Team:Evry/Notebook/Sensing/Phenol/09-30-2014

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<u> Mutation of the Pst1 site </u> <br>
<u> Mutation of the Pst1 site </u> <br>
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<img src="https://static.igem.org/mediawiki/2014/0/07/30.09.jpg"/> <br>
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Digestion of plasmid containing DmpR + GFP on three strains of <i>E.coli</i> (DH5a, Top10 and Bl21) by EcoR1 (wells 1), Pst1 (wells 2) and EcoR1+Pst1 (wells 3). <br>
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DIGEST E-P-EP fermentas [DmpR4GFP DH5a] +[DmpR4GFP Top10] +[DmpR4GFP BL21]
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<img src="https://static.igem.org/mediawiki/2014/2/26/30.09annot%C3%A9.jpg"/> <br>
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We have the good construction in DH5a AND tOP 10 STRAINS. <BR>
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Latest revision as of 03:30, 18 October 2014

Picture

Mutation of the Pst1 site

Digestion of plasmid containing DmpR + GFP on three strains of E.coli (DH5a, Top10 and Bl21) by EcoR1 (wells 1), Pst1 (wells 2) and EcoR1+Pst1 (wells 3).

We have the good construction in DH5a AND tOP 10 STRAINS.

Sept 30