Team:ZJU-China/Safety

From 2014.igem.org

(Difference between revisions)
Line 25: Line 25:
<td>DH5-alpha</td>
<td>DH5-alpha</td>
<td>1</td>
<td>1</td>
-
<td>Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
+
<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
</td>
</td>
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<td>BL21(DE3)</td>
<td>BL21(DE3)</td>
<td>1</td>
<td>1</td>
-
<td>Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
+
<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
</tr>
</tr>
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<td>DH10-beta</td>
<td>DH10-beta</td>
<td>1</td>
<td>1</td>
-
<td>Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
+
<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
</tr>
</tr>
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<td>BW25113</td>
<td>BW25113</td>
<td>1</td>
<td>1</td>
-
<td>Yes.May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
+
<td>Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.</td>
</tr>
</tr>
</table>
</table>
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         <ol>
         <ol>
             <li><b>Lab Safety</b>
             <li><b>Lab Safety</b>
-
             <p>Zhe Jiang University has set a strict protocol to ensure the safety of laboratory. Students are required the wear protect equipments including lab-gown, long pants, gloves and so on while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.</p>
+
             <p>Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.</p>
             </li>
             </li>
             <li><b>Research Safety</b>
             <li><b>Research Safety</b>
-
             <p>All students involved hava been trained to operate equipments, reagents and strains in a safety way. We are not able to do ecperiment until pass the bio-safety examination.</p>
+
             <p>All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.</p>
             </li>
             </li>
             <li><b>Environment Safety</b>
             <li><b>Environment Safety</b>
-
             <p> All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don’t contain F plasmid, which guarantee our gene would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.</p>
+
             <p> All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.</p>
             </li>
             </li>
             <li><b>Reference</b>
             <li><b>Reference</b>

Revision as of 03:16, 18 October 2014


 

Species Strain NIH Risk Group Disease risk to human?
E.Coli(K12) DH5-alpha 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) BL21(DE3) 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) DH10-beta 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.
E.Coli(K12) BW25113 1 Yes.May cause irritation to skin, eyes, and respiratory tract; may affect kidneys.

 

 

Our Safety Protocol

  1. Lab Safety

    Zhejiang University has set a strict protocol to ensure the safety of laboratory. Students are required to wear protective equipments including lab-gown, long pants, gloves etc, while dealing with chemical and biological reagents. A room is specialized for DNA gel even we use ExRed replace EB as laboratory has a reasonable division.

  2. Research Safety

    All students involved have been trained to operate equipments, reagents and strains in a safe way. We are not able to do ecperiments until we pass the bio-safety examination.

  3. Environment Safety

    All the strains DH5-alpha, BL21(DE3),DH10-beta and BW25113 we used don't contain F plasmid, which guarantees that our genes would not spread to other bacterial in environment. The useless bacterial colonies will be sterilized before abandoned.

  4. Reference

    http://www.absa.org/riskgroups/bacteriasearch.php?genus=Escherichia

    http://www.absa.org/riskgroups/