Team:BIT/part.html

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<div><span class="table"><img src="https://static.igem.org/mediawiki/2014/f/f3/BIT_blue.png" style="width:1000px;"></span>
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  <div id="apDiv1">Part Collection</div>
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  <table width="1024" border="1">
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    <tr>
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      <td width="55" rowspan="21">Amplifier</td>
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      <td width="103">BBa_K1407000</td>
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      <td width="844">RhlR CDS (no LVA tag) and terminator,RhlR/PAI2  inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by  adding PAI2,the part could be inducible and expresses RFP promoter.Before use  it,you'd better add a terminator to the part's tag.And add a promoter before  the RhlR as you want.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407001</td>
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      <td>RhlR/PAI2  inducible promoter with RBS (B0030) and RFP,terminator.By  adding terminator,we want to sure the RFP could be expressed accurately.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407002</td>
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      <td>RBS(B0034)+RhlR(LVA+)+RhlR/PAI2  inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by  adding PAI2, the part could be inducible and expresses RFP promoter.Before use  it,you'd better add a terminator to the part's tag.And add a promoter before  the RhlR as you want.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407003</td>
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      <td>constitutive  of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP.With the LasR  promoter existing,by adding PAI1,the part could be inducible and expresses RFP  promoter.Before use it,you'd better add a terminator to the part's tag.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407004</td>
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      <td>LasR/PAI1  Inducible promoter +RBS(B0034)+GFP and Terminator.With the LasR protein  existing,by adding PAI1,the part could be inducible and expresses GFP protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407005</td>
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      <td>LasR/PAI1  Inducible promoter +RBS(B0030)+RFP and Terminator.With the LasR protein  existing,by adding PAI1,the part could be inducible and expresses RFP protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407006</td>
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      <td>constitutive  production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP.With  the RhlR protein existing,by adding PAI2,the part could be inducible and  expresses RFP protein.Before using it,you'd better add a terminator to the  part's tag.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407007</td>
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      <td>RhlR  CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030)  and RFP,terminator.With the RhlR protein existing,by adding PAI2,the part could  be inducible and expresses RFP protein.Adding a promoter before the RhlR as you  want.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407008</td>
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      <td>constitutive  production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and  GFP,terminator.By adding PAI1,the part could be inducible and expresses GFP  protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407009</td>
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      <td>RhlR  protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and  RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP  protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407010</td>
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      <td>constitutive  production of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and  RFP,Terminator.By adding PAI1,the part could be inducible and expresses RFP  protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407011</td>
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      <td>constitutive  promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030)  and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP  protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407012</td>
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      <td>constitutive  promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS  (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and  expresses RFP protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407013</td>
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      <td>onstitutive  production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and  RFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2 inducible  promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part  could be inducible and expresses RFP protein.</td>
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    </tr>
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    <tr>
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      <td>BBa_K1407014</td>
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      <td><table border="1" cellspacing="0" cellpadding="0" width="100%">
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        <tr>
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          <td width="64%" valign="top"><p>constitutive production of LasR,LasR/PAI1    Inducible promoter with RBS(B0030)and RFP,terminator,constitutivepromoter,RhlR    protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and    RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducible and    expresses RFP protein.</p></td>
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        </tr>
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      </table></td>
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    </tr>
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    <tr>
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      <td>BBa_K1407015</td>
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      <td><table border="1" cellspacing="0" cellpadding="0" width="100%">
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        <tr>
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          <td width="64%" valign="top"><p>constitutive production of LasR,LasR/PAI1    Inducible promoter with RBS(B0034)and    GFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2    inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the    part could be inducible and expresses GFP protein;When PAI2 exsits,the part    could be inducible and expresses RFP protein.</p></td>
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        </tr>
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      </table></td>
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    </tr>
