family:Arial;font-size:16px;">half Medium A</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span style="font-family:Arial;font-size:16px;">(Improved)</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span >half Medium A</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span >(Improved)</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 2,356: / Line 2,356: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">The concentration of sucrose: </span><span style="font-family:Arial;font-size:16px;">0%, 10%</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">The concentration of sucrose: </span><span >0%, 10%</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">The method of preparing liquid medium containing different concentrations of sucrose:</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Prepare the medium with corresponding ingredients amount in the table above.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Prepare the medium with corresponding ingredients amount in the table above.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add corresponding amount of solid sucrose into the liquid medium</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Add corresponding amount of solid sucrose into the liquid medium</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Wait for sterilization.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Wait for sterilization.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Mid-log-phase cell suspensions preparation: </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Overnight culture of</span><span style="font-family:Arial;font-size:16px;"> the required strain grow at 37</span><span style="font-family:Arial;font-size:16px;">°C in Medium A</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span style="font-family:Arial;font-size:16px;">(I</span><span style="font-family:Arial;font-size:16px;">mproved) containing appropriate</span><span style="font-family:Arial;font-size:16px;"> chloramphenicol.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Overnight culture of</span><span > the required strain grow at 37</span><span >°C in Medium A</span><span style="font-family:Arial;font-size:16px;valign:sup;"> </span><span >(I</span><span >mproved) containing appropriate</span><span > chloramphenicol.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Dilute the overnight culture at the ratio of 1:100 in fresh culture (half Medium A), then incubate the culture </span><span style="font-family:Arial;font-size:16px;">under the condition of 37°C, 200 rpm</span><span style="font-family:Arial;font-size:16px;">, until its OD590 reaches 0.2. </span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Dilute the overnight culture at the ratio of 1:100 in fresh culture (half Medium A), then incubate the culture </span><span >under the condition of 37°C, 200 rpm</span><span >, until its OD590 reaches 0.2. </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Stock in 4°C.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >Stock in 4°C.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:16px;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">The method of preparing semi-solid medium</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> with a cross</span><span style="font-family:Arial;font-weight:700;font-size:16px;"> (0.25% agar)</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Prepare the medium with corresponding ingredients amount in the table above.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Prepare the medium with corresponding ingredients amount in the table above.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Put the</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">medium</span><span style="font-family:Arial;font-size:16px;"> containing agar, the liquid medium containing sucrose and plenty of deionized water </span><span style="font-family:Arial;font-size:16px;">into the autoclave for 121</span><span style="font-family:Arial;font-size:16px;">°C, 20 minutes.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Put the</span><span > </span><span >medium</span><span > containing agar, the liquid medium containing sucrose and plenty of deionized water </span><span >into the autoclave for 121</span><span >°C, 20 minutes.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">After sterilization, put the medium</span><span style="font-family:Arial;font-size:16px;">s and the deionized water</span><span style="font-family:Arial;font-size:16px;"> into a drying oven for around 10 minutes.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >After sterilization, put the medium</span><span >s and the deionized water</span><span > into a drying oven for around 10 minutes.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Take 15</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL</span><span style="font-family:Arial;font-size:16px;"> of the medium</span><span style="font-family:Arial;font-size:16px;"> containing agar</span><span style="font-family:Arial;font-size:16px;"> into a 25</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">mL</span><span style="font-family:Arial;font-size:16px;"> erlenmeyer flask</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >Take 15</span><span > </span><span >mL</span><span > of the medium</span><span > containing agar</span><span > into a 25</span><span > </span><span >mL</span><span > erlenmeyer flask</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">5.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Add 15</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL </span><span style="font-family:Arial;font-size:16px;">chloramphenicol</span><span style="font-family:Arial;font-size:16px;"> into the erlenmeyer flask.</span>
