Team:Technion-Israel

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<title>Safie by Technion-Israel</title>
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<ul>
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<li id="parent"><a href="https://2014.igem.org/Team:Technion-Israel">Home</a></li>
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<a href="Project.html">Project</a>
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<a href="https://2014.igem.org/Team:Technion-Israel/Project">Project</a>
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<li><a href="Project.html#idea">The idea</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#idea">The idea</a></li>
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<li><a href="Project.html#HK">Histedin kinase</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#howitworks">How it works</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#alpha">Alpha System</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#beta">Beta System</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#rna">RNA splint</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#azo">Azobenzene</a></li>
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                                        <li><a href="Project.html#methods">Methods &amp; protocols</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#hk">Histidine Kinase</a></li>
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<li><a href="Project.html#notebook">Lab notebook</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#newmethod">New Standard</a></li>
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<li><a href="Project.html#safety">Safety</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#protocol">Protocols</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#notebook">Lab Notebook</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Project#safety">Safety</a></li>
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<li id="parent"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling">Modeling</a>
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                                 <a href="HP.html">Human Practice</a>
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  <ul class="sub1">
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#whyworks">Why it should work</a></li>
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<li><a href="#">SynBio in 3 languages</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#whyfail">Why it should fail</a></li>
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<li><a href="#">iGEM high-schools</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#splint">RNA Splint</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Modeling#biofilm">Synthetic Biofilm<br>Formation</a></li>
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  </ul>
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<li id="parent"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments">Experiments</a>
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  <ul class="sub1">
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#gate1">Gate 1</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#gate2">Gate 2</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#rna">RNA Splint</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#pompc">Pompc-RFP</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#taz">TaZ</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#mcherry">mCherry</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#amilcp">amilCP</a></li>
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    <li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Experiments#azo">Azobenzene</a></li>
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<a href="https://2014.igem.org/Team:Technion-Israel/HP">Policy &amp; Practices</a>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#3lang">SynBio in 3 Languages</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#highschool">iGEM High Schools</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#lectures">SynBio Lectures</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#collab">Collaborations</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/HP#art">Art</a></li>
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                                 <a href="team.html">The Team</a>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Team#gallery">Gallery</a></li>
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<li id="child1"><a href="https://igem.org/Team.cgi?id=1343" target="_blank">Official Team<br>Profile</a></li>
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<li id="child1"><a href="https://www.facebook.com/iGEM2014.Technion" target="_blank">Official Facebook<br>Page</a></li>
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                                 <a href="support.html">Special Thanks</a>
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                                        <li><a href="#">Attributions</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Support#attributions">Attributions</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Support#sponsors">Sponsors</a></li>
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                                 <a href="Judging.html">Judging</a>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Judging#biobrick">BioBricks</a></li>
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<li id="child1"><a href="https://2014.igem.org/Team:Technion-Israel/Judging#results">Results</a></li>
                                     </ul>
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<div class="title">The Project</div>
<div class="title">The Project</div>
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<p class="style2"><strong>We are developing a low concentration bio-detector.</strong><br></p>
<p class="style2"><strong>We are developing a low concentration bio-detector.</strong><br></p>
                     <p class="style3">The system is based on an intricate <strong>gate construct</strong>,
                     <p class="style3">The system is based on an intricate <strong>gate construct</strong>,
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<h3 class="icon fa-lightbulb-o">The idea</h3>
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<h3 class="icon fa-lightbulb-o">The Idea</h3>
<p>Read about our vision for our project. Find out more about the idea and where some R&amp;D can take us</p>
<p>Read about our vision for our project. Find out more about the idea and where some R&amp;D can take us</p>