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    <tr>
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      <td>BBa_K1407016</td>
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      <td><table border="1" cellspacing="0" cellpadding="0" width="100%">
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        <tr>
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          <td width="64%" valign="top"><p>constitutive production of LasR,LasR/PAI1    Inducible promoter with RBS(B0034)and    GFP,terminator,constitutivepromoter,RhlR protein generator(LVA-) ,RhlR/PAI2    inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the    part could be inducible and expresses GFP protein;When PAI2 exsits,the part    could be inducible and expresses RFP protein.</p></td>
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        </tr>
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      </table></td>
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    </tr>
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  </table>
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  <div>
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    <table width="1024" border="1">
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      <tr>
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        <td width="55" rowspan="21">Sensor</td>
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        <td width="103">BBa_K1407026</td>
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        <td width="844"><p>The recA promoter can response the  radiation.Radiation will start the sos response .The recA protein is the  important part of the sos response system.</p>
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Sequence  and Features</td>
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      </tr>
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      <tr>
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        <td>BBa_K1407027</td>
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        <td>Toggle  switch system with pRecA-LacI-pLac-LexA</td>
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      </tr>
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      <tr>
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        <td>BBa_K1407031</td>
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        <td>Lactose  or IPTG inducible RFP reporter</td>
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      </tr>
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      <tr>
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        <td>BBa_K1407099</td>
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        <td>The  recA promoter can response the radiation.Radiation will start the sos response  .The recA protein is the important part of the sos response system. From BL21</td>
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      </tr>
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      <tr>
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        <td>Ba_K1407036</td>
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        <td><p>Lux pL controlled LuxR with lux  pRautoinducingLuxI(lva tag)- AHL-GFP</p>
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amplifying  GFP reporter</td>
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      </tr>
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      <tr>
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    </table>
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    <p>&nbsp;</p>
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  </div>
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  <p>&nbsp;</p>
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  <p>&nbsp;</p>
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</div>
<div id="main_content" >
<div id="main_content" >
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    <div id="first" class="table">
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      <p style=" position:absolute; top:35px; left:430px; color:#FFFFFF; font-size:24px; letter-spacing: 2px;">Part Collection</p>
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      <div id="more1" style=" display:none;position:absolute; top:100px; width:420px; height:230px; background:rgba(255,255,255,0.7);"></div>
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<center>
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<h3>Amplification</h3>
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<div id="others" style="position:absolute; top:2182px; align=; left: 38px;"center";">
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<p>constitutive promoter+RhlR protein generator(LVA-)+RhlR/PAI2 Inducible promoter+RBS(B0030)+RFP+Terminator
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<img style="width:1024px" src="https://static.igem.org/mediawiki/2014/4/42/BIT_line1.png">
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The max excitation wavelength for the RFP we used is 584nm,and the max emission wavelength is 607nm.
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<div id="logo">
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    We constructed an amplification plasmid,containg RhlR protein(LVA-) and RhlR/PAI2 Inducible promoter with RFP. Then we transfected the plamid to Trans 5α cells, and used the cells to do follow experiments.
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<img  src="https://static.igem.org/mediawiki/2014/6/6b/BIT_logo.png">
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<img src="https://static.igem.org/mediawiki/2014/2/28/BIT_data1.png"/>
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<img src="https://static.igem.org/mediawiki/2014/5/54/BIT_data2.png"/>
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<p>FIG.1 The cell was cultured in LB culture and shaked at 37℃ for 12 hours. Then we added the PAI1 and incubated the cell for another 6 hours. We set 18 samples in parallel , and calculated their average, the results of the experiment are shown.</p>
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<img src="https://static.igem.org/mediawiki/2014/9/93/BIT_data3.png"/>
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<img src="https://static.igem.org/mediawiki/2014/d/d2/BIT_data4.png"/>
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<p>FIG.2 The cell was cultured in LB culture and shaked at 37℃ for 12 hours. Then we added the PAI1 and incubated the cell for another 6 hours. We set 8 samples in parallel each experiment ,and calculated their average, the results of the experiment are shown.
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From the above data and graph,We can see that with the increase of PAI1’s concentration, fluorescence intensity also gradually increased.And at 200umol, the fluorescence intensity reached peak. Still, how the Fluorescense Intensity/OD changes with the dose of PAI1 over 200umol changes needs further test.
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<img src="https://static.igem.org/mediawiki/2014/e/ed/BIT_data5.png"/>
