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Team:Bordeaux/NotebookAugust5
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Latest revision as of 03:20, 18 October 2014
5th August
Molecular biology
- Chewback: PCR step 1
ELP-40 with oligo ELP-40 forward and reverse -
Results of transformation :
pSB1C3+RFP, 200μl BL21: 0 colonies
pSB1C3+RFP, 20μl BL21: 0 colonies
pSB1C3+RFP, 2μl BL21: 0 colonies
ELP pUC57, 300μl BL21: 2 colonies
ELP pUC57, 300μl DH5α: 2 colonies
RLP pUC57, 300μl BL21: 97 colonies
RLP pUC57, 300μl BL21: 162 colonies
SLP pUC57, 300μl BL21: 9 colonies
SLP pUC57, 300μl DH5α: 15 colonies
- Try to make our biobricks ELP-40 and -60
Production
Production of ELP-40 and -60
