Team:Penn/Protocol

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<title>Penn iGEM Wiki 2014</title>
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<link href="https://98c43ce680a0761e7c3ad3c1d7ae34f6de6db886.googledrive.com/host/0B9QyOqpKYA2gdGV2aGFRMWh4aXM/protocol.css" rel="stylesheet" type="text/css">
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<title>University of Pennsylvania iGEM</title>
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<div style = "text-align: center;"><img width="300px" src="https://static.igem.org/mediawiki/2014/e/ec/Protocols-header.png"></div><br>
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<p><a href="http://www.upenn.edu">University of Pennsylvania</a> <span>iGEM 2014</span></p>
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<li><a href="https://2014.igem.org/Team:Penn/Protocol"><span class="menu_item">Wet lab</span></a>
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                        <li><a href="https://2014.igem.org/Team:Penn/Protocol">Protocol</a></li>
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<h3>E.Coli Specific Protocols</h3>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/7e/Mini_Prep.pdf">Miniprep</a></p>
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<p id="title">Protocols</p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/0/0c/Cloning_PCR_Procedures.pdf">Cloning PCR </a></p>
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<p>Browse/download our protocols below</p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/1/15/Protocol_Colony.pdf">Colony PCR</a></p>
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<p class="protocol_title"><a href="">Cloning PCR</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c6/Gel_Extraction.pdf">Gel Extraction</a></p>
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<p class="protocol_title"><a href="">Colony PCR</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup</a></p>
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<p class="protocol_title"><a href="">LB media</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/94/Protocol.pdf">Ligation</a></p>
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<p class="protocol_title"><a href="">PCR clean-up</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c1/PCR_Cleanup.pdf">PCR Cleanup </a></p>
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<p class="protocol_title"><a href="">Gel Electrophoresis</a></p>
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<p class="protocol_title"><a href="">Gel Extraction</a></p>
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<p class="protocol_title"><a href="">AMB-1 Electroporation</a></p>
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<p class="protocol_title"><a href="https://static.igem.org/mediawiki/2014/1/10/Bacteria_Cadmium_Tolerance.pdf">Bacteria Cadmium Tolerance</a></p>
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<h3>AMB-1 Specific Protocols</h3>
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<p style = "text-align: left; text-indent:0px"> Our protocols for working with AMB-1 have been outlined below. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.</p>
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<a href="https://2014.igem.org/Team:Penn/Specsheet" style = "font-size: 15px; margin:0 auto; color:#fff"> Click here to view the Spec Sheet! </a></br>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ea/Glycerol_Stock_of_Anaerobic_AMB-1.pdf">Glycerol Stock</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a0/MSGM_Media_Preparation.pdf">MSGM Media Preparation </a></p>
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<object id="follow_me" width='101' height='48'><embed src='http://www.socialbuttonmaker.com/Swfs/45da3933-8326-4170-89c0-10dea0659e98/button.swf' type='application/x-shockwave-flash' width='101' height='48' loop='false' wmode='transparent'><noscript><a href='http://soccerisland.info/'>island soccer news</a></noscript></embed><img src='' width=0 height=0 id='flashbutton'/><script type='text/javascript' src='http://www.socialbuttonmaker.com/Scripts/FlashButton.js'></script></object>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5e/EMSGM_Media_Preparation.pdf">EMSGM Media Preparation</a></p>
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<p>Penn iGEM  &copy; 2014</p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/8/8c/Inoculation.pdf">Inoculation of Aerobic Cultures</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a3/Counting_Cell_Cultures.pdf">Counting Cell Cultures</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/e/ef/Transformation_Protocol_1.pdf">Transformation Protocol 1</a><sup>[7]</sup></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/9/9a/Transformation_Protocol_2.pdf">Transformation Protocol 2</a><sup>[5]</sup></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/7/77/Penn_iGEM_Transformation_Protocol.pdf">Penn iGEM Transformation Protocol</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/5/5d/Plating_Techniques.pdf">Plating Techniques</a></p>
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<h3>Materials and Methods</h3>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/4/49/Determining_Magnetic_Properties_of_AMB.pdf">Determining Magnetic Properties of AMB-1</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/c/c7/Determining_the_Correlation_between_OD600_and_Cell_Concentration_for_Aerobic_and_Anaerobic_Cultures.pdf">Determining Correlation between OD600 and Cell Concentration for Aerobic and Anaerobic Cultures</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/6/6f/Optimizing_Transformation.pdf">Optimizing Transformation</a></p>
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<p style = "text-align: left; text-indent:0px"><a href="https://static.igem.org/mediawiki/2014/a/a8/Determining_Cadmium_Tolerance_of_AMB.pdf">Growth of AMB-1 and E.Coli in Cadmium</a></p>
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Latest revision as of 03:18, 18 October 2014

University of Pennsylvania iGEM