Team:Virtus-Parva Mexico/Safety

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                    <h1>Safety
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                        <p class="lead"><i>The Next Generation in Molecular Machinery</i>  
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                    <li><a href="#sec1">Project Overview</a></li>
 
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                    <li><a href="#sec2">The Idea</a></li>
 
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<h1><font size=7>Safety</h1></font>
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                <h2 id="sec1">Safety</h2>
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Like most iGEM teams, our project consists of working with microorganisms that are part of the risk group 1 and as such only need to work in Level 1 safety conditions. With this biosafety level 1, only the standard safety precautions need apply when handling our microorganisms. By biosafety we understand as taking the necessary precautions so as to minimize the risks to our teammates working in the lab, the general public and the environment. The conditions that we work in, for they are safety level 1, include the most basic precautions in any lab. In these conditions, work can be done on open benches and wearing basic protective equipment such as lab coats, protective eye wear and rubber, or nitrile, gloves.  
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                  <p>Like most iGEM teams, our project consists of working with microorganisms that are part of the risk group 1 and as such only need to work in Level 1 safety conditions. With this biosafety level 1, only the standard safety precautions need apply when handling our microorganisms. These conditions refer to the work being done on open benches and wearing basic protective equipment such as lab coats, protective eyewear and rubber, or nitrile when needed, gloves.  
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Before being allowed to be alone in either of the labs without a post-graduate student present to oversee us, we had to take a standard safety course. This course covered the subjects of proper waste disposal, the importance of labelling everything inside a lab and what actions to take in case of an emergency. </p>  
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Before being allowed to be alone in either of the labs without a post-graduate student present to oversee us, we had to take a standard safety course. This course covered the subjects of proper waste disposal, the importance of labelling everything inside a lab and what actions to take in case of an emergency. The most important part in waste disposal was to correctly maintain separate chemical agents that should not mix. For example, making sure an acid and a base are not thrown into the same container.
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                                <h3>Question 1: Would any of your project idea raise safety issues in terms of research safety, public safety or environmental safety?</h3>
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                                The strain of E. Coli we chose to work with, DSM 1103 is considered within Biosafety level 1 and as such is considered an agent with minimal potential hazard to laboratory personnel and the environment. In any case an outbreak were to happen, our E. Coli strain doesn’t contain genes with toxin or antibiotic resistance. In case of contact with eyes or mouth, E. Coli can cause infections and as such we use wear gloves when dealing with our bacteria and dispose them in red safety bags a company will then sterilize before throwing them away.
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                                <h3>Question 2: Are any parts or devices in our project associated with (or known to cause) pathogenicity, infectivity, toxicity, threats to environmental quality or security concerns?</h3>
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                                The biggest risk comes from E. Coli infections to our team handling bacteria, an issue for which we have taken precautions. There are no other infectivity, toxicity of pathogenicity threats from our project. Security concerns are very low, as there are minimal security risks. Materials used are very common in microbiology labs and cannot be used maliciously or weaponized.
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                                <h3>Question 3: Do any of the new BioBricks parts (or devices) that you made this year raise any safety issues?</h3>
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<h1><font color=#FFFFFF>Our Questions</h1></font>
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                                We do not use any BioBricks parts this year, we have designed a device that only affects bacterial systems.  
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<p align="justify"><font size=4 color="#ffffff">
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<i>Question 1: Would any of your project idea raise safety <br>
 +
issues in terms of research safety, public safety or <br>
 +
environmental safety?</i><br>
 +
<br>
 +
The strain of E. Coli we chose to work with, DSM <br>
 +
1103 is considered within Biosafety level 1 and as<br>
 +
such is considered an agent with minimal potential<br>
 +
hazard to laboratory personnel and the environment.<br>
 +
In any case an outbreak were to happen, our E. Coli<br>
 +
strain doesn’t contain genes with toxin or antibiotic<br>
 +
resistance. In case of contact with eyes or mouth, E.<br>
 +
Coli can cause infections and as such we use wear <br>
 +
gloves when dealing with our bacteria and dispose <br>
 +
them in red safety bags a company will then sterilize<br>
 +
before throwing them away.
 +
<br>
 +
<br>
 +
<i>Question 2: Are any parts or devices in our project <br>
 +
associated with (or known to cause) pathogenicity, <br>
 +
infectivity, toxicity, threats to environmental quality<br>
 +
or security concerns?</i>
 +
<br><br>
 +
The biggest risk comes from E. Coli infections to our <br>
 +
team handling bacteria, an issue for which we have <br>
 +
taken precautions. There are no other infectivity, <br>
 +
toxicity of pathogenicity threats from our project.<br>
 +
Security concerns are very low, as there are minimal<br>
 +
security risks. Materials used are very common in <br>
 +
microbiology labs and cannot be used maliciously or <br>
 +
weaponized.
 +
<br>
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<br>
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<h1><font color=#FFFFFF> Further Questions</h1></font>
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<p> <font size=4><font color=#FFFFFF><i> Question 3: Do any of the new BioBricks parts (or devices) that you made this year raise any safety issues?</i>
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<br>
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We do not use any BioBricks parts this year, we have designed a device that only affects bacterial systems.  
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<br>
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<br>
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Question 4: Is there a local biosafety group, committee, or review board at your institution?
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<br>
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The department of Chemical and Biological Sciences is responsible for regulating safety standards and laboratories. The head of the department and its teachers are aware of our project and as such check on us and make sure we are following guidelines. When in doubt, we refer to the biosafety guidelines the CDC has made public.</font></p>
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                                <h3>Question 4: Is there a local biosafety group, committee, or review board at your institution?</h3>
 
