Team:Evry/Notebook/Sensing/Phenol/09-18-2014
From 2014.igem.org
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<u> Assembling of the parts </u> <br> | <u> Assembling of the parts </u> <br> | ||
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- | <img src="https://static.igem.org/mediawiki/2014/ | + | Ligation of all parts <br> |
+ | Transformation on <i>E.coli</i> <br> | ||
+ | Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies <br> | ||
+ | Digestion with EcoRI <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2014/0/0d/18annot%C3%A9.jpg"/> <br> | ||
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- | + | All colonies contain the construction of all parts. | |
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- | <span class="cd-date">Sept | + | <span class="cd-date">Sept 18</span> |
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Latest revision as of 03:30, 18 October 2014
Assembling of the parts
Ligation of all parts
Transformation on E.coli
Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies
Digestion with EcoRI
All colonies contain the construction of all parts.