Team:Groningen/Template/MODULE/projects/parts/secretion

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System against Biofilm
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Secretion system
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When optimistic bacteria infect start infection a wound they often protect themselves with a biofilm. For an effective fight against these infections the biofilm should be broken down. We have assembled a biobrick of dispersinB fused to an USP45tag (BBa_K1365111).  This part is later assembled in the secretion system agains both <i> Staphylococcus aureus</i> and <i>Pseudonomas aeruginosa </i>.
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Infection by <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i> will be battled by <i>Lactococcus lactis</i> using molecules that prevent biofilm formation, disrupt communication between pathogens and kill the pathogen.
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Antibiofilm formation
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Secrection system against Pseudonomas aeruginosa
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We will fight against P. aeruginosa with an dual action system (BBa_K1365120). We assembled from exciting and novel biobricks a system which is able to produce dispersinB and aiiA (BBa_K1365169). The latter is an quorum quenching enzyme which disrupt communication between P. By doing this we try reduce their activity of both dividing and activating their pathogenic genes (source).  
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When bacteria start infection of a wound, they often protect themselves with a biofilm. For an effective fight against these infections, the biofilm should be broken down. We have assembled a BioBrick of <a href="http://http://parts.igem.org/Part:BBa_K1365111">dispersinB fused to an USP45tag</a>.  This part was later assembled in the secretion system, and can work against <i> S. aureus</i> and <i>P. aeruginosa </i>.
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Battling <i>Pseudomonas aeruginosa</i>
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We will fight against P. aeruginosa with an <a href="http://http://parts.igem.org/Part:BBa_K1365120">dual action system</a>, consisting of the new BioBricks <a href="http://http://parts.igem.org/Part:BBa_K1365111">dispersinB</a> and <a href="http://http://parts.igem.org/Part:BBa_K1365169">aiiA</a>. DispersinB works against biofilm formation as described above. AiiA is an quorum quenching enzyme which disrupt communication between </i>P. aeruginosa</i>. By  disrupting communication we try reduce the activity of both dividing and activation of pathogenic genes by <i>P. aeruginosa</i>.<sup>2</sup> 
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Secrection system against Staphylococcus aureus
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Battling <i>Staphylococcus aureus</i>
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The cell wall of the gram-positive bacteria Staphylococcus aures contains Lipid-2 fatty acids. These are perfect targets for the anti-microbial nisin. We designed and assembled nisin in an a novel biobrick (BBa_K1365100) and in the same brick we included the dispersinB gene. With this, again, dual action system, we want to fight of S. aureus.
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The cell wall of the Gram-positive bacterium <i>S. aureus</i> contains Lipid-2 fatty acids. These are perfect targets for the anti-microbial nisin.<sup>3</sup> We designed and assembled nisin in an a novel <a href="http://http://parts.igem.org/Part:BBa_K1365100">BioBrick that combines the expression of dispersinB and nisA</a>. With this second dual action system, we want to fight of infections by <i>S. aureus</i>.
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<div class="item"><a href="http://parts.igem.org/Part:BBa_K1365111">dispersinB (dspB)</a></div>
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<div class="item"><a href="http://parts.igem.org/Part:BBa_K1365000">nisin</a></div>
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<div class="item"><a href="http://parts.igem.org/Part:BBa_K1365169">aiiA</a></div>
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<li>More about;
 
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<a href="http://parts.igem.org/Part:BBa_K1365999"> dispersinB (dspB)</a></li>
 
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<li>More about;
 
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<a href="http://parts.igem.org/Part:BBa_K1365999"> nisin </a></il>
 
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<li>More about;
 
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<a href="http://parts.igem.org/Part:BBa_K1365999"> quorum quenching molecule(aiiA)</a></il></ul></</div>
 
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Latest revision as of 03:20, 18 October 2014

Secretion system
 
Infection by Staphylococcus aureus and Pseudomonas aeruginosa will be battled by Lactococcus lactis using molecules that prevent biofilm formation, disrupt communication between pathogens and kill the pathogen.
 
Antibiofilm formation
When bacteria start infection of a wound, they often protect themselves with a biofilm. For an effective fight against these infections, the biofilm should be broken down. We have assembled a BioBrick of dispersinB fused to an USP45tag. This part was later assembled in the secretion system, and can work against S. aureus and P. aeruginosa .
 
Battling Pseudomonas aeruginosa
We will fight against P. aeruginosa with an dual action system, consisting of the new BioBricks dispersinB and aiiA. DispersinB works against biofilm formation as described above. AiiA is an quorum quenching enzyme which disrupt communication between P. aeruginosa. By disrupting communication we try reduce the activity of both dividing and activation of pathogenic genes by P. aeruginosa.2
 
Battling Staphylococcus aureus
The cell wall of the Gram-positive bacterium S. aureus contains Lipid-2 fatty acids. These are perfect targets for the anti-microbial nisin.3 We designed and assembled nisin in an a novel BioBrick that combines the expression of dispersinB and nisA. With this second dual action system, we want to fight of infections by S. aureus.
 
More about

dispersinB (dspB)
nisin
aiiA
 
 
 
 
 

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