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                              The department of Chemical and Biological Sciences is responsible for regulating safety standards and laboratories. The head of the department and its teachers are aware of our project and as such check on us and make sure we are following guidelines. When in doubt, we refer to the biosafety guidelines the CDC has made public.
 
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        if ("undefined" == typeof jQuery) throw new Error("Bootstrap's JavaScript requires jQuery"); +function (a) { "use strict"; function b() { var a = document.createElement("bootstrap"), b = { WebkitTransition: "webkitTransitionEnd", MozTransition: "transitionend", OTransition: "oTransitionEnd otransitionend", transition: "transitionend" }; for (var c in b) if (void 0 !== a.style[c]) return { end: b[c] }; return !1 } a.fn.emulateTransitionEnd = function (b) { var c = !1, d = this; a(this).one(a.support.transition.end, function () { c = !0 }); var e = function () { c || a(d).trigger(a.support.transition.end) }; return setTimeout(e, b), this }, a(function () { a.support.transition = b() }) }(jQuery), +function (a) { "use strict"; var b = '[data-dismiss="alert"]', c = function (c) { a(c).on("click", b, this.close) }; c.prototype.close = function (b) { function c() { f.trigger("closed.bs.alert").remove() } var d = a(this), e = d.attr("data-target"); e || (e = d.attr("href"), e = e && e.replace(/.*(?=#[^\s]*$)/, "")); var f = a(e); b && b.preventDefault(), f.length || (f = d.hasClass("alert") ? d : d.parent()), f.trigger(b = a.Event("close.bs.alert")), b.isDefaultPrevented() || (f.removeClass("in"), a.support.transition && f.hasClass("fade") ? f.one(a.support.transition.end, c).emulateTransitionEnd(150) : c()) }; var d = a.fn.alert; a.fn.alert = function (b) { return this.each(function () { var d = a(this), e = d.data("bs.alert"); e || d.data("bs.alert", e = new c(this)), "string" == typeof b && e[b].call(d) }) }, a.fn.alert.Constructor = c, a.fn.alert.noConflict = function () { return a.fn.alert = d, this }, a(document).on("click.bs.alert.data-api", b, c.prototype.close) }(jQuery), +function (a) { "use strict"; var b = function (c, d) { this.$element = a(c), this.options = a.extend({}, b.DEFAULTS, d), this.isLoading = !1 }; b.DEFAULTS = { loadingText: "loading..." }, b.prototype.setState = function (b) { var c = "disabled", d = this.$element, e = d.is("input") ? "val" : "html", f = d.data(); b += "Text", f.resetText || d.data("resetText", d[e]()), d[e](f[b] || this.options[b]), setTimeout(a.proxy(function () { "loadingText" == b ? (this.isLoading = !0, d.addClass(c).attr(c, c)) : this.isLoading && (this.isLoading = !1, d.removeClass(c).removeAttr(c)) }, this), 0) }, b.prototype.toggle = function () { var a = !0, b = this.$element.closest('[data-toggle="buttons"]'); if (b.length) { var c = this.$element.find("input"); "radio" == c.prop("type") && (c.prop("checked") && this.$element.hasClass("active") ? a = !1 : b.find(".active").removeClass("active")), a && c.prop("checked", !this.$element.hasClass("active")).trigger("change") } a && this.