+     <span style="font-size:14px;">5.</span><span style="font-size:14px;">	</span><span >Add 15</span><span > </span><span >μL </span><span >chloramphenicol</span><span > into the erlenmeyer flask.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">6.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Pour the mixture into a </span><span style="font-family:Arial;font-size:16px;">Petri dish (85 mm dia.)</span><span style="font-family:Arial;font-size:16px;"> as quickly as possible.</span>
+     <span style="font-size:14px;">6.</span><span style="font-size:14px;">	</span><span >Pour the mixture into a </span><span >Petri dish (85 mm dia.)</span><span > as quickly as possible.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">7.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 90 min in laminar flow clean bench.</span>
+     <span style="font-size:14px;">7.</span><span style="font-size:14px;">	</span><span >Leave the </span><span >plate </span><span >air-dried for 90 min in laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">8.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">After solidification of the media, </span><span style="font-family:Arial;font-size:16px;">draw a cross composed of two stra</span><span style="font-family:Arial;font-size:16px;">ight lines on the plate, by the way of</span><span style="font-family:Arial;font-size:16px;"> Intensive RBI (1 μL</span><span style="font-family:Arial;font-size:16px;">/mm). O</span><span style="font-family:Arial;font-size:16px;">ne straight line is</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">dotted with water, the other is</span><span style="font-family:Arial;font-size:16px;"> dotted with liquid medium containing 10% sucrose.</span><span style="font-family:Arial;font-size:16px;"> The length of the lines is</span><span style="font-family:Arial;font-size:16px;"> 6 centimeters.</span>
+     <span style="font-size:14px;">8.</span><span style="font-size:14px;">	</span><span >After solidification of the media, </span><span >draw a cross composed of two stra</span><span >ight lines on the plate, by the way of</span><span > Intensive RBI (1 μL</span><span >/mm). O</span><span >ne straight line is</span><span > </span><span >dotted with water, the other is</span><span > dotted with liquid medium containing 10% sucrose.</span><span > The length of the lines is</span><span > 6 centimeters.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">9.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave th</span><span style="font-family:Arial;font-size:16px;">e plate dried in air for 15 min </span><span style="font-family:Arial;font-size:16px;">in laminar flow clean bench.</span>
+     <span style="font-size:14px;">9.</span><span style="font-size:14px;">	</span><span >Leave th</span><span >e plate dried in air for 15 min </span><span >in laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">10.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">C</span><span style="font-family:Arial;font-size:16px;">ell suspensions from mid-log-phase cultures (</span><span style="font-family:Arial;font-size:16px;">3</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">μL</span><span style="font-family:Arial;font-size:16px;">) is</span><span style="font-family:Arial;font-size:16px;"> inoculated </span><span style="font-family:Arial;font-size:16px;">at </span><span style="font-family:Arial;font-size:16px;">the cross point</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">10.</span><span style="font-size:14px;">	</span><span >C</span><span >ell suspensions from mid-log-phase cultures (</span><span >3</span><span > </span><span >μL</span><span >) is</span><span > inoculated </span><span >at </span><span >the cross point</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">11.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave th</span><span style="font-family:Arial;font-size:16px;">e plate dried in air for 15 min </span><span style="font-family:Arial;font-size:16px;">in laminar flow clean bench.</span>
+     <span style="font-size:14px;">11.</span><span style="font-size:14px;">	</span><span >Leave th</span><span >e plate dried in air for 15 min </span><span >in laminar flow clean bench.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">12.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave the plate incubated at 37</span><span style="font-family:Arial;font-size:16px;">°C</span><span style="font-family:Arial;font-size:16px;">.</span><span style="font-family:Arial;font-size:16px;"> During the period of time, </span><span style="font-family:Arial;font-size:16px;">take pictures of the colony.</span>
+     <span style="font-size:14px;">12.</span><span style="font-size:14px;">	</span><span >Leave the plate incubated at 37</span><span >°C</span><span >.</span><span > During the period of time, </span><span >take pictures of the colony.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:14px;">
@@ Line 2,437: / Line 2,437: @@
      <span style="font-family:Arial;font-weight:700;font-size:21px;">Osicllation timer</span>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span style="font-family:Arial;font-size:16px;">Luria–Bertani (LB) medium</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;valign:sup;">[4]</span>
+     <span style="font-family:Arial;font-weight:700;font-size:16px;">Medium: </span><span >Luria–Bertani (LB) medium</span><span > </span><span style="font-family:Arial;font-size:16px;valign:sup;">[4]</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 2,543: / Line 2,543: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-size:16px;">Note</span><span style="font-family:Arial;font-size:16px;">&nbsp;:</span>