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<h3 class="icon fa-circle-o-notch">Histidine Kinase</h3>
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<h3 class="icon fa-exclamation">How it Works</h3>
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<p>Read how the Histidine Kinase system in E.coli can be used for different applications and substances</p>
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<p>Find out how we planed to achieve our goal for low concentration detection of different materials</p>
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<li><a href="#" class="button style1 big">Read more</a></li>
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<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#howitworks" class="button style1 big">Read more</a></li>
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<h3 class="icon fa-toggle-on">Gate construct</h3>
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<h3 class="icon fa-gear">Alpha System</h3>
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<p>Explore our genetic logic gates in detail, what happens in the system and how it can detect low concentrations</p>
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<p>Explore our first genetic logic gates design in detail, what happens in the system and how it can detect low concentrations</p>
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<h3 class="icon fa-flask">Azobenzene</h3>
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<h3 class="icon fa-cogs">Beta System</h3>
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<p>Read about our pioneering method for bacterial aggregation using organically synthesized Azobenzene molecules</p>
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<p>Read about our second design of logic gates meant to reduce noise and help with more accurate detection</p>
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<section>
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<h3 class="icon fa-laptop">modeling</h3>
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<h3 class="icon fa-expand">RNA Splint</h3>
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<p>Check out our modeling for low concentration detection and bacterial aggregation</p>
+
<p>Find out about our plan to split any desired gene sequence to produce it under 2 different promoters and have the full protein self-assemble</p>
<ul class="actions actions-centered">
<ul class="actions actions-centered">
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</ul>
</ul>
</section>
</section>
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                        <div class="6u">
 +
<section>
 +
<h3 class="icon fa-flask">Azobenzene</h3>
 +
<p>Read about our pioneering method for bacterial aggregation using organically synthesized Azobenzene molecules</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#azo" class="button style1 big">Read more</a></li>
 +
</ul>
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<h3 class="icon fa-bar-chart">Experiments</h3>
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<h3 class="icon fa-tencent-weibo">Histidine Kinase</h3>
-
<p>Find out about the experiments we preformed and see the results. Read more about future experiments we planned</p>
+
<p>Read how the Histidine Kinase system in E.coli can be used for different applications and substances</p>
 +
<ul class="actions actions-centered">
 +
<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#hk" class="button style1 big">Read more</a></li>
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</ul>
 +
                                </section>
 +
</div>
 +
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 +
<section>
 +
<h3 class="icon fa-barcode">New Standard</h3>
 +
<p>Explore a new standard for faster DNA assembly based on<br> Gibson assembly</p>
<ul class="actions actions-centered">
<ul class="actions actions-centered">
-
<li><a href="#" class="button style1 big">Read more</a></li>
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<li><a href="https://2014.igem.org/Team:Technion-Israel/Project#newmethod" class="button style1 big">Read more</a></li>
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<h3 class="icon fa-wrench">Methods &amp; protocols</h3>
+
<h3 class="icon fa-laptop">Modeling</h3>
-
<p>Read about the methods we used in the lab and download the protocols</p>
+
<p>Check out our extensive modeling for low concentration detection and bacterial aggregation<br></p>
<ul class="actions actions-centered">
<ul class="actions actions-centered">
-
<li><a href="#" class="button style1 big">Read more</a></li>
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<p>You can find out what we did the whole summer in the lab in each sub-project</p>
+
<p>Find out about the experiments we preformed and see the results. Read more about future experiments we planned</p>
<ul class="actions actions-centered">
<ul class="actions actions-centered">
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<section id="features">
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<h2>In our Human Practices project we focus on 3 principles:</h2>
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<h2>In our Policy &amp; Practices project we focus on 3 principles:</h2>
<p>Encouraging independent thinking and creativity among teens and youth<br>
<p>Encouraging independent thinking and creativity among teens and youth<br>
Progressing Arab-Jewish relations through the medium of science<br>
Progressing Arab-Jewish relations through the medium of science<br>
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Representing Israel as ambassadors in the worldwide competition of iGEM</p>
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Representing Israel as ambassadors in the international iGEM competition</p>
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<h3 class="icon fa-language">SynBio in 3 languages</h3>
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<h3 class="icon fa-language">SynBio in 3 Languages</h3>
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<p>All of our human practice activities are related to our main concept of SynBio in 3 languages. Each activity was designed to include all members of Israeli society, and was held in Hebrew, Arabic and English.</p>
+
<p><br>All of our Policy &amp; Practices activities are related to our main concept of SynBio in 3 languages. Each activity was designed to include all members of Israeli society.</p>