$element.toggleClass("active") }; var c = a.fn.button; a.fn.button = function (c) { return this.each(function () { var d = a(this), e = d.data("bs.button"), f = "object" == typeof c && c; e || d.data("bs.button", e = new b(this, f)), "toggle" == c ? e.toggle() : c && e.setState(c) }) }, a.fn.button.Constructor = b, a.fn.button.noConflict = function () { return a.fn.button = c, this }, a(document).on("click.bs.button.data-api", "[data-toggle^=button]", function (b) { var c = a(b.target); c.hasClass("btn") || (c = c.closest(".btn")), c.button("toggle"), b.preventDefault() }) }(jQuery), +function (a) { "use strict"; var b = function (b, c) { this.$element = a(b), this.$indicators = this.$element.find(".carousel-indicators"), this.options = c, this.paused = this.sliding = this.interval = this.$active = this.$items = null, "hover" == this.options.pause && this.$element.on("mouseenter", a.proxy(this.pause, this)).on("mouseleave", a.proxy(this.cycle, this)) }; b.DEFAULTS = { interval: 5e3, pause: "hover", wrap: !0 }, b.prototype.cycle = function (b) { return b || (this.paused = !1), this.interval && clearInterval(this.interval), this.options.interval && !this.paused && (this.interval = setInterval(a.proxy(this.next, this), this.options.interval)), this }, b.prototype.getActiveIndex = function () { return this.$active = this.$element.find(".item.active"), this.$items = this.$active.parent().children(), this.$items.index(this.$active) }, b.prototype.to = function (b) { var c = this, d = this.getActiveIndex(); return b > this.$items.length - 1 || 0 > b ? void 0 : this.sliding ? this.$element.one("slid.bs.carousel", function () { c.to(b) }) : d == b ? this.pause().cycle() : this.slide(b > d ? "next" : "prev", a(this.$items[b])) }, b.prototype.pause = function (b) { return b || (this.paused = !0), this.$element.find(".next, .prev").length && a.support.transition && (this.$element.trigger(a.support.transition.end), this.cycle(!0)), this.interval = clearInterval(this.interval), this }, b.prototype.next = function () { return this.sliding ? void 0 : this.slide("next") }, b.prototype.prev = function () { return this.sliding ? void 0 : this.slide("prev") }, b.prototype.slide = function (b, c) { var d = this.$element.find(".item.active"), e = c || d[b](), f = this.interval, g = "next" == b ? "left" : "right", h = "next" == b ? "first" : "last", i = this; if (!e.length) { if (!this.options.wrap) return; e = this.$element.find(".item")[h]() } if (e.hasClass("active")) return this.sliding = !1; var j = a.Event("slide.bs.carousel", { relatedTarget: e[0], direction: g }); return this.$element.trigger(j), j.isDefaultPrevented() ? void 0 : (this.sliding = !0, f && this.pause(), this.$indicators.length && (this.$indicators.find(".active").removeClass("active"), this.$element.one("slid.bs.carousel", function () { var b = a(i.$indicators.children()[i.getActiveIndex()]); b && b.addClass("active") })), a.support.transition && this.$element.hasClass("slide") ? (e.addClass(b), e[0].offsetWidth, d.addClass(g), e.addClass(g), d.one(a.support.transition.end, function () { e.removeClass([b, g].join(" ")).addClass("active"), d.removeClass(["active", g].join(" ")), i.sliding = !1, setTimeout(function () { i.$element.trigger("slid.bs.carousel") }, 0) }).emulateTransitionEnd(1e3 * d.css("transition-duration").slice(0, -1))) : (d.removeClass("active"), e.addClass("active"), this.sliding = !1, this.$element.trigger("slid.bs.carousel")), f && this.cycle(), this) }; var c = a.fn.carousel; a.fn.carousel = function (c) { return this.each(function () { var d = a(this), e = d.data("bs.carousel"), f = a.extend({}, b.DEFAULTS, d.data(), "object" == typeof c && c), g = "string" == typeof c ? c : f.slide; e || d.data("bs.carousel", e = new b(this, f)), "number" == typeof c ? e.to(c) : g ? e[g]() : f.interval && e.pause().cycle() }) }, a.fn.carousel.Constructor = b, a.fn.carousel.noConflict = function () { return a.fn.carousel = c, this }, a(document).on("click.bs.carousel.data-api", "[data-slide], [data-slide-to]", function (b) { var c, d = a(this), e = a(d.attr("data-target") || (c = d.attr("href")) && c.replace(/.