Team:ZJU-China/Safety

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    <a name="Safety"></a>
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<th>Species</th>
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<th>Strain</th>
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<th>NIH Risk Group</th>
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<th>Disease risk to human?</th>
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<tr><td > <h3> Welcome! </h3></td>
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<td > <h3> Timeline</h3></td>
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<td>E.Coli(K12)</td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<td>DH5-alpha</td>
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<td>1</td>
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<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<ul>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>  
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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<td>E.Coli(K12)</td>
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<td>BL21(DE3)</td>
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<td>1</td>
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<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
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<td>E.Coli(K12)</td>
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<td>DH10-beta</td>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<td>1</td>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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<td>E.Coli(K12)</td>
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<td>BW25113</td>
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<td>1</td>
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<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
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         <ol>
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             <li><b>Lab Safety</b>
             <li><b>Lab Safety</b>
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             <p>Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required the wear protect equipments including lab-gown, long pants, gloves and so on while dealing with chemical and biological regents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.</p>
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             <p>Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves, etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.</p>
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             <li><b>Research Safety</b>
             <li><b>Research Safety</b>
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             <p>All students involved hava been trained to operate equipments, reagents and strains in a safety way. We are not able to do ecperiment until pass the bio-safety examination.</p>
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             <p>All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.</p>
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            <li><b>Public Safety</b>
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            <p>We try to dispel the misunderstanding about genetic organism as the strong concerning among public.</p>
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             <li><b>Environment Safety</b>
             <li><b>Environment Safety</b>
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             <p>Both DH5-α and BL21(DE3) we used don't contain F plasmid, which guarantee our gene would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.</p>
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             <p> All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.</p>
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            <li><b>Reference</b>
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            <p>  <a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia">http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia</a></p>
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            <p>  <a href="http://www.absa.org/riskgroups/">http://www.absa.org/riskgroups/</a></p>
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        <td><img  src="https://static.igem.org/mediawiki/2014/4/47/ZJU_left_arow.png"> </img></td><td> <a href="https://2014.igem.org/Team:ZJU-China/Log">Previous: Lag Log</a></td>
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<gallery perrow="6" caption="Laboratory Pictures" widths={width}px heights={heights}px showfilename={anything}>
 
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Image:Example2_Lab_1.png|The building our lab is in!
 
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Image:Example2_Lab_2.png|The inside of our lab!
 
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
 
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Image:Example2_Lab_4.png|Working in biosafety cabinets
 
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
 
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
 
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Image:Example2_Lab_7.png|We decorated this part of our lab
 
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Image:Example2_Lab_8.png|Whatever else you want
 
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Latest revision as of 03:27, 18 October 2014


 

Species Strain NIH Risk Group Disease risk to human?
E.Coli(K12) DH5-alpha 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) BL21(DE3) 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) DH10-beta 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) BW25113 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.

 

 

Our Safety Protocol

  1. Lab Safety

    Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves, etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.

  2. Research Safety

    All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.

  3. Environment Safety

    All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.

  4. Reference

    http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia

    http://www.absa.org/riskgroups/

Previous: Lag Log