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Team:BNU-China/home.html
From 2014.igem.org
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/e/e4/Bnu_s2.jpg" alt="2" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/e/e4/Bnu_s2.jpg" alt="2" /> </div> | ||
<div class="showcase-caption"> | <div class="showcase-caption"> | ||
| - | Mr <i>E.coli Prometheus</i> is a philanthropic | + | Mr <i>E.coli Prometheus</i> is a philanthropic bacterium. One day, he saw many poor peanuts living in so tough a condition that they couldn't grow up nor to flower. He was determined to do something and help! </div> |
</div> | </div> | ||
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/6/60/Bnu_s3.jpg" alt="3" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/6/60/Bnu_s3.jpg" alt="3" /> </div> | ||
<div class="showcase-caption"> | <div class="showcase-caption"> | ||
| - | + | He turned to us. He asked us for the power to collect molybdate and send them to poor peanuts. We told him: " Since you were not naturally born with the power, for the sake of other lives, you must die after." But he was as determined as ever.</div> | |
</div> | </div> | ||
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/1/11/Bnu_s4.jpg" alt="4" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/1/11/Bnu_s4.jpg" alt="4" /> </div> | ||
<div class="showcase-caption"> | <div class="showcase-caption"> | ||
| - | <i>E.coli Prometheus</i> collected | + | With his power, <i>E.coli Prometheus</i> collected molybdate ...</div> |
</div> | </div> | ||
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/9/9c/Bnu_s5.jpg" alt="5" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/9/9c/Bnu_s5.jpg" alt="5" /> </div> | ||
<div class="showcase-caption"> | <div class="showcase-caption"> | ||
| - | He sent the precious | + | He sent the precious micro-nutrient to those peanuts everyday. The peanuts became more and more healthier and was about to flower ... |
</div> | </div> | ||
</div> | </div> | ||
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<div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/3/3a/Bnu_s6.jpg" alt="4" /> </div> | <div class="showcase-content"> <img src="https://static.igem.org/mediawiki/2014/3/3a/Bnu_s6.jpg" alt="4" /> </div> | ||
<div class="showcase-caption"> | <div class="showcase-caption"> | ||
| - | Sadly, <i>E.coli Prometheus</i> | + | Sadly, <i>E.coli Prometheus</i>passed away before he saw the flowers of peanut. He suicided as promised. His body changed into nutrition for peanut after his death. </br> He is honorable.</div> |
</div> | </div> | ||
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<!-- End Showcase --> | <!-- End Showcase --> | ||
| - | <div class="intro">THE STORY OF <i>E. | + | <div class="intro">THE STORY OF <i>E.COLI PROMETHEUS</i></div> |
<div class="hr2"></div> | <div class="hr2"></div> | ||
<!-- Divider --> | <!-- Divider --> | ||
<!-- Begin Top Columns --> | <!-- Begin Top Columns --> | ||
| - | <h2> | + | <h2>Our work</h2> |
<div class="two-third"> | <div class="two-third"> | ||
| - | <p> | + | <p>The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots. <p/> |
| + | |||
| + | <p>We transferred our designed plasmids into <i>E.coli</i> "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "<i>Prometheus</i>" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. <i>E.coli Prometheus</i> will deliver Mo directly to the root. This opens a new chapter of biological fertilizer. | ||
</div> <!-- End Top Columns --> | </div> <!-- End Top Columns --> | ||
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<div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/b/b6/Bnu_icon1.png" alt="" class="center" width="22%" /><br /> | <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/b/b6/Bnu_icon1.png" alt="" class="center" width="22%" /><br /> | ||
<h4 class="center">Wiki Design</h4> | <h4 class="center">Wiki Design</h4> | ||
| - | <p class="center"> | + | <p class="center">Art design, pictures, figures and the latest experiment results were put on the wiki, an important platform to show our works.</p> |
</div> | </div> | ||
| - | <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/6/62/Bnu_icon2.png" alt="" class="center" | + | <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/6/62/Bnu_icon2.png" alt="" class="center" style="width:42% !important" /><br /> |
| - | <h4 class="center"> | + | <h4 class="center">ModA & INPN</h4> |
| - | <p class="center"> | + | <p class="center">ModA we use in our project can bind the molybdate, and INPN can display ModA on <i>E.coli</i> surface.</p> |
</div> | </div> | ||
<div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/b/bb/Bnu_icon3.png" alt="" class="center" width="22%" /><br /> | <div class="one-fourth"> <img src="https://static.igem.org/mediawiki/2014/b/bb/Bnu_icon3.png" alt="" class="center" width="22%" /><br /> | ||
| - | <h4 class="center"> | + | <h4 class="center">Chemotaxis</h4> |
| - | <p class="center"> | + | <p class="center">We use McfR plasmid to give <i>E.coli</i> the capacity of Chemotaxis, so that those <i>E.coli</i> carrying molybdate can "swim" to the root of peanut.</p> |
</div> | </div> | ||
<div class="one-fourth last"> <img src="https://static.igem.org/mediawiki/2014/f/fc/Bnu_icon4.png" alt="" class="center" width="22%" /><br /> | <div class="one-fourth last"> <img src="https://static.igem.org/mediawiki/2014/f/fc/Bnu_icon4.png" alt="" class="center" width="22%" /><br /> | ||
<h4 class="center">Modeling</h4> | <h4 class="center">Modeling</h4> | ||
| - | <p class="center"> | + | <p class="center">We have made a model of peanut roots and <i>E.coli</i> in surrounding environment to simulate our idea in soil.</p> |
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
