"
Team:NEFU China/Biosafety
From 2014.igem.org
| (3 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
<link rel="stylesheet" href="https://2014.igem.org/Team:NEFU_China/bootstrap.min?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2014.igem.org/Team:NEFU_China/bootstrap.min?action=raw&ctype=text/css"> | ||
<link rel="stylesheet" href="https://2014.igem.org/Team:NEFU_China/zmnetworks?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2014.igem.org/Team:NEFU_China/zmnetworks?action=raw&ctype=text/css"> | ||
| + | <style>#content .content p{text-align:justify;}</style> | ||
<script src="//code.jquery.com/jquery-1.11.0.min.js"></script> | <script src="//code.jquery.com/jquery-1.11.0.min.js"></script> | ||
<div class="container-fluid" id="p1"> | <div class="container-fluid" id="p1"> | ||
| Line 171: | Line 172: | ||
<td><p> BBa_K1509003 </p></td> | <td><p> BBa_K1509003 </p></td> | ||
<td><p> A strong </p> | <td><p> A strong </p> | ||
| - | <p> | + | <p> constitutive promoter </p></td> |
<td><p> Synthesized by BGI Tech </p></td> | <td><p> Synthesized by BGI Tech </p></td> | ||
<td><p> regulate CDS7 </p> | <td><p> regulate CDS7 </p> | ||
| Line 192: | Line 193: | ||
If you have any questions, please contact us. </li> | If you have any questions, please contact us. </li> | ||
</ul> | </ul> | ||
| + | <div class="footer"> | ||
| + | Team: NEFU_China<br> | ||
| + | Email: yichengzhao@live.cn<br> | ||
| + | Northeast Forestry University, Harbin, China | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 198: | Line 204: | ||
</div> | </div> | ||
</div> | </div> | ||
| + | <a href="http://www.zhaomunetworks.com" class="scrollup" style="display: none;">Scroll</a> | ||
<script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/js/bootstrap.min.js"></script> | <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.2.0/js/bootstrap.min.js"></script> | ||
<script> | <script> | ||
| Line 236: | Line 243: | ||
var top = document.documentElement.scrollTop ? document.documentElement.scrollTop : document.body.scrollTop; | var top = document.documentElement.scrollTop ? document.documentElement.scrollTop : document.body.scrollTop; | ||
var width = $(".left").width(); | var width = $(".left").width(); | ||
| - | + | if(top>=0){ | |
$(".fixed-nav").addClass("fixed-nav-fixed").width(width); | $(".fixed-nav").addClass("fixed-nav-fixed").width(width); | ||
}else{ | }else{ | ||
$(".fixed-nav").removeClass("fixed-nav-fixed"); | $(".fixed-nav").removeClass("fixed-nav-fixed"); | ||
} | } | ||
| + | if (top > 100) { | ||
| + | $('.scrollup').fadeIn(); | ||
| + | } else { | ||
| + | $('.scrollup').fadeOut(); | ||
| + | } | ||
| + | |||
} | } | ||
| + | $('.scrollup').click(function(){ | ||
| + | $("html, body").animate({ scrollTop: 0 }, 600); | ||
| + | return false; | ||
| + | }); | ||
}) | }) | ||
</script> | </script> | ||
</html> | </html> | ||
Latest revision as of 03:20, 18 October 2014
Biosafety
Overview
All of NEFU_China members completed safety training before the start of our project. The programs about safety training can be found here: http://dept.nefu.edu.cn/life/list/?227_1.html.(Chinese version). The Laboratory Biosafety Manual published by WHO has provided practical guidance for us. We work in a BSL1 lab equipped well to meet the safety criteria.
Q&A Section
- Q1. Please describe the chassis orgamisms you will be using in your project.
-
Species name
Risk Group
Risk Group Source
Disease risk to humans?
1
Synechococcus elongates PCC7942
1
No
2
Bacillus subtilis
1
It’s widely spread in the environment. And it is used in various areas and beneficial to human.
No
3
E.coli (DH5α)
1
www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia
No
4
E.coli (Rosetta pLysS)
1
www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia
No
- Q2. List and describe all new parts you will be using in your project.
-
Part name
Natural function of part
How did you acquire it?
How will you use it?
1
BBa_K1509000
Coding SmtB
Using PCR to isolate it from
Synechococcus elongates
PCC7942
Bind and regulate
smtO/P
2
BBa_K1509001
A regulator which is affected by
SmtB protein
Using PCR to isolate it from
Synechococcus elongates
PCC7942
Detect the heavy
metal ions with part1
3
BBa_K1509002
Mainly responsible for
flocculent activity
Using PCR to isolate it from Bacillus subtilis
Flocculate bacteria
to collect nanocrystals
4
BBa_K1509003
A strong
constitutive promoter
Synthesized by BGI Tech
regulate CDS7
gene expression
- Q3. Risks to the safety and health of team members or others working in the lab?
- We worked in a BSL1 lab and all of us had received the safety training. We have worked out an emergency package.
- Q4. What safety training have you received?
- In July, we completed Laboratory Biosafety Manual, Chemical/Fire/Electrical safety trainings before the start of our wet lab work. You can get more information here:http://dept.nefu.edu.cn/life/list/?227_1.html(Chinese version) If you have any questions, please contact us.
- Q5. Risks to the security through malicious misuse by individuals, groups, or countries?
- The biological materials we used have no menace and we have effective measures to prevent someone from misusing.
- Q6. If your project moved from a small-scale lab study to become widely used as a commercial/industrial product, what new risks might arise? Also, what risks might arise if the knowledge you generate or the methods you develop became widely available?
- Our project includes special feature to reduce this risk. The flocculation module was designed to carry our project forward to commercial/industrial product.
- Q7. Does your country have national biosafety regulations or guidelines? If so, please provide a link to these regulations or guidelines if possible.
- Here you can see our national biosafety regulations and guidelines: http://www.stee.agri.gov.cn/biosafety(Chinese version) If you have any questions, please contact us.