                                         <ul class="actions actions-centered">
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<li><a href="#" class="button style1 big">Read more</a></li>
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<h3 class="icon fa-graduation-cap">iGEM high-schools</h3>
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<h3 class="icon fa-graduation-cap">iGEM High Schools</h3>
-
<p>We have launched the first iGEM high-school team in Israel! We designed detaild lesson plans, raised the money for the team and found facilities. The first group will start working this winter.</p>
+
<p>We have launched the first iGEM High School team in Israel! We designed detaild lesson plans and found facilities for the project. The first group will start working for the 2015 competition.<br></p>
    <ul class="actions actions-centered">
    <ul class="actions actions-centered">
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<li><a href="#" class="button style1 big">Read more</a></li>
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<li><a href="https://2014.igem.org/Team:Technion-Israel/HP#highschool" class="button style1 big">Read more</a></li>
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                                     </section>
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<section>
<h3 class="icon fa-user">SynBio Lectures</h3>
<h3 class="icon fa-user">SynBio Lectures</h3>
-
<p>We have build lectures for young and older kids on Synthetic biology in space and in water, for our activities and for your free use.</p>
+
<p>We have created detailed lesson plans including slideshows for children of all ages about Synthetic Biology in space and in water.  We used these lesson plans for our activities and they are available for your free use.</p>
    <ul class="actions actions-centered">
    <ul class="actions actions-centered">
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<li><a href="#" class="button style1 big">Read more</a></li>
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<h3 class="icon fa-users">Collaborations</h3>
<h3 class="icon fa-users">Collaborations</h3>
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<p>In the iGEM spirit we collaborated with the Paris_Bettencourt team and BGU_Israel in a few projects.</p>
+
<p><br>In the iGEM spirit we collaborated with the Paris_Bettencourt team on a few projects including a weekly newsletter, and with BGU_Israel on an experiment.<br></p>
    <ul class="actions actions-centered">
    <ul class="actions actions-centered">
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<li><a href="#" class="button style1 big">Read more</a></li>
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<div class="title">The Team</div>
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<h2>We Would like to thank the following sponsers:</h2>
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<h2>We would like to thank the following sponsors:</h2>
<p>
<p>
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<h3><a href="http://lokey.technion.ac.il/Lokey/index.asp" target="_blank">The Lorry I. Lokey center</a></h3>
<h3><a href="http://lokey.technion.ac.il/Lokey/index.asp" target="_blank">The Lorry I. Lokey center</a></h3>
<!--<p>Lokey Interdisciplinary Center for Life Sciences and Engineering</p>-->
<!--<p>Lokey Interdisciplinary Center for Life Sciences and Engineering</p>-->
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<a href="http://www.technion.ac.il/en/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/6/6c/Technion-Israel-technion.jpg" alt="" /></a>
<h3><a href="http://www.technion.ac.il/en/" target="_blank">Technion</a></h3>
<h3><a href="http://www.technion.ac.il/en/" target="_blank">Technion</a></h3>
<!--<p>Technion - Israel Institute of Technology -  public research university in Haifa, Israel.</p>-->
<!--<p>Technion - Israel Institute of Technology -  public research university in Haifa, Israel.</p>-->
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<a href="http://www.sigmaaldrich.com/israel.html" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/4/43/Technion-Israel-sigma.jpg" alt="" /></a>
<h3><a href="http://www.sigmaaldrich.com/israel.html" target="_blank">Sigma-Aldrich, Israel</a></h3>
<h3><a href="http://www.sigmaaldrich.com/israel.html" target="_blank">Sigma-Aldrich, Israel</a></h3>
<!--<p>Sigma-Aldrich is a leading life science and high technology company.</p>-->
<!--<p>Sigma-Aldrich is a leading life science and high technology company.</p>-->
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<a href="http://www.labotal.co.il/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/2/28/Technion-Israel-labotal.jpg" alt="" /></a>
<h3><a href="http://www.labotal.co.il/" target="_blank">Labotal</a></h3>
<h3><a href="http://www.labotal.co.il/" target="_blank">Labotal</a></h3>
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<a href="http://www.bioanalytics.co.il/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/7/78/Technion-Israel-bioan.jpg" alt="" /></a>
<h3><a href="http://www.bioanalytics.co.il/" target="_blank">BioAnalytics</a></h3>
<h3><a href="http://www.bioanalytics.co.il/" target="_blank">BioAnalytics</a></h3>
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<a href="http://www.danyel.co.il/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/f/f4/Technion-Israel-dani.jpg" alt="" /></a>
<h3><a href="http://www.danyel.co.il/" target="_blank">DanyelBiotech</a></h3>
<h3><a href="http://www.danyel.co.il/" target="_blank">DanyelBiotech</a></h3>
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<a href="http://www.hylabs.co.il/" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/a/a3/Technion-Israel-hy.jpg" alt="" /></a>
<h3><a href="http://www.hylabs.co.il/" target="_blank">Hy-labs</a></h3>
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<a href="http://www.headstart.co.il/project.aspx?id=10603&lan=en-US" target="_blank" class="image featured"><img src="https://static.igem.org/mediawiki/2014/f/f4/Technion-Israel-headstart.jpg" alt="" /></a>
<h3><a href="http://www.headstart.co.il/project.aspx?id=10603&lan=en-US">Headstart supporters</a></h3>
<h3><a href="http://www.headstart.co.il/project.aspx?id=10603&lan=en-US">Headstart supporters</a></h3>
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<li><a href="https://www.facebook.com/iGEM2014.Technion">Visit our Facebook page</a></li><li>&copy; All rights reserved</li><!--<li>&copy; Technion-Israel</li>--><li>Design: <a href="http://html5up.net">HTML5 UP</a></li><li>igem14il@gmail.com</li>
 
