Team:HNU China/Safety

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<h1 >WELCOME TO iGEM 2014! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:HNU_China/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:HNU_China"style="color:#000000">Home </a> </td>
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<a href="https://2014.igem.org/Team:HNU_China/Team"style="color:#000000"> Team </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=HNU_China"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:HNU_China/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:HNU_China/Parts"style="color:#000000"> Parts</a></td>
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<a href="https://2014.igem.org/Team:HNU_China/Modeling"style="color:#000000"> Modeling</a></td>
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<a href="https://2014.igem.org/Team:HNU_China/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:HNU_China/Attributions"style="color:#000000"> Attributions </a></td>
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<td align ="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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<tr><td > <h3> Welcome! </h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Edit this page!</h3>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<ul>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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For the biosafety reason, we would attach apoptosis system to ensure the engineering fungus shall not raise biological contamination.<br><br>
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Apoptosis or programmed cell death (PCD) is a highly coordinated cellular suicide program. Caspases, cysteine-dependent aspartate-directed proteases are a family of cysteine proteases that play essential roles in apoptosis. In previous studies in yeast, only the over-expression of caspase 8 led to cell death while the over-expression of caspase 3 was not lethal and only retarded yeast growth. In our design, the apoptosis related protein expresses when exposed to the blue light. Totally dark is harsh to fulfill in the industrial practicing, and urge us put the possibility of expression leakage into consideration, when the toxity of caspase may interfere the iron uptaking. Hence, we choose caspase-3, instead of caspase-8, as the apoptosis trigger[fig.1].<br><br>
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Latest revision as of 03:21, 18 October 2014




Safety
For the biosafety reason, we would attach apoptosis system to ensure the engineering fungus shall not raise biological contamination.

Apoptosis or programmed cell death (PCD) is a highly coordinated cellular suicide program. Caspases, cysteine-dependent aspartate-directed proteases are a family of cysteine proteases that play essential roles in apoptosis. In previous studies in yeast, only the over-expression of caspase 8 led to cell death while the over-expression of caspase 3 was not lethal and only retarded yeast growth. In our design, the apoptosis related protein expresses when exposed to the blue light. Totally dark is harsh to fulfill in the industrial practicing, and urge us put the possibility of expression leakage into consideration, when the toxity of caspase may interfere the iron uptaking. Hence, we choose caspase-3, instead of caspase-8, as the apoptosis trigger[fig.1].


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