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<img src="https://static.igem.org/mediawiki/2014/d/db/BIT_data6.png"/>
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<p>FIG.3 The cell was cultured in LB culture and shaked at 37℃ for 9 hours. Then we added the PAI1 and incubated the cell for another 8 hours. We set 18 samples in parallel ,and calculated their average, the results of the experiment are shown.</p>
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<img src="https://static.igem.org/mediawiki/2014/6/60/BIT_data7.png"/>
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<img src="https://static.igem.org/mediawiki/2014/1/12/BIT_data8.png"/>
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<p>FIG.4 The cell was cultured in LB culture and shaked at 37℃ for 12 hours. Then we added the PAI1 to the final concentration 200umol.We set 6 samples in parallel ,and calculated their average, the results of the experiment are shown.</p>
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<img src="https://static.igem.org/mediawiki/2014/8/80/BIT_data9.png"/>
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<img src="https://static.igem.org/mediawiki/2014/e/e8/BIT_data10.png"/>
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<p>FIG.5 The cell was cultured in LB culture and shaked at 37℃ for 9 hours.Then we added the PAI1 to the final concentration 200umol.We set 18 samples in parallel ,and calculated their average,the results of the experiment are shown.
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It’s obvious that with the increase of time, fluorescence intensity also increased gradually.
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These data all above provide evidence that autoinducer molecules PAI1 could enter the cell and induce the expression of RhlR/PAI2 Inducible promoter.<br/>
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constitutive promoter+RhlR protein generator(LVA+)+RhlR/PAI2 Inducible promoter+RBS(B0030)+RFP+Terminator
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    The difference between this amplification plasmid we constructed and the one above is the existing of LVA.
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</p>
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<img src="https://static.igem.org/mediawiki/2014/6/67/BIT_data11.png"/>
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<img src="https://static.igem.org/mediawiki/2014/e/ef/BIT_data12.png"/>
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<p>FIG.6 The cell was cultured in LB culture and shaked at 37℃ for 12 hours. Then we added the PAI1 and incubated the cell for another 6 hours. We set 18 samples in parallel , and calculated their average, the results of the experiment are shown.
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We can see the fluoresence intensity is small at each dose. This is because with the existence of LVA’s ,the half-life period of RhlR protein is too short to combine with PAI1.Hence,the promoter cann’t be induced properly.
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From the data,we can confirm that the autoinducer molecules PAI1 could enter the cell and combine with RhlR protein,and then induce the expression of RhlR/PAI2 Inducible promoter.<br/>
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constitutive production of LasR+LasR/PAI1 Inducible promoter +RBS(B0030)+GFP+Terminator
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The max excitation wavelength for the GFP we used is 501nm,and the max emission wavelength is 511nm.
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    It’s another amplification plasmid we constructed.And we planed to use the two plasmids to respond to different sensors.
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</p>
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<img src="https://static.igem.org/mediawiki/2014/5/56/BIT_data13.png"/>
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<img src="https://static.igem.org/mediawiki/2014/b/b4/BIT_data14.png"/>
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<p>FIG.7 The cell was cultured in LB culture and shaked at 37℃ for 9 hours.Then we added the PAI1 and incubated the cell for another 8 hours.We set 8 samples in parallel for each dose ,and calculated their average,the results of the experiment are shown.<br/>
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2.Time Gradient
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</p>
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<img src="https://static.igem.org/mediawiki/2014/3/31/BIT_data15.png"/>
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<img src="https://static.igem.org/mediawiki/2014/e/ef/BIT_data16.png"/>
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<p>FIG.8 The cell was cultured in LB culture and shaked at 37℃ for 9 hours.Then we added the PAI1 to the final concentration 200umol.We set 6 samples in parallel at each time ,and calculated their average,the results of the experiment are shown.</p>
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<h3>SensorA</h3>
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<img src="https://static.igem.org/mediawiki/2014/4/44/BIT_sensor1.png"/>
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<img src="https://static.igem.org/mediawiki/2014/4/49/BIT_sensor2.png"/>
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<img src="https://static.igem.org/mediawiki/2014/5/53/BIT_sensor3.png"/>
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<p>FIG.1 We constructed sensor plasmid, containing …….Then we transfected the plasmid to…… The cell was cultured in LB culture and shaked at 37℃ for 12 hours. Then we exposed the cell to UV at various doses and incubated the cell for another 2 hours.</p>
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<img src="https://static.igem.org/mediawiki/2014/5/56/BIT_sensor4.png"/>
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<img src="https://static.igem.org/mediawiki/2014/6/6f/BIT_sensor5.png"/>
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<p>FIG.2 We constructed sensor plasmid, containing …….Then we transfected the plasmid to…… The cell was cultured in LB culture and shaked at 37℃ for 12 hours . When the growth state of the cell reached stable phase, we added IPTG and incubated the cell for another 8 hours.</p>
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<img style="width:1024px"src="https://static.igem.org/mediawiki/2014/4/42/BIT_line1.png">
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<div id="logo"><img  src="https://static.igem.org/mediawiki/2014/6/6b/BIT_logo.png"></div>
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     <div id="address">
     <div id="address">
     <p>Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081</p>
     <p>Beijing Institute of Technology | 5 South Zhongguancun Street, Haidian DistrictBeijing, China 100081</p>
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Latest revision as of 03:20, 18 October 2014