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<li> <a href="https://static.igem.org/mediawiki/2014/6/67/Bnu_lw4.jpg" rel="prettyPhoto" title="We consulted farmers about planting peanut in Gaomi."> <span class="overlay zoom">Human Practice</span><img src="https://static.igem.org/mediawiki/2014/b/b4/Bnu_c4.jpg" alt="" /> </a> </li> | <li> <a href="https://static.igem.org/mediawiki/2014/6/67/Bnu_lw4.jpg" rel="prettyPhoto" title="We consulted farmers about planting peanut in Gaomi."> <span class="overlay zoom">Human Practice</span><img src="https://static.igem.org/mediawiki/2014/b/b4/Bnu_c4.jpg" alt="" /> </a> </li> | ||
| - | <li> <a href="https://static.igem.org/mediawiki/2014/ | + | <li> <a href="https://static.igem.org/mediawiki/2014/b/bf/Bnu_0135.jpg" rel="prettyPhoto" title="This picture shows the chemotaxis of different <i>E.coli</i> in different acids."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/6/68/Bnu_c5.jpg" alt="" /> </a> </li> |
| - | <li> <a href="https://static.igem.org/mediawiki/2014/f/f3/Bnu_c6.jpg" rel="prettyPhoto" title=" | + | |
| + | <li> <a href="https://static.igem.org/mediawiki/2014/f/f8/Bnu_cb6.jpg" rel="prettyPhoto" title="Capillary assay:The capillary tubes were incubated in the bacterial solution."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/f/f3/Bnu_c6.jpg" alt="" /></a></li> | ||
| + | |||
| + | <li> <a href="https://static.igem.org/mediawiki/2014/7/70/Bnu_cb7.jpg" rel="prettyPhoto" title="Agar assay:The bacteria is in the middle filter paper ,the attractant is on the left ,the buffer is on the right.The bacterial chemotaxis towads malate is best."> <span class="overlay zoom">Wet Lab</span><img src="https://static.igem.org/mediawiki/2014/f/fe/Bnu_c7.jpg" alt="" /> </a> </li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="sponsors"> | <div class="sponsors"> | ||
<h2 align="left">Our Sponsors</h2> | <h2 align="left">Our Sponsors</h2> | ||
| - | <a href=" | + | <a href="#fake"><img src="https://static.igem.org/mediawiki/2014/c/c7/Bnu_f1.gif" alt="Beijing Normal University" title="Beijing Normal University" style="margin-right:20px;" width="100px" /></a> |
| - | <a href=" | + | <a href="#fake"><img src="https://static.igem.org/mediawiki/2014/3/3c/Bnu_f2.gif" alt="College of Life Sciences,BNU" title="College of Life Sciences,BNU" style="margin-right:20px;" width="100px" /></a> |
| - | <a href=" | + | <a href="#fake"><img src="https://static.igem.org/mediawiki/2014/a/a7/Bnu_f3.jpg" alt="Beijing Normal University" title="BNU" style="margin-right:20px;" width="200px" /></a> |
| - | <a href=" | + | <a href="#fake"><img src="https://static.igem.org/mediawiki/2014/b/bf/BNU_Xinke.jpg" alt="College of Infomation Science and Technology" title="BNU College of Infomation Science and Technology" style="margin-right:20px;" width="110px" /></a> |
| + | <a href="#fake"><img src="https://static.igem.org/mediawiki/2014/d/d9/Bnu_brain.gif" alt="School of Brain and Cognitive Science & National Key Laboratory of Cognitive Neuroscience and Learning" title="School of Brain and Cognitive Science & National Key Laboratory of Cognitive Neuroscience and Learning" style="margin-right:20px;" width="110px" /></a> | ||
Latest revision as of 03:40, 18 October 2014
Maps
We are iGEMers from BNU-China, we are going to tell you a story about E.coli Prometheus ...
Mr E.coli Prometheus is a philanthropic bacterium. One day, he saw many poor peanuts living in so tough a condition that they couldn't grow up nor to flower. He was determined to do something and help!
He turned to us. He asked us for the power to collect molybdate and send them to poor peanuts. We told him: " Since you were not naturally born with the power, for the sake of other lives, you must die after." But he was as determined as ever.
With his power, E.coli Prometheus collected molybdate ...
He sent the precious micro-nutrient to those peanuts everyday. The peanuts became more and more healthier and was about to flower ...
Sadly, E.coli Prometheuspassed away before he saw the flowers of peanut. He suicided as promised. His body changed into nutrition for peanut after his death. He is honorable.
THE STORY OF E.COLI PROMETHEUS
Our work
The ability of rhizobium to fix nitrogen is essential for legumes growth and development. The stronger the ability, the less chemical fertilizer will be used. Molybdenum is a key element in rhizobial nitrogen fixing enzyme, and is also necessary for the growth of plants themselves. However, Mo being heavy mental, abusing it will pollute the environment. Hence, spot application of Mo is of great importance; it would be even better to concentrate the element only around the roots.
We transferred our designed plasmids into E.coli "Prometheus" to make it express a fusion protein: one half of it anchors on the outter membrane and the other side, namely ModA, absorbs molybdate. With the help of organic acid chemotaxis system, our "Prometheus" will head for the roots. A suicide system depending on time is also designed for bio-safety concerns. E.coli Prometheus will deliver Mo directly to the root. This opens a new chapter of biological fertilizer.
Wiki Design
Art design, pictures, figures and the latest experiment results were put on the wiki, an important platform to show our works.
ModA & INPN
ModA we use in our project can bind the molybdate, and INPN can display ModA on E.coli surface.
Chemotaxis
We use McfR plasmid to give E.coli the capacity of Chemotaxis, so that those E.coli carrying molybdate can "swim" to the root of peanut.
Modeling
We have made a model of peanut roots and E.coli in surrounding environment to simulate our idea in soil.
Latest Works
Our Sponsors
The Story of E.coli Prometheus
BNU-China