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<li><a href="https://www.facebook.com/iGEM2014.Technion" target="_blank">Visit our Facebook page</a></li><li>&copy; All rights reserved</li><!--<li>&copy; Technion-Israel</li>--><li>Design: <a href="http://html5up.net" target="_blank">HTML5 UP</a> &amp; Technion_Israel Team</li><li>igem14il@gmail.com</li>
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Latest revision as of 03:12, 18 October 2014

Safie by Technion-Israel

The Project

We are developing a low concentration bio-detector.

The system is based on an intricate gate construct, utilizes quorum-sensing and an altered two-component signalling system. In addition, groundbreaking organic synthesis of a photo-switching molecule – Azobenzene – is used in a pioneering attempt to mimic biofilm formation.
We have planned and built a low concentration detector using these elements.



The Idea

Read about our vision for our project. Find out more about the idea and where some R&D can take us

How it Works

Find out how we planed to achieve our goal for low concentration detection of different materials

Alpha System

Explore our first genetic logic gates design in detail, what happens in the system and how it can detect low concentrations

Beta System

Read about our second design of logic gates meant to reduce noise and help with more accurate detection

RNA Splint

Find out about our plan to split any desired gene sequence to produce it under 2 different promoters and have the full protein self-assemble

Azobenzene

Read about our pioneering method for bacterial aggregation using organically synthesized Azobenzene molecules

Histidine Kinase

Read how the Histidine Kinase system in E.coli can be used for different applications and substances

New Standard

Explore a new standard for faster DNA assembly based on
Gibson assembly

Modeling

Check out our extensive modeling for low concentration detection and bacterial aggregation

Experiments

Find out about the experiments we preformed and see the results. Read more about future experiments we planned

Policy & Practices

In our Policy & Practices project we focus on 3 principles:

Encouraging independent thinking and creativity among teens and youth
Progressing Arab-Jewish relations through the medium of science
Representing Israel as ambassadors in the international iGEM competition

SynBio in 3 Languages


All of our Policy & Practices activities are related to our main concept of SynBio in 3 languages. Each activity was designed to include all members of Israeli society.

iGEM High Schools

We have launched the first iGEM High School team in Israel! We designed detaild lesson plans and found facilities for the project. The first group will start working for the 2015 competition.

SynBio Lectures

We have created detailed lesson plans including slideshows for children of all ages about Synthetic Biology in space and in water. We used these lesson plans for our activities and they are available for your free use.

Collaborations


In the iGEM spirit we collaborated with the Paris_Bettencourt team on a few projects including a weekly newsletter, and with BGU_Israel on an experiment.

The Team
Special Thanks

Retrieved from "http://2014.igem.org/Team:Technion-Israel"