*(?=#[^\s]+$)/, "")), f = a.extend({}, e.data(), d.data()), g = d.attr("data-slide-to"); g && (f.interval = !1), e.carousel(f), (g = d.attr("data-slide-to")) && e.data("bs.carousel").to(g), b.preventDefault() }), a(window).on("load", function () { a('[data-ride="carousel"]').each(function () { var b = a(this); b.carousel(b.data()) }) }) }(jQuery), +function (a) { "use strict"; var b = function (c, d) { this.$element = a(c), this.options = a.extend({}, b.DEFAULTS, d), this.transitioning = null, this.options.parent && (this.$parent = a(this.options.parent)), this.options.toggle && this.toggle() }; b.DEFAULTS = { toggle: !0 }, b.prototype.dimension = function () { var a = this.$element.hasClass("width"); return a ? "width" : "height" }, b.prototype.show = function () { if (!this.transitioning && !this.$element.hasClass("in")) { var b = a.Event("show.bs.collapse"); if (this.$element.trigger(b), !b.isDefaultPrevented()) { var c = this.$parent && this.$parent.find("> .panel > .in"); if (c && c.length) { var d = c.data("bs.collapse"); if (d && d.transitioning) return; c.collapse("hide"), d || c.data("bs.collapse", null) } var e = this.dimension(); this.$element.removeClass("collapse").addClass("collapsing")[e](0), this.transitioning = 1; var f = function () { this.$element.removeClass("collapsing").addClass("collapse in")[e]("auto"), this.transitioning = 0, this.$element.trigger("shown.bs.collapse") }; if (!a.support.transition) return f.call(this); var g = a.camelCase(["scroll", e].join("-")); this.$element.one(a.support.transition.end, a.proxy(f, this)).emulateTransitionEnd(350)[e](this.$element[0][g]) } } }, b.prototype.hide = function () { if (!this.transitioning && this.$element.hasClass("in")) { var b = a.Event("hide.bs.collapse"); if (this.$element.trigger(b), !b.isDefaultPrevented()) { var c = this.dimension(); this.$element[c](this.$element[c]())[0].offsetHeight, this.$element.addClass("collapsing").removeClass("collapse").removeClass("in"), this.transitioning = 1; var d = function () { this.transitioning = 0, this.$element.trigger("hidden.bs.collapse").removeClass("collapsing").addClass("collapse") }; return a.support.transition ? void this.$element[c](0).one(a.support.transition.end, a.proxy(d, this)).emulateTransitionEnd(350) : d.call(this) } } }, b.prototype.toggle = function () { this[this.$element.hasClass("in") ? "hide" : "show"]() }; var c = a.fn.collapse; a.fn.collapse = function (c) { return this.each(function () { var d = a(this), e = d.data("bs.collapse"), f = a.extend({}, b.DEFAULTS, d.data(), "object" == typeof c && c); !e && f.toggle && "show" == c && (c = !c), e || d.data("bs.collapse", e = new b(this, f)), "string" == typeof c && e[c]() }) }, a.fn.collapse.Constructor = b, a.fn.collapse.noConflict = function () { return a.fn.collapse = c, this }, a(document).on("click.bs.collapse.data-api", "[data-toggle=collapse]", function (b) { var c, d = a(this), e = d.attr("data-target") || b.preventDefault() || (c = d.attr("href")) && c.replace(/.*(?