+     <span >Note</span><span >&nbsp;:</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Glycerol is used as the carbon source.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Glycerol is used as the carbon source.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">3-4 mL</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">0.1 M HEPES</span><span style="font-family:Arial;font-size:16px;"> </span><span style="font-family:Arial;font-size:16px;">is added after rich defined medium is utilized, </span><span style="font-family:Arial;font-size:16px;">serving</span><span style="font-family:Arial;font-size:16px;"> for</span><span style="font-family:Arial;font-size:16px;"> minimizing the effects of pH.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >3-4 mL</span><span > </span><span >0.1 M HEPES</span><span > </span><span >is added after rich defined medium is utilized, </span><span >serving</span><span > for</span><span > minimizing the effects of pH.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">NaOH is added until PH of the medium reaches 8.0.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >NaOH is added until PH of the medium reaches 8.0.</span>
 </p>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
      <span style="font-family:Arial;font-weight:700;font-size:16px;">Experiments </span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Pour 10 mL</span><span style="font-family:Arial;font-size:16px;"> of the </span><span style="font-family:Arial;font-size:16px;">medium</span><span style="font-family:Arial;font-size:16px;"> above</span><span style="font-family:Arial;font-size:16px;"> containing 0.25% agar (Difco, Bacto agar)</span><span style="font-family:Arial;font-size:16px;"> into a Petridish</span><span style="font-family:Arial;font-size:16px;"> to prepare </span><span style="font-family:Arial;font-size:16px;">semi-solid medium</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">1.</span><span style="font-size:14px;">	</span><span >Pour 10 mL</span><span > of the </span><span >medium</span><span > above</span><span > containing 0.25% agar (Difco, Bacto agar)</span><span > into a Petridish</span><span > to prepare </span><span >semi-solid medium</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Leave the </span><span style="font-family:Arial;font-size:16px;">plate </span><span style="font-family:Arial;font-size:16px;">air-dried for 90 min in laminar flow clean bench </span><span style="font-family:Arial;font-size:16px;">at room temperature</span><span style="font-family:Arial;font-size:16px;">.</span>
+     <span style="font-size:14px;">2.</span><span style="font-size:14px;">	</span><span >Leave the </span><span >plate </span><span >air-dried for 90 min in laminar flow clean bench </span><span >at room temperature</span><span >.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">S</span><span style="font-family:Arial;font-size:16px;">pot medium culture in</span><span style="font-family:Arial;font-size:16px;"> half thick</span><span style="font-family:Arial;font-size:16px;">ness in the centern of the plate.</span>
+     <span style="font-size:14px;">3.</span><span style="font-size:14px;">	</span><span >S</span><span >pot medium culture in</span><span > half thick</span><span >ness in the centern of the plate.</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:24px;text-indent:-24px;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;margin-left:24px;text-indent:-24px;">
-     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Cultivate at 37</span><span style="font-family:Arial;font-size:16px;">°C.</span>
+     <span style="font-size:14px;">4.</span><span style="font-size:14px;">	</span><span >Cultivate at 37</span><span >°C.</span>
 </p>
 <p style="font-family:Times New Roman;font-size:14px;">
@@ Line 2,579: / Line 2,579: @@
      <span style="font-family:Arial;font-weight:700;font-size:21px;">Aptamer key-lock</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">PCR System</span>
+     <span >PCR System</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 2,591: / Line 2,591: @@
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,598: / Line 2,598: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Forward Primer</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,610: / Line 2,610: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Reverse Primer</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,622: / Line 2,622: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">dNTP Mixture</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,634: / Line 2,634: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10×PCR Buffer</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,646: / Line 2,646: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">rTaq (TakaRa)</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,658: / Line 2,658: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">ddH</span><span style="font-family:Arial;valign:sub;">2</span><span style="font-family:Arial;">O</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">37 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,670: / Line 2,670: @@
          <tr>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">Total Volume</span>
                  </p>
              </td>
              <td style="width:277px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">50 </span><span style="font-family:Arial;">μL</span>
                  </p>
@@ Line 2,686: / Line 2,686: @@
      </tbody>
 </table>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">The </span><span style="font-family:Arial;font-size:16px;">source and formation</span><span style="font-family:Arial;font-size:16px;"> of the idea</span>
+     <span >The </span><span >source and formation</span><span > of the idea</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 2,772: / Line 2,772: @@
      </tbody>
 </table>
-<p style="font-family:Times New Roman;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Times New Roman;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">Sequence</span>
+     <span >Sequence</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;width:567px;border-collapse:collapse;border:none;">
@@ Line 2,956: / Line 2,956: @@
      </tbody>
 </table>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">PCR Condition</span><span style="font-family:Arial;font-size:16px;">s</span>