<!DOCTYPE html> radiation measurement

Part Collection
Amplifier BBa_K1407000 RhlR CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by adding PAI2,the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.And add a promoter before the RhlR as you want.
BBa_K1407001 RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,terminator.By adding terminator,we want to sure the RFP could be expressed accurately.
BBa_K1407002 RBS(B0034)+RhlR(LVA+)+RhlR/PAI2 inducible promoter with RBS (B0030) and RFP.With the RhlR promoter existing,by adding PAI2, the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.And add a promoter before the RhlR as you want.
BBa_K1407003 constitutive of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP.With the LasR promoter existing,by adding PAI1,the part could be inducible and expresses RFP promoter.Before use it,you'd better add a terminator to the part's tag.
BBa_K1407004 LasR/PAI1 Inducible promoter +RBS(B0034)+GFP and Terminator.With the LasR protein existing,by adding PAI1,the part could be inducible and expresses GFP protein.
BBa_K1407005 LasR/PAI1 Inducible promoter +RBS(B0030)+RFP and Terminator.With the LasR protein existing,by adding PAI1,the part could be inducible and expresses RFP protein.
BBa_K1407006 constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP.With the RhlR protein existing,by adding PAI2,the part could be inducible and expresses RFP protein.Before using it,you'd better add a terminator to the part's tag.
BBa_K1407007 RhlR CDS (no LVA tag) and terminator,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,terminator.With the RhlR protein existing,by adding PAI2,the part could be inducible and expresses RFP protein.Adding a promoter before the RhlR as you want.
BBa_K1407008 constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0034) and GFP,terminator.By adding PAI1,the part could be inducible and expresses GFP protein.
BBa_K1407009 RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP protein.
BBa_K1407010 constitutive production of LasR,LasR/PAI1 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI1,the part could be inducible and expresses RFP protein.
BBa_K1407011 constitutive promoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP protein.
BBa_K1407012 constitutive promoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.By adding PAI2,the part could be inducible and expresses RFP protein.
BBa_K1407013 onstitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and RFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducible and expresses RFP protein.
BBa_K1407014

constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0030)and RFP,terminator,constitutivepromoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI2/PAI1 exsits,the part could be inducible and expresses RFP protein.
BBa_K1407015

constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutivepromoter,RhlR protein generator ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducible and expresses GFP protein;When PAI2 exsits,the part could be inducible and expresses RFP protein.
BBa_K1407016

constitutive production of LasR,LasR/PAI1 Inducible promoter with RBS(B0034)and GFP,terminator,constitutivepromoter,RhlR protein generator(LVA-) ,RhlR/PAI2 inducible promoter with RBS (B0030) and RFP,Terminator.When PAI1 exsits,the part could be inducible and expresses GFP protein;When PAI2 exsits,the part could be inducible and expresses RFP protein.
Sensor BBa_K1407026

The recA promoter can response the radiation.Radiation will start the sos response .The recA protein is the important part of the sos response system.

Sequence and Features
BBa_K1407027 Toggle switch system with pRecA-LacI-pLac-LexA
BBa_K1407031 Lactose or IPTG inducible RFP reporter
BBa_K1407099 The recA promoter can response the radiation.Radiation will start the sos response .The recA protein is the important part of the sos response system. From BL21
Ba_K1407036

Lux pL controlled LuxR with lux pRautoinducingLuxI(lva tag)- AHL-GFP

amplifying GFP reporter

 

 

 

Part Collection

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