=#[^\s]+$)/, ""), f = a(e), g = f.data("bs.collapse"), h = g ? "toggle" : d.data(), i = d.attr("data-parent"), j = i && a(i); g && g.transitioning || (j && j.find('[data-toggle=collapse][data-parent="' + i + '"]').not(d).addClass("collapsed"), d[f.hasClass("in") ? "addClass" : "removeClass"]("collapsed")), f.collapse(h) }) }(jQuery), +function (a) { "use strict"; function b(b) { a(d).remove(), a(e).each(function () { var d = c(a(this)), e = { relatedTarget: this }; d.hasClass("open") && (d.trigger(b = a.Event("hide.bs.dropdown", e)), b.isDefaultPrevented() || d.removeClass("open").trigger("hidden.bs.dropdown", e)) }) } function c(b) { var c = b.attr("data-target"); c || (c = b.attr("href"), c = c && /#[A-Za-z]/.test(c) && c.replace(/.*(?=#[^\s]*$)/, "")); var d = c && a(c); return d && d.length ? d : b.parent() } var d = ".dropdown-backdrop", e = "[data-toggle=dropdown]", f = function (b) { a(b).on("click.bs.dropdown", this.toggle) }; f.prototype.toggle = function (d) { var e = a(this); if (!e.is(".disabled, :disabled")) { var f = c(e), g = f.hasClass("open"); if (b(), !g) { "ontouchstart" in document.documentElement && !f.closest(".navbar-nav").length && a('<div class="dropdown-backdrop"/>').insertAfter(a(this)).on("click", b); var h = { relatedTarget: this }; if (f.trigger(d = a.Event("show.bs.dropdown", h)), d.isDefaultPrevented()) return; f.toggleClass("open").trigger("shown.bs.dropdown", h), e.focus() } return !1 } }, f.prototype.keydown = function (b) { if (/(38|40|27)/.test(b.keyCode)) { var d = a(this); if (b.preventDefault(), b.stopPropagation(), !d.is(".disabled, :disabled")) { var f = c(d), g = f.hasClass("open"); if (!g || g && 27 == b.keyCode) return 27 == b.which && f.find(e).focus(), d.click(); var h = " li:not(.divider):visible a", i = f.find("[role=menu]" + h + ", [role=listbox]" + h); if (i.length) { var j = i.index(i.filter(":focus")); 38 == b.keyCode && j > 0 && j--, 40 == b.keyCode && j < i.length - 1 && j++, ~j || (j = 0), i.eq(j).focus() } } } }; var g = a.fn.dropdown; a.fn.dropdown = function (b) { return this.each(function () { var c = a(this), d = c.data("bs.dropdown"); d || c.data("bs.dropdown", d = new f(this)), "string" == typeof b && d[b].call(c) }) }, a.fn.dropdown.Constructor = f, a.fn.dropdown.noConflict = function () { return a.fn.dropdown = g, this }, a(document).on("click.bs.dropdown.data-api", b).on("click.bs.dropdown.data-api", ".dropdown form", function (a) { a.stopPropagation() }).on("click.bs.dropdown.data-api", e, f.prototype.toggle).on("keydown.bs.dropdown.data-api", e + ", [role=menu], [role=listbox]", f.prototype.keydown) }(jQuery), +function (a) { "use strict"; var b = function (b, c) { this.options = c, this.$element = a(b), this.$backdrop = this.isShown = null, this.options.remote && this.$element.find(".modal-content").load(this.options.remote, a.proxy(function () { this.$element.trigger("loaded.bs.modal") }, this)) }; b.DEFAULTS = { backdrop: !0, keyboard: !0, show: !0 }, b.prototype.toggle = function (a) { return this[this.isShown ? "hide" : "show"](a) }, b.prototype.show = function (b) { var c = this, d = a.Event("show.bs.modal", { relatedTarget: b }); this.$element.trigger(d), this.isShown || d.isDefaultPrevented() || (this.isShown = !0, this.escape(), this.$element.on("click.dismiss.bs.modal", '[data-dismiss="modal"]', a.proxy(this.hide, this)), this.backdrop(function () { var d = a.support.transition && c.$element.hasClass("fade"); c.$element.parent().length || c.$element.appendTo(document.body), c.$element.show().scrollTop(0), d && c.$element[0].offsetWidth, c.