+     <span >PCR Condition</span><span >s</span>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;">
+<p style="font-family:Arial;font-size:16px;;text-indent:2em;text-align:justify;text-indent:0em;">
-     <span style="font-family:Arial;font-size:16px;">Lock</span>
+     <span >Lock</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 2,969: / Line 2,969: @@
          <tr class="firstRow">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 2,991: / Line 2,991: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
              </td>
              <td rowspan="3" style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 loops</span>
                  </p>
@@ Line 3,013: / Line 3,013: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">55°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
@@ Line 3,030: / Line 3,030: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
@@ Line 3,047: / Line 3,047: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,069: / Line 3,069: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">6</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">15°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,100: / Line 3,100: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-size:16px;">Aptamer K</span><span style="font-family:Arial;font-size:16px;">ey</span>
+     <span >Aptamer K</span><span >ey</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 3,107: / Line 3,107: @@
          <tr class="firstRow">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,129: / Line 3,129: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
              </td>
              <td rowspan="3" style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 loops</span>
                  </p>
@@ Line 3,151: / Line 3,151: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">52</span><span style="font-family:Arial;">°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
@@ Line 3,168: / Line 3,168: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
@@ Line 3,185: / Line 3,185: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,207: / Line 3,207: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">6</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">15°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,238: / Line 3,238: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:16px;">
+<p >
-     <span style="font-family:Arial;font-size:16px;">New RBS</span>
+     <span >New RBS</span>
 </p>
 <table style="styleName:Table Grid;font-size:16px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;border-insideH:1px solid #fff;border-insideV:1px solid #fff;styleName:Normal Table;margin-left:0px;cellpadding:0px 7px 0px 7px;border-collapse:collapse;border:none;">
@@ Line 3,245: / Line 3,245: @@
          <tr class="firstRow">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">1</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:1px solid #fff;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,267: / Line 3,267: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">2</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">94°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
              </td>
              <td rowspan="3" style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 loops</span>
                  </p>
@@ Line 3,289: / Line 3,289: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">3</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">55°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
@@ Line 3,306: / Line 3,306: @@
          <tr>
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">4</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">30 s</span>
                  </p>
@@ Line 3,323: / Line 3,323: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">72°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">10 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,345: / Line 3,345: @@
          <tr style="height:5px;">
              <td style="width:137px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:1px solid #fff;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">6</span>
                  </p>
              </td>
              <td style="width:149px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial,MS Mincho;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial,MS Mincho;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">15°C</span>
                  </p>
              </td>
              <td style="width:140px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <span style="font-family:Arial;">5 min</span>
                  </p>
              </td>
              <td style="width:127px;vertical-align:top;padding:0px 7px 0px 7px;border-top:none;border-left:none;border-bottom:1px solid #fff;border-right:1px solid #fff;">
-                 <p style="font-family:Arial;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;text-indent:0em;text-align:center;">
+                 <p style="font-family:Arial;;text-indent:2em;text-align:justify;text-indent:0em;text-align:center;">
                      <br/>
                  </p>
@@ Line 3,382: / Line 3,382: @@
      <br/>
 </p>
-<p style="font-family:Arial;font-size:21px;">
+<p style="font-size: 18px;">
-     <span style="font-family:Arial;font-weight:700;font-size:21px;">References</span>
+     <b>References</b>
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p>
-     <span style="font-size:14px;">[1]</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Adler&nbsp;J.&nbsp;A&nbsp;method&nbsp;for&nbsp;measuring&nbsp;chemotaxis&nbsp;and&nbsp;use&nbsp;of&nbsp;the&nbsp;method&nbsp;to&nbsp;determine&nbsp;optimum&nbsp;conditions&nbsp;for&nbsp;chemotaxis&nbsp;by&nbsp;Escherichia&nbsp;coli[J].&nbsp;Journal&nbsp;of&nbsp;General&nbsp;Microbiology,&nbsp;1973,&nbsp;74(1):&nbsp;77-91. </span>
+     [1]Adler J. A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli[J]. Journal of General Microbiology, 1973, 74(1): 77-91.
