Team:Evry/Notebook/Sensing/Phenol/09-18-2014

From 2014.igem.org

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Ligation of all parts <br>
Ligation of all parts <br>
Transformation on <i>E.coli</i> <br>
Transformation on <i>E.coli</i> <br>
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Purification of palsmids with on 4 colonies <br>
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Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies <br>
Digestion with EcoRI <br>
Digestion with EcoRI <br>
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<img src="https://static.igem.org/mediawiki/2014/1/1e/18.09.jpg"/> <br>
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<img src="https://static.igem.org/mediawiki/2014/0/0d/18annot%C3%A9.jpg"/> <br>
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All colonies contain the construction of all parts.
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DIGEST E GFP32DMPR C1-C2-C3-PARENT C4
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<span class="cd-date">Sept 30</span>
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<span class="cd-date">Sept 18</span>
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Latest revision as of 03:30, 18 October 2014

Picture

Assembling of the parts

Ligation of all parts
Transformation on E.coli
Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies
Digestion with EcoRI


All colonies contain the construction of all parts.

Sept 18