$element.addClass("in").attr("aria-hidden", !1), c.enforceFocus(); var e = a.Event("shown.bs.modal", { relatedTarget: b }); d ? c.$element.find(".modal-dialog").one(a.support.transition.end, function () { c.$element.focus().trigger(e) }).emulateTransitionEnd(300) : c.$element.focus().trigger(e) })) }, b.prototype.hide = function (b) { b && b.preventDefault(), b = a.Event("hide.bs.modal"), this.$element.trigger(b), this.isShown && !b.isDefaultPrevented() && (this.isShown = !1, this.escape(), a(document).off("focusin.bs.modal"), this.$element.removeClass("in").attr("aria-hidden", !0).off("click.dismiss.bs.modal"), a.support.transition && this.$element.hasClass("fade") ? this.$element.one(a.support.transition.end, a.proxy(this.hideModal, this)).emulateTransitionEnd(300) : this.hideModal()) }, b.prototype.enforceFocus = function () { a(document).off("focusin.bs.modal").on("focusin.bs.modal", a.proxy(function (a) { this.$element[0] === a.target || this.$element.has(a.target).length || this.$element.focus() }, this)) }, b.prototype.escape = function () { this.isShown && this.options.keyboard ? this.$element.on("keyup.dismiss.bs.modal", a.proxy(function (a) { 27 == a.which && this.hide() }, this)) : this.isShown || this.$element.off("keyup.dismiss.bs.modal") }, b.prototype.hideModal = function () { var a = this; this.$element.hide(), this.backdrop(function () { a.removeBackdrop(), a.$element.trigger("hidden.bs.modal") }) }, b.prototype.removeBackdrop = function () { this.$backdrop && this.$backdrop.remove(), this.$backdrop = null }, b.prototype.backdrop = function (b) { var c = this.$element.hasClass("fade") ? "fade" : ""; if (this.isShown && this.options.backdrop) { var d = a.support.transition && c; if (this.$backdrop = a('<div class="modal-backdrop ' + c + '" />').appendTo(document.body), this.$element.on("click.dismiss.bs.modal", a.proxy(function (a) { a.target === a.currentTarget && ("static" == this.options.backdrop ? this.$element[0].focus.call(this.$element[0]) : this.hide.call(this)) }, this)), d && this.$backdrop[0].offsetWidth, this.$backdrop.addClass("in"), !b) return; d ? this.$backdrop.one(a.support.transition.end, b).emulateTransitionEnd(150) : b() } else !this.isShown && this.$backdrop ? (this.$backdrop.removeClass("in"), a.support.transition && this.$element.hasClass("fade") ? this.$backdrop.one(a.support.transition.end, b).emulateTransitionEnd(150) : b()) : b && b() }; var c = a.fn.modal; a.fn.modal = function (c, d) { return this.each(function () { var e = a(this), f = e.data("bs.modal"), g = a.extend({}, b.DEFAULTS, e.data(), "object" == typeof c && c); f || e.data("bs.modal", f = new b(this, g)), "string" == typeof c ? f[c](d) : g.show && f.show(d) }) }, a.fn.modal.Constructor = b, a.fn.modal.noConflict = function () { return a.fn.modal = c, this }, a(document).on("click.bs.modal.data-api", '[data-toggle="modal"]', function (b) { var c = a(this), d = c.attr("href"), e = a(c.attr("data-target") || d && d.replace(/.*(?=#[^\s]+$)/, "")), f = e.data("bs.modal") ? "toggle" : a.extend({ remote: !/#/.test(d) && d }, e.data(), c.data()); c.is("a") && b.preventDefault(), e.modal(f, this).one("hide", function () { c.is(":visible") && c.focus() }) }), a(document).on("show.bs.modal", ".modal", function () { a(document.body).addClass("modal-open") }).on("hidden.bs.modal", ".modal", function () { a(document.body).removeClass("modal-open") }) }(jQuery), +function (a) { "use strict"; var b = function (a, b) { this.type = this.options = this.enabled = this.timeout = this.hoverState = this.