Team:Michigan

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<h1 >WELCOME TO iGEM 2015! </h1>
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Michigan&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href = "https://2014.igem.org/Team:Michigan">Home</a>
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<a href="https://2014.igem.org/Team:Michigan"style="color:#000000">Home </a> </td>
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<a href = "https://2014.igem.org/Team:Michigan/Results/">Results</a>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Michigan"style="color:#000000"> Official Team Profile </a></td>
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<a href = "https://2014.igem.org/Team:Michigan/Future/">Future Plans</a>
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<a href = "https://2014.igem.org/Team:Michigan/Accomplishments/">Accomplishments</a>
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<a href="https://2014.igem.org/Team:Michigan/Notebook"style="color:#000000"> Notebook</a></td>
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<a href = "https://2014.igem.org/Team:Michigan/Human/">Human Practice</a>
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<a href="https://2014.igem.org/Team:Michigan/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Michigan/Attributions"style="color:#000000"> Attributions </a></td>
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<a href = "https://2014.igem.org/Team:Michigan/Economic/">Economic Analysis</a>
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<p> Please be sure to keep these links, your audience will want to find your: </p>
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<li><a href="https://2014.igem.org/Team:Michigan">Home</a> </li>
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<li><a href="https://2014.igem.org/Team:Michigan/Team">Team</a> </li>
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<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Michigan">Official Team Profile</a> </li>
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<li><a href="https://2014.igem.org/Team:Michigan/Safety">Safety</a> </li>
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<p>There are a few wiki requirements teams must follow:</p>
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<li>All pages, images and files must be hosted on the <a href ="https://2014.igem.org/Special:Upload">  2014.igem.org server</a>. </li>
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<li>All pages must be created under the team’s name space.</li>
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<li>As part of your documentation, keep the links from the menu to the left. </li>
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<li>The <a href="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png"> iGEM logo </a> should be placed on the upper part of every page and should link to <a href="https://2014.igem.org/Main_Page">2014.igem.org</a>.</li>
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<p>Visit the <a href="https://2014.igem.org/Wiki_How-To"> Wiki How To page </a> for a complete list of requirements, tips and other useful information. </p>
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<p>We are currently working on providing teams with some easy to use design templates.
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<br> In the meantime you can also view other team wikis for inspiration! Here are some very good examples</p>
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<li> <a href="https://2013.igem.org/Team:SDU-Denmark/"> 2013 SDU Denmark </a> </li>
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<li> <a href="https://2013.igem.org/Team:SYSU-China">2013 SYSU China</a> </li>
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<li> <a href="https://2013.igem.org/Team:Shenzhen_BGIC_ATCG"> 2013 Shenxhen BGIG ATCG </a></li>
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<li> <a href="https://2013.igem.org/Team:Colombia_Uniandes">2013 Colombia Unianades </a></li>
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<p>For a full wiki list, you can visit <a href="https://igem.org/Team_Wikis?year=2013">iGEM 2013 web sites </a> and <a href="https://igem.org/Team_Wikis?year=2012">iGEM 2012 web sites</a>  lists. </p>
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<a href = "https://2014.igem.org/Team:Michigan/Team/">Team</a>
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<a href = "https://2014.igem.org/Team:Michigan/Team/"> Team Members </a>
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<a href = "https://2014.igem.org/Team:Michigan/Sponsors"> Sponsors </a>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<h1><font size="10"> MSBT</font></h1></p><font size="3"><font color="#191970"> University of Michigan iGEM Team </font></font color></p>
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<p style="position:relative;width:903px;top:-14px;left:39px"> <font color="#888888"> Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in <I>E. coli</I> because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing <i>Salmonella</i>, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities. </font>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="">iGEM 2013 calendar</a> </li>
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<li>Have lots of fun! </li>
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<p style="position:absolute;top:566px;left:38px"><font size="6"> <u>Abstract</u></font></p>
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Latest revision as of 03:27, 18 October 2014

<! -- Michigan Synthetic Biology Team -->

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MSBT

University of Michigan iGEM Team

Antibodies allow us to bind targets in a highly selective manner, giving them a paramount role in biotechnology and medicine. Antibody production remains difficult in E. coli because antibodies require an oxidizing environment to properly fold. We have constructed an expression system capable of secreting functional scFv (single chain fragment variable) antibodies by tagging them to OsmY, a naturally secreted protein. Fusions with OsmY are carried through the periplasm into the media; allowing disulfide bonds to form during translocation. We've demonstrated the utility of this system in the context of sensing Salmonella, the pathogen responsible for 23,000 hospitalizations in the US each year. To demonstrate efficacy of our purified scFv antibodies to bind antigen, we compared them to scFv antibodies purified from an industrial system using ELISA. Our construct has the potential of becoming a cost-effective industry applicable method for purifying scFv antibodies, as well as other proteins, in large quantities.

Abstract

Retrieved from "http://2014.igem.org/Team:Michigan"