Team:Penn/Protocol

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Latest revision as of 03:18, 18 October 2014

University of Pennsylvania iGEM

E.Coli Specific Protocols

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Click here to view the Spec Sheet!

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1^[7]

Transformation Protocol 2^[5]

Penn iGEM Transformation Protocol

Plating Techniques

Materials and Methods

Determining Magnetic Properties of AMB-1

Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures

Optimizing Transformation

Growth of AMB-1 and E.Coli in Cadmium

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-	<!--AAAAAA-->
-	<ul id="nav">
-		<a href="https://2014.igem.org/Team:Penn"><li>Home</li></a>
-  		<li>Project
-  			<ul>
-      			<a href="https://2014.igem.org/Team:Penn/Overview"> <li>Overview</li> </a>
-     			<a href="https://2014.igem.org/Team:Penn/Magnetism"> <li>Magnetism</li> </a>
-     			<a href="https://2014.igem.org/Team:Penn/Microbio"> <li>Microbiology</li> </a>
-     			<a href="https://2014.igem.org/Team:Penn/Synbio"> <li>SynBio in AMB-1</li> </a>
-     			<a href="https://2014.igem.org/Team:Penn/CdTolerance"> <li>Cadmium Tolerance</li> </a>
-   			</ul>
-  		</li>
-  		<li>Human Practices
-    		<ul>
-      			 <a href="https://2014.igem.org/Team:Penn/Specsheet"><li>Spec Sheet</li></a>
-     			 <a href="https://2014.igem.org/Team:Penn/Outreach"><li>Outreach</li></a>
-     			 <a href="https://2014.igem.org/Team:Penn/Biomeme"><li>Biomeme</li></a>
-   			</ul>
- 		</li>
-  		<li>Notebook
-  			<ul>
-      			<a href="https://2014.igem.org/Team:Penn/Notebook"><li>Timeline</li></a>
-      			<a href="https://2014.igem.org/Team:Penn/Safety"><li>Safety</li></a>
-     			<a href="https://2014.igem.org/Team:Penn/Protocol"><li>Protocols</li></a>
-     			<a href="https://2014.igem.org/Team:Penn/Supplement"><li>Supplementary Materials</li></a>
-   			</ul>
-   		</li>
-  		<li>About
-  			<ul>
-      			<a href="https://2014.igem.org/Team:Penn/Team"><li>Team</li></a>
-                        <a href="https://2014.igem.org/Team:Penn/Achievements"><li>Achievements</li></a>
-     			<a href="https://2014.igem.org/Team:Penn/Sponsors"><li>Sponsors</li></a>
-     			<a href="https://2014.igem.org/Team:Penn/Resources"><li>References</li></a>
-   			</ul>
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-<a href="https://2014.igem.org/Main_Page"> <img style="margin-left:30px" src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="40px"></a>
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-		</div>
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-				<div style = "text-align: center; font-size: 24px;">Protocols</div></br>
+				<div style = "text-align: center;"><img width="300px" src="https://static.igem.org/mediawiki/2014/e/ec/Protocols-header.png"></div><br>
 			<div id="textbox">
 <h3>E.Coli Specific Protocols</h3>
-<p><a href="">Miniprep</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/7e/Mini_Prep.pdf">Miniprep</a></p>
-<p><a href="">Cloning PCR </a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/0/0c/Cloning_PCR_Procedures.pdf">Cloning PCR </a></p>
-<p><a href="">Colony PCR</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/1/15/Protocol_Colony.pdf">Colony PCR</a></p>
-<p><a href="">Gel Extraction</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c6/Gel_Extraction.pdf">Gel Extraction</a></p>
-<p><a href="">PCR Cleanup</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup</a></p>
-<p><a href="">Restriction Enzyme Digest</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/94/Protocol.pdf">Ligation</a></p>
-<p><a href="">Ligation</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup </a></p>
-<p><a href="">Transformation </a></p>
-<p><a href="">Innoculation</a></p>
 <h3>AMB-1 Specific Protocols</h3>
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 <a href="https://2014.igem.org/Team:Penn/Specsheet" style = "font-size: 15px; margin:0 auto; color:#fff"> Click here to view the Spec Sheet! </a></br>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a0/MSGM_Media_Preparation.pdf">MSGM Media Preparation </a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a0/MSGM_Media_Preparation.pdf">MSGM Media Preparation </a></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5e/EMSGM_Media_Preparation.pdf">EMSGM Media Preparation</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5e/EMSGM_Media_Preparation.pdf">EMSGM Media Preparation</a></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/8/8c/Inoculation.pdf">Inoculation of Aerobic Cultures</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/8/8c/Inoculation.pdf">Inoculation of Aerobic Cultures</a></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a3/Counting_Cell_Cultures.pdf">Counting Cell Cultures</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a3/Counting_Cell_Cultures.pdf">Counting Cell Cultures</a></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ef/Transformation_Protocol_1.pdf">Transformation Protocol 1</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ef/Transformation_Protocol_1.pdf">Transformation Protocol 1</a><sup>[7]</sup></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/9a/Transformation_Protocol_2.pdf">Transformation Protocol 2</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/9a/Transformation_Protocol_2.pdf">Transformation Protocol 2</a><sup>[5]</sup></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/77/Penn_iGEM_Transformation_Protocol.pdf">Penn iGEM Transformation Protocol</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/77/Penn_iGEM_Transformation_Protocol.pdf">Penn iGEM Transformation Protocol</a></p>
-<style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5d/Plating_Techniques.pdf">Plating Techniques</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5d/Plating_Techniques.pdf">Plating Techniques</a></p>
+<h3>Materials and Methods</h3>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/4/49/Determining_Magnetic_Properties_of_AMB.pdf">Determining Magnetic Properties of AMB-1</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c7/Determining_the_Correlation_between_OD600_and_Cell_Concentration_for_Aerobic_and_Anaerobic_Cultures.pdf">Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/6/6f/Optimizing_Transformation.pdf">Optimizing Transformation</a></p>
+<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a8/Determining_Cadmium_Tolerance_of_AMB.pdf">Growth of AMB-1 and E.Coli in Cadmium</a></p>
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