 </p>
-<p style="font-family:Arial,;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;text-align:left;">
+<p>
-     <span style="font-size:14px;">[2]</span><span style="font-size:14px;">	</span><span style="font-family:Arial,;font-size:16px;">S&nbsp;T,&nbsp;JP.&nbsp;G.&nbsp;Guiding&nbsp;bacteria&nbsp;with&nbsp;small&nbsp;molecules&nbsp;and&nbsp;RNA[J].&nbsp;Journal&nbsp;of&nbsp;the&nbsp;American&nbsp;Chemical&nbsp;Society,&nbsp;2007,&nbsp;(21):6807-6811. </span>
+     [2]	S T, JP. G. Guiding bacteria with small molecules and RNA[J]. Journal of the American Chemical Society, 2007, (21):6807-6811.
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p>
-     <span style="font-size:14px;">[3]</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Kawaji, H., T. Mizuno, and S. Mizushima. &quot;Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins O-8 and O-9 of Escherichia coli K-12.&quot; Journal of bacteriology 140.3 (1979): 843-847.</span>
+     [3]	Kawaji, H., T. Mizuno, and S. Mizushima. &quot;Influence of molecular size and osmolarity of sugars and dextrans on the synthesis of outer membrane proteins O-8 and O-9 of Escherichia coli K-12.&quot; Journal of bacteriology 140.3 (1979): 843-847.
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;text-align:left;">
+<p>
-     <span style="font-size:14px;">[4]</span><span style="font-size:14px;">	</span><span style="font-family:Arial;font-size:16px;">Liu&nbsp;C,&nbsp;Fu&nbsp;X,&nbsp;Liu&nbsp;L,&nbsp;et&nbsp;al.&nbsp;Sequential&nbsp;establishment&nbsp;of&nbsp;stripe&nbsp;patterns&nbsp;in&nbsp;an&nbsp;expanding&nbsp;cell&nbsp;population[J].&nbsp;Science,&nbsp;2011,&nbsp;334(6053):&nbsp;238-241.</span>
+     [4] Liu C, Fu X, Liu L, et al. Sequential establishment of stripe patterns in an expanding cell population[J]. Science, 2011, 334(6053): 238-241.
 </p>
-<p style="font-family:Arial;font-size:16px;styleName:List Paragraph;text-indent:2em;styleName:Normal;text-align:justify;margin-left:28px;text-indent:-28px;">
+<p>
-     <span style="font-size:14px;">[5]</span><span style="font-size:14px;">	</span><span style="font-family:Arial;color:#222222;font-size:16px;">Topp S, Gallivan J P. Guiding bacteria with small molecules and RNA[J]. Journal of the American Chemical Society, 2007, 129(21): 6807-6811.</span>
+     [5]	Topp S, Gallivan J P. Guiding bacteria with small molecules and RNA[J]. Journal of the American Chemical Society, 2007, 129(21): 6807-6811.
 </p>
-<p style="font-family: Arial; font-size: 16px; text-align: justify; margin-left: 28px; text-indent: 0em;">
+<p>
      <br/>
 </p>