$element = null, this.init("tooltip", a, b) }; b.DEFAULTS = { animation: !0, placement: "top", selector: !1, template: '<div class="tooltip"><div class="tooltip-arrow"></div><div class="tooltip-inner"></div></div>', trigger: "hover focus", title: "", delay: 0, html: !1, container: !1 }, b.prototype.init = function (b, c, d) { this.enabled = !0, this.type = b, this.$element = a(c), this.options = this.getOptions(d); for (var e = this.options.trigger.split(" "), f = e.length; f--;) { var g = e[f]; if ("click" == g) this.$element.on("click." + this.type, this.options.selector, a.proxy(this.toggle, this)); else if ("manual" != g) { var h = "hover" == g ? "mouseenter" : "focusin", i = "hover" == g ? "mouseleave" : "focusout"; this.$element.on(h + "." + this.type, this.options.selector, a.proxy(this.enter, this)), this.$element.on(i + "." + this.type, this.options.selector, a.proxy(this.leave, this)) } } this.options.selector ? this._options = a.extend({}, this.options, { trigger: "manual", selector: "" }) : this.fixTitle() }, b.prototype.getDefaults = function () { return b.DEFAULTS }, b.prototype.getOptions = function (b) { return b = a.extend({}, this.getDefaults(), this.$element.data(), b), b.delay && "number" == typeof b.delay && (b.delay = { show: b.delay, hide: b.delay }), b }, b.prototype.getDelegateOptions = function () { var b = {}, c = this.getDefaults(); return this._options && a.each(this._options, function (a, d) { c[a] != d && (b[a] = d) }), b }, b.prototype.enter = function (b) { var c = b instanceof this.constructor ? b : a(b.currentTarget)[this.type](this.getDelegateOptions()).data("bs." + this.type); return clearTimeout(c.timeout), c.hoverState = "in", c.options.delay && c.options.delay.show ? void (c.timeout = setTimeout(function () { "in" == c.hoverState && c.show() }, c.options.delay.show)) : c.show() }, b.prototype.leave = function (b) { var c = b instanceof this.constructor ? b : a(b.currentTarget)[this.type](this.getDelegateOptions()).data("bs." + this.type); return clearTimeout(c.timeout), c.hoverState = "out", c.options.delay && c.options.delay.hide ? void (c.timeout = setTimeout(function () { "out" == c.hoverState && c.hide() }, c.options.delay.hide)) : c.hide() }, b.prototype.show = function () { var b = a.Event("show.bs." + this.type); if (this.hasContent() && this.enabled) { if (this.$element.trigger(b), b.isDefaultPrevented()) return; var c = this, d = this.tip(); this.setContent(), this.options.animation && d.addClass("fade"); var e = "function" == typeof this.options.placement ? this.options.placement.call(this, d[0], this.$element[0]) : this.options.placement, f = /\s?auto?\s?/i, g = f.test(e); g && (e = e.replace(f, "") || "top"), d.detach().css({ top: 0, left: 0, display: "block" }).addClass(e), this.options.container ? d.appendTo(this.options.container) : d.insertAfter(this.$element); var h = this.getPosition(), i = d[0].offsetWidth, j = d[0].offsetHeight; if (g) { var k = this.$element.parent(), l = e, m = document.documentElement.scrollTop || document.body.scrollTop, n = "body" == this.options.container ? window.innerWidth : k.outerWidth(), o = "body" == this.options.container ? window.innerHeight : k.outerHeight(), p = "body" == this.options.container ? 0 : k.offset().left; e = "bottom" == e && h.top + h.height + j - m > o ? 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Latest revision as of 03:31, 18 October 2014

Virtus Parva

Safety


Like most iGEM teams, our project consists of working with microorganisms that are part of the risk group 1 and as such only need to work in Level 1 safety conditions. With this biosafety level 1, only the standard safety precautions need apply when handling our microorganisms. By biosafety we understand as taking the necessary precautions so as to minimize the risks to our teammates working in the lab, the general public and the environment. The conditions that we work in, for they are safety level 1, include the most basic precautions in any lab. In these conditions, work can be done on open benches and wearing basic protective equipment such as lab coats, protective eye wear and rubber, or nitrile, gloves.

Before being allowed to be alone in either of the labs without a post-graduate student present to oversee us, we had to take a standard safety course. This course covered the subjects of proper waste disposal, the importance of labelling everything inside a lab and what actions to take in case of an emergency. The most important part in waste disposal was to correctly maintain separate chemical agents that should not mix. For example, making sure an acid and a base are not thrown into the same container.

Our Questions



Question 1: Would any of your project idea raise safety
issues in terms of research safety, public safety or
environmental safety?


The strain of E. Coli we chose to work with, DSM
1103 is considered within Biosafety level 1 and as
such is considered an agent with minimal potential
hazard to laboratory personnel and the environment.
In any case an outbreak were to happen, our E. Coli
strain doesn’t contain genes with toxin or antibiotic
resistance. In case of contact with eyes or mouth, E.
Coli can cause infections and as such we use wear
gloves when dealing with our bacteria and dispose
them in red safety bags a company will then sterilize
before throwing them away.

Question 2: Are any parts or devices in our project
associated with (or known to cause) pathogenicity,
infectivity, toxicity, threats to environmental quality
or security concerns?


The biggest risk comes from E. Coli infections to our
team handling bacteria, an issue for which we have
taken precautions. There are no other infectivity,
toxicity of pathogenicity threats from our project.
Security concerns are very low, as there are minimal
security risks. Materials used are very common in
microbiology labs and cannot be used maliciously or
weaponized.

Further Questions

Question 3: Do any of the new BioBricks parts (or devices) that you made this year raise any safety issues?
We do not use any BioBricks parts this year, we have designed a device that only affects bacterial systems.

Question 4: Is there a local biosafety group, committee, or review board at your institution?
The department of Chemical and Biological Sciences is responsible for regulating safety standards and laboratories. The head of the department and its teachers are aware of our project and as such check on us and make sure we are following guidelines. When in doubt, we refer to the biosafety guidelines the CDC has made public.